Restriction map for
pFN21AE1143
(Circular) (Six-base) MAPSORT of: pFN21AE1143.seq Check: 8361 from: 1 to: 5980
pFN21AE1143 5980 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2073 3790 278
Size: 53 83 186 936 537 1717 2468
Fragments arranged by size:
2468 1717 936 537 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 3323 1937
Size: 130 1256 4594
Fragments arranged by size:
4594 1256 130
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 2566 4107 4480 1563
Size: 1003 1541 373 3063
Fragments arranged by size:
3063 1541 1003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 2852 4043 5427 5695 1632
Size: 825 395 1191 1384 268 1917
Fragments arranged by size:
1917 1384 1191 825 395 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2523 1184
Size: 1339 4641
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2223 2290 2326 2365 3289 3515 3667 5776
Size: 730 67 36 39 924 226 152 2109
Cuts at: 5776 1493
Size: 1697
Fragments arranged by size:
2109 1697 924 730 226 152 67 39
36
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3305 1870
Size: 73 1362 4545
Fragments arranged by size:
4545 1362 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2507 3301 4756 618
Size: 325 217 627 234 486 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 486 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2052 2159 3299 1391
Size: 661 107 1140 4072
Fragments arranged by size:
4072 1140 661 107
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 5980
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 2487 3149 928
Size: 971 546 42 662 3759
Fragments arranged by size:
3759 971 662 546 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3073 3872 5082 3073
Size: 799 1210 3971
Fragments arranged by size:
3971 1210 799
BclI T'GATC_A
Cuts at: 1548 2662 1548
Size: 1114 4866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2977 5975 2977
Size: 2998 2982
BlpI GC'TnA_GC
Cuts at: 2883 3397 2883
Size: 514 5466
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2114 2795 4640 1239
Size: 827 48 681 1845 2579
Fragments arranged by size:
2579 1845 827 681 48
Bpu10I CC'TnA_GC
Cuts at: 3086 3086
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 3149 4105 4970 5268 2096
Size: 1053 956 865 298 2808
Fragments arranged by size:
2808 1053 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2413 4643 882
Size: 617 914 2230 2219
Fragments arranged by size:
2230 2219 914 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2070 3787 4169
Size: 53 83 186 936 489 48 1717 382
Cuts at: 4169 4804 275
Size: 635 1451
Fragments arranged by size:
1717 1451 936 635 489 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2102 1186
Size: 907 9 5064
Fragments arranged by size:
5064 907 9
BseYI C'CCAG_C
Cuts at: 2027 5183 5679 2027
Size: 3156 496 2328
Fragments arranged by size:
3156 2328 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2179 2577 1163
Size: 573 443 398 4566
Fragments arranged by size:
4566 573 443 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 4041 4126 5197 2144
Size: 1897 85 1071 2927
Fragments arranged by size:
2927 1897 1071 85
BsmI GAATG_Cn'
Cuts at: 2339 3491 3584 2339
Size: 1152 93 4735
Fragments arranged by size:
4735 1152 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2144 2159 3299 4041 4126 5197
Size: 399 262 92 15 1140 742 85 1071
Cuts at: 5197 1391
Size: 2174
Fragments arranged by size:
2174 1140 1071 742 399 262 92 85
15
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2201 2957 3335 844
Size: 1357 756 378 3489
Fragments arranged by size:
3489 1357 756 378
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2786 4624 4811 1700
Size: 110 976 1838 187 2869
Fragments arranged by size:
2869 1838 976 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2180 794
Size: 1386 4594
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2977 3310 3677 4062 4079 5531 5617 5629
Size: 1005 333 367 385 17 1452 86 12
Cuts at: 5629 5975 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 333 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2491 513
Size: 552 939 48 439 4002
Fragments arranged by size:
4002 939 552 439 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3496 4290 4310 2904
Size: 592 794 20 4574
Fragments arranged by size:
4574 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 2323 3284 2323
Size: 961 5019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316
Size: 54 1425 50 275 18 177 1420 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3219 3244 3910 1353
Size: 1866 25 666 3423
Fragments arranged by size:
3423 1866 666 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2114 2457 2795 2852 4043 4640
Size: 393 434 48 343 338 57 1191 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1191 787 597 434 393 343 338
268 57 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2714 2078
Size: 636 5344
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2025 5127 1678
Size: 347 3102 2531
Fragments arranged by size:
3102 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 3218 3250 4621
Size: 32 135 32 838 32 776 32 1371
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1371 838 776 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 3324 3570 669
Size: 988 281 130 1256 246 3079
Fragments arranged by size:
3079 1256 988 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 2597 3579 2597
Size: 982 4998
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2019 5094 5278 1305
Size: 714 3075 184 2007
Fragments arranged by size:
3075 2007 714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 3938 4297 4456 518
Size: 648 1339 1433 359 159 2042
Fragments arranged by size:
2042 1433 1339 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 3396 4073 5221 5466 1606
Size: 421 1369 677 1148 245 2120
Fragments arranged by size:
2120 1369 1148 677 421 245
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 5980
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 2491 513
Size: 552 939 487 4002
Fragments arranged by size:
4002 939 552 487
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2916 3112 1108
Size: 1808 196 3976
Fragments arranged by size:
3976 1808 196
PfoI T'CCnGG_A
Cuts at: 2748 2748
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 2894 3550 2894
Size: 656 5324
PstI C_TGCA'G
Cuts at: 830 3278 3332 830
Size: 2448 54 3478
Fragments arranged by size:
3478 2448 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 2066 3322 1936
Size: 130 1256 4594
Fragments arranged by size:
4594 1256 130
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2185 3274 3328 4463 5144 5335 826
Size: 219 1140 1089 54 1135 681 191 1471
Fragments arranged by size:
1471 1140 1135 1089 681 219 191 54
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 5980
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 3164 4084 4985 5247 820
Size: 197 926 132 1089 920 901 262 1553
Fragments arranged by size:
1553 1089 926 920 901 262 197 132
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 2555 1517
Size: 1038 4942
StyI C'CwwG_G
Cuts at: 513 1065 2004 2491 3419 513
Size: 552 939 487 928 3074
Fragments arranged by size:
3074 939 928 552 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 2403 4226
Size: 274 80 33 51 1443 402 24 1823
Cuts at: 4226 5938 96
Size: 1712 138
Fragments arranged by size:
1823 1712 1443 402 274 138 80 51
33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 2980 3316 2980
Size: 336 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 3228 4109 3228
Size: 881 5099
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2071 3788 276
Size: 53 83 186 936 537 1717 2468
Fragments arranged by size:
2468 1717 936 537 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI