Restriction map for
pFN21AE1125
(Circular) (Six-base) MAPSORT of: pFN21AE1125.seq Check: 7673 from: 1 to: 6421
pFN21AE1125 6421 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3245 3350 3245
Size: 105 6316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4231 278
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3764 1937
Size: 1827 4594
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3083 4484 5868 6136 1632
Size: 1451 1401 1384 268 1917
Fragments arranged by size:
1917 1451 1401 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 5320 6297 1203
Size: 4117 977 1327
Fragments arranged by size:
4117 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 2455 5150 2455
Size: 2695 3726
AleI CACnn'nnGTG
Cuts at: 1166 3246 3413 1166
Size: 2080 167 4174
Fragments arranged by size:
4174 2080 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 2629 4164 5736 2629
Size: 1535 1572 3314
Fragments arranged by size:
3314 1572 1535
ApaI G_GGCC'C
Cuts at: 2161 2769 3046 3054 2161
Size: 608 277 8 5528
Fragments arranged by size:
5528 608 277 8
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2153 2502 2760 2973 3180 3508
Size: 73 183 27 349 258 213 207 328
Cuts at: 3508 3746 1870
Size: 238 4545
Fragments arranged by size:
4545 349 328 258 238 213 207 183
73 27
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2149 2276 2895 3292 3440
Size: 325 217 627 362 127 619 397 148
Cuts at: 3440 3742 5197 618
Size: 302 1455 1842
Fragments arranged by size:
1842 1455 627 619 397 362 325 302
217 148 127
BanII G_rGCy'C
Cuts at: 1391 2161 2769 2857 3046 3054 3354 3534 3715
Size: 770 608 88 189 8 300 180 181
Cuts at: 3715 3740 1391
Size: 25 4072
Fragments arranged by size:
4072 770 608 300 189 181 180 88
25 8
BbeI G_GCGC'C
Cuts at: 3296 3296
Size: 6421
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 928
Size: 971 318 5132
Fragments arranged by size:
5132 971 318
BbvCI CC'TCA_GC
Cuts at: 3371 3371
Size: 6421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2184 2764
Size: 107 122 71 631 605 337 175 580
Cuts at: 2764 5032 136
Size: 2268 1525
Fragments arranged by size:
2268 1525 631 605 580 337 175 122
107 71
BglII A'GATC_T
Cuts at: 2340 6416 2340
Size: 4076 2345
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2526 2585 2769 2898 3003 3046 3054
Size: 371 365 59 184 129 105 43 8
Cuts at: 3054 3443 4482 5638 1790
Size: 389 1039 1156 2573
Fragments arranged by size:
2573 1156 1039 389 371 365 184 129
105 59 43 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 3460 2467
Size: 993 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2772 2790 2871 3101 3245 3305 3347
Size: 1112 421 18 81 230 144 60 42
Cuts at: 3347 3455 5081 1239
Size: 108 1626 2579
Fragments arranged by size:
2579 1626 1112 421 230 144 108 81
60 42 18
Bpu10I CC'TnA_GC
Cuts at: 3371 3543 3371
Size: 172 6249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4546 5411 5709 4546
Size: 865 298 5258
Fragments arranged by size:
5258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2050 5084 882
Size: 617 551 3034 2219
Fragments arranged by size:
3034 2219 617 551
BsaAI yAC'GTr
Cuts at: 493 1204 3682 4212 493
Size: 711 2478 530 2702
Fragments arranged by size:
2702 2478 711 530
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3293 4228 4610 5245
Size: 53 83 186 936 1760 935 382 635
Cuts at: 5245 275
Size: 1451
Fragments arranged by size:
1760 1451 936 935 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2365 4854 5252 5526 5673 6084 2365
Size: 2489 398 274 147 411 2702
Fragments arranged by size:
2702 2489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6196
Fragments arranged by size:
6196 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2351 2352 2384 2387 3089 1186
Size: 1162 3 1 32 3 702 4518
Fragments arranged by size:
4518 1162 702 32 3 3 1
BseYI C'CCAG_C
Cuts at: 2160 2240 2622 2682 2688 2730 2754 2770 2939
Size: 80 382 60 6 42 24 16 169
Cuts at: 2939 2955 3093 5624 6120 2160
Size: 16 138 2531 496 2461
Fragments arranged by size:
2531 2461 496 382 169 138 80 60
42 24 16 16 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2999 1163
Size: 573 1263 4585
Fragments arranged by size:
4585 1263 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2304 3160 3871 4632 5660 1540
Size: 275 186 303 856 711 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 711 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 2269 3518 3687 4482 4567 5638 2269
Size: 1249 169 795 85 1071 3052
Fragments arranged by size:
3052 1249 1071 795 169 85
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 2028 2028
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2269 2526 2585 2769 2857 2898
Size: 399 371 108 257 59 184 88 41
Cuts at: 2898 3003 3046 3054 3354 3443 3518 3534 3687
Size: 105 43 8 300 89 75 16 153
Cuts at: 3687 3715 3740 4482 4567 5638 1391
Size: 28 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 300 257 184
153 108 105 89 88 85 75 59
43 41 28 25 16 8
BspEI T'CCGG_A
Cuts at: 2365 6084 2365
Size: 3719 2702
BspHI T'CATG_A
Cuts at: 4310 6040 4310
Size: 1730 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3245 3350 3776 844
Size: 2401 105 426 3489
Fragments arranged by size:
3489 2401 426 105
BsrBI CCG'CTC
Cuts at: 1394 1823 4308 1394
Size: 429 2485 3507
Fragments arranged by size:
3507 2485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4916 5090 65
Size: 1183 3668 174 1396
Fragments arranged by size:
3668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3007 3468 5065 5252 1700
Size: 110 1197 461 1597 187 2869
Fragments arranged by size:
2869 1597 1197 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 2164 1925
Size: 239 6182
BssSI C'ACGA_G
Cuts at: 4481 5493 4481
Size: 1012 5409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2264 2998 3103 794
Size: 1470 734 105 4112
Fragments arranged by size:
4112 1470 734 105
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2340 2864 3751 4118 4503 4520 5972 6058
Size: 368 524 887 367 385 17 1452 86
Cuts at: 6058 6070 6416 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 887 524 385 368 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2700 2385
Size: 315 6106
BtgI C'CryG_G
Cuts at: 513 1065 2004 3378 513
Size: 552 939 1374 3556
Fragments arranged by size:
3556 1374 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3208 487
Size: 683 56 1982 3700
Fragments arranged by size:
3700 1982 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 3400 3702 3937 4731 4751 2462
Size: 938 302 235 794 20 4132
Fragments arranged by size:
4132 938 794 302 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DraIII CAC_nnn'GTG
Cuts at: 2998 2998
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 3157 3550
Size: 54 1425 50 275 18 705 622 393
Cuts at: 3550 3868 4757 6386 8
Size: 318 889 1629 43
Fragments arranged by size:
1629 1425 889 705 622 393 318 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 3868 1537
Size: 275 1345 711 4090
Fragments arranged by size:
4090 1345 711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4351 1353
Size: 2998 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2772 2790 2871 3083 3101 3245
Size: 393 719 421 18 81 212 18 144
Cuts at: 3245 3305 3347 3455 4484 5081 5868 6136 1239
Size: 60 42 108 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 787 719 597 421 393 268
212 144 108 81 60 42 18 18
EcoO109I rG'GnC_Cy
Cuts at: 2157 2381 2389 2765 2766 3042 3043 3050 3051
Size: 224 8 376 1 276 1 7 1
Cuts at: 3051 2157
Size: 5527
Fragments arranged by size:
5527 376 276 224 8 7 1 1
1
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FspI TGC'GCA
Cuts at: 3252 4927 3252
Size: 1675 4746
HaeII r_GCGC'y
Cuts at: 1678 3296 5568 1678
Size: 1618 2272 2531
Fragments arranged by size:
2531 2272 1618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2437 2441 2469 2473 5062
Size: 32 135 32 865 4 28 4 2589
Cuts at: 5062 5094 5136 5168 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2589 865 135 42 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3145 3765 4011 669
Size: 988 281 1207 620 246 3079
Fragments arranged by size:
3079 1207 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KasI G'GCGC_C
Cuts at: 3292 3292
Size: 6421
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2731 3671 5535 5719 1305
Size: 1426 940 1864 184 2007
Fragments arranged by size:
2007 1864 1426 940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 10
Size: 54 1425 343 705 3894
Fragments arranged by size:
3894 1425 705 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3246 3413 4379 4738 4897 518
Size: 648 2080 167 966 359 159 2042
Fragments arranged by size:
2080 2042 966 648 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 3088 3214 3837 4514 5662 5907 1606
Size: 1482 126 623 677 1148 245 2120
Fragments arranged by size:
2120 1482 1148 677 623 245 126
NarI GG'CG_CC
Cuts at: 3293 3293
Size: 6421
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4930
Fragments arranged by size:
4930 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 3370 3779 5324 1132
Size: 97 2141 409 1545 2229
Fragments arranged by size:
2229 2141 1545 409 97
PasI CC'CwG_GG
Cuts at: 1348 2415 3038 3047 1348
Size: 1067 623 9 4722
Fragments arranged by size:
4722 1067 623 9
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6421
PfoI T'CCnGG_A
Cuts at: 2848 2848
Size: 6421
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PmlI CAC'GTG
Cuts at: 3682 3682
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2157 2765 3042 3050 2157
Size: 608 277 8 5528
Fragments arranged by size:
5528 608 277 8
PstI C_TGCA'G
Cuts at: 830 2575 3773 830
Size: 1745 1198 3478
Fragments arranged by size:
3478 1745 1198
PvuI CG_AT'CG
Cuts at: 2001 2304 2001
Size: 303 6118
PvuII CAG'CTG
Cuts at: 3088 3214 3088
Size: 126 6295
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SexAI A'CCwGG_T
Cuts at: 3024 3024
Size: 6421
SfcI C'TryA_G
Cuts at: 826 1045 2571 3277 3769 4904 5585 5776 826
Size: 219 1526 706 492 1135 681 191 1471
Fragments arranged by size:
1526 1471 1135 706 681 492 219 191
SfoI GGC'GCC
Cuts at: 3294 3294
Size: 6421
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 2128 2155 2504 3182 3748 1872
Size: 256 27 349 678 566 4545
Fragments arranged by size:
4545 678 566 349 256 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4525 5426 5688 820
Size: 197 926 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3370 3779 1132
Size: 2238 409 3774
Fragments arranged by size:
3774 2238 409
SrfI GCCC'GGGC
Cuts at: 3182 3182
Size: 6421
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 3389 1517
Size: 1872 4549
StyI C'CwwG_G
Cuts at: 513 1065 2004 2600 3860 513
Size: 552 939 596 1260 3074
Fragments arranged by size:
3074 1260 939 596 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2768 4448 4465 4618 4803 5248
Size: 1255 452 736 1680 17 153 185 445
Cuts at: 5248 325
Size: 1498
Fragments arranged by size:
1680 1498 1255 736 452 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4667 6379 96
Size: 274 80 33 51 1443 2690 1712 138
Fragments arranged by size:
2690 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6381 6413 6381
Size: 32 6389
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6421
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 2126 2153 2502 3180 3746 1870
Size: 256 27 349 678 566 4545
Fragments arranged by size:
4545 678 566 349 256 27
XmnI GAAnn'nnTTC
Cuts at: 2078 2117 2214 2313 4550 2078
Size: 39 97 99 2237 3949
Fragments arranged by size:
3949 2237 99 97 39
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4229 276
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SwaI XcmI