Restriction map for
pFN21AE1148
(Circular) (Six-base) MAPSORT of: pFN21AE1148.seq Check: 3082 from: 1 to: 6091
pFN21AE1148 6091 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2154 2154
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 4154 5538 5806 1632
Size: 835 1687 1384 268 1917
Fragments arranged by size:
1917 1687 1384 835 268
AfeI AGC'GCT
Cuts at: 2157 2157
Size: 6091
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 2703 1166
Size: 1537 4554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2280 2594 1184
Size: 1096 314 4681
Fragments arranged by size:
4681 1096 314
AlwNI CAG_nnn'CTG
Cuts at: 2146 2842 2914 2971 3260 3834 5406 2146
Size: 696 72 57 289 574 1572 2831
Fragments arranged by size:
2831 1572 696 574 289 72 57
ApaI G_GGCC'C
Cuts at: 2013 2206 2252 2534 3034 3160 3184 2013
Size: 193 46 282 500 126 24 4920
Fragments arranged by size:
4920 500 282 193 126 46 24
ApaLI G'TGCA_C
Cuts at: 2864 4148 5304 2864
Size: 1284 1156 3651
Fragments arranged by size:
3651 1284 1156
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2853 2988 3416 1870
Size: 73 910 135 428 4545
Fragments arranged by size:
4545 910 428 135 73
AvrII C'CTAG_G
Cuts at: 2741 2741
Size: 6091
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2206 2252 2534 2751 3034 3160 3184
Size: 622 193 46 282 217 283 126 24
Cuts at: 3184 3410 1391
Size: 226 4072
Fragments arranged by size:
4072 622 283 282 226 217 193 126
46 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5120
BbvCI CC'TCA_GC
Cuts at: 2462 2976 3162 2462
Size: 514 186 5391
Fragments arranged by size:
5391 514 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2019 2053 4282 4316 1903
Size: 34 82 34 2229 34 3678
Fragments arranged by size:
3678 2229 82 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2764 2838 3125
Size: 107 122 71 631 605 1092 74 287
Cuts at: 3125 3155 4702 136
Size: 30 1547 1525
Fragments arranged by size:
1547 1525 1092 631 605 287 122 107
74 71 30
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2152 2517 3508 2152
Size: 365 991 4735
Fragments arranged by size:
4735 991 365
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2124 2206 2252 2534 2868 3034 3160
Size: 223 111 82 46 282 334 166 126
Cuts at: 3160 3184 4152 5308 1790
Size: 24 968 1156 2573
Fragments arranged by size:
2573 1156 968 334 282 223 166 126
111 82 46 24
BmgBI CAC'GTC
Cuts at: 1318 2281 1318
Size: 963 5128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3029 3187 3822
Size: 416 53 83 399 24 1612 158 635
Cuts at: 3822 4780 442
Size: 958 1753
Fragments arranged by size:
1753 1612 958 635 416 399 158 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2429 2948 4751 1239
Size: 968 222 519 1803 2579
Fragments arranged by size:
2579 1803 968 519 222
Bpu10I CC'TnA_GC
Cuts at: 2462 2976 3162 2462
Size: 514 186 5391
Fragments arranged by size:
5391 514 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 4216 5081 5379 2255
Size: 1961 865 298 2967
Fragments arranged by size:
2967 1961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 2786 4754 882
Size: 617 849 438 1968 2219
Fragments arranged by size:
2219 1968 849 617 438
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3882 493
Size: 711 2001 677 2702
Fragments arranged by size:
2702 2001 711 677
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3013 3898 4280 4915
Size: 53 83 186 936 1480 885 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1480 1451 936 885 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2738 2768 1178
Size: 30 165 30 777 30 528 30 4501
Fragments arranged by size:
4501 777 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3126 1186
Size: 1940 4151
BseYI C'CCAG_C
Cuts at: 2212 2527 2533 2845 2982 3153 3235 3253 3263
Size: 315 6 312 137 171 82 18 10
Cuts at: 3263 5294 5790 2212
Size: 2031 496 2513
Fragments arranged by size:
2513 2031 496 315 312 171 137 82
18 10 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2320 3056 1163
Size: 573 584 736 4198
Fragments arranged by size:
4198 736 584 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2518 2616 2688 2868 3042 4152 4237 5308 2518
Size: 98 72 180 174 1110 85 1071 3301
Fragments arranged by size:
3301 1110 1071 180 174 98 85 72
BsmI GAATG_Cn'
Cuts at: 2699 3602 3695 2699
Size: 903 93 5095
Fragments arranged by size:
5095 903 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3070 3070
Size: 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2124 2206 2252 2518 2534 2616
Size: 399 223 111 82 46 266 16 82
Cuts at: 2616 2688 2751 2868 3034 3042 3160 3184 3410
Size: 72 63 117 166 8 118 24 226
Cuts at: 3410 4152 4237 5308 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 266 226 223 166
118 117 111 85 82 82 72 63
46 24 16 8
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 3446 844
Size: 1310 1292 3489
Fragments arranged by size:
3489 1310 1292
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 4586 4760 65
Size: 1183 1179 2159 174 1396
Fragments arranged by size:
2159 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2468 4735 4922 1700
Size: 110 658 2267 187 2869
Fragments arranged by size:
2869 2267 658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 2028 2382 4151 5163 2028
Size: 354 1769 1012 2956
Fragments arranged by size:
2956 1769 1012 354
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 794
Size: 1352 4739
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 3367 3367
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2171 2171
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2004 2252 2349 2762 3352 513
Size: 552 939 248 97 413 590 3252
Fragments arranged by size:
3252 939 590 552 413 248 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3607 4401 4421 3097
Size: 510 794 20 4767
Fragments arranged by size:
4767 794 510 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3371 5098 809
Size: 507 2055 1727 1802
Fragments arranged by size:
2055 1802 1727 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3538 4427
Size: 54 1425 50 275 18 225 1483 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1483 1425 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3378 4021 1353
Size: 2025 643 3423
Fragments arranged by size:
3423 2025 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2429 2467 2948 4154 4751 5538
Size: 393 575 222 38 481 1206 597 787
Cuts at: 5538 5806 1239
Size: 268 1524
Fragments arranged by size:
1524 1206 787 597 575 481 393 268
222 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2707
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 2009 2182 2203 2248 2839 2849 2887 3030 3031
Size: 173 21 45 591 10 38 143 1
Cuts at: 3031 3118 3157 3267 3275 3298 2009
Size: 87 39 110 8 23 4802
Fragments arranged by size:
4802 591 173 143 110 87 45 39
38 23 21 10 8 1
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2159 2937 3010 3190 3334 5238 1678
Size: 481 778 73 180 144 1904 2531
Fragments arranged by size:
2531 1904 778 481 180 144 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373
Size: 32 135 32 3160 32 42 32 2626
Fragments arranged by size:
3160 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 4044
Fragments arranged by size:
4044 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2703 4049 4408 4567 518
Size: 648 1537 1346 359 159 2042
Fragments arranged by size:
2042 1537 1346 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2254 2521 2785 2878 3153 3507 4184
Size: 573 75 267 264 93 275 354 677
Cuts at: 4184 5332 5577 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 573 354 275 267 264
245 93 75
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 4255
Fragments arranged by size:
4255 939 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2649 3449 4994 1132
Size: 97 1420 800 1545 2229
Fragments arranged by size:
2229 1545 1420 800 97
PasI CC'CwG_GG
Cuts at: 1348 3130 1348
Size: 1782 4309
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2070 2616 2070
Size: 546 5545
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2849 2887 3267 2182
Size: 667 38 380 5006
Fragments arranged by size:
5006 667 380 38
PshAI GACnn'nnGTC
Cuts at: 1613 2279 3222 1613
Size: 666 943 4482
Fragments arranged by size:
4482 943 666
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2009 2202 2248 2530 3030 3156 3180 2009
Size: 193 46 282 500 126 24 4920
Fragments arranged by size:
4920 500 282 193 126 46 24
PstI C_TGCA'G
Cuts at: 830 2587 2811 2933 3443 830
Size: 1757 224 122 510 3478
Fragments arranged by size:
3478 1757 510 224 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2521 2785 2521
Size: 264 5827
SacII CC_GC'GG
Cuts at: 2255 2255
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SanDI GG'GwC_CC
Cuts at: 2887 3267 2887
Size: 380 5711
SapI GCTCTTCn'nnn_
Cuts at: 3378 3378
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2583 2807 2929 3439 4574 5255 5446
Size: 219 1538 224 122 510 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1538 1471 1135 681 510 224 219 191
122
SfiI GGCCn_nnn'nGGCC
Cuts at: 3125 3125
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 4195 5096 5358 820
Size: 197 926 327 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 327 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 2740 2817 1517
Size: 1223 77 4791
Fragments arranged by size:
4791 1223 77
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 2741 3530 513
Size: 552 939 345 392 789 3074
Fragments arranged by size:
3074 939 789 552 392 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2883 3062 3340 4118 4135 4288
Size: 1255 531 772 179 278 778 17 153
Cuts at: 4288 4473 4918 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 778 772 531 445 278 185
179 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 4337 6049
Size: 274 80 33 51 1443 516 1844 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1844 1712 1443 516 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2480 4220 2480
Size: 1740 4351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SexAI SfoI SrfI SwaI