Restriction map for
pFN21AE1149
(Circular) (Six-base) MAPSORT of: pFN21AE1149.seq Check: 3278 from: 1 to: 6052
pFN21AE1149 6052 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2246 2246
Size: 6052
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2524 2937 3042 4115 5499 5767 1632
Size: 892 413 105 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1073 892 413 268 105
AfeI AGC'GCT
Cuts at: 2945 2945
Size: 6052
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3228 1184
Size: 2044 4008
AlwNI CAG_nnn'CTG
Cuts at: 3795 5367 3795
Size: 1572 4480
ApaI G_GGCC'C
Cuts at: 2043 2043
Size: 6052
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2375 3377 1870
Size: 73 226 206 1002 4545
Fragments arranged by size:
4545 1002 226 206 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2433 2466 2433
Size: 33 6019
BamHI G'GATC_C
Cuts at: 2062 3382 3749 2062
Size: 1320 367 4365
Fragments arranged by size:
4365 1320 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2206 2299 2427 2662
Size: 325 217 627 301 118 93 128 235
Cuts at: 2662 2928 3373 4828 618
Size: 266 445 1455 1842
Fragments arranged by size:
1842 1455 627 445 325 301 266 235
217 128 118 93
BanII G_rGCy'C
Cuts at: 1391 2043 2646 3371 1391
Size: 652 603 725 4072
Fragments arranged by size:
4072 725 652 603
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 928
Size: 971 824 4257
Fragments arranged by size:
4257 971 824
BbvCI CC'TCA_GC
Cuts at: 3066 3066
Size: 6052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 2292 4663
Size: 107 122 71 631 605 467 153 2371
Cuts at: 4663 136
Size: 1525
Fragments arranged by size:
2371 1525 631 605 467 153 122 107
71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2144 2444 4113 5269 1790
Size: 253 101 300 1669 1156 2573
Fragments arranged by size:
2573 1669 1156 300 253 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3783 4741 442
Size: 416 53 83 399 24 2366 958 1753
Fragments arranged by size:
2366 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2279 2371 2420 2639 2987 3298 4712
Size: 887 153 92 49 219 348 311 1414
Cuts at: 4712 1239
Size: 2579
Fragments arranged by size:
2579 1414 887 348 311 219 153 92
49
Bpu10I CC'TnA_GC
Cuts at: 2081 3066 2081
Size: 985 5067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4177 5042 5340 4177
Size: 865 298 4889
Fragments arranged by size:
4889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4715 882
Size: 617 3216 2219
Fragments arranged by size:
3216 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4485 4883 5157 5304 5715 4485
Size: 398 274 147 411 4822
Fragments arranged by size:
4822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2411 2441 2979
Size: 30 165 30 822 30 156 30 538
Cuts at: 2979 3009 1178
Size: 30 4221
Fragments arranged by size:
4221 822 538 165 156 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2432 3023 3041 3083 1186
Size: 1243 3 591 18 42 4155
Fragments arranged by size:
4155 1243 591 42 18 3
BseYI C'CCAG_C
Cuts at: 2295 2380 2946 5255 5751 2295
Size: 85 566 2309 496 2596
Fragments arranged by size:
2596 2309 566 496 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 1163
Size: 573 465 5014
Fragments arranged by size:
5014 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 4113 4198 5269 2397
Size: 1716 85 1071 3180
Fragments arranged by size:
3180 1716 1071 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2144 2397 2444 2646 3371 4113
Size: 399 253 101 253 47 202 725 742
Cuts at: 4113 4198 5269 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 253 253 202
101 85 47
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2246 3407 844
Size: 1402 1161 3489
Fragments arranged by size:
3489 1402 1161
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2133 2150 2424 4696 4883 1700
Size: 110 281 42 17 274 2272 187 2869
Fragments arranged by size:
2869 2272 281 274 187 110 42 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 2324 4112 5124 2324
Size: 1788 1012 3252
Fragments arranged by size:
3252 1788 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2062 3382 3749 4134 4151 5603 5689 5701
Size: 90 1320 367 385 17 1452 86 12
Cuts at: 5701 6047 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 90 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2454 2546 2454
Size: 92 5960
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2043 2210 2460 513
Size: 552 939 23 16 167 250 4105
Fragments arranged by size:
4105 939 552 250 167 23 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2303 3568 4362 4382 2303
Size: 1265 794 20 3973
Fragments arranged by size:
3973 1265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DraIII CAC_nnn'GTG
Cuts at: 2935 2935
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2408 2475
Size: 54 1425 50 275 18 318 260 67
Cuts at: 2475 3499 4388 6017 8
Size: 1024 889 1629 43
Fragments arranged by size:
1629 1425 1024 889 318 275 260 67
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2622 2976 3018 3114 3982 1353
Size: 1190 79 354 42 96 868 3423
Fragments arranged by size:
3423 1190 868 354 96 79 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2279 2371 2420 2524 2639 2937
Size: 393 494 153 92 49 104 115 298
Cuts at: 2937 2987 3042 3298 4115 4712 5499 5767 1239
Size: 50 55 256 817 597 787 268 1524
Fragments arranged by size:
1524 817 787 597 494 393 298 268
256 153 115 104 92 55 50 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2435
Size: 6052
EcoO109I rG'GnC_Cy
Cuts at: 2039 2335 2355 2039
Size: 296 20 5736
Fragments arranged by size:
5736 296 20
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 6052
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 2318 2425 2947 5199 1678
Size: 640 107 522 2252 2531
Fragments arranged by size:
2531 2252 640 522 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 3313 3345 4693
Size: 32 135 32 911 32 798 32 1348
Cuts at: 4693 4725 4767 4799 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1348 911 798 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3318 5166 5350 1305
Size: 2013 1848 184 2007
Fragments arranged by size:
2013 2007 1848 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2410 2477 10
Size: 54 1425 343 578 67 3585
Fragments arranged by size:
3585 1425 578 343 67 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 4010 4369 4528 518
Size: 648 1707 1137 359 159 2042
Fragments arranged by size:
2042 1707 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2578 2864 3175 3276 3468 4145 5293
Size: 606 366 286 311 101 192 677 1148
Cuts at: 5293 5538 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 606 366 311 286 245
192 101
NaeI GCC'GGC
Cuts at: 2093 2135 2152 2093
Size: 42 17 5993
Fragments arranged by size:
5993 42 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4561
Fragments arranged by size:
4561 939 552
NdeI CA'TA_TG
Cuts at: 387 2817 387
Size: 2430 3622
NgoMIV G'CCGG_C
Cuts at: 2091 2133 2150 2091
Size: 42 17 5993
Fragments arranged by size:
5993 42 17
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 3061 3410 4955 1132
Size: 97 1832 349 1545 2229
Fragments arranged by size:
2229 1832 1545 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2578 2581 1108
Size: 1470 3 4579
Fragments arranged by size:
4579 1470 3
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2183 2215 4121 4153 5634 5666 2183
Size: 32 1906 32 1481 32 2569
Fragments arranged by size:
2569 1906 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PspOMI G'GGCC_C
Cuts at: 2039 2039
Size: 6052
PstI C_TGCA'G
Cuts at: 830 2283 2334 2674 3404 830
Size: 1453 51 340 730 3478
Fragments arranged by size:
3478 1453 730 340 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2578 2864 3175 3276 2578
Size: 286 311 101 5354
Fragments arranged by size:
5354 311 286 101
SacII CC_GC'GG
Cuts at: 2213 2213
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SfcI C'TryA_G
Cuts at: 826 1045 2279 2330 2599 2670 3400 4535 5216
Size: 219 1234 51 269 71 730 1135 681
Cuts at: 5216 5407 826
Size: 191 1471
Fragments arranged by size:
1471 1234 1135 730 681 269 219 191
71 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 2424 1700
Size: 724 5328
SmaI CCC'GGG
Cuts at: 1872 2171 3379 1872
Size: 299 1208 4545
Fragments arranged by size:
4545 1208 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4156 5057 5319 820
Size: 197 926 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3061 3410 1132
Size: 1929 349 3774
Fragments arranged by size:
3774 1929 349
SspI AAT'ATT
Cuts at: 5 52 3145 3976 5
Size: 47 3093 831 2081
Fragments arranged by size:
3093 2081 831 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 3235 3491 513
Size: 552 939 114 1117 256 3074
Fragments arranged by size:
3074 1117 939 552 256 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 4079 4096 4249 4434 4879 325
Size: 1255 707 1792 17 153 185 445 1498
Fragments arranged by size:
1792 1498 1255 707 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4298 6010 96
Size: 274 80 33 51 1443 2321 1712 138
Fragments arranged by size:
2321 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2411 2443 2979 3011 3130 3162 6012
Size: 32 154 32 536 32 119 32 2850
Cuts at: 6012 6044 2225
Size: 32 2233
Fragments arranged by size:
2850 2233 536 154 119 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 2710 3388 2710
Size: 678 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 2169 3377 1870
Size: 299 1208 4545
Fragments arranged by size:
4545 1208 299
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI