Restriction map for
pFN21AE1124
(Circular) (Six-base) MAPSORT of: pFN21AE1124.seq Check: 3591 from: 1 to: 6112
pFN21AE1124 6112 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3034 3034
Size: 6112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2823 3433 2823
Size: 610 5502
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2931 4175 5559 5827 1632
Size: 1299 1244 1384 268 1917
Fragments arranged by size:
1917 1384 1299 1244 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 2470 4841 2470
Size: 2371 3741
AleI CACnn'nnGTG
Cuts at: 1166 2396 1166
Size: 1230 4882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 1184
Size: 1191 4921
AlwNI CAG_nnn'CTG
Cuts at: 2893 3016 3202 3855 5427 2893
Size: 123 186 653 1572 3578
Fragments arranged by size:
3578 1572 653 186 123
ApaLI G'TGCA_C
Cuts at: 3210 4169 5325 3210
Size: 959 1156 3997
Fragments arranged by size:
3997 1156 959
ApoI r'AATT_y
Cuts at: 1493 2798 3421 3647 3799 5908 1493
Size: 1305 623 226 152 2109 1697
Fragments arranged by size:
2109 1697 1305 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2585 2722 3386 3437 1870
Size: 73 390 252 137 664 51 4545
Fragments arranged by size:
4545 664 390 252 137 73 51
BamHI G'GATC_C
Cuts at: 2152 3442 3809 2152
Size: 1290 367 4455
Fragments arranged by size:
4455 1290 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2558 2823 3118 3310
Size: 325 217 627 620 151 265 295 192
Cuts at: 3310 3433 4888 618
Size: 123 1455 1842
Fragments arranged by size:
1842 1455 627 620 325 295 265 217
192 151 123
BanII G_rGCy'C
Cuts at: 1391 2063 2988 3431 1391
Size: 672 925 443 4072
Fragments arranged by size:
4072 925 672 443
BbeI G_GCGC'C
Cuts at: 3314 3314
Size: 6112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2112 2427 3075 928
Size: 971 159 54 315 648 3965
Fragments arranged by size:
3965 971 648 315 159 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 4723 136
Size: 107 122 71 631 605 568 2483 1525
Fragments arranged by size:
2483 1525 631 605 568 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 3214 3340 4173 5329 1790
Size: 1424 126 833 1156 2573
Fragments arranged by size:
2573 1424 1156 833 126
BmgBI CAC'GTC
Cuts at: 1318 2349 1318
Size: 1031 5081
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2066 3843 4801
Size: 416 53 83 399 24 649 1777 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1777 1753 958 649 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 3152 3257 4772 1239
Size: 1019 894 105 1515 2579
Fragments arranged by size:
2579 1515 1019 894 105
Bpu10I CC'TnA_GC
Cuts at: 2057 2057
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3181 3301 3903 493
Size: 711 1977 120 602 2702
Fragments arranged by size:
2702 1977 711 602 120
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2380 3311 3919 4301
Size: 53 83 186 936 847 931 608 382
Cuts at: 4301 4936 275
Size: 635 1451
Fragments arranged by size:
1451 936 931 847 635 608 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2778 4545 4943 5217 5364 5775 2778
Size: 1767 398 274 147 411 3115
Fragments arranged by size:
3115 1767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2918 2948 2956 2986 1178
Size: 30 165 30 1515 30 8 30 4304
Fragments arranged by size:
4304 1515 165 30 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3223 1186
Size: 2037 4075
BseYI C'CCAG_C
Cuts at: 2566 5315 5811 2566
Size: 2749 496 2867
Fragments arranged by size:
2867 2749 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 2723 3002 3068 3323 1163
Size: 573 876 111 279 66 255 3952
Fragments arranged by size:
3952 876 573 279 255 111 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3333 3348 3562 4323 5351 1540
Size: 275 186 1332 15 214 761 1028 2301
Fragments arranged by size:
2301 1332 1028 761 275 214 186 15
BsiHKAI G_wGCw'C
Cuts at: 2966 2988 3214 3393 4173 4258 5329 2966
Size: 22 226 179 780 85 1071 3749
Fragments arranged by size:
3749 1071 780 226 179 85 22
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2966 2988 3214 3340 3393 3431
Size: 399 273 903 22 226 126 53 38
Cuts at: 3431 4173 4258 5329 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 903 742 399 273 226 126
85 53 38 22
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 3034 3467 844
Size: 1278 912 433 3489
Fragments arranged by size:
3489 1278 912 433
BsrBI CCG'CTC
Cuts at: 1394 1823 2448 2761 3999 1394
Size: 429 625 313 1238 3507
Fragments arranged by size:
3507 1238 625 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 4756 4943 1700
Size: 110 511 2435 187 2869
Fragments arranged by size:
2869 2435 511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 2432 4172 5184 2432
Size: 1740 1012 3360
Fragments arranged by size:
3360 1740 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2643 3016 794
Size: 1849 373 3890
Fragments arranged by size:
3890 1849 373
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2152 2272 3051 3171 3442 3809 4194 4211
Size: 180 120 779 120 271 367 385 17
Cuts at: 4211 5663 5749 5761 6107 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 779 385 367 346 271 180
120 120 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2313 3225 3243 513
Size: 552 1248 912 18 3382
Fragments arranged by size:
3382 1248 912 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2377 3094 487
Size: 683 56 1151 717 3505
Fragments arranged by size:
3505 1151 717 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 3628 4422 4442 2052
Size: 1576 794 20 3722
Fragments arranged by size:
3722 1576 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DraIII CAC_nnn'GTG
Cuts at: 2865 2865
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2319 3030
Size: 54 1425 50 275 18 195 294 711
Cuts at: 3030 3330 3345 3559 4448 6077 8
Size: 300 15 214 889 1629 43
Fragments arranged by size:
1629 1425 889 711 300 294 275 214
195 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3330 3345 3559 1537
Size: 275 1518 15 214 4090
Fragments arranged by size:
4090 1518 275 214 15
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 2693 4042 1353
Size: 1182 158 1349 3423
Fragments arranged by size:
3423 1349 1182 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 2986
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2931 3152 3257 4175 4772 5559
Size: 393 626 673 221 105 918 597 787
Cuts at: 5559 5827 1239
Size: 268 1524
Fragments arranged by size:
1524 918 787 673 626 597 393 268
221 105
EcoO109I rG'GnC_Cy
Cuts at: 2308 3013 2308
Size: 705 5407
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2669 3314 5259 1678
Size: 991 645 1945 2531
Fragments arranged by size:
2531 1945 991 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2456 2488 4753
Size: 32 135 32 552 32 300 32 2265
Cuts at: 4753 4785 4827 4859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2265 552 300 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KasI G'GCGC_C
Cuts at: 3310 3310
Size: 6112
KpnI G_GTAC'C
Cuts at: 2827 3437 2827
Size: 610 5502
MfeI C'AATT_G
Cuts at: 2143 3711 2143
Size: 1568 4544
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 2187 2643 2739 2841 5226 5410 1305
Size: 687 195 456 96 102 2385 184 2007
Fragments arranged by size:
2385 2007 687 456 195 184 102 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3032 10
Size: 54 1425 343 1200 3090
Fragments arranged by size:
3090 1425 1200 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2399 4070 4429 4588 518
Size: 648 1230 3 1671 359 159 2042
Fragments arranged by size:
2042 1671 1230 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2478 2752 2881 3217 3245 3307 3528 4205
Size: 872 274 129 336 28 62 221 677
Cuts at: 4205 5353 5598 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 336 274 245 221
129 62 28
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 6112
NarI GG'CG_CC
Cuts at: 3311 3311
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 2313 513
Size: 552 1248 4312
Fragments arranged by size:
4312 1248 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2626 3470 5015 1132
Size: 97 1397 844 1545 2229
Fragments arranged by size:
2229 1545 1397 844 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 3181 1108
Size: 1785 288 4039
Fragments arranged by size:
4039 1785 288
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PmlI CAC'GTG
Cuts at: 3181 3181
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 6112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspXI vC'TCGA_Gb
Cuts at: 3386 3386
Size: 6112
PstI C_TGCA'G
Cuts at: 830 3207 3360 3464 830
Size: 2377 153 104 3478
Fragments arranged by size:
3478 2377 153 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2478 2752 3217 3307 2478
Size: 274 465 90 5283
Fragments arranged by size:
5283 465 274 90
SacI G_AGCT'C
Cuts at: 2988 2988
Size: 6112
SacII CC_GC'GG
Cuts at: 3246 3246
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 3203 3356 3368 3460 4595 5276 5467
Size: 219 2158 153 12 92 1135 681 191
Cuts at: 5467 826
Size: 1471
Fragments arranged by size:
2158 1471 1135 681 219 191 153 92
12
SfoI GGC'GCC
Cuts at: 3312 3312
Size: 6112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3386 4216 5117 5379 820
Size: 197 926 1443 830 901 262 1553
Fragments arranged by size:
1553 1443 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2313 3551 513
Size: 552 1248 1238 3074
Fragments arranged by size:
3074 1248 1238 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 4139 4156 4309 4494 4939 325
Size: 1255 505 2054 17 153 185 445 1498
Fragments arranged by size:
2054 1498 1255 505 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2956 2988 6072 6104 2956
Size: 32 3084 32 2964
Fragments arranged by size:
3084 2964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 3386 1943
Size: 1443 4669
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI