Restriction map for
pFN21AE1085
(Circular) (Six-base) MAPSORT of: pFN21AE1085.seq Check: 3035 from: 1 to: 7258
pFN21AE1085 7258 bp
With 182 enzymes: SgfI *
July 28, 2009 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2608 2074
Size: 534 6724
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2252 5068 278
Size: 53 83 186 936 716 2816 2468
Fragments arranged by size:
2816 2468 936 716 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4601 1937
Size: 2664 4594
Acc65I G'GTAC_C
Cuts at: 4579 4579
Size: 7258
AclI AA'CG_TT
Cuts at: 1563 5385 5758 1563
Size: 3822 373 3063
Fragments arranged by size:
3822 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4157 4163 5321 6705 6973 1632
Size: 2525 6 1158 1384 268 1917
Fragments arranged by size:
2525 1917 1384 1158 268 6
AflII C'TTAA_G
Cuts at: 820 1017 2693 820
Size: 197 1676 5385
Fragments arranged by size:
5385 1676 197
AflIII A'CryG_T
Cuts at: 1203 2185 6157 7134 1203
Size: 982 3972 977 1327
Fragments arranged by size:
3972 1327 982 977
AgeI A'CCGG_T
Cuts at: 2533 6089 2533
Size: 3556 3702
AhdI GACnn_n'nnGTC
Cuts at: 5987 5987
Size: 7258
AleI CACnn'nnGTG
Cuts at: 1166 2534 3869 1166
Size: 1368 1335 4555
Fragments arranged by size:
4555 1368 1335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2043 1184
Size: 859 6399
AlwNI CAG_nnn'CTG
Cuts at: 2017 2911 3044 3073 4060 5001 6573 2017
Size: 894 133 29 987 941 1572 2702
Fragments arranged by size:
2702 1572 987 941 894 133 29
ApaI G_GGCC'C
Cuts at: 3067 3103 3429 3067
Size: 36 326 6896
Fragments arranged by size:
6896 326 36
ApaLI G'TGCA_C
Cuts at: 5315 6471 5315
Size: 1156 6102
ApoI r'AATT_y
Cuts at: 1493 4567 4793 4945 7054 1493
Size: 3074 226 152 2109 1697
Fragments arranged by size:
3074 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5812 160
Size: 872 4780 1606
Fragments arranged by size:
4780 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7258
AvaI C'yCGr_G
Cuts at: 1870 1943 2408 3292 3699 3917 4583 1870
Size: 73 465 884 407 218 666 4545
Fragments arranged by size:
4545 884 666 465 407 218 73
BamHI G'GATC_C
Cuts at: 4588 4955 4588
Size: 367 6891
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2536 2553 2585 2807 3047
Size: 325 217 627 749 17 32 222 240
Cuts at: 3047 3416 3933 4579 6034 618
Size: 369 517 646 1455 1842
Fragments arranged by size:
1842 1455 749 646 627 517 369 325
240 222 217 32 17
BanII G_rGCy'C
Cuts at: 1391 2219 2444 2748 3067 3103 3131 3360 3375
Size: 828 225 304 319 36 28 229 15
Cuts at: 3375 3429 4535 4577 1391
Size: 54 1106 42 4072
Fragments arranged by size:
4072 1106 828 319 304 229 225 54
42 36 28 15
BbeI G_GCGC'C
Cuts at: 3051 3051
Size: 7258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3273 4151 928
Size: 971 1374 878 4035
Fragments arranged by size:
4035 1374 971 878
BbvCI CC'TCA_GC
Cuts at: 3095 3726 3095
Size: 631 6627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5449 5483 1903
Size: 34 3512 34 3678
Fragments arranged by size:
3678 3512 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2980 5150 6360 2980
Size: 2170 1210 3878
Fragments arranged by size:
3878 2170 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2933 3118 3379
Size: 107 122 71 631 605 1261 185 261
Cuts at: 3379 3800 4214 4280 4289 5869 136
Size: 421 414 66 9 1580 1525
Fragments arranged by size:
1580 1525 1261 631 605 421 414 261
185 122 107 71 66 9
BglII A'GATC_T
Cuts at: 7253 7253
Size: 7258
BlpI GC'TnA_GC
Cuts at: 3773 4675 3773
Size: 902 6356
Bme1580I G_kGCm'C
Cuts at: 1790 2556 2590 2810 3067 3103 3173 3180 3348
Size: 766 34 220 257 36 70 7 168
Cuts at: 3348 3419 3429 4389 5319 6475 1790
Size: 71 10 960 930 1156 2573
Fragments arranged by size:
2573 1156 960 930 766 257 220 168
71 70 36 34 10 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2448 2538 2774
Size: 416 53 83 399 24 1031 90 236
Cuts at: 2774 3167 4989 5947 442
Size: 393 1822 958 1753
Fragments arranged by size:
1822 1753 1031 958 416 399 393 236
90 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3583 4446 3325
Size: 258 863 6137
Fragments arranged by size:
6137 863 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2624 2630 2645 2810 2900 3095 3485
Size: 1127 258 6 15 165 90 195 390
Cuts at: 3485 3532 5918 1239
Size: 47 2386 2579
Fragments arranged by size:
2579 2386 1127 390 258 195 165 90
47 15 6
Bpu10I CC'TnA_GC
Cuts at: 2213 2922 3095 3644 3726 2213
Size: 709 173 549 82 5745
Fragments arranged by size:
5745 709 549 173 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2876 5383 6248 6546 2129
Size: 747 2507 865 298 2841
Fragments arranged by size:
2841 2507 865 747 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2342 2786 2876 5921 882
Size: 617 603 240 444 90 3045 2219
Fragments arranged by size:
3045 2219 617 603 444 240 90
BsaAI yAC'GTr
Cuts at: 493 1204 2023 2152 2566 3979 5049 493
Size: 711 819 129 414 1413 1070 2702
Fragments arranged by size:
2702 1413 1070 819 711 414 129
BsaBI GATnn'nnATC
Cuts at: 3221 7252 3221
Size: 4031 3227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2249 3048 5065 5447
Size: 53 83 186 936 716 799 2017 382
Cuts at: 5447 6082 275
Size: 635 1451
Fragments arranged by size:
2017 1451 936 799 716 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2533 3489 5691 6089 6363 6510 6921 2533
Size: 956 2202 398 274 147 411 2870
Fragments arranged by size:
2870 2202 956 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3152 3182 1178
Size: 30 165 30 1749 30 5254
Fragments arranged by size:
5254 1749 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 3419 3479 3508 3767 3770 1186
Size: 1441 792 60 29 259 3 4674
Fragments arranged by size:
4674 1441 792 259 60 29 3
BseYI C'CCAG_C
Cuts at: 2053 2886 2943 3639 4388 6461 6957 2053
Size: 833 57 696 749 2073 496 2354
Fragments arranged by size:
2354 2073 833 749 696 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2003 2216 4001 4073 1163
Size: 573 267 213 1785 72 4348
Fragments arranged by size:
4348 1785 573 267 213 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 2463 4708 5469 6497 1540
Size: 275 186 316 146 2245 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 316 275 186 146
BsiHKAI G_wGCw'C
Cuts at: 2219 2270 2344 2640 2661 2748 3335 4350 4427
Size: 51 74 296 21 87 587 1015 77
Cuts at: 4427 4505 5319 5404 6475 2219
Size: 78 814 85 1071 3002
Fragments arranged by size:
3002 1071 1015 814 587 296 87 85
78 77 74 51 21
BsmI GAATG_Cn'
Cuts at: 4769 4862 4769
Size: 93 7165
BsmBI CGTCTCn'nnnn_
Cuts at: 2411 2411
Size: 7258
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2270 2344 2444 2556 2590 2640
Size: 399 429 51 74 100 112 34 50
Cuts at: 2640 2661 2748 2810 3067 3103 3131 3173 3180
Size: 21 87 62 257 36 28 42 7
Cuts at: 3180 3335 3348 3360 3375 3419 3429 4350 4389
Size: 155 13 12 15 44 10 921 39
Cuts at: 4389 4427 4505 4535 4577 5319 5404 6475 1391
Size: 38 78 30 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 921 742 429 399 257 155
112 100 87 85 78 74 62 51
50 44 42 42 39 38 36 34
30 28 21 15 13 12 10 7
BspEI T'CCGG_A
Cuts at: 6921 6921
Size: 7258
BspHI T'CATG_A
Cuts at: 3450 5147 6877 3450
Size: 1697 1730 3831
Fragments arranged by size:
3831 1730 1697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2608 4613 844
Size: 1230 534 2005 3489
Fragments arranged by size:
3489 2005 1230 534
BsrBI CCG'CTC
Cuts at: 1394 1823 3844 5145 1394
Size: 429 2021 1301 3507
Fragments arranged by size:
3507 2021 1301 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3114 5753 5927 65
Size: 1183 1866 2639 174 1396
Fragments arranged by size:
2639 1866 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2432 2462 2533 3419 3678 5902 6089
Size: 110 622 30 71 886 259 2224 187
Cuts at: 6089 1700
Size: 2869
Fragments arranged by size:
2869 2224 886 622 259 187 110 71
30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7258
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7258
BssSI C'ACGA_G
Cuts at: 5318 6330 5318
Size: 1012 6246
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 4060 794
Size: 2117 1149 3992
Fragments arranged by size:
3992 2117 1149
BstBI TT'CG_AA
Cuts at: 6062 6062
Size: 7258
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7258
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2437
Size: 7258
BstYI r'GATC_y
Cuts at: 1972 4588 4955 5340 5357 6809 6895 6907 7253
Size: 2616 367 385 17 1452 86 12 346
Cuts at: 7253 1972
Size: 1977
Fragments arranged by size:
2616 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2227 2317 2353 2369 2510
Size: 552 939 99 124 90 36 16 141
Cuts at: 2510 3011 3480 4047 4249 4323 513
Size: 501 469 567 202 74 3448
Fragments arranged by size:
3448 939 567 552 501 469 202 141
124 99 90 74 36 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3255 3973 4431 487
Size: 683 56 2029 718 458 3314
Fragments arranged by size:
3314 2029 718 683 458 56
BtsI GCAGTG_nn'
Cuts at: 2199 3720 4774 5568 5588 2199
Size: 1521 1054 794 20 3869
Fragments arranged by size:
3869 1521 1054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2260 2295 529
Size: 35 1696 35 5492
Fragments arranged by size:
5492 1696 35 35
DraI TTT'AAA
Cuts at: 4562 4562
Size: 7258
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2975 3980 4073 6265 809
Size: 507 1659 1005 93 2192 1802
Fragments arranged by size:
2192 1802 1659 1005 507 93
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2421 2460
Size: 54 1425 50 275 18 484 107 39
Cuts at: 2460 2957 3030 3627 3666 4134 4239 4351 4380
Size: 497 73 597 39 468 105 112 29
Cuts at: 4380 4705 5594 7223 8
Size: 325 889 1629 43
Fragments arranged by size:
1629 1425 889 597 497 484 468 325
275 112 107 105 73 54 50 43
39 39 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 2460 4705 1537
Size: 275 502 146 2245 4090
Fragments arranged by size:
4090 2245 502 275 146
EarI CTCTTCn'nnn_
Cuts at: 1353 3323 4184 4548 5188 1353
Size: 1970 861 364 640 3423
Fragments arranged by size:
3423 1970 861 640 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5845 6219 6365 387
Size: 1064 138 4256 374 146 1280
Fragments arranged by size:
4256 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2217 2746 2217
Size: 529 6729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2624 2630 2645 2810 2900 3095
Size: 393 734 258 6 15 165 90 195
Cuts at: 3095 3485 3532 4157 4163 5321 5918 6705 6973
Size: 390 47 625 6 1158 597 787 268
Cuts at: 6973 1239
Size: 1524
Fragments arranged by size:
1524 1158 787 734 625 597 393 390
268 258 195 165 90 47 15 6
6
EcoO109I rG'GnC_Cy
Cuts at: 2014 2043 2083 2776 3099 3100 3426 3595 2014
Size: 29 40 693 323 1 326 169 5677
Fragments arranged by size:
5677 693 326 323 169 40 29 1
EcoRI G'AATT_C
Cuts at: 4567 4945 4567
Size: 378 6880
FspI TGC'GCA
Cuts at: 5764 5764
Size: 7258
HaeII r_GCGC'y
Cuts at: 1678 2203 2903 3051 6405 1678
Size: 525 700 148 3354 2531
Fragments arranged by size:
3354 2531 700 525 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3521 3553 3719 3751 3773
Size: 32 135 32 1949 32 166 32 22
Cuts at: 3773 3805 4304 4336 4454 4486 5899 5931 5973
Size: 32 499 32 118 32 1413 32 42
Cuts at: 5973 6005 1373
Size: 32 2626
Fragments arranged by size:
2626 1949 1413 499 166 135 118 42
32 32 32 32 32 32 32 32
32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3517 3985 4300 4602 4848 669
Size: 988 281 1579 468 315 302 246 3079
Fragments arranged by size:
3079 1579 988 468 315 302 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7258
HpaI GTT'AAC
Cuts at: 4848 4848
Size: 7258
KasI G'GCGC_C
Cuts at: 3047 3047
Size: 7258
KpnI G_GTAC'C
Cuts at: 4583 4583
Size: 7258
MfeI C'AATT_G
Cuts at: 4857 4857
Size: 7258
MluI A'CGCG_T
Cuts at: 2185 7134 2185
Size: 4949 2309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6372 6556 1305
Size: 5067 184 2007
Fragments arranged by size:
5067 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2959 3032 4136 10
Size: 54 1425 343 1127 73 1104 3132
Fragments arranged by size:
3132 1425 1127 1104 343 73 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 3869 5216 5575 5734 518
Size: 648 1368 1335 1347 359 159 2042
Fragments arranged by size:
2042 1368 1347 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2355 2371 2608 2726 3044 3061 3182 3665
Size: 749 16 237 118 318 17 121 483
Cuts at: 3665 3694 4060 4071 4392 4674 5351 6499 6744
Size: 29 366 11 321 282 677 1148 245
Cuts at: 6744 1606
Size: 2120
Fragments arranged by size:
2120 1148 749 677 483 366 321 318
282 245 237 121 118 29 17 16
11
NaeI GCC'GGC
Cuts at: 2464 3680 2464
Size: 1216 6042
NarI GG'CG_CC
Cuts at: 3048 3048
Size: 7258
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5767
Fragments arranged by size:
5767 939 552
NdeI CA'TA_TG
Cuts at: 387 3204 387
Size: 2817 4441
NgoMIV G'CCGG_C
Cuts at: 2462 3678 2462
Size: 1216 6042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7258
NotI GC'GGCC_GC
Cuts at: 4705 4705
Size: 7258
NruI TCG'CGA
Cuts at: 4416 4416
Size: 7258
NspI r_CATG'y
Cuts at: 1132 1229 2839 4616 6161 1132
Size: 97 1610 1777 1545 2229
Fragments arranged by size:
2229 1777 1610 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2039 1348
Size: 691 6567
PciI A'CATG_T
Cuts at: 6157 6157
Size: 7258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3073 3154 3508 1108
Size: 1965 81 354 4858
Fragments arranged by size:
4858 1965 354 81
PfoI T'CCnGG_A
Cuts at: 2661 2661
Size: 7258
PmeI GTTT'AAAC
Cuts at: 4562 4562
Size: 7258
PmlI CAC'GTG
Cuts at: 2023 2566 2023
Size: 543 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5327 5359 6840 6872 5327
Size: 32 1481 32 5713
Fragments arranged by size:
5713 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2043 2083 2776 2014
Size: 29 40 693 6496
Fragments arranged by size:
6496 693 40 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7258
PsiI TTA'TAA
Cuts at: 4828 4828
Size: 7258
PspOMI G'GGCC_C
Cuts at: 3063 3099 3425 3063
Size: 36 326 6896
Fragments arranged by size:
6896 326 36
PstI C_TGCA'G
Cuts at: 830 2907 2916 3187 3655 3879 4610 830
Size: 2077 9 271 468 224 731 3478
Fragments arranged by size:
3478 2077 731 468 271 224 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7258
PvuII CAG'CTG
Cuts at: 2608 3694 4060 4392 2608
Size: 1086 366 332 5474
Fragments arranged by size:
5474 1086 366 332
RsrII CG'GwC_CG
Cuts at: 2977 2977
Size: 7258
SacI G_AGCT'C
Cuts at: 2219 2748 2219
Size: 529 6729
SacII CC_GC'GG
Cuts at: 2356 2372 2356
Size: 16 7242
SalI G'TCGA_C
Cuts at: 1936 4600 1936
Size: 2664 4594
SanDI GG'GwC_CC
Cuts at: 2043 2043
Size: 7258
SapI GCTCTTCn'nnn_
Cuts at: 3323 4184 3323
Size: 861 6397
SbfI CC_TGCA'GG
Cuts at: 3655 4610 3655
Size: 955 6303
ScaI AGT'ACT
Cuts at: 5506 7218 5506
Size: 1712 5546
SexAI A'CCwGG_T
Cuts at: 3936 3936
Size: 7258
SfcI C'TryA_G
Cuts at: 826 1045 2903 2912 3183 3651 3875 4108 4606
Size: 219 1858 9 271 468 224 233 498
Cuts at: 4606 5741 6422 6613 826
Size: 1135 681 191 1471
Fragments arranged by size:
1858 1471 1135 681 498 468 271 233
224 219 191 9
SfoI GGC'GCC
Cuts at: 3049 3049
Size: 7258
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7258
SgrAI Cr'CCGG_yG
Cuts at: 1700 2533 3419 1700
Size: 833 886 5539
Fragments arranged by size:
5539 886 833
SmaI CCC'GGG
Cuts at: 1872 3294 4585 1872
Size: 1422 1291 4545
Fragments arranged by size:
4545 1422 1291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2408 2693 2891 3699 5362
Size: 197 926 201 264 285 198 808 1663
Cuts at: 5362 6263 6525 820
Size: 901 262 1553
Fragments arranged by size:
1663 1553 926 901 808 285 264 262
201 198 197
SnaBI TAC'GTA
Cuts at: 493 5049 493
Size: 4556 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7258
SphI G_CATG'C
Cuts at: 1132 2839 4616 1132
Size: 1707 1777 3774
Fragments arranged by size:
3774 1777 1707
SspI AAT'ATT
Cuts at: 5 52 5182 5
Size: 47 5130 2081
Fragments arranged by size:
5130 2081 47
StuI AGG'CCT
Cuts at: 1517 2954 3121 3255 3649 1517
Size: 1437 167 134 394 5126
Fragments arranged by size:
5126 1437 394 167 134
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2865 4443 4697 513
Size: 552 939 75 786 1578 254 3074
Fragments arranged by size:
3074 1578 939 786 552 254 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 3365 3377 5285 5302 5455 5640
Size: 1255 863 922 12 1908 17 153 185
Cuts at: 5640 6085 325
Size: 445 1498
Fragments arranged by size:
1908 1498 1255 922 863 445 185 153
17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2686 5504 7216
Size: 274 80 33 51 1443 709 2818 1712
Cuts at: 7216 96
Size: 138
Fragments arranged by size:
2818 1712 1443 709 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 3123 3152 3155 3184 7218 7250 2055
Size: 32 1036 29 3 29 4034 32 2063
Fragments arranged by size:
4034 2063 1036 32 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 4265 1316
Size: 2949 4309
XbaI T'CTAG_A
Cuts at: 4594 4594
Size: 7258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4045 4045
Size: 7258
XhoI C'TCGA_G
Cuts at: 1943 2408 3699 1943
Size: 465 1291 5502
Fragments arranged by size:
5502 1291 465
XmaI C'CCGG_G
Cuts at: 1870 3292 4583 1870
Size: 1422 1291 4545
Fragments arranged by size:
4545 1422 1291
XmnI GAAnn'nnTTC
Cuts at: 5387 5387
Size: 7258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2250 5066 276
Size: 53 83 186 936 716 2816 2468
Fragments arranged by size:
2816 2468 936 716 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI
PacI PspXI PsrI SfiI SrfI SwaI