Restriction map for
pFN21AE1126
(Circular) (Six-base) MAPSORT of: pFN21AE1126.seq Check: 1400 from: 1 to: 6598
pFN21AE1126 6598 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2637 2637
Size: 6598
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3941 1937
Size: 2004 4594
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 2501 2558 2873 4661 6045 6313 1632
Size: 598 271 57 315 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 598 315 271 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 2390 5497 6474 1203
Size: 1187 3107 977 1327
Fragments arranged by size:
3107 1327 1187 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 6566
AlwNI CAG_nnn'CTG
Cuts at: 2851 2941 3046 3722 3740 4341 5913 2851
Size: 90 105 676 18 601 1572 3536
Fragments arranged by size:
3536 1572 676 601 105 90 18
ApaI G_GGCC'C
Cuts at: 2142 3333 2142
Size: 1191 5407
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 3907 4133 4285 6394 1493
Size: 2414 226 152 2109 1697
Fragments arranged by size:
2414 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3238 3238
Size: 6598
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2263 3923 1870
Size: 73 214 106 1660 4545
Fragments arranged by size:
4545 1660 214 106 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2654 2926 3919 5374
Size: 325 217 627 365 502 272 993 1455
Cuts at: 5374 618
Size: 1842
Fragments arranged by size:
1842 1455 993 627 502 365 325 272
217
BanII G_rGCy'C
Cuts at: 1391 2142 2166 2765 2822 3067 3333 3678 3882
Size: 751 24 599 57 245 266 345 204
Cuts at: 3882 3917 1391
Size: 35 4072
Fragments arranged by size:
4072 751 599 345 266 245 204 57
35 24
BbeI G_GCGC'C
Cuts at: 2156 2658 2156
Size: 502 6096
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5627
BbvCI CC'TCA_GC
Cuts at: 2834 2834
Size: 6598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3051 3085 4789 4823 1903
Size: 34 50 34 1030 34 1704 34 3678
Fragments arranged by size:
3678 1704 1030 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2851 4490 5700 2851
Size: 1639 1210 3749
Fragments arranged by size:
3749 1639 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2362 3518 3770
Size: 107 122 71 631 605 690 1156 252
Cuts at: 3770 3779 5209 136
Size: 9 1430 1525
Fragments arranged by size:
1525 1430 1156 690 631 605 252 122
107 71 9
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 2771 2816 3363 3582 4015 2771
Size: 45 547 219 433 5354
Fragments arranged by size:
5354 547 433 219 45
Bme1580I G_kGCm'C
Cuts at: 1790 2142 3333 4659 5815 1790
Size: 352 1191 1326 1156 2573
Fragments arranged by size:
2573 1326 1191 1156 352
BmgBI CAC'GTC
Cuts at: 1318 2391 1318
Size: 1073 5525
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2501 4329 5287
Size: 416 53 83 399 24 1084 1828 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
1828 1753 1084 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3287 1056
Size: 2231 4367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2612 2897 3777 3872 5258 1239
Size: 968 405 285 880 95 1386 2579
Fragments arranged by size:
2579 1386 968 880 405 285 95
Bpu10I CC'TnA_GC
Cuts at: 2766 2834 3288 2766
Size: 68 454 6076
Fragments arranged by size:
6076 454 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4723 5588 5886 4723
Size: 865 298 5435
Fragments arranged by size:
5435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5261 882
Size: 617 3762 2219
Fragments arranged by size:
3762 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2893 4389 493
Size: 711 1689 1496 2702
Fragments arranged by size:
2702 1689 1496 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 2655 3262 4405
Size: 53 83 186 936 620 502 607 1143
Cuts at: 4405 4787 5422 275
Size: 382 635 1451
Fragments arranged by size:
1451 1143 936 635 620 607 502 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2325 3346 5031 5429 5703 5850 6261 2325
Size: 1021 1685 398 274 147 411 2662
Fragments arranged by size:
2662 1685 1021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2244 2274 3479 3509 3750
Size: 30 165 30 841 30 1205 30 241
Cuts at: 3750 3780 1178
Size: 30 3996
Fragments arranged by size:
3996 1205 841 241 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 2549 2634 2637 3425 3482 3860 1186
Size: 1321 42 85 3 788 57 378 3924
Fragments arranged by size:
3924 1321 788 378 85 57 42 3
BseYI C'CCAG_C
Cuts at: 2142 2704 3195 3740 3809 5801 6297 2142
Size: 562 491 545 69 1992 496 2443
Fragments arranged by size:
2443 1992 562 545 496 491 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2361 3059 3353 3708 1163
Size: 573 625 698 294 355 4053
Fragments arranged by size:
4053 698 625 573 355 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2366 2611 4048 4809 5837 1540
Size: 275 186 365 245 1437 761 1028 2301
Fragments arranged by size:
2301 1437 1028 761 365 275 245 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2803 2822 4659 4744 5815 2166
Size: 637 19 1837 85 1071 2949
Fragments arranged by size:
2949 1837 1071 637 85 19
BsmI GAATG_Cn'
Cuts at: 4109 4202 4109
Size: 93 6505
BsmBI CGTCTCn'nnnn_
Cuts at: 2264 2381 3175 2264
Size: 117 794 5687
Fragments arranged by size:
5687 794 117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2166 2765 2803 2822 3067 3333
Size: 399 352 24 599 38 19 245 266
Cuts at: 3333 3678 3882 3917 4659 4744 5815 1391
Size: 345 204 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 599 399 352 345 266
245 204 85 38 35 24 19
BspEI T'CCGG_A
Cuts at: 2325 3346 6261 2325
Size: 1021 2915 2662
Fragments arranged by size:
2915 2662 1021
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 2637 3953 844
Size: 1336 457 1316 3489
Fragments arranged by size:
3489 1336 1316 457
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 5242 5429 1700
Size: 110 800 2632 187 2869
Fragments arranged by size:
2869 2632 800 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6598
BssHII G'CGCG_C
Cuts at: 1925 3238 1925
Size: 1313 5285
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2241 3046 3722 3731 794
Size: 1447 805 676 9 3661
Fragments arranged by size:
3661 1447 805 676 9
BstBI TT'CG_AA
Cuts at: 5402 5402
Size: 6598
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2212 2185
Size: 27 6571
BstYI r'GATC_y
Cuts at: 1972 2829 3928 4295 4680 4697 6149 6235 6247
Size: 857 1099 367 385 17 1452 86 12
Cuts at: 6247 6593 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1099 857 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2453 3377 2453
Size: 924 5674
BtgI C'CryG_G
Cuts at: 513 1065 2148 3243 3526 3594 3765 513
Size: 552 1083 1095 283 68 171 3346
Fragments arranged by size:
3346 1095 1083 552 283 171 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5859
Fragments arranged by size:
5859 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 4114 4908 4928 2378
Size: 1736 794 20 4048
Fragments arranged by size:
4048 1736 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2507 5605 809
Size: 507 1191 3098 1802
Fragments arranged by size:
3098 1802 1191 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2363 2493
Size: 54 1425 50 275 18 391 142 130
Cuts at: 2493 2608 2612 2895 3246 3597 3637 4045 4934
Size: 115 4 283 351 351 40 408 889
Cuts at: 4934 6563 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 408 391 351 351 283
275 142 130 115 54 50 43 40
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2363 2608 4045 1537
Size: 275 551 245 1437 4090
Fragments arranged by size:
4090 1437 551 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 3888 4528 1353
Size: 956 1579 640 3423
Fragments arranged by size:
3423 1579 956 640
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2420 3115 3137 5185 5559 5705
Size: 1064 138 831 695 22 2048 374 146
Cuts at: 5705 387
Size: 1280
Fragments arranged by size:
2048 1280 1064 831 695 374 146 138
22
EcoICRI GAG'CTC
Cuts at: 2164 2820 2164
Size: 656 5942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2230 2501 2558 2612 2873 2897
Size: 393 575 23 271 57 54 261 24
Cuts at: 2897 3777 3872 4661 5258 6045 6313 1239
Size: 880 95 789 597 787 268 1524
Fragments arranged by size:
1524 880 789 787 597 575 393 271
268 261 95 57 54 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3087 3380 3887 3087
Size: 293 507 5798
Fragments arranged by size:
5798 507 293
EcoO109I rG'GnC_Cy
Cuts at: 2138 2139 2320 2443 3162 3329 3466 2138
Size: 1 181 123 719 167 137 5270
Fragments arranged by size:
5270 719 181 167 137 123 1
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FseI GG_CCGG'CC
Cuts at: 2614 2614
Size: 6598
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 2156 2658 5745 1678
Size: 478 502 3087 2531
Fragments arranged by size:
3087 2531 502 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 3252 3284 3346
Size: 32 135 32 620 32 1028 32 62
Cuts at: 3346 3378 5239 5271 5313 5345 1373
Size: 32 1861 32 42 32 2626
Fragments arranged by size:
2626 1861 1028 620 135 62 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3942 4188 669
Size: 988 281 2004 246 3079
Fragments arranged by size:
3079 2004 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KasI G'GCGC_C
Cuts at: 2152 2654 2152
Size: 502 6096
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 5712 5896 1305
Size: 798 3609 184 2007
Fragments arranged by size:
3609 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2223 2495 3248 3639 10
Size: 54 1425 343 391 272 753 391 2969
Fragments arranged by size:
2969 1425 753 391 391 343 272 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 2810 4556 4915 5074 518
Size: 648 1515 129 1746 359 159 2042
Fragments arranged by size:
2042 1746 1515 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2088 2362 2368 2413 2475 3213 3367 3722
Size: 482 274 6 45 62 738 154 355
Cuts at: 3722 3785 4014 4691 5839 6084 1606
Size: 63 229 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 482 355 274 245
229 154 63 62 45 6
NaeI GCC'GGC
Cuts at: 2612 2612
Size: 6598
NarI GG'CG_CC
Cuts at: 2153 2655 2153
Size: 502 6096
NcoI C'CATG_G
Cuts at: 513 1065 2148 3526 513
Size: 552 1083 1378 3585
Fragments arranged by size:
3585 1378 1083 552
NdeI CA'TA_TG
Cuts at: 387 2678 387
Size: 2291 4307
NgoMIV G'CCGG_C
Cuts at: 2610 2610
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 3283 1052
Size: 2231 4367
NotI GC'GGCC_GC
Cuts at: 2363 4045 2363
Size: 1682 4916
NspI r_CATG'y
Cuts at: 1132 1229 2996 3956 5501 1132
Size: 97 1767 960 1545 2229
Fragments arranged by size:
2229 1767 1545 960 97
PasI CC'CwG_GG
Cuts at: 1348 3089 1348
Size: 1741 4857
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 2941 3157 3532 1108
Size: 1041 792 216 375 4174
Fragments arranged by size:
4174 1041 792 375 216
PfoI T'CCnGG_A
Cuts at: 3888 3888
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PmlI CAC'GTG
Cuts at: 2893 2893
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4667 4699 6180 6212 3479
Size: 32 1156 32 1481 32 3865
Fragments arranged by size:
3865 1481 1156 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3466 3466
Size: 6598
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2138 3329 2138
Size: 1191 5407
PstI C_TGCA'G
Cuts at: 830 2385 2640 2648 3591 3736 3950 830
Size: 1555 255 8 943 145 214 3478
Fragments arranged by size:
3478 1555 943 255 214 145 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 3367 3722 3367
Size: 355 6243
SacI G_AGCT'C
Cuts at: 2166 2822 2166
Size: 656 5942
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SbfI CC_TGCA'GG
Cuts at: 2640 3950 2640
Size: 1310 5288
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 2381 2636 2644 3587 3732 3946 5081
Size: 219 1336 255 8 943 145 214 1135
Cuts at: 5081 5762 5953 826
Size: 681 191 1471
Fragments arranged by size:
1471 1336 1135 943 681 255 219 214
191 145 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 3779 3779
Size: 6598
SfoI GGC'GCC
Cuts at: 2154 2656 2154
Size: 502 6096
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 2159 2265 3925 1872
Size: 287 106 1660 4545
Fragments arranged by size:
4545 1660 287 106
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4702 5603 5865 820
Size: 197 926 2759 901 262 1553
Fragments arranged by size:
2759 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 2133 3605 1517
Size: 616 1472 4510
Fragments arranged by size:
4510 1472 616
StyI C'CwwG_G
Cuts at: 513 1065 2009 2148 3033 3109 3526 3564 3600
Size: 552 944 139 885 76 417 38 36
Cuts at: 3600 4037 513
Size: 437 3074
Fragments arranged by size:
3074 944 885 552 437 417 139 76
38 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3692 3794 3888 4625 4642 4795
Size: 1255 1935 177 102 94 737 17 153
Cuts at: 4795 4980 5425 325
Size: 185 445 1498
Fragments arranged by size:
1935 1498 1255 737 445 185 177 153
102 94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4844 6556 96
Size: 274 80 33 51 1443 2867 1712 138
Fragments arranged by size:
2867 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 3750 3782 6558 6590 2244
Size: 32 1474 32 2776 32 2252
Fragments arranged by size:
2776 2252 1474 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3122 3607 3017
Size: 105 485 6008
Fragments arranged by size:
6008 485 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 2157 2263 3923 1870
Size: 287 106 1660 4545
Fragments arranged by size:
4545 1660 287 106
XmnI GAAnn'nnTTC
Cuts at: 2552 4727 2552
Size: 2175 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SexAI SrfI SwaI