Restriction map for
pFN21AE1138
(Circular) (Six-base) MAPSORT of: pFN21AE1138.seq Check: 568 from: 1 to: 5821
pFN21AE1138 5821 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 3884 5268 5536 1632
Size: 807 1445 1384 268 1917
Fragments arranged by size:
1917 1445 1384 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 2095 3564 5136 2095
Size: 1469 1572 2780
Fragments arranged by size:
2780 1572 1469
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2831 3130 3356 3508 5617 1493
Size: 1338 299 226 152 2109 1697
Fragments arranged by size:
2109 1697 1338 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 3142 4597 618
Size: 325 217 627 578 777 1455 1842
Fragments arranged by size:
1842 1455 777 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 3140 1391
Size: 805 944 4072
Fragments arranged by size:
4072 944 805
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2167 3882 5038 1790
Size: 377 1715 1156 2573
Fragments arranged by size:
2573 1715 1156 377
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2439 3552 4510
Size: 416 53 83 399 24 1022 1113 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1113 1022 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 1239
Size: 3242 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2541 3628 4010
Size: 53 83 186 936 476 532 1087 382
Cuts at: 4010 4645 275
Size: 635 1451
Fragments arranged by size:
1451 1087 936 635 532 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2179 2743 4254 4652 4926 5073 5484 2179
Size: 564 1511 398 274 147 411 2516
Fragments arranged by size:
2516 1511 564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2629 2659 2888 2918 1178
Size: 30 165 30 1226 30 229 30 4081
Fragments arranged by size:
4081 1226 229 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2507 2510 2519 2522 2909 1186
Size: 1318 3 3 9 3 387 4098
Fragments arranged by size:
4098 1318 387 9 3 3 3
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2196 2580 3882 3967 5038 2102
Size: 94 384 1302 85 1071 2885
Fragments arranged by size:
2885 1302 1071 384 94 85
BsmI GAATG_Cn'
Cuts at: 3052 3332 3425 3052
Size: 280 93 5448
Fragments arranged by size:
5448 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2167 2196 2580 3140 3882 3967
Size: 399 312 65 29 384 560 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 560 399 384 312 85
65 29
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2842 3176 844
Size: 1998 334 3489
Fragments arranged by size:
3489 1998 334
BsrBI CCG'CTC
Cuts at: 1394 1823 2554 3708 1394
Size: 429 731 1154 3507
Fragments arranged by size:
3507 1154 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2370 2766 2370
Size: 396 5425
BtgI C'CryG_G
Cuts at: 513 1065 2004 2730 513
Size: 552 939 726 3604
Fragments arranged by size:
3604 939 726 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3072 487
Size: 683 56 1846 3236
Fragments arranged by size:
3236 1846 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 2928 1554
Size: 1374 4447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2389 2424 529
Size: 35 1825 35 3926
Fragments arranged by size:
3926 1825 35 35
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2339 4828 809
Size: 507 1023 2489 1802
Fragments arranged by size:
2489 1802 1023 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 3268 4157
Size: 54 1425 50 275 18 411 1027 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1027 889 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2596 2880 3036 3751 1353
Size: 1143 100 284 156 715 3423
Fragments arranged by size:
3423 1143 715 284 156 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2005 2011 2272 4408 4782 4928
Size: 1064 138 416 6 261 2136 374 146
Cuts at: 4928 387
Size: 1280
Fragments arranged by size:
2136 1280 1064 416 374 261 146 138
6
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 3884 4481 5268 5536 1239
Size: 393 807 1445 597 787 268 1524
Fragments arranged by size:
1524 1445 807 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2670 2670
Size: 5821
EcoO109I rG'GnC_Cy
Cuts at: 2653 2653
Size: 5821
EcoRI G'AATT_C
Cuts at: 2831 3130 3508 2831
Size: 299 378 5144
Fragments arranged by size:
5144 378 299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 5821
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2297 2321 4968 1678
Size: 619 24 2647 2531
Fragments arranged by size:
2647 2531 619 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2107 2121 2139 3071
Size: 32 135 32 517 18 14 18 932
Cuts at: 3071 3103 4462 4494 4536 4568 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 932 517 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3165 3411 669
Size: 988 281 919 308 246 3079
Fragments arranged by size:
3079 988 919 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 2679 3420 2679
Size: 741 5080
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2087 2583 2886 4935 5119 1305
Size: 782 496 303 2049 184 2007
Fragments arranged by size:
2049 2007 782 496 303 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2392 3779 4138 4297 518
Size: 648 1197 29 1387 359 159 2042
Fragments arranged by size:
2042 1387 1197 648 359 159 29
MspA1I CmG'CkG
Cuts at: 1606 2155 2732 3237 3914 5062 5307 1606
Size: 549 577 505 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 549 505 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NruI TCG'CGA
Cuts at: 2132 2132
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2210 1613
Size: 597 5224
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3030 3173 830
Size: 2200 143 3478
Fragments arranged by size:
3478 2200 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5821
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 5821
SacII CC_GC'GG
Cuts at: 2733 2733
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 2638 4069 5781 2638
Size: 1431 1712 2678
Fragments arranged by size:
2678 1712 1431
SfcI C'TryA_G
Cuts at: 826 1045 3026 3074 3169 4304 4985 5176 826
Size: 219 1981 48 95 1135 681 191 1471
Fragments arranged by size:
1981 1471 1135 681 219 191 95 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2649 3260 513
Size: 552 939 357 288 611 3074
Fragments arranged by size:
3074 939 611 552 357 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2636 4067
Size: 274 80 33 51 1443 159 500 1431
Cuts at: 4067 5779 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1431 500 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 2807 2839 5781 5813 2570
Size: 32 205 32 2942 32 2578
Fragments arranged by size:
2942 2578 205 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI