Restriction map for
pFN21AE1123
(Circular) (Six-base) MAPSORT of: pFN21AE1123.seq Check: 2994 from: 1 to: 6073
pFN21AE1123 6073 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2607 2607
Size: 6073
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 3416 1937
Size: 1164 315 4594
Fragments arranged by size:
4594 1164 315
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2676 4136 5520 5788 1632
Size: 1044 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 1044 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 2263 3232 3816 5388 2263
Size: 969 584 1572 2948
Fragments arranged by size:
2948 1572 969 584
ApaLI G'TGCA_C
Cuts at: 2687 4130 5286 2687
Size: 1443 1156 3474
Fragments arranged by size:
3474 1443 1156
ApoI r'AATT_y
Cuts at: 1493 2639 3382 3608 3760 5869 1493
Size: 1146 743 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2168 2841 4627 160
Size: 872 1136 673 1786 1606
Fragments arranged by size:
1786 1606 1136 872 673
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2890 3398 1870
Size: 73 947 508 4545
Fragments arranged by size:
4545 947 508 73
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3197 3394 4849 618
Size: 325 217 627 1410 197 1455 1842
Fragments arranged by size:
1842 1455 1410 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 3392 1391
Size: 2001 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 2333 2813 2958 928
Size: 971 143 291 480 145 4043
Fragments arranged by size:
4043 971 480 291 145 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 5146
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 4684 136
Size: 107 122 71 631 605 433 2579 1525
Fragments arranged by size:
2579 1525 631 605 433 122 107 71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 2880 3490 2880
Size: 610 5463
Bme1580I G_kGCm'C
Cuts at: 1790 2691 3142 4134 5290 1790
Size: 901 451 992 1156 2573
Fragments arranged by size:
2573 1156 992 901 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3804 4762 442
Size: 416 53 83 399 24 2387 958 1753
Fragments arranged by size:
2387 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2586 2981 4733 1239
Size: 1347 395 1752 2579
Fragments arranged by size:
2579 1752 1347 395
Bpu10I CC'TnA_GC
Cuts at: 2256 2256
Size: 6073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 4736 882
Size: 617 876 2361 2219
Fragments arranged by size:
2361 2219 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 2065 3864 493
Size: 711 861 1799 2702
Fragments arranged by size:
2702 1799 861 711
BsaBI GATnn'nnATC
Cuts at: 3083 6067 3083
Size: 2984 3089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 3880 4262 4897
Size: 53 83 186 936 621 1726 382 635
Cuts at: 4897 275
Size: 1451
Fragments arranged by size:
1726 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3087 4506 4904 5178 5325 5736 3087
Size: 1419 398 274 147 411 3424
Fragments arranged by size:
3424 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5848
Fragments arranged by size:
5848 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 3182 1186
Size: 844 1152 4077
Fragments arranged by size:
4077 1152 844
BseYI C'CCAG_C
Cuts at: 2074 2263 2525 5276 5772 2074
Size: 189 262 2751 496 2375
Fragments arranged by size:
2751 2375 496 262 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 3245 1163
Size: 573 1197 312 3991
Fragments arranged by size:
3991 1197 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2691 4134 4219 5290 2030
Size: 661 1443 85 1071 2813
Fragments arranged by size:
2813 1443 1071 661 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2691 3142 3392 4134 4219 5290
Size: 399 240 661 451 250 742 85 1071
Cuts at: 5290 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 661 451 399 250 240
85
BspEI T'CCGG_A
Cuts at: 3087 5736 3087
Size: 2649 3424
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 2955 3428 844
Size: 1763 348 473 3489
Fragments arranged by size:
3489 1763 473 348
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 3960 1394
Size: 429 268 1869 3507
Fragments arranged by size:
3507 1869 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 4568 4742 65
Size: 1183 1626 1694 174 1396
Fragments arranged by size:
1694 1626 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 3343 3343
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 3403 3770 4155 4172 5624 5710 5722 6068
Size: 1431 367 385 17 1452 86 12 346
Cuts at: 6068 1972
Size: 1977
Fragments arranged by size:
1977 1452 1431 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3102 3102
Size: 6073
Bsu36I CC'TnA_GG
Cuts at: 2113 2113
Size: 6073
BtgI C'CryG_G
Cuts at: 513 1065 2442 513
Size: 552 1377 4144
Fragments arranged by size:
4144 1377 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2032 487
Size: 683 56 806 4528
Fragments arranged by size:
4528 806 683 56
BtsI GCAGTG_nn'
Cuts at: 3589 4383 4403 3589
Size: 794 20 5259
Fragments arranged by size:
5259 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3240 3520
Size: 54 1425 50 275 18 267 1143 280
Cuts at: 3520 4409 6038 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1143 889 280 275 267 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2188 2600 2652 3291 4003 1353
Size: 665 170 412 52 639 712 3423
Fragments arranged by size:
3423 712 665 639 412 170 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3322 4660 5034 5180 387
Size: 1064 138 1733 1338 374 146 1280
Fragments arranged by size:
1733 1338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2586 2676 2981 4136 4733 5520 5788
Size: 393 954 90 305 1155 597 787 268
Cuts at: 5788 1239
Size: 1524
Fragments arranged by size:
1524 1155 954 787 597 393 305 268
90
EcoO109I rG'GnC_Cy
Cuts at: 2213 2275 2213
Size: 62 6011
EcoRI G'AATT_C
Cuts at: 2639 3382 3760 2639
Size: 743 378 4952
Fragments arranged by size:
4952 743 378
EcoRV GAT'ATC
Cuts at: 2770 2770
Size: 6073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 6073
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 2754 2759 2786
Size: 32 135 32 850 32 300 5 27
Cuts at: 2786 2791 3023 3055 3311 3343 4714 4746 4788
Size: 5 232 32 256 32 1371 32 42
Cuts at: 4788 4820 1373
Size: 32 2626
Fragments arranged by size:
2626 1371 850 300 256 232 135 42
32 32 32 32 32 32 32 27
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 5187 5371 1305
Size: 1716 2166 184 2007
Fragments arranged by size:
2166 2007 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 3984
Fragments arranged by size:
3984 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4031 4390 4549 518
Size: 648 2865 359 159 2042
Fragments arranged by size:
2865 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3229 3489 4166 5314 5559 1606
Size: 1623 260 677 1148 245 2120
Fragments arranged by size:
2120 1623 1148 677 260 245
NcoI C'CATG_G
Cuts at: 513 1065 2442 513
Size: 552 1377 4144
Fragments arranged by size:
4144 1377 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 2151 2923 3293 3431 4976 1132
Size: 97 922 772 370 138 1545 2229
Fragments arranged by size:
2229 1545 922 772 370 138 97
PasI CC'CwG_GG
Cuts at: 1348 2755 1348
Size: 1407 4666
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 1108
Size: 1815 4258
PfoI T'CCnGG_A
Cuts at: 2218 2218
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PmlI CAC'GTG
Cuts at: 2065 2065
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2213 2275 2213
Size: 62 6011
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PstI C_TGCA'G
Cuts at: 830 3425 830
Size: 2595 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 3229 3229
Size: 6073
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 2188 2018
Size: 170 5903
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 3421 4556 5237 5428 826
Size: 219 2376 1135 681 191 1471
Fragments arranged by size:
2376 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 2892 3400 1872
Size: 1020 508 4545
Fragments arranged by size:
4545 1020 508
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4177 5078 5340 820
Size: 197 926 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2385 2442 3512 513
Size: 552 1320 57 1070 3074
Fragments arranged by size:
3074 1320 1070 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3087 4100 4117 4270 4455 4900 325
Size: 1255 1507 1013 17 153 185 445 1498
Fragments arranged by size:
1507 1498 1255 1013 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3363 4319
Size: 274 80 33 51 1443 882 504 956
Cuts at: 4319 6031 96
Size: 1712 138
Fragments arranged by size:
1712 1443 956 882 504 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2918 2950 6033 6065 2918
Size: 32 3083 32 2926
Fragments arranged by size:
3083 2926 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 2890 3398 1870
Size: 1020 508 4545
Fragments arranged by size:
4545 1020 508
XmnI GAAnn'nnTTC
Cuts at: 4202 4202
Size: 6073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI