Restriction map for
pFN21AE1096
(Circular) (Six-base) MAPSORT of: pFN21AE1096.seq Check: 8263 from: 1 to: 5803
pFN21AE1096 5803 bp
With 182 enzymes: SgfI *
November 19, 2009 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2159 2159
Size: 5803
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3866 5250 5518 1632
Size: 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 268
AfeI AGC'GCT
Cuts at: 2412 2426 2412
Size: 14 5789
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 2153 1166
Size: 987 4816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 3546 5118 3546
Size: 1572 4231
ApaI G_GGCC'C
Cuts at: 2535 2535
Size: 5803
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3096 4357 160
Size: 872 2064 1261 1606
Fragments arranged by size:
2064 1606 1261 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 3072 3128 1870
Size: 73 1129 56 4545
Fragments arranged by size:
4545 1129 73 56
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2570 2753 3124 4579 618
Size: 325 217 627 783 183 371 1455 1842
Fragments arranged by size:
1842 1455 783 627 371 325 217 183
BanII G_rGCy'C
Cuts at: 1391 2535 3122 1391
Size: 1144 587 4072
Fragments arranged by size:
4072 1144 587
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2671 928
Size: 971 772 4060
Fragments arranged by size:
4060 971 772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 2414 3220 2414
Size: 806 4997
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3864 5020 1790
Size: 745 1329 1156 2573
Fragments arranged by size:
2573 1329 1156 745
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2074 3534 4492
Size: 416 53 83 399 24 657 1460 958
Cuts at: 4492 442
Size: 1753
Fragments arranged by size:
1753 1460 958 657 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4463 1239
Size: 3224 2579
Bpu10I CC'TnA_GC
Cuts at: 2762 2762
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3594 493
Size: 711 1641 749 2702
Fragments arranged by size:
2702 1641 749 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2139 3610 3992
Size: 53 83 186 936 485 121 1471 382
Cuts at: 3992 4627 275
Size: 635 1451
Fragments arranged by size:
1471 1451 936 635 485 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5578
Fragments arranged by size:
5578 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2743 1186
Size: 1150 407 4246
Fragments arranged by size:
4246 1150 407
BseYI C'CCAG_C
Cuts at: 2021 2092 5006 5502 2021
Size: 71 2914 496 2322
Fragments arranged by size:
2914 2322 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 1163
Size: 573 629 4601
Fragments arranged by size:
4601 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2562 3864 3949 5020 2400
Size: 162 1302 85 1071 3183
Fragments arranged by size:
3183 1302 1071 162 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2535 2562 3122 3864 3949 5020
Size: 399 610 135 27 560 742 85 1071
Cuts at: 5020 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 610 560 399 135 85
27
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 2196 3158 844
Size: 1315 37 962 3489
Fragments arranged by size:
3489 1315 962 37
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2322 4447 4634 1700
Size: 110 512 2125 187 2869
Fragments arranged by size:
2869 2125 512 187 110
BsrGI T'GTAC_A
Cuts at: 96 2970 96
Size: 2874 2929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 2561 2921 3863 4875 2561
Size: 360 942 1012 3489
Fragments arranged by size:
3489 1012 942 360
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 3024 4607 3024
Size: 1583 4220
BstEII G'GTnAC_C
Cuts at: 1184 2467 1184
Size: 1283 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2203 2464 2155
Size: 48 261 5494
Fragments arranged by size:
5494 261 48
BstYI r'GATC_y
Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798
Size: 1161 367 385 17 1452 86 12 346
Cuts at: 5798 1972
Size: 1977
Fragments arranged by size:
1977 1452 1161 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2391 2511 513
Size: 552 1326 120 3805
Fragments arranged by size:
3805 1326 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 2754 487
Size: 683 56 935 593 3536
Fragments arranged by size:
3536 935 683 593 56
BtsI GCAGTG_nn'
Cuts at: 3009 3319 4113 4133 3009
Size: 310 794 20 4679
Fragments arranged by size:
4679 794 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2593 2628 529
Size: 35 2029 35 3704
Fragments arranged by size:
3704 2029 35 35
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 3250 4139
Size: 54 1425 50 275 18 222 1198 889
Cuts at: 4139 5768 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1198 889 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2120 2168 2363 2942 3733 1353
Size: 669 98 48 195 579 791 3423
Fragments arranged by size:
3423 791 669 579 195 98 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2740 4390 4764 4910 387
Size: 1064 138 1151 1650 374 146 1280
Fragments arranged by size:
1650 1280 1151 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3866 4463 5250 5518 1239
Size: 393 2234 597 787 268 1524
Fragments arranged by size:
2234 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2761 2761
Size: 5803
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 2228 4309 2228
Size: 2081 3722
HaeII r_GCGC'y
Cuts at: 1678 2414 2428 2529 4950 1678
Size: 736 14 101 2421 2531
Fragments arranged by size:
2531 2421 736 101 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373
Size: 32 135 32 2872 32 42 32 2626
Fragments arranged by size:
2872 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2201 2540 2639 3761 4120 4279
Size: 648 987 48 339 99 1122 359 159
Cuts at: 4279 518
Size: 2042
Fragments arranged by size:
2042 1122 987 648 359 339 159 99
48
MspA1I CmG'CkG
Cuts at: 1606 2128 3219 3896 5044 5289 1606
Size: 522 1091 677 1148 245 2120
Fragments arranged by size:
2120 1148 1091 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2293 3161 4706 1132
Size: 97 1064 868 1545 2229
Fragments arranged by size:
2229 1545 1064 868 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 3872 3904 5385 5417 2021
Size: 32 1819 32 1481 32 2407
Fragments arranged by size:
2407 1819 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2531 2531
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2112 2525 3155 830
Size: 1282 413 630 3478
Fragments arranged by size:
3478 1282 630 413
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SapI GCTCTTCn'nnn_
Cuts at: 2022 2120 2022
Size: 98 5705
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 2912 4051 5763 2912
Size: 1139 1712 2952
Fragments arranged by size:
2952 1712 1139
SfcI C'TryA_G
Cuts at: 826 1045 2108 2521 2698 2802 3151 4286 4967
Size: 219 1063 413 177 104 349 1135 681
Cuts at: 4967 5158 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1063 681 413 349 219 191
177 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 2293 3161 1132
Size: 1161 868 3774
Fragments arranged by size:
3774 1161 868
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2457 2472 2579 2886 3242 513
Size: 552 1392 15 107 307 356 3074
Fragments arranged by size:
3074 1392 552 356 307 107 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2638 2741 3830 3847 4000 4185 4630
Size: 1255 1058 103 1089 17 153 185 445
Cuts at: 4630 325
Size: 1498
Fragments arranged by size:
1498 1255 1089 1058 445 185 153 103
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2910 2970 4049
Size: 274 80 33 51 1443 933 60 1079
Cuts at: 4049 5761 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1079 933 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI