Restriction map for
pFN21AE1120
(Circular) (Six-base) MAPSORT of: pFN21AE1120.seq Check: 6300 from: 1 to: 5797
pFN21AE1120 5797 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2690 2690
Size: 5797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 3860 5244 5512 1632
Size: 989 1239 1384 268 1917
Fragments arranged by size:
1917 1384 1239 989 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2622 4696 5673 1203
Size: 1419 2074 977 1327
Fragments arranged by size:
2074 1419 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2726 1184
Size: 1542 4255
AlwNI CAG_nnn'CTG
Cuts at: 2178 2297 2586 3540 5112 2178
Size: 119 289 954 1572 2863
Fragments arranged by size:
2863 1572 954 289 119
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 3122 1870
Size: 73 702 477 4545
Fragments arranged by size:
4545 702 477 73
BamHI G'GATC_C
Cuts at: 2274 3127 3494 2274
Size: 853 367 4577
Fragments arranged by size:
4577 853 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3116 1391
Size: 1725 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2609 928
Size: 971 710 4116
Fragments arranged by size:
4116 971 710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2507 2924 4408
Size: 107 122 71 631 605 835 417 1484
Cuts at: 4408 136
Size: 1525
Fragments arranged by size:
1525 1484 835 631 605 417 122 107
71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 3858 5014 1790
Size: 2068 1156 2573
Fragments arranged by size:
2573 2068 1156
BmgBI CAC'GTC
Cuts at: 1318 2623 1318
Size: 1305 4492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3528 4486
Size: 416 53 83 399 24 651 1460 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1460 958 651 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 3031 4457 1239
Size: 1169 623 1426 2579
Fragments arranged by size:
2579 1426 1169 623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 2429 2436 3073 4460 882
Size: 617 804 126 7 637 1387 2219
Fragments arranged by size:
2219 1387 804 637 617 126 7
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2387 2417 1178
Size: 30 165 30 687 30 267 30 4558
Fragments arranged by size:
4558 687 267 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5797
BseYI C'CCAG_C
Cuts at: 2010 2365 2844 5000 5496 2010
Size: 355 479 2156 496 2311
Fragments arranged by size:
2311 2156 496 479 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2672 1163
Size: 573 936 4288
Fragments arranged by size:
4288 936 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 3858 3943 5014 2715
Size: 1143 85 1071 3498
Fragments arranged by size:
3498 1143 1071 85
BsmI GAATG_Cn'
Cuts at: 2831 3308 3401 2831
Size: 477 93 5227
Fragments arranged by size:
5227 477 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2407 2407
Size: 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2715 3116 3858 3943 5014 1391
Size: 399 925 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 925 742 401 399 85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2571 2690 3152 844
Size: 1727 119 462 3489
Fragments arranged by size:
3489 1727 462 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2747 3684 1394
Size: 429 924 937 3507
Fragments arranged by size:
3507 937 924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 2491 2520 4292 4466 65
Size: 1183 948 295 29 1772 174 1396
Fragments arranged by size:
1772 1396 1183 948 295 174 29
BsrFI r'CCGG_y
Cuts at: 1700 1810 2923 2982 4441 4628 1700
Size: 110 1113 59 1459 187 2869
Fragments arranged by size:
2869 1459 1113 187 110 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2873 794
Size: 2079 3718
BstBI TT'CG_AA
Cuts at: 2124 2992 4601 2124
Size: 868 1609 3320
Fragments arranged by size:
3320 1609 868
BstEII G'GTnAC_C
Cuts at: 1184 2677 1184
Size: 1493 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 2351 2351
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2274 2535 3127 3494 3879 3896 5348 5434
Size: 302 261 592 367 385 17 1452 86
Cuts at: 5434 5446 5792 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 592 385 367 346 302 261
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2593 513
Size: 552 939 589 3717
Fragments arranged by size:
3717 939 589 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 2302 487
Size: 683 56 902 174 3982
Fragments arranged by size:
3982 902 683 174 56
BtsI GCAGTG_nn'
Cuts at: 2021 3313 4107 4127 2021
Size: 1292 794 20 3691
Fragments arranged by size:
3691 1292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2436 2508
Size: 54 1425 50 275 18 402 204 72
Cuts at: 2508 2736 3244 4133 5762 8
Size: 228 508 889 1629 43
Fragments arranged by size:
1629 1425 889 508 402 275 228 204
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2621 3031 3860 4457 5244 5512
Size: 393 776 213 410 829 597 787 268
Cuts at: 5512 1239
Size: 1524
Fragments arranged by size:
1524 829 787 776 597 410 393 268
213
EcoO109I rG'GnC_Cy
Cuts at: 2117 2117
Size: 5797
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 3054 4944 1678
Size: 1376 1890 2531
Fragments arranged by size:
2531 1890 1376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 2759 2791 3020
Size: 32 135 32 835 32 320 32 229
Cuts at: 3020 3052 3056 3088 4438 4470 4512 4544 1373
Size: 32 4 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 835 320 229 135 42 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3028 3141 3387 669
Size: 988 281 1090 113 246 3079
Fragments arranged by size:
3079 1090 988 281 246 113
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2555 4911 5095 1305
Size: 1250 2356 184 2007
Fragments arranged by size:
2356 2007 1250 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 2510 2738 10
Size: 54 1425 343 606 72 228 3069
Fragments arranged by size:
3069 1425 606 343 228 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 2813 3755 4114 4273 518
Size: 648 1542 105 942 359 159 2042
Fragments arranged by size:
2042 1542 942 648 359 159 105
MspA1I CmG'CkG
Cuts at: 1606 2266 2631 3213 3890 5038 5283 1606
Size: 660 365 582 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 582 365 245
NaeI GCC'GGC
Cuts at: 2925 2925
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4306
Fragments arranged by size:
4306 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NgoMIV G'CCGG_C
Cuts at: 2923 2923
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2871 3155 4700 1132
Size: 97 1642 284 1545 2229
Fragments arranged by size:
2229 1642 1545 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2178 1108
Size: 1070 4727
PfoI T'CCnGG_A
Cuts at: 2385 2531 2385
Size: 146 5651
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3097 3149 830
Size: 2267 52 3478
Fragments arranged by size:
3478 2267 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SexAI A'CCwGG_T
Cuts at: 2343 2343
Size: 5797
SfcI C'TryA_G
Cuts at: 826 1045 3093 3145 4280 4961 5152 826
Size: 219 2048 52 1135 681 191 1471
Fragments arranged by size:
2048 1471 1135 681 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 2647 3124 1872
Size: 775 477 4545
Fragments arranged by size:
4545 775 477
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 2871 3155 1132
Size: 1739 284 3774
Fragments arranged by size:
3774 1739 284
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2004 3236 513
Size: 552 939 1232 3074
Fragments arranged by size:
3074 1232 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2146 2795 3824 3841 3994 4179
Size: 1255 507 59 649 1029 17 153 185
Cuts at: 4179 4624 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1029 649 507 445 185 153
59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 2746
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 2645 3122 1870
Size: 775 477 4545
Fragments arranged by size:
4545 775 477
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI