Restriction map for
pFN21AE1121
(Circular) (Six-base) MAPSORT of: pFN21AE1121.seq Check: 1403 from: 1 to: 5803
pFN21AE1121 5803 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2304 2900 2304
Size: 596 5207
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2784 3866 5250 5518 1632
Size: 1152 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1152 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 2171 1166
Size: 1005 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2114 2312 1184
Size: 930 198 4675
Fragments arranged by size:
4675 930 198
AlwNI CAG_nnn'CTG
Cuts at: 2014 2086 2323 2572 2593 3546 5118 2014
Size: 72 237 249 21 953 1572 2699
Fragments arranged by size:
2699 1572 953 249 237 72 21
ApaI G_GGCC'C
Cuts at: 2182 2434 2633 2182
Size: 252 199 5352
Fragments arranged by size:
5352 252 199
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2427 2433 2860 3128 1870
Size: 73 114 370 6 427 268 4545
Fragments arranged by size:
4545 427 370 268 114 73 6
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2645 2680 2783 2888
Size: 325 217 627 335 523 35 103 105
Cuts at: 2888 3124 4579 618
Size: 236 1455 1842
Fragments arranged by size:
1842 1455 627 523 335 325 236 217
105 103 35
BanII G_rGCy'C
Cuts at: 1391 2182 2434 2633 2703 2746 2859 3122 1391
Size: 791 252 199 70 43 113 263 4072
Fragments arranged by size:
4072 791 263 252 199 113 70 43
BbeI G_GCGC'C
Cuts at: 2649 2684 2649
Size: 35 5768
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3994 4028 1903
Size: 34 50 34 1973 34 3678
Fragments arranged by size:
3678 1973 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2689 3091
Size: 107 122 71 631 605 345 672 402
Cuts at: 3091 4414 136
Size: 1323 1525
Fragments arranged by size:
1525 1323 672 631 605 402 345 122
107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 2573 3220 2573
Size: 647 5156
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2127 2182 2434 2633 2786 2891 3864
Size: 242 95 55 252 199 153 105 973
Cuts at: 3864 5020 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 973 252 242 199 153 105
95 55
BmgBI CAC'GTC
Cuts at: 1318 2542 1318
Size: 1224 4579
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 2854 3534
Size: 416 53 83 399 24 759 678 680
Cuts at: 3534 4492 442
Size: 958 1753
Fragments arranged by size:
1753 958 759 680 678 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2482 2729 2873 3080 4463 1239
Size: 1243 247 144 207 1383 2579
Fragments arranged by size:
2579 1383 1243 247 207 144
Bpu10I CC'TnA_GC
Cuts at: 2745 2745
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2188 3594 493
Size: 711 969 15 1406 2702
Fragments arranged by size:
2702 1406 969 711 15
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2639 2646 2681 3610
Size: 53 83 186 936 1106 7 35 929
Cuts at: 3610 3992 4627 275
Size: 382 635 1451
Fragments arranged by size:
1451 1106 936 929 635 382 186 83
53 35 7
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2468 2486 2498 1178
Size: 30 165 30 1053 12 18 12 4483
Fragments arranged by size:
4483 1053 165 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 4824
BseYI C'CCAG_C
Cuts at: 2025 2703 5006 5502 2025
Size: 678 2303 496 2326
Fragments arranged by size:
2326 2303 678 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2378 2633 2699 3068 3076 1163
Size: 573 630 12 255 66 369 8 3890
Fragments arranged by size:
3890 630 573 369 255 66 12 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2746 3864 3949 5020 2746
Size: 1118 85 1071 3529
Fragments arranged by size:
3529 1118 1071 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2127 2182 2434 2633 2703 2746
Size: 399 242 95 55 252 199 70 43
Cuts at: 2746 2786 2859 2891 3122 3864 3949 5020 1391
Size: 40 73 32 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 252 242 231 199
95 85 73 70 55 43 40 32
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 2304 2826 2900 3158 844
Size: 1412 48 522 74 258 3489
Fragments arranged by size:
3489 1412 522 258 74 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 2523 3690 1394
Size: 429 397 303 1167 3507
Fragments arranged by size:
3507 1167 429 397 303
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2261 2374 2577 2895 4447 4634 1700
Size: 110 451 113 203 318 1552 187 2869
Fragments arranged by size:
2869 1552 451 318 203 187 113 110
BsrGI T'GTAC_A
Cuts at: 96 2808 96
Size: 2712 3091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 2516 3080 3863 4875 2516
Size: 564 783 1012 3444
Fragments arranged by size:
3444 1012 783 564
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 2353 2593 2790 794
Size: 1529 30 240 197 3807
Fragments arranged by size:
3807 1529 240 197 30
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 2437 1184
Size: 1253 4550
BstYI r'GATC_y
Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798
Size: 1161 367 385 17 1452 86 12 346
Cuts at: 5798 1972
Size: 1977
Fragments arranged by size:
1977 1452 1161 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2209 2224 513
Size: 552 1144 15 4092
Fragments arranged by size:
4092 1144 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2938 487
Size: 683 56 1712 3352
Fragments arranged by size:
3352 1712 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 4113 4133 3319
Size: 794 20 4989
Fragments arranged by size:
4989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2259 2927
Size: 54 1425 50 275 18 219 210 668
Cuts at: 2927 3250 4139 5768 8
Size: 323 889 1629 43
Fragments arranged by size:
1629 1425 889 668 323 275 219 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 3733 1353
Size: 1016 1364 3423
Fragments arranged by size:
3423 1364 1016
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2744 2744
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2482 2729 2784 2873 3080 3866 4463
Size: 393 850 247 55 89 207 786 597
Cuts at: 4463 5250 5518 1239
Size: 787 268 1524
Fragments arranged by size:
1524 850 787 786 597 393 268 247
207 89 55
EcoO109I rG'GnC_Cy
Cuts at: 2178 2911 2939 3015 2178
Size: 733 28 76 4966
Fragments arranged by size:
4966 733 76 28
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 2427 2649 2684 4950 1678
Size: 749 222 35 2266 2531
Fragments arranged by size:
2531 2266 749 222 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 2608 2640 2937
Size: 32 135 32 884 32 120 32 297
Cuts at: 2937 2969 4444 4476 4518 4550 1373
Size: 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 884 297 135 120 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KasI G'GCGC_C
Cuts at: 2645 2680 2645
Size: 35 5768
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 2499 4917 5101 1305
Size: 954 240 2418 184 2007
Fragments arranged by size:
2418 2007 954 240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 10
Size: 54 1425 343 219 3762
Fragments arranged by size:
3762 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 3044 3761 4120 4279 518
Size: 648 1005 873 717 359 159 2042
Fragments arranged by size:
2042 1005 873 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2226 2320 2572 2593 2635 2707 2790
Size: 501 119 94 252 21 42 72 83
Cuts at: 2790 3060 3219 3896 5044 5289 1606
Size: 270 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 270 252 245 159
119 94 83 72 42 21
NaeI GCC'GGC
Cuts at: 2263 2579 2897 2263
Size: 316 318 5169
Fragments arranged by size:
5169 318 316
NarI GG'CG_CC
Cuts at: 2646 2681 2646
Size: 35 5768
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NgoMIV G'CCGG_C
Cuts at: 2261 2577 2895 2261
Size: 316 318 5169
Fragments arranged by size:
5169 318 316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 3161 4706 1132
Size: 97 1932 1545 2229
Fragments arranged by size:
2229 1932 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 2656 1108
Size: 1080 468 4255
Fragments arranged by size:
4255 1080 468
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PmlI CAC'GTG
Cuts at: 2173 2173
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2178 2430 2629 2178
Size: 252 199 5352
Fragments arranged by size:
5352 252 199
PstI C_TGCA'G
Cuts at: 830 2112 2202 2823 3155 830
Size: 1282 90 621 332 3478
Fragments arranged by size:
3478 1282 621 332 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2107 2572 2707 3060 2107
Size: 465 135 353 4850
Fragments arranged by size:
4850 465 353 135
SacI G_AGCT'C
Cuts at: 2746 2746
Size: 5803
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SapI GCTCTTCn'nnn_
Cuts at: 2369 2369
Size: 5803
SbfI CC_TGCA'GG
Cuts at: 2823 3155 2823
Size: 332 5471
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2108 2198 2600 2819 3151 4286 4967
Size: 219 1063 90 402 219 332 1135 681
Cuts at: 4967 5158 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1063 681 402 332 219 219
191 90
SfoI GGC'GCC
Cuts at: 2647 2682 2647
Size: 35 5768
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 2059 2429 2435 2862 3130 1872
Size: 187 370 6 427 268 4545
Fragments arranged by size:
4545 427 370 268 187 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SrfI GCCC'GGGC
Cuts at: 2429 2429
Size: 5803
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 2534 1517
Size: 1017 4786
StyI C'CwwG_G
Cuts at: 513 1065 2382 2728 3086 3242 513
Size: 552 1317 346 358 156 3074
Fragments arranged by size:
3074 1317 552 358 346 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2183 2561 2792 2836 3830 3847
Size: 1255 446 157 378 231 44 994 17
Cuts at: 3847 4000 4185 4630 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 994 446 445 378 231 185
157 153 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 4049 5761
Size: 274 80 33 51 1443 831 1241 1712
Cuts at: 5761 96
Size: 138
Fragments arranged by size:
1712 1443 1241 831 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 3019 2338
Size: 681 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 2057 2427 2433 2860 3128 1870
Size: 187 370 6 427 268 4545
Fragments arranged by size:
4545 427 370 268 187 6
XmnI GAAnn'nnTTC
Cuts at: 2534 3932 2534
Size: 1398 4405
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI
RsrII SanDI SexAI SfiI SwaI