Restriction map for
pFN21AE1131
(Circular) (Six-base) MAPSORT of: pFN21AE1131.seq Check: 5608 from: 1 to: 5482
pFN21AE1131 5482 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2228 2669 3545 4929 5197 1632
Size: 530 66 441 876 1384 268 1917
Fragments arranged by size:
1917 1384 876 530 441 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 2127 4381 5358 1203
Size: 924 2254 977 1327
Fragments arranged by size:
2254 1327 977 924
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 2140 2272 2402 2605 2638 3225 4797 2140
Size: 132 130 203 33 587 1572 2825
Fragments arranged by size:
2825 1572 587 203 132 130 33
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2791 3017 3169 5278 1493
Size: 1298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 1870
Size: 73 864 4545
Fragments arranged by size:
4545 864 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2094 2803 4258 618
Size: 325 217 627 307 709 1455 1842
Fragments arranged by size:
1842 1455 709 627 325 307 217
BanII G_rGCy'C
Cuts at: 1391 2119 2801 1391
Size: 728 682 4072
Fragments arranged by size:
4072 728 682
BbeI G_GCGC'C
Cuts at: 2098 2098
Size: 5482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2657 928
Size: 971 758 3753
Fragments arranged by size:
3753 971 758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 2175 1548
Size: 627 4855
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4093 136
Size: 107 122 71 631 605 337 2084 1525
Fragments arranged by size:
2084 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2472 2899 2472
Size: 427 5055
Bme1580I G_kGCm'C
Cuts at: 1790 2316 3543 4699 1790
Size: 526 1227 1156 2573
Fragments arranged by size:
2573 1227 1156 526
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3213 4171 442
Size: 416 53 83 399 24 1796 958 1753
Fragments arranged by size:
1796 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2120 2448 2492 2660 2690 4142 1239
Size: 803 78 328 44 168 30 1452 2579
Fragments arranged by size:
2579 1452 803 328 168 78 44 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 3607 4472 4770 2537
Size: 1070 865 298 3249
Fragments arranged by size:
3249 1070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 3273 493
Size: 711 906 1163 2702
Fragments arranged by size:
2702 1163 906 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2095 3289 3671
Size: 53 83 186 936 479 83 1194 382
Cuts at: 3671 4306 275
Size: 635 1451
Fragments arranged by size:
1451 1194 936 635 479 382 186 83
83 53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 2233 1186
Size: 1044 3 4435
Fragments arranged by size:
4435 1044 3
BseYI C'CCAG_C
Cuts at: 2442 4685 5181 2442
Size: 2243 496 2743
Fragments arranged by size:
2743 2243 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3543 3628 4699 3543
Size: 85 1071 4326
Fragments arranged by size:
4326 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2316 2801 3543 3628 4699 1391
Size: 399 329 197 485 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 329 197 85
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 4126 4313 1700
Size: 110 351 1965 187 2869
Fragments arranged by size:
2869 1965 351 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 2761 4286 2761
Size: 1525 3957
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2566
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2075 487
Size: 683 56 849 3894
Fragments arranged by size:
3894 849 683 56
BtsI GCAGTG_nn'
Cuts at: 2998 3792 3812 2998
Size: 794 20 4668
Fragments arranged by size:
4668 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2281 2568
Size: 54 1425 50 275 18 429 22 287
Cuts at: 2568 2929 3818 5447 8
Size: 361 889 1629 43
Fragments arranged by size:
1629 1425 889 429 361 287 275 54
50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 2520 2759 3412 1353
Size: 1061 106 239 653 3423
Fragments arranged by size:
3423 1061 653 239 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 2642 4069 4443 4589 387
Size: 1064 138 948 105 1427 374 146 1280
Fragments arranged by size:
1427 1280 1064 948 374 146 138 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2120 2162 2228 2448 2492 2660
Size: 393 410 78 42 66 220 44 168
Cuts at: 2660 2669 2690 3545 4142 4929 5197 1239
Size: 9 21 855 597 787 268 1524
Fragments arranged by size:
1524 855 787 597 410 393 268 220
168 78 66 44 42 21 9
EcoO109I rG'GnC_Cy
Cuts at: 2080 2137 2439 2080
Size: 57 302 5123
Fragments arranged by size:
5123 302 57
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2098 4629 1678
Size: 420 2531 2531
Fragments arranged by size:
2531 2531 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2317 2349 2447 2479 4123
Size: 32 135 32 745 32 98 32 1644
Cuts at: 4123 4155 4197 4229 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1644 745 135 98 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2695 2826 3072 669
Size: 988 281 757 131 246 3079
Fragments arranged by size:
3079 988 757 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KasI G'GCGC_C
Cuts at: 2094 2094
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4596 4780 1305
Size: 3291 184 2007
Fragments arranged by size:
3291 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 10
Size: 54 1425 343 429 3231
Fragments arranged by size:
3231 1425 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3440 3799 3958 518
Size: 648 1578 696 359 159 2042
Fragments arranged by size:
2042 1578 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2069 2471 2898 3575 4723 4968 1606
Size: 446 17 402 427 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 446 427 402 245 17
NarI GG'CG_CC
Cuts at: 2095 2095
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2131 2840 4385 1132
Size: 97 902 709 1545 2229
Fragments arranged by size:
2229 1545 902 709 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5482
PciI A'CATG_T
Cuts at: 2127 4381 2127
Size: 2254 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 2638 1108
Size: 1164 366 3952
Fragments arranged by size:
3952 1164 366
PfoI T'CCnGG_A
Cuts at: 2189 2508 2189
Size: 319 5163
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2439 2080
Size: 359 5123
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2340 2398 2493 2834 830
Size: 1510 58 95 341 3478
Fragments arranged by size:
3478 1510 341 95 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2471 2471
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SanDI GG'GwC_CC
Cuts at: 2080 2439 2080
Size: 359 5123
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SexAI A'CCwGG_T
Cuts at: 2031 2031
Size: 5482
SfcI C'TryA_G
Cuts at: 826 1045 2336 2394 2489 2830 3965 4646 4837
Size: 219 1291 58 95 341 1135 681 191
Cuts at: 4837 826
Size: 1471
Fragments arranged by size:
1471 1291 1135 681 341 219 191 95
58
SfoI GGC'GCC
Cuts at: 2096 2096
Size: 5482
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3586 4487 4749 820
Size: 197 926 609 1034 901 262 1553
Fragments arranged by size:
1553 1034 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 2186 3406 5
Size: 47 2134 1220 2081
Fragments arranged by size:
2134 2081 1220 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3074
Fragments arranged by size:
3074 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 2605 2656 1316
Size: 1167 122 51 4142
Fragments arranged by size:
4142 1167 122 51
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2269
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SfiI SrfI SwaI