Restriction map for
pFN21AE1087
(Circular) (Six-base) MAPSORT of: pFN21AE1087.seq Check: 1462 from: 1 to: 8422
pFN21AE1087 8422 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2331 2331
Size: 8422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6232 278
Size: 53 83 186 936 4696 2468
Fragments arranged by size:
4696 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5765 1937
Size: 3828 4594
Acc65I G'GTAC_C
Cuts at: 3720 3914 5743 3720
Size: 194 1829 6399
Fragments arranged by size:
6399 1829 194
AclI AA'CG_TT
Cuts at: 1563 6549 6922 1563
Size: 4986 373 3063
Fragments arranged by size:
4986 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 6485 7869 8137 1632
Size: 2048 2805 1384 268 1917
Fragments arranged by size:
2805 2048 1917 1384 268
AfeI AGC'GCT
Cuts at: 4895 5655 4895
Size: 760 7662
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8225
AflIII A'CryG_T
Cuts at: 1203 3201 7321 8298 1203
Size: 1998 4120 977 1327
Fragments arranged by size:
4120 1998 1327 977
AgeI A'CCGG_T
Cuts at: 5562 7253 5562
Size: 1691 6731
AhdI GACnn_n'nnGTC
Cuts at: 7151 7151
Size: 8422
AleI CACnn'nnGTG
Cuts at: 1166 4661 4817 1166
Size: 3495 156 4771
Fragments arranged by size:
4771 3495 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3207 4902 1184
Size: 2023 1695 4704
Fragments arranged by size:
4704 2023 1695
AlwNI CAG_nnn'CTG
Cuts at: 2446 2686 2851 3178 3658 4006 5077 6165 7737
Size: 240 165 327 480 348 1071 1088 1572
Cuts at: 7737 2446
Size: 3131
Fragments arranged by size:
3131 1572 1088 1071 480 348 327 240
165
ApaI G_GGCC'C
Cuts at: 2587 3565 3770 4371 2587
Size: 978 205 601 6638
Fragments arranged by size:
6638 978 601 205
ApaLI G'TGCA_C
Cuts at: 3080 6479 7635 3080
Size: 3399 1156 3867
Fragments arranged by size:
3867 3399 1156
ApoI r'AATT_y
Cuts at: 1493 3485 5731 5957 6109 8218 1493
Size: 1992 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1992 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6976 160
Size: 872 5944 1606
Fragments arranged by size:
5944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8422
AvaI C'yCGr_G
Cuts at: 1870 1943 2242 2543 2573 3123 3763 5747 1870
Size: 73 299 301 30 550 640 1984 4545
Fragments arranged by size:
4545 1984 640 550 301 299 73 30
BamHI G'GATC_C
Cuts at: 5752 6119 5752
Size: 367 8055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2759 3143 3720 3874
Size: 325 217 627 249 723 384 577 154
Cuts at: 3874 3914 4334 4358 4485 4547 5565 5743 7198
Size: 40 420 24 127 62 1018 178 1455
Cuts at: 7198 618
Size: 1842
Fragments arranged by size:
1842 1455 1018 723 627 577 420 384
325 249 217 178 154 127 62 40
24
BanII G_rGCy'C
Cuts at: 1391 2340 2587 2833 3369 3565 3678 3770 4371
Size: 949 247 246 536 196 113 92 601
Cuts at: 4371 4575 5251 5259 5315 5741 1391
Size: 204 676 8 56 426 4072
Fragments arranged by size:
4072 949 676 601 536 426 247 246
204 196 113 92 56 8
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 8422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3453 3594 928
Size: 971 1554 141 5756
Fragments arranged by size:
5756 1554 971 141
BbvCI CC'TCA_GC
Cuts at: 2172 3779 4107 2172
Size: 1607 328 6487
Fragments arranged by size:
6487 1607 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2233 2267 6613 6647 1903
Size: 34 296 34 4346 34 3678
Fragments arranged by size:
4346 3678 296 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6314 7524 6314
Size: 1210 7212
BclI T'GATC_A
Cuts at: 1548 4479 1548
Size: 2931 5491
BfrBI ATG'CAT
Cuts at: 4735 4735
Size: 8422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3688 3883 4028
Size: 107 122 71 631 605 2016 195 145
Cuts at: 4028 4579 7033 136
Size: 551 2454 1525
Fragments arranged by size:
2454 2016 1525 631 605 551 195 145
122 107 71
BglII A'GATC_T
Cuts at: 8417 8417
Size: 8422
BlpI GC'TnA_GC
Cuts at: 2915 4890 5839 2915
Size: 1975 949 5498
Fragments arranged by size:
5498 1975 949
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2587 2811 3084 3146 3196 3356 3565
Size: 571 226 224 273 62 50 160 209
Cuts at: 3565 3770 4371 4790 5162 6483 7639 1790
Size: 205 601 419 372 1321 1156 2573
Fragments arranged by size:
2573 1321 1156 601 571 419 372 273
226 224 209 205 160 62 50
BmgBI CAC'GTC
Cuts at: 1318 2371 2599 3202 4228 1318
Size: 1053 228 603 1026 5512
Fragments arranged by size:
5512 1053 1026 603 228
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3260 3334 5030
Size: 416 53 83 399 24 1843 74 1696
Cuts at: 5030 5521 5625 6153 7111 442
Size: 491 104 528 958 1753
Fragments arranged by size:
1843 1753 1696 958 528 491 416 399
104 83 74 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8422
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 8422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2068 2477 2765 3308 3353 3488 3944
Size: 802 27 409 288 543 45 135 456
Cuts at: 3944 4426 5273 7082 1239
Size: 482 847 1809 2579
Fragments arranged by size:
2579 1809 847 802 543 482 456 409
288 135 45 27
Bpu10I CC'TnA_GC
Cuts at: 2172 3779 4107 2172
Size: 1607 328 6487
Fragments arranged by size:
6487 1607 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5207 6547 7412 7710 5207
Size: 1340 865 298 5919
Fragments arranged by size:
5919 1340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2086 7085 882
Size: 617 587 4999 2219
Fragments arranged by size:
4999 2219 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 5216 6213 493
Size: 711 4012 997 2702
Fragments arranged by size:
4012 2702 997 711
BsaBI GATnn'nnATC
Cuts at: 8416 8416
Size: 8422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 4716 6229 6611
Size: 53 83 186 936 504 2679 1513 382
Cuts at: 6611 7246 275
Size: 635 1451
Fragments arranged by size:
2679 1513 1451 936 635 504 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3723 5562 6855 7253 7527 7674 8085 3723
Size: 1839 1293 398 274 147 411 4060
Fragments arranged by size:
4060 1839 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3602 3632 1178
Size: 30 165 30 2199 30 5968
Fragments arranged by size:
5968 2199 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 3377 3623 3626 3629 3767 4049 5447
Size: 1405 786 246 3 3 138 282 1398
Cuts at: 5447 1186
Size: 4161
Fragments arranged by size:
4161 1405 1398 786 282 246 138 3
3
BseYI C'CCAG_C
Cuts at: 2076 2108 2484 3022 3557 3693 3770 3861 3932
Size: 32 376 538 535 136 77 91 71
Cuts at: 3932 4188 4193 4874 4950 5227 5506 7625 8121
Size: 256 5 681 76 277 279 2119 496
Cuts at: 8121 2076
Size: 2377
Fragments arranged by size:
2377 2119 681 538 535 496 376 279
277 256 136 91 77 76 71 32
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2695 3824 4658 4726 5568 1163
Size: 573 501 458 1129 834 68 842 4017
Fragments arranged by size:
4017 1129 842 834 573 501 458 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5872 6633 7661 1540
Size: 275 186 2860 1011 761 1028 2301
Fragments arranged by size:
2860 2301 1028 1011 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 2833 3084 3369 3678 4378 5315 6483 6568
Size: 511 251 285 309 700 937 1168 85
Cuts at: 6568 7639 2322
Size: 1071 3105
Fragments arranged by size:
3105 1168 1071 937 700 511 309 285
251 85
BsmI GAATG_Cn'
Cuts at: 5933 6026 5933
Size: 93 8329
BsmBI CGTCTCn'nnnn_
Cuts at: 4707 4707
Size: 8422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2340 2361 2587 2811 2833 3084
Size: 399 532 18 21 226 224 22 251
Cuts at: 3084 3146 3196 3356 3369 3565 3678 3770 4371
Size: 62 50 160 13 196 113 92 601
Cuts at: 4371 4378 4575 4790 5162 5251 5259 5315 5741
Size: 7 197 215 372 89 8 56 426
Cuts at: 5741 6483 6568 7639 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 532 426 399 372
251 226 224 215 197 196 160 113
92 89 85 62 56 50 22 21
18 13 8 7
BspEI T'CCGG_A
Cuts at: 8085 8085
Size: 8422
BspHI T'CATG_A
Cuts at: 6311 8041 6311
Size: 1730 6692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 3315 3486 5777 844
Size: 1487 984 171 2291 3489
Fragments arranged by size:
3489 2291 1487 984 171
BsrBI CCG'CTC
Cuts at: 1394 1823 2581 3241 6309 1394
Size: 429 758 660 3068 3507
Fragments arranged by size:
3507 3068 758 660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4216 4600 5193 6917 7091 65
Size: 1183 2968 384 593 1724 174 1396
Fragments arranged by size:
2968 1724 1396 1183 593 384 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2056 2725 3278 4022 4544 4727
Size: 110 223 23 669 553 744 522 183
Cuts at: 4727 4917 5562 7066 7253 1700
Size: 190 645 1504 187 2869
Fragments arranged by size:
2869 1504 744 669 645 553 522 223
190 187 183 110 23
BsrGI T'GTAC_A
Cuts at: 96 3969 96
Size: 3873 4549
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8422
BssSI C'ACGA_G
Cuts at: 2255 6482 7494 2255
Size: 4227 1012 3183
Fragments arranged by size:
4227 3183 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 4009 5077 794
Size: 1652 1563 1068 4139
Fragments arranged by size:
4139 1652 1563 1068
BstBI TT'CG_AA
Cuts at: 7226 7226
Size: 8422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8422
BstXI CCAn_nnnn'nTGG
Cuts at: 4211 4819 4211
Size: 608 7814
BstYI r'GATC_y
Cuts at: 1972 3397 5074 5752 6119 6504 6521 7973 8059
Size: 1425 1677 678 367 385 17 1452 86
Cuts at: 8059 8071 8417 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1677 1452 1425 678 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5570 5570
Size: 8422
BtgI C'CryG_G
Cuts at: 513 1065 2298 2562 3576 4168 4204 4632 5586
Size: 552 1233 264 1014 592 36 428 954
Cuts at: 5586 513
Size: 3349
Fragments arranged by size:
3349 1233 1014 954 592 552 428 264
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7683
Fragments arranged by size:
7683 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3610 4143 4624 4763 5938 6732 6752 3064
Size: 546 533 481 139 1175 794 20 4734
Fragments arranged by size:
4734 1175 794 546 533 481 139 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4673 4708 529
Size: 35 4109 35 4243
Fragments arranged by size:
4243 4109 35 35
DraI TTT'AAA
Cuts at: 5726 5726
Size: 8422
DraIII CAC_nnn'GTG
Cuts at: 3268 3268
Size: 8422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2603 7429 809
Size: 507 1287 4826 1802
Fragments arranged by size:
4826 1802 1287 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2463 2565
Size: 54 1425 50 275 18 201 432 102
Cuts at: 2565 2643 2901 3276 3402 3897 4858 5346 5869
Size: 78 258 375 126 495 961 488 523
Cuts at: 5869 6758 8387 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 961 889 523 495 488 432
375 275 258 201 126 102 78 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 5869 1537
Size: 275 3046 1011 4090
Fragments arranged by size:
4090 3046 1011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 3010 3248 4772 5268 5361 6352 1353
Size: 1335 322 238 1524 496 93 991 3423
Fragments arranged by size:
3423 1524 1335 991 496 322 238 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 2534 4046 7009 7383 7529
Size: 1064 138 822 123 1512 2963 374 146
Cuts at: 7529 387
Size: 1280
Fragments arranged by size:
2963 1512 1280 1064 822 374 146 138
123
EcoICRI GAG'CTC
Cuts at: 2831 3367 3676 5313 2831
Size: 536 309 1637 5940
Fragments arranged by size:
5940 1637 536 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2068 2477 2765 3308 3353 3488
Size: 393 409 27 409 288 543 45 135
Cuts at: 3488 3680 3944 4426 5273 6485 7082 7869 8137
Size: 192 264 482 847 1212 597 787 268
Cuts at: 8137 1239
Size: 1524
Fragments arranged by size:
1524 1212 847 787 597 543 482 409
409 393 288 268 264 192 135 45
27
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 3341 4031 5573 2855
Size: 486 690 1542 5704
Fragments arranged by size:
5704 1542 690 486
EcoO109I rG'GnC_Cy
Cuts at: 2584 2749 3101 3175 3463 3475 3561 3562 3767
Size: 165 352 74 288 12 86 1 205
Cuts at: 3767 4368 4642 4975 2584
Size: 601 274 333 6031
Fragments arranged by size:
6031 601 352 333 288 274 205 165
86 74 12 1
EcoRI G'AATT_C
Cuts at: 5731 6109 5731
Size: 378 8044
FseI GG_CCGG'CC
Cuts at: 4026 4026
Size: 8422
FspI TGC'GCA
Cuts at: 5081 6928 5081
Size: 1847 6575
HaeII r_GCGC'y
Cuts at: 1678 2040 2077 4897 5389 5657 7569 1678
Size: 362 37 2820 492 268 1912 2531
Fragments arranged by size:
2820 2531 1912 492 362 268 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2820 2852 4664 4696 7063
Size: 32 135 32 1248 32 1812 32 2367
Cuts at: 7063 7095 7137 7169 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2367 1812 1248 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3064 3316 4148 5323 5766 6012
Size: 988 281 1126 252 832 1175 443 246
Cuts at: 6012 669
Size: 3079
Fragments arranged by size:
3079 1175 1126 988 832 443 281 252
246
HindIII A'AGCT_T
Cuts at: 748 5183 748
Size: 4435 3987
HpaI GTT'AAC
Cuts at: 6012 6012
Size: 8422
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 8422
KpnI G_GTAC'C
Cuts at: 3724 3918 5747 3724
Size: 194 1829 6399
Fragments arranged by size:
6399 1829 194
MfeI C'AATT_G
Cuts at: 6021 6021
Size: 8422
MluI A'CGCG_T
Cuts at: 8298 8298
Size: 8422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 4977 5631 7536 7720 1305
Size: 822 2850 654 1905 184 2007
Fragments arranged by size:
2850 2007 1905 822 654 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 2645 2903 3404 3899
Size: 54 1425 343 735 78 258 501 495
Cuts at: 3899 5348 10
Size: 1449 3084
Fragments arranged by size:
3084 1449 1425 735 501 495 343 258
78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2891 2897 4209 4269 4661 4817
Size: 648 1068 657 6 1312 60 392 156
Cuts at: 4817 5342 6380 6739 6898 518
Size: 525 1038 359 159 2042
Fragments arranged by size:
2042 1312 1068 1038 657 648 525 392
359 159 156 60 6
MspA1I CmG'CkG
Cuts at: 1606 2122 2209 2300 2488 2683 2719 3373 3421
Size: 516 87 91 188 195 36 654 48
Cuts at: 3421 3932 4006 4703 4940 4983 5838 6515 7663
Size: 511 74 697 237 43 855 677 1148
Cuts at: 7663 7908 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 855 697 677 654 516 511
245 237 195 188 91 87 74 48
43 36
NaeI GCC'GGC
Cuts at: 2035 2727 3280 4024 4546 4729 2035
Size: 692 553 744 522 183 5728
Fragments arranged by size:
5728 744 692 553 522 183
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 8422
NcoI C'CATG_G
Cuts at: 513 1065 3576 4204 4632 5586 513
Size: 552 2511 628 428 954 3349
Fragments arranged by size:
3349 2511 954 628 552 428
NdeI CA'TA_TG
Cuts at: 387 4064 387
Size: 3677 4745
NgoMIV G'CCGG_C
Cuts at: 2033 2725 3278 4022 4544 4727 2033
Size: 692 553 744 522 183 5728
Fragments arranged by size:
5728 744 692 553 522 183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8422
NotI GC'GGCC_GC
Cuts at: 5869 5869
Size: 8422
NsiI A_TGCA'T
Cuts at: 4737 4737
Size: 8422
NspI r_CATG'y
Cuts at: 1132 1229 2872 2896 3286 4735 4841 5494 5780
Size: 97 1643 24 390 1449 106 653 286
Cuts at: 5780 7325 1132
Size: 1545 2229
Fragments arranged by size:
2229 1643 1545 1449 653 390 286 106
97 24
PasI CC'CwG_GG
Cuts at: 1348 5644 1348
Size: 4296 4126
PciI A'CATG_T
Cuts at: 7321 7321
Size: 8422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 4441 5355 1108
Size: 1578 1755 914 4175
Fragments arranged by size:
4175 1755 1578 914
PfoI T'CCnGG_A
Cuts at: 2877 3171 3703 5268 2877
Size: 294 532 1565 6031
Fragments arranged by size:
6031 1565 532 294
PmeI GTTT'AAAC
Cuts at: 5726 5726
Size: 8422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6491 6523 8004 8036 6491
Size: 32 1481 32 6877
Fragments arranged by size:
6877 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 3175 3463 3475 2749
Size: 426 288 12 7696
Fragments arranged by size:
7696 426 288 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8422
PsiI TTA'TAA
Cuts at: 5992 5992
Size: 8422
PspOMI G'GGCC_C
Cuts at: 2583 3561 3766 4367 2583
Size: 978 205 601 6638
Fragments arranged by size:
6638 978 601 205
PstI C_TGCA'G
Cuts at: 830 2328 3605 3789 4138 4344 4770 5073 5774
Size: 1498 1277 184 349 206 426 303 701
Cuts at: 5774 830
Size: 3478
Fragments arranged by size:
3478 1498 1277 701 426 349 303 206
184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8422
PvuII CAG'CTG
Cuts at: 2209 2488 2719 3421 3932 4006 4703 4940 2209
Size: 279 231 702 511 74 697 237 5691
Fragments arranged by size:
5691 702 697 511 279 237 231 74
SacI G_AGCT'C
Cuts at: 2833 3369 3678 5315 2833
Size: 536 309 1637 5940
Fragments arranged by size:
5940 1637 536 309
SacII CC_GC'GG
Cuts at: 2301 2301
Size: 8422
SalI G'TCGA_C
Cuts at: 1936 5764 1936
Size: 3828 4594
SapI GCTCTTCn'nnn_
Cuts at: 2688 3248 4772 2688
Size: 560 1524 6338
Fragments arranged by size:
6338 1524 560
SbfI CC_TGCA'GG
Cuts at: 4344 5073 5774 4344
Size: 729 701 6992
Fragments arranged by size:
6992 729 701
ScaI AGT'ACT
Cuts at: 3188 6670 8382 3188
Size: 3482 1712 3228
Fragments arranged by size:
3482 3228 1712
SexAI A'CCwGG_T
Cuts at: 5421 5483 5421
Size: 62 8360
SfcI C'TryA_G
Cuts at: 826 1045 2324 3601 3785 3949 4134 4340 4766
Size: 219 1279 1277 184 164 185 206 426
Cuts at: 4766 5069 5770 6905 7586 7777 826
Size: 303 701 1135 681 191 1471
Fragments arranged by size:
1471 1279 1277 1135 701 681 426 303
219 206 191 185 184 164
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 8422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8422
SmaI CCC'GGG
Cuts at: 1872 3765 5749 1872
Size: 1893 1984 4545
Fragments arranged by size:
4545 1984 1893
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2242 3123 5186 6526 7427 7689
Size: 197 926 299 881 2063 1340 901 262
Cuts at: 7689 820
Size: 1553
Fragments arranged by size:
2063 1553 1340 926 901 881 299 262
197
SnaBI TAC'GTA
Cuts at: 493 6213 493
Size: 5720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8422
SphI G_CATG'C
Cuts at: 1132 2872 2896 3286 4735 4841 5780 1132
Size: 1740 24 390 1449 106 939 3774
Fragments arranged by size:
3774 1740 1449 939 390 106 24
SspI AAT'ATT
Cuts at: 5 52 6346 5
Size: 47 6294 2081
Fragments arranged by size:
6294 2081 47
StuI AGG'CCT
Cuts at: 1517 4509 1517
Size: 2992 5430
StyI C'CwwG_G
Cuts at: 513 1065 3576 4179 4204 4305 4632 4971 5586
Size: 552 2511 603 25 101 327 339 615
Cuts at: 5586 5694 5861 513
Size: 108 167 3074
Fragments arranged by size:
3074 2511 615 603 552 339 327 167
108 101 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2789 2841 3227 3279 3284 4202 4623
Size: 1255 1209 52 386 52 5 918 421
Cuts at: 4623 4655 4743 5290 6449 6466 6619 6804 7249
Size: 32 88 547 1159 17 153 185 445
Cuts at: 7249 325
Size: 1498
Fragments arranged by size:
1498 1255 1209 1159 918 547 445 421
386 185 153 88 52 52 32 17
5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3186 3969 6668
Size: 274 80 33 51 1443 1209 783 2699
Cuts at: 6668 8380 96
Size: 1712 138
Fragments arranged by size:
2699 1712 1443 1209 783 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 3455 3487 5038 5070 8382 8414 2801
Size: 32 622 32 1551 32 3312 32 2809
Fragments arranged by size:
3312 2809 1551 622 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8422
XbaI T'CTAG_A
Cuts at: 5758 5758
Size: 8422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 3701 5692 2638
Size: 1063 1991 5368
Fragments arranged by size:
5368 1991 1063
XhoI C'TCGA_G
Cuts at: 1943 2242 3123 1943
Size: 299 881 7242
Fragments arranged by size:
7242 881 299
XmaI C'CCGG_G
Cuts at: 1870 3763 5747 1870
Size: 1893 1984 4545
Fragments arranged by size:
4545 1984 1893
XmnI GAAnn'nnTTC
Cuts at: 6551 6551
Size: 8422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6230 276
Size: 53 83 186 936 4696 2468
Fragments arranged by size:
4696 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV FalI
FspAI NruI PacI PmlI PspXI PsrI RsrII SanDI
SfiI SrfI SwaI