Restriction map for
pFN21AE1086
(Circular) (Six-base) MAPSORT of: pFN21AE1086.seq Check: 9954 from: 1 to: 12637
pFN21AE1086 12637 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6548 6548
Size: 12637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10447 278
Size: 53 83 186 936 8911 2468
Fragments arranged by size:
8911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6846 7253 9013 9980 1937
Size: 4909 407 1760 967 4594
Fragments arranged by size:
4909 4594 1760 967 407
Acc65I G'GTAC_C
Cuts at: 4657 8828 9958 4657
Size: 4171 1130 7336
Fragments arranged by size:
7336 4171 1130
AclI AA'CG_TT
Cuts at: 1563 4944 10764 11137 1563
Size: 3381 5820 373 3063
Fragments arranged by size:
5820 3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4536 4619 5192 5831 5843 8906 10700
Size: 1775 1129 83 573 639 12 3063 1794
Cuts at: 10700 12084 12352 1632
Size: 1384 268 1917
Fragments arranged by size:
3063 1917 1794 1775 1384 1129 639 573
268 83 12
AflII C'TTAA_G
Cuts at: 820 1017 3014 3635 4546 6442 9049 820
Size: 197 1997 621 911 1896 2607 4408
Fragments arranged by size:
4408 2607 1997 1896 911 621 197
AflIII A'CryG_T
Cuts at: 1203 3361 4213 5500 7317 7569 7989 9478 9859
Size: 2158 852 1287 1817 252 420 1489 381
Cuts at: 9859 11536 12513 1203
Size: 1677 977 1327
Fragments arranged by size:
2158 1817 1677 1489 1327 1287 977 852
420 381 252
AgeI A'CCGG_T
Cuts at: 8689 11468 8689
Size: 2779 9858
AhdI GACnn_n'nnGTC
Cuts at: 11366 11366
Size: 12637
AleI CACnn'nnGTG
Cuts at: 1166 3629 7268 1166
Size: 2463 3639 6535
Fragments arranged by size:
6535 3639 2463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3058 1184
Size: 1874 10763
AlwNI CAG_nnn'CTG
Cuts at: 3673 4709 5036 5419 6799 10380 11952 3673
Size: 1036 327 383 1380 3581 1572 4358
Fragments arranged by size:
4358 3581 1572 1380 1036 383 327
ApaI G_GGCC'C
Cuts at: 3188 3982 5333 3188
Size: 794 1351 10492
Fragments arranged by size:
10492 1351 794
ApaLI G'TGCA_C
Cuts at: 3396 10694 11850 3396
Size: 7298 1156 4183
Fragments arranged by size:
7298 4183 1156
ApoI r'AATT_y
Cuts at: 1493 2109 2297 2605 2612 3068 3708 4051 4121
Size: 616 188 308 7 456 640 343 70
Cuts at: 4121 4435 6531 6788 7678 8448 8907 9023 9281
Size: 314 2096 257 890 770 459 116 258
Cuts at: 9281 9946 10172 10324 12433 1493
Size: 665 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 890 770 665 640 616
459 456 343 314 308 258 257 226
188 152 116 70 7
AseI AT'TA_AT
Cuts at: 160 1032 9039 11191 160
Size: 872 8007 2152 1606
Fragments arranged by size:
8007 2152 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12637
AvaI C'yCGr_G
Cuts at: 1870 1943 5332 9962 1870
Size: 73 3389 4630 4545
Fragments arranged by size:
4630 4545 3389 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6379 6412 9346 9379 6379
Size: 33 2934 33 9637
Fragments arranged by size:
9637 2934 33 33
BamHI G'GATC_C
Cuts at: 9967 10334 9967
Size: 367 12270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4657 8828 9958 11413 618
Size: 325 217 627 2870 4171 1130 1455 1842
Fragments arranged by size:
4171 2870 1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3188 3982 3992 4744 5333 5426 5633 6244
Size: 1797 794 10 752 589 93 207 611
Cuts at: 6244 9956 1391
Size: 3712 4072
Fragments arranged by size:
4072 3712 1797 794 752 611 589 207
93 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 3000 6882 928
Size: 971 573 528 3882 6683
Fragments arranged by size:
6683 3882 971 573 528
BbvCI CC'TCA_GC
Cuts at: 3263 7148 3263
Size: 3885 8752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 10828 10862 1903
Size: 34 371 34 8486 34 3678
Fragments arranged by size:
8486 3678 371 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2848 4597 6913 10529 11739 2127
Size: 721 1749 2316 3616 1210 3025
Fragments arranged by size:
3616 3025 2316 1749 1210 721
BclI T'GATC_A
Cuts at: 1548 5773 9637 1548
Size: 4225 3864 4548
Fragments arranged by size:
4548 4225 3864
BfrBI ATG'CAT
Cuts at: 7054 7054
Size: 12637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11248 136
Size: 107 122 71 631 605 9576 1525
Fragments arranged by size:
9576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8225 12632 8225
Size: 4407 8230
BlpI GC'TnA_GC
Cuts at: 10054 10054
Size: 12637
Bme1580I G_kGCm'C
Cuts at: 1790 3188 3400 3982 5161 5333 10698 11854 1790
Size: 1398 212 582 1179 172 5365 1156 2573
Fragments arranged by size:
5365 2573 1398 1179 1156 582 212 172
BmgBI CAC'GTC
Cuts at: 1318 4879 1318
Size: 3561 9076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3985 5204 6626
Size: 416 53 83 399 24 2568 1219 1422
Cuts at: 6626 7424 7660 10368 11326 442
Size: 798 236 2708 958 1753
Fragments arranged by size:
2708 2568 1753 1422 1219 958 798 416
399 236 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2805 8850 1056
Size: 1749 6045 4843
Fragments arranged by size:
6045 4843 1749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9302 9302
Size: 12637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 3701 4737 5792 6180 6441 7602 9765
Size: 803 1659 1036 1055 388 261 1161 2163
Cuts at: 9765 11297 1239
Size: 1532 2579
Fragments arranged by size:
2579 2163 1659 1532 1161 1055 1036 803
388 261
Bpu10I CC'TnA_GC
Cuts at: 3263 6345 7148 3263
Size: 3082 803 8752
Fragments arranged by size:
8752 3082 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3971 6033 6117 6228 7617 10762 11627 11925 3971
Size: 2062 84 111 1389 3145 865 298 4683
Fragments arranged by size:
4683 3145 2062 1389 865 298 111 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5819 6299 9157 9420 11300 882
Size: 617 4320 480 2858 263 1880 2219
Fragments arranged by size:
4320 2858 2219 1880 617 480 263
BsaAI yAC'GTr
Cuts at: 493 1204 7320 7873 10428 493
Size: 711 6116 553 2555 2702
Fragments arranged by size:
6116 2702 2555 711 553
BsaBI GATnn'nnATC
Cuts at: 4965 5322 12631 4965
Size: 357 7309 4971
Fragments arranged by size:
7309 4971 357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3298 10444 10826 11461
Size: 53 83 186 936 1765 7146 382 635
Cuts at: 11461 275
Size: 1451
Fragments arranged by size:
7146 1765 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2130 7713 8199 8689 11070 11468 11742 11889 12300
Size: 5583 486 490 2381 398 274 147 411
Cuts at: 12300 2130
Size: 2467
Fragments arranged by size:
5583 2467 2381 490 486 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4641 4671 9738 9768 1178
Size: 30 165 30 3238 30 5067 30 4047
Fragments arranged by size:
5067 4047 3238 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3245 6349 1186
Size: 2059 3104 7474
Fragments arranged by size:
7474 3104 2059
BseYI C'CCAG_C
Cuts at: 2056 3080 11840 12336 2056
Size: 1024 8760 496 2357
Fragments arranged by size:
8760 2357 1024 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 12064
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8338 10087 10848 11876 1540
Size: 275 186 6337 1749 761 1028 2301
Fragments arranged by size:
6337 2301 1749 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3006 3337 3400 3992 4744 5426 5840 6244 8726
Size: 331 63 592 752 682 414 404 2482
Cuts at: 8726 10698 10783 11854 3006
Size: 1972 85 1071 3789
Fragments arranged by size:
3789 2482 1972 1071 752 682 592 414
404 331 85 63
BsmI GAATG_Cn'
Cuts at: 3786 4876 7612 7775 9476 9718 10148 10241 3786
Size: 1090 2736 163 1701 242 430 93 6182
Fragments arranged by size:
6182 2736 1701 1090 430 242 163 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3308 3318 3308
Size: 10 12627
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3006 3188 3337 3400 3982 3992 4744
Size: 399 1216 182 149 63 582 10 752
Cuts at: 4744 5161 5333 5426 5633 5840 6244 8726 9956
Size: 417 172 93 207 207 404 2482 1230
Cuts at: 9956 10698 10783 11854 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2482 2174 1230 1216 1071 752 742 582
417 404 399 207 207 182 172 149
93 85 63 10
BspEI T'CCGG_A
Cuts at: 7713 12300 7713
Size: 4587 8050
BspHI T'CATG_A
Cuts at: 2229 2526 5752 8799 8973 9313 10526 12256 2229
Size: 297 3226 3047 174 340 1213 1730 2610
Fragments arranged by size:
3226 3047 2610 1730 1213 340 297 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6479 6548 9992 844
Size: 5635 69 3444 3489
Fragments arranged by size:
5635 3489 3444 69
BsrBI CCG'CTC
Cuts at: 1394 1823 8331 10524 1394
Size: 429 6508 2193 3507
Fragments arranged by size:
6508 3507 2193 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6708 8065 8535 11132 11306 65
Size: 1183 5460 1357 470 2597 174 1396
Fragments arranged by size:
5460 2597 1396 1357 1183 470 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8689 11281 11468 1700
Size: 110 6879 2592 187 2869
Fragments arranged by size:
6879 2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 2092 8341 96
Size: 1996 6249 4392
Fragments arranged by size:
6249 4392 1996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12637
BssSI C'ACGA_G
Cuts at: 6960 10697 11709 6960
Size: 3737 1012 7888
Fragments arranged by size:
7888 3737 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5027 5036 794
Size: 4233 9 8395
Fragments arranged by size:
8395 4233 9
BstBI TT'CG_AA
Cuts at: 5047 9091 9601 11441 5047
Size: 4044 510 1840 6243
Fragments arranged by size:
6243 4044 1840 510
BstEII G'GTnAC_C
Cuts at: 1184 6320 7808 1184
Size: 5136 1488 6013
Fragments arranged by size:
6013 5136 1488
BstXI CCAn_nnnn'nTGG
Cuts at: 5386 5386
Size: 12637
BstYI r'GATC_y
Cuts at: 1972 4447 6097 7119 8225 9967 10334 10719 10736
Size: 2475 1650 1022 1106 1742 367 385 17
Cuts at: 10736 12188 12274 12286 12632 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2475 1977 1742 1650 1452 1106 1022 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 7977 7977
Size: 12637
BtgI C'CryG_G
Cuts at: 513 1065 2004 3794 7213 7999 8157 513
Size: 552 939 1790 3419 786 158 4993
Fragments arranged by size:
4993 3419 1790 939 786 552 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11898
Fragments arranged by size:
11898 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 7158 9150 9384 10153 10947 10967 2068
Size: 5090 1992 234 769 794 20 3738
Fragments arranged by size:
5090 3738 1992 794 769 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8513 8548 529
Size: 35 7949 35 4618
Fragments arranged by size:
7949 4618 35 35
DraI TTT'AAA
Cuts at: 2407 2958 3828 4920 8734 8946 9941 2407
Size: 551 870 1092 3814 212 995 5103
Fragments arranged by size:
5103 3814 1092 995 870 551 212
DraIII CAC_nnn'GTG
Cuts at: 3911 6774 3911
Size: 2863 9774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8548 11644 809
Size: 507 7232 3096 1802
Fragments arranged by size:
7232 3096 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2516 7071 8326
Size: 54 1425 50 275 18 686 4555 1255
Cuts at: 8326 10084 10973 12602 8
Size: 1758 889 1629 43
Fragments arranged by size:
4555 1758 1629 1425 1255 889 686 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10084 1537
Size: 275 8272 4090
Fragments arranged by size:
8272 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2874 4161 4229 5862 8925 9070 9598 10567
Size: 1521 1287 68 1633 3063 145 528 969
Cuts at: 10567 1353
Size: 3423
Fragments arranged by size:
3423 3063 1633 1521 1287 969 528 145
68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11224 11598 11744 387
Size: 1064 138 9635 374 146 1280
Fragments arranged by size:
9635 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3990 4742 5424 6242 3990
Size: 752 682 818 10385
Fragments arranged by size:
10385 818 752 682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 3407 3701 4536 4619 4737 5192
Size: 393 410 1365 294 835 83 118 455
Cuts at: 5192 5792 5831 5843 6180 6441 7602 8906 9765
Size: 600 39 12 337 261 1161 1304 859
Cuts at: 9765 10700 11297 12084 12352 1239
Size: 935 597 787 268 1524
Fragments arranged by size:
1524 1365 1304 1161 935 859 835 787
600 597 455 410 393 337 294 268
261 118 83 39 12
EcoNI CCTnn'n_nnAGG
Cuts at: 9002 9002
Size: 12637
EcoO109I rG'GnC_Cy
Cuts at: 3979 5329 5492 9575 3979
Size: 1350 163 4083 7041
Fragments arranged by size:
7041 4083 1350 163
EcoRI G'AATT_C
Cuts at: 2297 4051 7678 9023 9946 10324 2297
Size: 1754 3627 1345 923 378 4610
Fragments arranged by size:
4610 3627 1754 1345 923 378
EcoRV GAT'ATC
Cuts at: 5341 7450 5341
Size: 2109 10528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4436 4624 5765 5950 4436
Size: 188 1141 185 11123
Fragments arranged by size:
11123 1141 188 185
FspI TGC'GCA
Cuts at: 11143 11143
Size: 12637
HaeII r_GCGC'y
Cuts at: 1678 9731 11784 1678
Size: 8053 2053 2531
Fragments arranged by size:
8053 2531 2053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2873 2905 3269 3301 4017
Size: 32 135 32 1301 32 364 32 716
Cuts at: 4017 4049 5330 5362 5827 5859 6029 6061 6568
Size: 32 1281 32 465 32 170 32 507
Cuts at: 6568 6600 6698 6730 7376 7408 9050 9082 9332
Size: 32 98 32 646 32 1642 32 250
Cuts at: 9332 9364 9622 9654 9738 9770 11278 11310 11352
Size: 32 258 32 84 32 1508 32 42
Cuts at: 11352 11384 1373
Size: 32 2626
Fragments arranged by size:
2626 1642 1508 1301 1281 716 646 507
465 364 258 250 170 135 98 84
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3566 3834 4142 6408 6728 8277
Size: 988 281 1628 268 308 2266 320 1549
Cuts at: 8277 9981 10227 669
Size: 1704 246 3079
Fragments arranged by size:
3079 2266 1704 1628 1549 988 320 308
281 268 246
HindIII A'AGCT_T
Cuts at: 748 2928 4281 5920 6175 7516 8666 748
Size: 2180 1353 1639 255 1341 1150 4719
Fragments arranged by size:
4719 2180 1639 1353 1341 1150 255
HpaI GTT'AAC
Cuts at: 8277 10227 8277
Size: 1950 10687
KpnI G_GTAC'C
Cuts at: 4661 8832 9962 4661
Size: 4171 1130 7336
Fragments arranged by size:
7336 4171 1130
MfeI C'AATT_G
Cuts at: 2658 2864 6326 8435 9490 10236 2658
Size: 206 3462 2109 1055 746 5059
Fragments arranged by size:
5059 3462 2109 1055 746 206
MluI A'CGCG_T
Cuts at: 12513 12513
Size: 12637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7563 7744 8620 9902 11751 11935 1305
Size: 6258 181 876 1282 1849 184 2007
Fragments arranged by size:
6258 2007 1849 1282 876 184 181
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2518 10
Size: 54 1425 343 686 10129
Fragments arranged by size:
10129 1425 686 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3793 3813 4212 5889 6554 7212
Size: 648 2463 164 20 399 1677 665 658
Cuts at: 7212 7268 8311 8573 8975 10595 10954 11113 518
Size: 56 1043 262 402 1620 359 159 2042
Fragments arranged by size:
2463 2042 1677 1620 1043 665 658 648
402 399 359 262 164 159 56 20
MspA1I CmG'CkG
Cuts at: 1606 3080 6254 7249 7511 8546 10053 10730 11878
Size: 1474 3174 995 262 1035 1507 677 1148
Cuts at: 11878 12123 1606
Size: 245 2120
Fragments arranged by size:
3174 2120 1507 1474 1148 1035 995 677
262 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3794 7213 7999 8157 513
Size: 552 939 1790 3419 786 158 4993
Fragments arranged by size:
4993 3419 1790 939 786 552 158
NdeI CA'TA_TG
Cuts at: 387 8308 8613 9097 387
Size: 7921 305 484 3927
Fragments arranged by size:
7921 3927 484 305
NheI G'CTAG_C
Cuts at: 1052 2801 8846 1052
Size: 1749 6045 4843
Fragments arranged by size:
6045 4843 1749
NotI GC'GGCC_GC
Cuts at: 10084 10084
Size: 12637
NsiI A_TGCA'T
Cuts at: 7056 7056
Size: 12637
NspI r_CATG'y
Cuts at: 1132 1229 3365 4168 4217 4574 5413 5504 6353
Size: 97 2136 803 49 357 839 91 849
Cuts at: 6353 7286 7573 7993 9482 9612 9863 9995 11540
Size: 933 287 420 1489 130 251 132 1545
Cuts at: 11540 1132
Size: 2229
Fragments arranged by size:
2229 2136 1545 1489 933 849 839 803
420 357 287 251 132 130 97 91
49
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12637
PciI A'CATG_T
Cuts at: 3361 4213 5500 7569 7989 9478 9859 11536 3361
Size: 852 1287 2069 420 1489 381 1677 4462
Fragments arranged by size:
4462 2069 1677 1489 1287 852 420 381
PflMI CCAn_nnn'nTGG
Cuts at: 1108 8864 1108
Size: 7756 4881
PfoI T'CCnGG_A
Cuts at: 3762 5808 8863 3762
Size: 2046 3055 7536
Fragments arranged by size:
7536 3055 2046
PmeI GTTT'AAAC
Cuts at: 9941 9941
Size: 12637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10706 10738 12219 12251 10706
Size: 32 1481 32 11092
Fragments arranged by size:
11092 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5492 9575 5492
Size: 4083 8554
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12637
PsiI TTA'TAA
Cuts at: 4529 10207 4529
Size: 5678 6959
PspOMI G'GGCC_C
Cuts at: 3184 3978 5329 3184
Size: 794 1351 10492
Fragments arranged by size:
10492 1351 794
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4401 4433 7482 7514 4401
Size: 32 3049 32 9524
Fragments arranged by size:
9524 3049 32 32
PstI C_TGCA'G
Cuts at: 830 3057 3987 5032 5698 7782 9391 9989 830
Size: 2227 930 1045 666 2084 1609 598 3478
Fragments arranged by size:
3478 2227 2084 1609 1045 930 666 598
PvuI CG_AT'CG
Cuts at: 2001 8338 2001
Size: 6337 6300
PvuII CAG'CTG
Cuts at: 3080 6254 7249 7511 8546 3080
Size: 3174 995 262 1035 7171
Fragments arranged by size:
7171 3174 1035 995 262
SacI G_AGCT'C
Cuts at: 3992 4744 5426 6244 3992
Size: 752 682 818 10385
Fragments arranged by size:
10385 818 752 682
SalI G'TCGA_C
Cuts at: 1936 9979 1936
Size: 8043 4594
SanDI GG'GwC_CC
Cuts at: 5492 5492
Size: 12637
SapI GCTCTTCn'nnn_
Cuts at: 2874 9598 2874
Size: 6724 5913
SbfI CC_TGCA'GG
Cuts at: 9989 9989
Size: 12637
ScaI AGT'ACT
Cuts at: 3176 8138 10885 12597 3176
Size: 4962 2747 1712 3216
Fragments arranged by size:
4962 3216 2747 1712
SfcI C'TryA_G
Cuts at: 826 1045 2160 3053 3591 3643 3868 3983 4228
Size: 219 1115 893 538 52 225 115 245
Cuts at: 4228 5028 5694 6292 6544 7684 7778 9387 9985
Size: 800 666 598 252 1140 94 1609 598
Cuts at: 9985 11120 11801 11992 826
Size: 1135 681 191 1471
Fragments arranged by size:
1609 1471 1140 1135 1115 893 800 681
666 598 598 538 252 245 225 219
191 115 94 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12637
SmaI CCC'GGG
Cuts at: 1872 9964 1872
Size: 8092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3014 3635 3950 4546 6048 6132
Size: 197 926 1071 621 315 596 1502 84
Cuts at: 6132 6243 6442 7632 9049 10741 11642 11904 820
Size: 111 199 1190 1417 1692 901 262 1553
Fragments arranged by size:
1692 1553 1502 1417 1190 1071 926 901
621 596 315 262 199 197 111 84
SnaBI TAC'GTA
Cuts at: 493 10428 493
Size: 9935 2702
SpeI A'CTAG_T
Cuts at: 152 7477 8017 152
Size: 7325 540 4772
Fragments arranged by size:
7325 4772 540
SphI G_CATG'C
Cuts at: 1132 7286 9995 1132
Size: 6154 2709 3774
Fragments arranged by size:
6154 3774 2709
SspI AAT'ATT
Cuts at: 5 52 2941 3127 3945 7009 9445 10561 5
Size: 47 2889 186 818 3064 2436 1116 2081
Fragments arranged by size:
3064 2889 2436 2081 1116 818 186 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 12637
StyI C'CwwG_G
Cuts at: 513 1065 2004 3794 4506 5221 7138 7213 7692
Size: 552 939 1790 712 715 1917 75 479
Cuts at: 7692 7899 7999 8157 10076 513
Size: 207 100 158 1919 3074
Fragments arranged by size:
3074 1919 1917 1790 939 715 712 552
479 207 158 100 75
SwaI ATTT'AAAT
Cuts at: 2958 2958
Size: 12637
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3304 9130 10664 10681 10834 11019 11464
Size: 1255 1724 5826 1534 17 153 185 445
Cuts at: 11464 325
Size: 1498
Fragments arranged by size:
5826 1724 1534 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 3174 3512
Size: 274 80 33 51 1443 115 1082 338
Cuts at: 3512 4151 6734 8136 8341 10883 12595 96
Size: 639 2583 1402 205 2542 1712 138
Fragments arranged by size:
2583 2542 1712 1443 1402 1082 639 338
274 205 138 115 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 12597 12629 12597
Size: 32 12605
Tth111I GACn'n_nGTC
Cuts at: 1316 4109 1316
Size: 2793 9844
XbaI T'CTAG_A
Cuts at: 6901 8671 9973 6901
Size: 1770 1302 9565
Fragments arranged by size:
9565 1770 1302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3697 9914 3697
Size: 6217 6420
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12637
XmaI C'CCGG_G
Cuts at: 1870 9962 1870
Size: 8092 4545
XmnI GAAnn'nnTTC
Cuts at: 2769 3420 3663 4824 8704 8859 9444 10766 2769
Size: 651 243 1161 3880 155 585 1322 4640
Fragments arranged by size:
4640 3880 1322 1161 651 585 243 155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10445 276
Size: 53 83 186 936 8911 2468
Fragments arranged by size:
8911 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI RsrII SacII SexAI SfiI SfoI SrfI