Restriction map for
pFN21AE1095
(Circular) (Six-base) MAPSORT of: pFN21AE1095.seq Check: 7041 from: 1 to: 5785
pFN21AE1095 5785 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 2134
Size: 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 2645 3106 2645
Size: 461 5324
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2722 3848 5232 5500 1632
Size: 749 341 1126 1384 268 1917
Fragments arranged by size:
1917 1384 1126 749 341 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 3051 4684 5661 1203
Size: 1848 1633 977 1327
Fragments arranged by size:
1848 1633 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 2499 2621 1166
Size: 1333 122 4330
Fragments arranged by size:
4330 1333 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2618 3528 5100 2618
Size: 910 1572 3303
Fragments arranged by size:
3303 1572 910
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 3094 3320 3472 5581 1493
Size: 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2424 2488 3110 1870
Size: 73 316 165 64 622 4545
Fragments arranged by size:
4545 622 316 165 73 64
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2645 3106 4561 618
Size: 325 217 627 756 102 461 1455 1842
Fragments arranged by size:
1842 1455 756 627 461 325 217 102
BanII G_rGCy'C
Cuts at: 1391 2112 2445 3104 1391
Size: 721 333 659 4072
Fragments arranged by size:
4072 721 659 333
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3004 928
Size: 971 1105 3709
Fragments arranged by size:
3709 1105 971
BbvCI CC'TCA_GC
Cuts at: 2629 2629
Size: 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2800 3677 4887 2800
Size: 877 1210 3698
Fragments arranged by size:
3698 1210 877
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2232 5780 2232
Size: 3548 2237
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 3846 5002 1790
Size: 2056 1156 2573
Fragments arranged by size:
2573 2056 1156
BmgBI CAC'GTC
Cuts at: 1318 3052 1318
Size: 1734 4051
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2486 4445 1239
Size: 1076 171 1959 2579
Fragments arranged by size:
2579 1959 1076 171
Bpu10I CC'TnA_GC
Cuts at: 2629 2629
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3910 4775 5073 2786
Size: 1124 865 298 3498
Fragments arranged by size:
3498 1124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2875 3576 493
Size: 711 1671 701 2702
Fragments arranged by size:
2702 1671 711 701
BsaBI GATnn'nnATC
Cuts at: 2351 5779 2351
Size: 3428 2357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 2810 2840 1178
Size: 30 165 30 1332 30 45 30 4123
Fragments arranged by size:
4123 1332 165 45 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2040 2740 1186
Size: 854 700 4231
Fragments arranged by size:
4231 854 700
BseYI C'CCAG_C
Cuts at: 2459 4988 5484 2459
Size: 2529 496 2760
Fragments arranged by size:
2760 2529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 1163
Size: 573 423 4789
Fragments arranged by size:
4789 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3235 3996 5024 1540
Size: 275 186 943 291 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2534 3846 3931 5002 2534
Size: 1312 85 1071 3317
Fragments arranged by size:
3317 1312 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2445 2534 3104 3846 3931 5002
Size: 399 322 333 89 570 742 85 1071
Cuts at: 5002 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 570 399 333 322 89
85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 3140 844
Size: 1290 1006 3489
Fragments arranged by size:
3489 1290 1006
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 2826 3672 1394
Size: 429 358 645 846 3507
Fragments arranged by size:
3507 846 645 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 2973 4280 4454 65
Size: 1183 1725 1307 174 1396
Fragments arranged by size:
1725 1396 1307 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2138 3845 4857 2138
Size: 1707 1012 3066
Fragments arranged by size:
3066 1707 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2191 2191
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2232 3115 3482 3867 3884 5336 5422 5434
Size: 260 883 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 883 385 367 346 260 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 4222
Fragments arranged by size:
4222 1011 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 4095 4115 3301
Size: 794 20 4971
Fragments arranged by size:
4971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2381 2486 2722 3848 4445 5232
Size: 393 683 66 105 236 1126 597 787
Cuts at: 5232 5500 1239
Size: 268 1524
Fragments arranged by size:
1524 1126 787 683 597 393 268 236
105 66
EcoO109I rG'GnC_Cy
Cuts at: 2855 2994 2855
Size: 139 5646
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 2093 2330 4291 2093
Size: 237 1961 3587
Fragments arranged by size:
3587 1961 237
FspAI rTGC'GCAy
Cuts at: 2093 2330 2093
Size: 237 5548
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2657 2689 4426 4458 4500
Size: 32 135 32 1085 32 1737 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 1737 1085 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 3129 3375 669
Size: 988 281 1027 164 246 3079
Fragments arranged by size:
3079 1027 988 281 246 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 2649 3110 2649
Size: 461 5324
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 4899 5083 1305
Size: 903 2691 184 2007
Fragments arranged by size:
2691 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 2499 2580 2621 3006 3743 4102
Size: 648 1209 124 81 41 385 737 359
Cuts at: 4102 4261 518
Size: 159 2042
Fragments arranged by size:
2042 1209 737 648 385 359 159 124
81 41
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 4222
Fragments arranged by size:
4222 1011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2335 3143 4688 1132
Size: 97 1106 808 1545 2229
Fragments arranged by size:
2229 1545 1106 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 3854 3886 5367 5399 2951
Size: 32 871 32 1481 32 3337
Fragments arranged by size:
3337 1481 871 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2994 2994
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2619 3133 4268 4949 5140 826
Size: 219 1574 514 1135 681 191 1471
Fragments arranged by size:
1574 1471 1135 681 514 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 2261 2426 2490 3112 1872
Size: 389 165 64 622 4545
Fragments arranged by size:
4545 622 389 165 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 3889 4790 5052 820
Size: 197 926 858 1088 901 262 1553
Fragments arranged by size:
1553 1088 926 901 858 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 2335 3143 1132
Size: 1203 808 3774
Fragments arranged by size:
3774 1203 808
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2076 2196 2277 2436 2676 3224 513
Size: 552 1011 120 81 159 240 548 3074
Fragments arranged by size:
3074 1011 552 548 240 159 120 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3812 3829 3982 4167 4612 325
Size: 1255 1350 882 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4031 5743
Size: 274 80 33 51 1443 402 1652 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1652 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2735 2767 2870 2902 5745 5777 2735
Size: 32 103 32 2843 32 2743
Fragments arranged by size:
2843 2743 103 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2683 2284
Size: 399 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 2259 2424 2488 3110 1870
Size: 389 165 64 622 4545
Fragments arranged by size:
4545 622 389 165 64
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI