Restriction map for
pFN21AE1135
(Circular) (Six-base) MAPSORT of: pFN21AE1135.seq Check: 7506 from: 1 to: 5764
pFN21AE1135 5764 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3024 3107 1937
Size: 1087 83 4594
Fragments arranged by size:
4594 1087 83
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 3827 5211 5479 1632
Size: 466 1729 1384 268 1917
Fragments arranged by size:
1917 1729 1384 466 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2485 4663 5640 1203
Size: 1282 2178 977 1327
Fragments arranged by size:
2178 1327 1282 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 2466 4493 2466
Size: 2027 3737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 2450 2569 2779 2819 2854 3507 5079 2450
Size: 119 210 40 35 653 1572 3135
Fragments arranged by size:
3135 1572 653 210 119 40 35
ApaI G_GGCC'C
Cuts at: 2047 2150 3053 2047
Size: 103 903 4758
Fragments arranged by size:
4758 903 103
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 3073 3299 3451 5560 1493
Size: 1580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2124 3030 3089 1870
Size: 73 126 55 906 59 4545
Fragments arranged by size:
4545 906 126 73 59 55
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2164 2672 2740 2867
Size: 325 217 627 286 91 508 68 127
Cuts at: 2867 2878 2949 2955 2972 3085 4540 618
Size: 11 71 6 17 113 1455 1842
Fragments arranged by size:
1842 1455 627 508 325 286 217 127
113 91 71 68 17 11 6
BanII G_rGCy'C
Cuts at: 1391 2047 2150 2843 3053 3083 1391
Size: 656 103 693 210 30 4072
Fragments arranged by size:
4072 693 656 210 103 30
BbeI G_GCGC'C
Cuts at: 2744 2959 2744
Size: 215 5549
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4793
BbvCI CC'TCA_GC
Cuts at: 2348 2820 2348
Size: 472 5292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3955 3989 1903
Size: 34 50 34 1934 34 3678
Fragments arranged by size:
3678 1934 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 4375 136
Size: 107 122 71 631 605 686 2017 1525
Fragments arranged by size:
2017 1525 686 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2150 2169 2482 2526 2604 2870 2883
Size: 257 103 19 313 44 78 266 13
Cuts at: 2883 2975 3053 3825 4981 1790
Size: 92 78 772 1156 2573
Fragments arranged by size:
2573 1156 772 313 266 257 103 92
78 78 44 19 13
BmgBI CAC'GTC
Cuts at: 1318 2410 1318
Size: 1092 4672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2739 2818 3495
Size: 416 53 83 399 24 1322 79 677
Cuts at: 3495 4453 442
Size: 958 1753
Fragments arranged by size:
1753 1322 958 677 416 399 83 79
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2354 2980 4424 1239
Size: 995 120 626 1444 2579
Fragments arranged by size:
2579 1444 995 626 120
Bpu10I CC'TnA_GC
Cuts at: 2348 2402 2417 2633 2733 2820 2837 2348
Size: 54 15 216 100 87 17 5275
Fragments arranged by size:
5275 216 100 87 54 17 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 2446 4427 882
Size: 617 585 362 1981 2219
Fragments arranged by size:
2219 1981 617 585 362
BsaAI yAC'GTr
Cuts at: 493 1204 2488 3555 493
Size: 711 1284 1067 2702
Fragments arranged by size:
2702 1284 1067 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2741 2956 3571 3953
Size: 53 83 186 936 1208 215 615 382
Cuts at: 3953 4588 275
Size: 635 1451
Fragments arranged by size:
1451 1208 936 635 615 382 215 186
83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 2808 2838 1178
Size: 30 165 30 783 30 592 30 4104
Fragments arranged by size:
4104 783 592 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2891 1186
Size: 1705 4059
BseYI C'CCAG_C
Cuts at: 2686 4967 5463 2686
Size: 2281 496 2987
Fragments arranged by size:
2987 2281 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2999 1163
Size: 573 1263 3928
Fragments arranged by size:
3928 1263 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3825 3910 4981 2843
Size: 982 85 1071 3626
Fragments arranged by size:
3626 1071 982 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2150 2169 2482 2526 2604 2843
Size: 399 257 103 19 313 44 78 239
Cuts at: 2843 2870 2883 2975 3053 3083 3825 3910 4981
Size: 27 13 92 78 30 742 85 1071
Cuts at: 4981 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 313 257 239 103
92 85 78 78 44 30 27 19
13
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 2764 3119 844
Size: 1451 469 355 3489
Fragments arranged by size:
3489 1451 469 355
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 2208 2540 2586 2952 4408 4595
Size: 110 351 47 332 46 366 1456 187
Cuts at: 4595 1700
Size: 2869
Fragments arranged by size:
2869 1456 366 351 332 187 110 47
46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2519 2533 3824 4836 2519
Size: 14 1291 1012 3447
Fragments arranged by size:
3447 1291 1012 14
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2212 3061 2176
Size: 36 849 4879
Fragments arranged by size:
4879 849 36
BstYI r'GATC_y
Cuts at: 1972 2272 3094 3461 3846 3863 5315 5401 5413
Size: 300 822 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 822 385 367 346 300 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2889 2889
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2009 513
Size: 552 944 4268
Fragments arranged by size:
4268 944 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2915 3016 3280 4074 4094 2307
Size: 608 101 264 794 20 3977
Fragments arranged by size:
3977 794 608 264 101 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2057 2712
Size: 54 1425 50 275 18 201 26 655
Cuts at: 2712 3211 4100 5729 8
Size: 499 889 1629 43
Fragments arranged by size:
1629 1425 889 655 499 275 201 54
50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 2841
Size: 5764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2234 2354 2980 3827 4424 5211
Size: 393 466 136 120 626 847 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 847 787 626 597 466 393 268
136 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2888 2888
Size: 5764
EcoO109I rG'GnC_Cy
Cuts at: 2043 2147 2776 3049 3050 2043
Size: 104 629 273 1 4757
Fragments arranged by size:
4757 629 273 104 1
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FseI GG_CCGG'CC
Cuts at: 2544 2544
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2744 2897 2959 4911 1678
Size: 1066 153 62 1952 2531
Fragments arranged by size:
2531 1952 1066 153 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2452 2484 4405
Size: 32 135 32 614 32 234 32 1921
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1921 614 234 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2801 748
Size: 2053 3711
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KasI G'GCGC_C
Cuts at: 2740 2955 2740
Size: 215 5549
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2229 4878 5062 1305
Size: 924 2649 184 2007
Fragments arranged by size:
2649 2007 924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 10
Size: 54 1425 343 882 3060
Fragments arranged by size:
3060 1425 882 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3722 4081 4240 518
Size: 648 996 1560 359 159 2042
Fragments arranged by size:
2042 1560 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3180 3857 5005 5250 1606
Size: 1574 677 1148 245 2120
Fragments arranged by size:
2120 1574 1148 677 245
NaeI GCC'GGC
Cuts at: 2542 2954 2542
Size: 412 5352
NarI GG'CG_CC
Cuts at: 2741 2956 2741
Size: 215 5549
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5212
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NgoMIV G'CCGG_C
Cuts at: 2540 2952 2540
Size: 412 5352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4862
PfoI T'CCnGG_A
Cuts at: 2433 2778 2433
Size: 345 5419
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PmlI CAC'GTG
Cuts at: 2488 2488
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 2776
Size: 5764
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2043 2146 3049 2043
Size: 103 903 4758
Fragments arranged by size:
4758 903 103
PstI C_TGCA'G
Cuts at: 830 2724 2775 2850 2922 3116 830
Size: 1894 51 75 72 194 3478
Fragments arranged by size:
3478 1894 194 75 72 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
SacI G_AGCT'C
Cuts at: 2843 2843
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 2775 2850 3116 2775
Size: 75 266 5423
Fragments arranged by size:
5423 266 75
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2874 2874
Size: 5764
SfcI C'TryA_G
Cuts at: 826 1045 2720 2771 2846 2918 3112 4247 4928
Size: 219 1675 51 75 72 194 1135 681
Cuts at: 4928 5119 826
Size: 191 1471
Fragments arranged by size:
1675 1471 1135 681 219 194 191 75
72 51
SfoI GGC'GCC
Cuts at: 2742 2957 2742
Size: 215 5549
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2071 3032 3091 1872
Size: 199 961 59 4545
Fragments arranged by size:
4545 961 199 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2694 2835 2989 1517
Size: 1177 141 154 4292
Fragments arranged by size:
4292 1177 154 141
StyI C'CwwG_G
Cuts at: 513 1065 3003 3203 513
Size: 552 1938 200 3074
Fragments arranged by size:
3074 1938 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 3791 3808 3961 4146 4591 325
Size: 1255 740 1471 17 153 185 445 1498
Fragments arranged by size:
1498 1471 1255 740 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 2933 2965 5724 5756 2808
Size: 32 93 32 2759 32 2816
Fragments arranged by size:
2816 2759 93 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3004 3004
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 2069 3030 3089 1870
Size: 199 961 59 4545
Fragments arranged by size:
4545 961 199 59
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI NruI
NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfiI SrfI SwaI