Restriction map for
pFN21AE1103
(Circular) (Six-base) MAPSORT of: pFN21AE1103.seq Check: 5638 from: 1 to: 5116
pFN21AE1103 5116 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2926 278
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2459 1937
Size: 522 4594
Acc65I G'GTAC_C
Cuts at: 2437 2437
Size: 5116
AclI AA'CG_TT
Cuts at: 1563 3243 3616 1563
Size: 1680 373 3063
Fragments arranged by size:
3063 1680 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2156 2348 3179 4563 4831 1632
Size: 446 78 192 831 1384 268 1917
Fragments arranged by size:
1917 1384 831 446 268 192 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4919
AflIII A'CryG_T
Cuts at: 1203 4015 4992 1203
Size: 2812 977 1327
Fragments arranged by size:
2812 1327 977
AgeI A'CCGG_T
Cuts at: 3947 3947
Size: 5116
AhdI GACnn_n'nnGTC
Cuts at: 3845 3845
Size: 5116
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5116
AlwNI CAG_nnn'CTG
Cuts at: 2859 4431 2859
Size: 1572 3544
ApaLI G'TGCA_C
Cuts at: 3173 4329 3173
Size: 1156 3960
ApoI r'AATT_y
Cuts at: 1493 2425 2651 2803 4912 1493
Size: 932 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3670 160
Size: 872 2638 1606
Fragments arranged by size:
2638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2441 1870
Size: 73 202 296 4545
Fragments arranged by size:
4545 296 202 73
BamHI G'GATC_C
Cuts at: 2446 2813 2446
Size: 367 4749
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2437 3892 618
Size: 325 217 627 525 125 1455 1842
Fragments arranged by size:
1842 1455 627 525 325 217 125
BanII G_rGCy'C
Cuts at: 1391 2399 2435 1391
Size: 1008 36 4072
Fragments arranged by size:
4072 1008 36
BbeI G_GCGC'C
Cuts at: 2316 2316
Size: 5116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 928
Size: 971 306 3839
Fragments arranged by size:
3839 971 306
BbvCI CC'TCA_GC
Cuts at: 2111 2111
Size: 5116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3307 3341 1903
Size: 34 1370 34 3678
Fragments arranged by size:
3678 1370 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3008 4218 3008
Size: 1210 3906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3727 136
Size: 107 122 71 631 605 2055 1525
Fragments arranged by size:
2055 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5111 5111
Size: 5116
BlpI GC'TnA_GC
Cuts at: 2533 2533
Size: 5116
Bme1580I G_kGCm'C
Cuts at: 1790 3177 4333 1790
Size: 1387 1156 2573
Fragments arranged by size:
2573 1387 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2847 3805 442
Size: 416 53 83 399 24 1430 958 1753
Fragments arranged by size:
1753 1430 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2316 2316
Size: 5116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 3776 1239
Size: 1072 1465 2579
Fragments arranged by size:
2579 1465 1072
Bpu10I CC'TnA_GC
Cuts at: 2111 2393 2111
Size: 282 4834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 3241 4106 4404 2054
Size: 1187 865 298 2766
Fragments arranged by size:
2766 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3779 882
Size: 617 2280 2219
Fragments arranged by size:
2280 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2907 493
Size: 711 1703 2702
Fragments arranged by size:
2702 1703 711
BsaBI GATnn'nnATC
Cuts at: 5110 5110
Size: 5116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 2923 3305 3940
Size: 53 83 186 936 780 610 382 635
Cuts at: 3940 275
Size: 1451
Fragments arranged by size:
1451 936 780 635 610 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2049 3549 3947 4221 4368 4779 2049
Size: 1500 398 274 147 411 2386
Fragments arranged by size:
2386 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4891
Fragments arranged by size:
4891 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 4182
BseYI C'CCAG_C
Cuts at: 2305 4319 4815 2305
Size: 2014 496 2606
Fragments arranged by size:
2606 2014 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2566 3327 4355 1540
Size: 275 186 565 761 1028 2301
Fragments arranged by size:
2301 1028 761 565 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2399 3177 3262 4333 2091
Size: 308 778 85 1071 2874
Fragments arranged by size:
2874 1071 778 308 85
BsmI GAATG_Cn'
Cuts at: 2627 2720 2627
Size: 93 5023
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2237
Size: 5116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2399 2435 3177 3262 4333 1391
Size: 399 301 308 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 301 85 36
BspEI T'CCGG_A
Cuts at: 4779 4779
Size: 5116
BspHI T'CATG_A
Cuts at: 3005 4735 3005
Size: 1730 3386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2471 844
Size: 1627 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 1394
Size: 429 1180 3507
Fragments arranged by size:
3507 1180 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3611 3785 65
Size: 1183 2363 174 1396
Fragments arranged by size:
2363 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2275 3760 3947 1700
Size: 110 204 261 1485 187 2869
Fragments arranged by size:
2869 1485 261 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5116
BssSI C'ACGA_G
Cuts at: 2090 3176 4188 2090
Size: 1086 1012 3018
Fragments arranged by size:
3018 1086 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5116
BstBI TT'CG_AA
Cuts at: 3920 3920
Size: 5116
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5116
BstYI r'GATC_y
Cuts at: 1972 2446 2813 3198 3215 4667 4753 4765 5111
Size: 474 367 385 17 1452 86 12 346
Cuts at: 5111 1972
Size: 1977
Fragments arranged by size:
1977 1452 474 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4564
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4377
Fragments arranged by size:
4377 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 2632 3426 3446 2220
Size: 412 794 20 3890
Fragments arranged by size:
3890 794 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5081
DraI TTT'AAA
Cuts at: 2420 2420
Size: 5116
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4123 809
Size: 507 2807 1802
Fragments arranged by size:
2807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2563 3452 5081
Size: 54 1425 50 275 18 733 889 1629
Cuts at: 5081 8
Size: 43
Fragments arranged by size:
1629 1425 889 733 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2563 1537
Size: 275 751 4090
Fragments arranged by size:
4090 751 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2209 2285 3046 1353
Size: 856 76 761 3423
Fragments arranged by size:
3423 856 761 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 3703 4077 4223 387
Size: 1064 138 444 1670 374 146 1280
Fragments arranged by size:
1670 1280 1064 444 374 146 138
EcoICRI GAG'CTC
Cuts at: 2397 2397
Size: 5116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2156 2311 2348 3179 3776 4563
Size: 393 446 78 155 37 831 597 787
Cuts at: 4563 4831 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 597 446 393 268 155
78 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2349 2349
Size: 5116
EcoO109I rG'GnC_Cy
Cuts at: 2377 2377
Size: 5116
EcoRI G'AATT_C
Cuts at: 2425 2803 2425
Size: 378 4738
FspI TGC'GCA
Cuts at: 3622 3622
Size: 5116
HaeII r_GCGC'y
Cuts at: 1678 2316 4263 1678
Size: 638 1947 2531
Fragments arranged by size:
2531 1947 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 3757 3789 3831
Size: 32 135 32 614 32 1539 32 42
Cuts at: 3831 3863 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 614 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2460 2706 669
Size: 988 281 522 246 3079
Fragments arranged by size:
3079 988 522 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5116
HpaI GTT'AAC
Cuts at: 2706 2706
Size: 5116
KasI G'GCGC_C
Cuts at: 2312 2312
Size: 5116
KpnI G_GTAC'C
Cuts at: 2441 2441
Size: 5116
MfeI C'AATT_G
Cuts at: 2715 2715
Size: 5116
MluI A'CGCG_T
Cuts at: 4992 4992
Size: 5116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4230 4414 1305
Size: 2925 184 2007
Fragments arranged by size:
2925 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3294
Fragments arranged by size:
3294 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 3433 3592 518
Size: 648 1908 359 159 2042
Fragments arranged by size:
2042 1908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2532 3209 4357 4602 1606
Size: 699 227 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 245 227
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 5116
NarI GG'CG_CC
Cuts at: 2313 2313
Size: 5116
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4564
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5116
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 5116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5116
NotI GC'GGCC_GC
Cuts at: 2563 2563
Size: 5116
NspI r_CATG'y
Cuts at: 1132 1229 2474 4019 1132
Size: 97 1245 1545 2229
Fragments arranged by size:
2229 1545 1245 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5116
PciI A'CATG_T
Cuts at: 4015 4015
Size: 5116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5116
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 5116
PmeI GTTT'AAAC
Cuts at: 2420 2420
Size: 5116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 4698 4730 3185
Size: 32 1481 32 3571
Fragments arranged by size:
3571 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2377
Size: 5116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5116
PsiI TTA'TAA
Cuts at: 2686 2686
Size: 5116
PstI C_TGCA'G
Cuts at: 830 2181 2468 830
Size: 1351 287 3478
Fragments arranged by size:
3478 1351 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5116
SacI G_AGCT'C
Cuts at: 2399 2399
Size: 5116
SalI G'TCGA_C
Cuts at: 1936 2458 1936
Size: 522 4594
SanDI GG'GwC_CC
Cuts at: 2377 2377
Size: 5116
SbfI CC_TGCA'GG
Cuts at: 2468 2468
Size: 5116
ScaI AGT'ACT
Cuts at: 3364 5076 3364
Size: 1712 3404
SfcI C'TryA_G
Cuts at: 826 1045 2177 2464 3599 4280 4471 826
Size: 219 1132 287 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 287 219 191
SfoI GGC'GCC
Cuts at: 2314 2314
Size: 5116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5116
SmaI CCC'GGG
Cuts at: 1872 2147 2443 1872
Size: 275 296 4545
Fragments arranged by size:
4545 296 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 3220 4121 4383 820
Size: 197 926 126 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 262 197 126
SnaBI TAC'GTA
Cuts at: 493 2907 493
Size: 2414 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5116
SphI G_CATG'C
Cuts at: 1132 2474 1132
Size: 1342 3774
SspI AAT'ATT
Cuts at: 5 52 3040 5
Size: 47 2988 2081
Fragments arranged by size:
2988 2081 47
StuI AGG'CCT
Cuts at: 1517 2369 1517
Size: 852 4264
StyI C'CwwG_G
Cuts at: 513 1065 2555 513
Size: 552 1490 3074
Fragments arranged by size:
3074 1490 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2184 2272 3143 3160 3313 3498 3943
Size: 1255 604 88 871 17 153 185 445
Cuts at: 3943 325
Size: 1498
Fragments arranged by size:
1498 1255 871 604 445 185 153 88
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 5074 96
Size: 274 80 33 51 1443 1385 1712 138
Fragments arranged by size:
1712 1443 1385 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5076 5108 5076
Size: 32 5084
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5116
XbaI T'CTAG_A
Cuts at: 2452 2452
Size: 5116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5116
XmaI C'CCGG_G
Cuts at: 1870 2145 2441 1870
Size: 275 296 4545
Fragments arranged by size:
4545 296 275
XmnI GAAnn'nnTTC
Cuts at: 3245 3245
Size: 5116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2924 276
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI
XcmI