Restriction map for
pFN21AE1100
(Circular) (Six-base) MAPSORT of: pFN21AE1100.seq Check: 9488 from: 1 to: 5620
pFN21AE1100 5620 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 2931 3683 5067 5335 1632
Size: 486 813 752 1384 268 1917
Fragments arranged by size:
1917 1384 813 752 486 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 5588
AlwNI CAG_nnn'CTG
Cuts at: 2153 3363 4935 2153
Size: 1210 1572 2838
Fragments arranged by size:
2838 1572 1210
ApaLI G'TGCA_C
Cuts at: 2683 3677 4833 2683
Size: 994 1156 3470
Fragments arranged by size:
3470 1156 994
ApoI r'AATT_y
Cuts at: 1493 2773 2929 3155 3307 5416 1493
Size: 1280 156 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2014 2950 3317 2014
Size: 936 367 4317
Fragments arranged by size:
4317 936 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 2274 2300 2429 2919 928
Size: 971 219 156 26 129 490 3629
Fragments arranged by size:
3629 971 490 219 156 129 26
BbvCI CC'TCA_GC
Cuts at: 2363 2405 2512 2363
Size: 42 107 5471
Fragments arranged by size:
5471 107 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2687 3681 4837 1790
Size: 897 994 1156 2573
Fragments arranged by size:
2573 1156 994 897
BmgBI CAC'GTC
Cuts at: 1318 2917 1318
Size: 1599 4021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2583 3351 4309
Size: 416 53 83 399 24 1166 768 958
Cuts at: 4309 442
Size: 1753
Fragments arranged by size:
1753 1166 958 768 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2641 2671 4280 1239
Size: 1115 287 30 1609 2579
Fragments arranged by size:
2579 1609 1115 287 30
Bpu10I CC'TnA_GC
Cuts at: 2363 2405 2512 2363
Size: 42 107 5471
Fragments arranged by size:
5471 107 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2143 2173 1178
Size: 30 165 30 740 30 4625
Fragments arranged by size:
4625 740 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 1186
Size: 965 4655
BseYI C'CCAG_C
Cuts at: 2319 2528 2813 4823 5319 2319
Size: 209 285 2010 496 2620
Fragments arranged by size:
2620 2010 496 285 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2687 3681 3766 4837 2687
Size: 994 85 1071 3470
Fragments arranged by size:
3470 1071 994 85
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 2939 3681 3766 4837 1391
Size: 399 897 252 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 252 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2497 4115 4289 65
Size: 1183 1249 1618 174 1396
Fragments arranged by size:
1618 1396 1249 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2791 2011
Size: 780 4840
BstYI r'GATC_y
Cuts at: 1972 2014 2179 2950 3317 3702 3719 5171 5257
Size: 42 165 771 367 385 17 1452 86
Cuts at: 5257 5269 5615 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 771 385 367 346 165 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2871 513
Size: 552 1110 696 3262
Fragments arranged by size:
3262 1110 696 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2477 3067 3956
Size: 54 1425 50 275 18 647 590 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 647 590 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2317 2545 3550 1353
Size: 816 148 228 1005 3423
Fragments arranged by size:
3423 1005 816 228 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2354 2641 2671 2931 3683 4280
Size: 393 486 236 287 30 260 752 597
Cuts at: 4280 5067 5335 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 752 597 486 393 287 268
260 236 30
EcoO109I rG'GnC_Cy
Cuts at: 2275 2275
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2055 2055
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2143 2175 4261
Size: 32 135 32 427 32 112 32 2086
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2086 427 135 112 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2032 748
Size: 1284 4336
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2415 4734 4918 1305
Size: 774 336 2319 184 2007
Fragments arranged by size:
2319 2007 774 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2900 3578 3937 4096 518
Size: 648 843 891 678 359 159 2042
Fragments arranged by size:
2042 891 843 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2323 3036 3713 4861 5106 1606
Size: 717 713 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 713 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2175 2871 513
Size: 552 1110 696 3262
Fragments arranged by size:
3262 1110 696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2761 2905 2978 4523 1132
Size: 97 1532 144 73 1545 2229
Fragments arranged by size:
2229 1545 1532 144 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PfoI T'CCnGG_A
Cuts at: 2648 2663 2693 2648
Size: 15 30 5575
Fragments arranged by size:
5575 30 15
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5620
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2393 2425 2393
Size: 32 5588
PstI C_TGCA'G
Cuts at: 830 2245 2751 2972 830
Size: 1415 506 221 3478
Fragments arranged by size:
3478 1415 506 221
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2323 2323
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 2860 3868 5580 2860
Size: 1008 1712 2900
Fragments arranged by size:
2900 1712 1008
SexAI A'CCwGG_T
Cuts at: 2426 2678 2426
Size: 252 5368
SfcI C'TryA_G
Cuts at: 826 1045 2241 2747 2968 4103 4784 4975 826
Size: 219 1196 506 221 1135 681 191 1471
Fragments arranged by size:
1471 1196 1135 681 506 221 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2761 2978 1132
Size: 1629 217 3774
Fragments arranged by size:
3774 1629 217
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2510 2708 2823 1517
Size: 993 198 115 4314
Fragments arranged by size:
4314 993 198 115
StyI C'CwwG_G
Cuts at: 513 1065 2175 2802 2871 3059 513
Size: 552 1110 627 69 188 3074
Fragments arranged by size:
3074 1110 627 552 188 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 2702 3647 3664 3817 4002 4447
Size: 1255 753 369 945 17 153 185 445
Cuts at: 4447 325
Size: 1498
Fragments arranged by size:
1498 1255 945 753 445 369 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 2858 3866
Size: 274 80 33 51 1443 352 529 1008
Cuts at: 3866 5578 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1008 529 352 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2547 2579 5580 5612 2547
Size: 32 3001 32 2555
Fragments arranged by size:
3001 2555 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2273 1316
Size: 957 4663
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI