Restriction map for
pFN21AE1128
(Circular) (Six-base) MAPSORT of: pFN21AE1128.seq Check: 274 from: 1 to: 5200
pFN21AE1128 5200 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2153 2153
Size: 5200
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3010 278
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 1937
Size: 606 4594
Acc65I G'GTAC_C
Cuts at: 2521 2521
Size: 5200
AclI AA'CG_TT
Cuts at: 1563 3327 3700 1563
Size: 1764 373 3063
Fragments arranged by size:
3063 1764 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3263 4647 4915 1632
Size: 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5003
AflIII A'CryG_T
Cuts at: 1203 4099 5076 1203
Size: 2896 977 1327
Fragments arranged by size:
2896 1327 977
AgeI A'CCGG_T
Cuts at: 4031 4031
Size: 5200
AhdI GACnn_n'nnGTC
Cuts at: 3929 3929
Size: 5200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5200
AlwNI CAG_nnn'CTG
Cuts at: 2943 4515 2943
Size: 1572 3628
ApaI G_GGCC'C
Cuts at: 2135 2135
Size: 5200
ApaLI G'TGCA_C
Cuts at: 3257 4413 3257
Size: 1156 4044
ApoI r'AATT_y
Cuts at: 1493 2509 2735 2887 4996 1493
Size: 1016 226 152 2109 1697
Fragments arranged by size:
2109 1697 1016 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3754 160
Size: 872 2722 1606
Fragments arranged by size:
2722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 1870
Size: 73 582 4545
Fragments arranged by size:
4545 582 73
BamHI G'GATC_C
Cuts at: 2530 2897 2530
Size: 367 4833
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 3976 618
Size: 325 217 627 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2394 2519 1391
Size: 744 259 125 4072
Fragments arranged by size:
4072 744 259 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2145 928
Size: 971 246 3983
Fragments arranged by size:
3983 971 246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3391 3425 1903
Size: 34 1454 34 3678
Fragments arranged by size:
3678 1454 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3092 4302 3092
Size: 1210 3990
BclI T'GATC_A
Cuts at: 1548 2124 1548
Size: 576 4624
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3811 136
Size: 107 122 71 631 605 2139 1525
Fragments arranged by size:
2139 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5195 5195
Size: 5200
BlpI GC'TnA_GC
Cuts at: 2617 2617
Size: 5200
Bme1580I G_kGCm'C
Cuts at: 1790 2135 3261 4417 1790
Size: 345 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 345
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3889 442
Size: 416 53 83 399 24 1514 958 1753
Fragments arranged by size:
1753 1514 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3860 1239
Size: 2621 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 3325 4190 4488 2006
Size: 1319 865 298 2718
Fragments arranged by size:
2718 1319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3863 882
Size: 617 2364 2219
Fragments arranged by size:
2364 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2991 493
Size: 711 1787 2702
Fragments arranged by size:
2702 1787 711
BsaBI GATnn'nnATC
Cuts at: 5194 5194
Size: 5200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3007 3389 4024 275
Size: 53 83 186 936 1474 382 635 1451
Fragments arranged by size:
1474 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2065 3633 4031 4305 4452 4863 2065
Size: 1568 398 274 147 411 2402
Fragments arranged by size:
2402 1568 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 1178
Size: 30 165 30 997 30 3948
Fragments arranged by size:
3948 997 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5200
BseYI C'CCAG_C
Cuts at: 4403 4899 4403
Size: 496 4704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 1163
Size: 573 627 4000
Fragments arranged by size:
4000 627 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2650 3411 4439 1540
Size: 275 186 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 186
BsiHKAI G_wGCw'C
Cuts at: 3261 3346 4417 3261
Size: 85 1071 4044
Fragments arranged by size:
4044 1071 85
BsmI GAATG_Cn'
Cuts at: 2711 2804 2711
Size: 93 5107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2394 2519 3261 3346 4417 1391
Size: 399 345 259 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 259 125 85
BspEI T'CCGG_A
Cuts at: 2065 4863 2065
Size: 2798 2402
BspHI T'CATG_A
Cuts at: 3089 4819 3089
Size: 1730 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2153 2555 844
Size: 1309 402 3489
Fragments arranged by size:
3489 1309 402
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 3087 1394
Size: 429 506 758 3507
Fragments arranged by size:
3507 758 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3695 3869 65
Size: 1183 2447 174 1396
Fragments arranged by size:
2447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3844 4031 1700
Size: 110 2034 187 2869
Fragments arranged by size:
2869 2034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5200
BssSI C'ACGA_G
Cuts at: 3260 4272 3260
Size: 1012 4188
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5200
BstBI TT'CG_AA
Cuts at: 4004 4004
Size: 5200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5200
BstYI r'GATC_y
Cuts at: 1972 2358 2530 2897 3282 3299 4751 4837 4849
Size: 386 172 367 385 17 1452 86 12
Cuts at: 4849 5195 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 386 385 367 346 172 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2304 513
Size: 552 939 300 3409
Fragments arranged by size:
3409 939 552 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2310 2454 487
Size: 683 56 1084 144 3233
Fragments arranged by size:
3233 1084 683 144 56
BtsI GCAGTG_nn'
Cuts at: 2716 3510 3530 2716
Size: 794 20 4386
Fragments arranged by size:
4386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5165
DraI TTT'AAA
Cuts at: 2504 2504
Size: 5200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4207 809
Size: 507 2891 1802
Fragments arranged by size:
2891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2647 3536
Size: 54 1425 50 275 18 204 613 889
Cuts at: 3536 5165 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 613 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2647 1537
Size: 275 835 4090
Fragments arranged by size:
4090 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 3130 1353
Size: 738 1039 3423
Fragments arranged by size:
3423 1039 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3787 4161 4307 387
Size: 1064 138 2198 374 146 1280
Fragments arranged by size:
2198 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3263 3860 4647 4915 1239
Size: 393 1631 597 787 268 1524
Fragments arranged by size:
1631 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2067 2067
Size: 5200
EcoO109I rG'GnC_Cy
Cuts at: 2084 2084
Size: 5200
EcoRI G'AATT_C
Cuts at: 2509 2887 2509
Size: 378 4822
FspI TGC'GCA
Cuts at: 3706 3706
Size: 5200
HaeII r_GCGC'y
Cuts at: 1678 4347 1678
Size: 2669 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 3841 3873 3915
Size: 32 135 32 652 32 1585 32 42
Cuts at: 3915 3947 1373
Size: 32 2626
Fragments arranged by size:
2626 1585 652 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2544 2790 669
Size: 988 281 606 246 3079
Fragments arranged by size:
3079 988 606 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5200
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 5200
KpnI G_GTAC'C
Cuts at: 2525 2525
Size: 5200
MfeI C'AATT_G
Cuts at: 2799 2799
Size: 5200
MluI A'CGCG_T
Cuts at: 5076 5076
Size: 5200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4314 4498 1305
Size: 3009 184 2007
Fragments arranged by size:
3009 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3378
Fragments arranged by size:
3378 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3158 3517 3676 518
Size: 648 1992 359 159 2042
Fragments arranged by size:
2042 1992 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2616 3293 4441 4686 1606
Size: 1010 677 1148 245 2120
Fragments arranged by size:
2120 1148 1010 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2304 513
Size: 552 939 300 3409
Fragments arranged by size:
3409 939 552 300
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5200
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5200
NotI GC'GGCC_GC
Cuts at: 2647 2647
Size: 5200
NspI r_CATG'y
Cuts at: 1132 1229 2383 2558 4103 1132
Size: 97 1154 175 1545 2229
Fragments arranged by size:
2229 1545 1154 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5200
PciI A'CATG_T
Cuts at: 4099 4099
Size: 5200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2433 1108
Size: 1325 3875
PmeI GTTT'AAAC
Cuts at: 2504 2504
Size: 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4782 4814 3269
Size: 32 1481 32 3655
Fragments arranged by size:
3655 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5200
PsiI TTA'TAA
Cuts at: 2770 2770
Size: 5200
PspOMI G'GGCC_C
Cuts at: 2131 2131
Size: 5200
PstI C_TGCA'G
Cuts at: 830 2552 830
Size: 1722 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5200
SalI G'TCGA_C
Cuts at: 1936 2542 1936
Size: 606 4594
SbfI CC_TGCA'GG
Cuts at: 2552 2552
Size: 5200
ScaI AGT'ACT
Cuts at: 3448 5160 3448
Size: 1712 3488
SfcI C'TryA_G
Cuts at: 826 1045 2548 3683 4364 4555 826
Size: 219 1503 1135 681 191 1471
Fragments arranged by size:
1503 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5200
SmaI CCC'GGG
Cuts at: 1872 2527 1872
Size: 655 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 3304 4205 4467 820
Size: 197 926 78 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 2991 493
Size: 2498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5200
SphI G_CATG'C
Cuts at: 1132 2383 2558 1132
Size: 1251 175 3774
Fragments arranged by size:
3774 1251 175
SspI AAT'ATT
Cuts at: 5 52 3124 5
Size: 47 3072 2081
Fragments arranged by size:
3072 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5200
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 2639 513
Size: 552 939 300 335 3074
Fragments arranged by size:
3074 939 552 335 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3227 3244 3397 3582 4027 325
Size: 1255 1647 17 153 185 445 1498
Fragments arranged by size:
1647 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 3446 5158
Size: 274 80 33 51 1443 63 1406 1712
Cuts at: 5158 96
Size: 138
Fragments arranged by size:
1712 1443 1406 274 138 80 63 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2400 2432 5160 5192 2400
Size: 32 2728 32 2408
Fragments arranged by size:
2728 2408 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5200
XbaI T'CTAG_A
Cuts at: 2536 2536
Size: 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5200
XmaI C'CCGG_G
Cuts at: 1870 2525 1870
Size: 655 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 3329
Size: 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3008 276
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI