Restriction map for
pFN21AE1089
(Circular) (Six-base) MAPSORT of: pFN21AE1089.seq Check: 6628 from: 1 to: 6073
pFN21AE1089 6073 bp
With 182 enzymes: SgfI *
October 14, 2009 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 3315 3416 1937
Size: 742 636 101 4594
Fragments arranged by size:
4594 742 636 101
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4136 5520 5788 1632
Size: 2504 1384 268 1917
Fragments arranged by size:
2504 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 3268 4802 3268
Size: 1534 4539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 3816 5388 3816
Size: 1572 4501
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2312 2552 3382 3608 3760 5869 1493
Size: 819 240 830 226 152 2109 1697
Fragments arranged by size:
2109 1697 830 819 240 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 3154 3398 1870
Size: 73 1211 244 4545
Fragments arranged by size:
4545 1211 244 73
BamHI G'GATC_C
Cuts at: 2046 3079 3403 3770 2046
Size: 1033 324 367 4349
Fragments arranged by size:
4349 1033 367 324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2459 3394 4849 618
Size: 325 217 627 672 935 1455 1842
Fragments arranged by size:
1842 1455 935 672 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3392 1391
Size: 2001 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 2216 928
Size: 971 220 97 4785
Fragments arranged by size:
4785 971 220 97
BbvCI CC'TCA_GC
Cuts at: 3345 3345
Size: 6073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BfrBI ATG'CAT
Cuts at: 2509 2787 3032 2509
Size: 278 245 5550
Fragments arranged by size:
5550 278 245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4684 136
Size: 107 122 71 631 605 3012 1525
Fragments arranged by size:
3012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3160 3490 3160
Size: 330 5743
Bme1580I G_kGCm'C
Cuts at: 1790 4134 5290 1790
Size: 2344 1156 2573
Fragments arranged by size:
2573 2344 1156
BmgBI CAC'GTC
Cuts at: 1318 2645 1318
Size: 1327 4746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3328 3804 4762
Size: 416 53 83 399 24 1911 476 958
Cuts at: 4762 442
Size: 1753
Fragments arranged by size:
1911 1753 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4733 1239
Size: 3494 2579
Bpu10I CC'TnA_GC
Cuts at: 3345 3345
Size: 6073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2170 4736 882
Size: 617 671 2566 2219
Fragments arranged by size:
2566 2219 671 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 3864 493
Size: 711 2123 537 2702
Fragments arranged by size:
2702 2123 711 537
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2337 2374 4506 4904 5178 5325 5736 2337
Size: 37 2132 398 274 147 411 2674
Fragments arranged by size:
2674 2132 411 398 274 147 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 2700 2730 3094
Size: 30 165 30 778 30 489 30 364
Cuts at: 3094 3124 1178
Size: 30 4127
Fragments arranged by size:
4127 778 489 364 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6073
BseYI C'CCAG_C
Cuts at: 5276 5772 5276
Size: 496 5577
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2063 2877 4134 4219 5290 2063
Size: 814 1257 85 1071 2846
Fragments arranged by size:
2846 1257 1071 814 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2877 3392 4134 4219 5290 1391
Size: 399 273 814 515 742 85 1071 2174
Fragments arranged by size:
2174 1071 814 742 515 399 273 85
BspEI T'CCGG_A
Cuts at: 2374 5736 2374
Size: 3362 2711
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2787 3428 844
Size: 1943 641 3489
Fragments arranged by size:
3489 1943 641
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3168 4568 4742 65
Size: 1183 1920 1400 174 1396
Fragments arranged by size:
1920 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 3700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 2140
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 2046 3079 3403 3770 4155 4172 5624 5710
Size: 74 1033 324 367 385 17 1452 86
Cuts at: 5710 5722 6068 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1033 385 367 346 324 86
74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2919 2919
Size: 6073
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5521
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 3589 4383 4403 3589
Size: 794 20 5259
Fragments arranged by size:
5259 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 2317 3333 3377 2317
Size: 1016 44 5013
Fragments arranged by size:
5013 1016 44
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3141 3520 4409
Size: 54 1425 50 275 18 1311 379 889
Cuts at: 4409 6038 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1311 889 379 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 4003 1353
Size: 1385 1265 3423
Fragments arranged by size:
3423 1385 1265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4136 4733 5520 5788 1239
Size: 393 2504 597 787 268 1524
Fragments arranged by size:
2504 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2918 2918
Size: 6073
EcoO109I rG'GnC_Cy
Cuts at: 2321 2321
Size: 6073
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
EcoRV GAT'ATC
Cuts at: 2218 2218
Size: 6073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2123 2583 3256 2123
Size: 460 673 4940
Fragments arranged by size:
4940 673 460
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3254 3286 4714
Size: 32 135 32 427 32 1223 32 1428
Cuts at: 4714 4746 4788 4820 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1428 1223 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3137 5187 5371 1305
Size: 1832 2050 184 2007
Fragments arranged by size:
2050 2007 1832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3143 10
Size: 54 1425 343 1311 2940
Fragments arranged by size:
2940 1425 1311 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 4031 4390 4549 518
Size: 648 1257 1608 359 159 2042
Fragments arranged by size:
2042 1608 1257 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3289 3489 4166 5314 5559 1606
Size: 1683 200 677 1148 245 2120
Fragments arranged by size:
2120 1683 1148 677 245 200
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5521
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NsiI A_TGCA'T
Cuts at: 2511 2789 3034 2511
Size: 278 245 5550
Fragments arranged by size:
5550 278 245
NspI r_CATG'y
Cuts at: 1132 1229 3431 4976 1132
Size: 97 2202 1545 2229
Fragments arranged by size:
2229 2202 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 4924
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2321 2321
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 2089 1613
Size: 476 5597
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PstI C_TGCA'G
Cuts at: 830 3425 830
Size: 2595 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 3421 4556 5237 5428 826
Size: 219 2376 1135 681 191 1471
Fragments arranged by size:
2376 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3156 3400 1872
Size: 1284 244 4545
Fragments arranged by size:
4545 1284 244
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4177 5078 5340 820
Size: 197 926 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 3864 493
Size: 2834 537 2702
Fragments arranged by size:
2834 2702 537
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 3512 513
Size: 552 2447 3074
Fragments arranged by size:
3074 2447 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3279 4100 4117 4270 4455 4900 325
Size: 1255 1699 821 17 153 185 445 1498
Fragments arranged by size:
1699 1498 1255 821 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2702 4319
Size: 274 80 33 51 1443 492 233 1617
Cuts at: 4319 6031 96
Size: 1712 138
Fragments arranged by size:
1712 1617 1443 492 274 233 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3094 3126 6033 6065 3094
Size: 32 2907 32 3102
Fragments arranged by size:
3102 2907 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 3154 3398 1870
Size: 1284 244 4545
Fragments arranged by size:
4545 1284 244
XmnI GAAnn'nnTTC
Cuts at: 4202 4202
Size: 6073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI