Restriction map for
pFN21AE1081
(Circular) (Six-base) MAPSORT of: pFN21AE1081.seq Check: 8519 from: 1 to: 9184
pFN21AE1081 9184 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3318 3318
Size: 9184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2307 3914 6994 278
Size: 53 83 186 936 771 1607 3080 2468
Fragments arranged by size:
3080 2468 1607 936 771 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 5371 6527 1937
Size: 1872 1562 1156 4594
Fragments arranged by size:
4594 1872 1562 1156
Acc65I G'GTAC_C
Cuts at: 6505 6505
Size: 9184
AclI AA'CG_TT
Cuts at: 1563 4776 7311 7684 1563
Size: 3213 2535 373 3063
Fragments arranged by size:
3213 3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 2694 5952 7247 8631 8899 1632
Size: 861 201 3258 1295 1384 268 1917
Fragments arranged by size:
3258 1917 1384 1295 861 268 201
AflII C'TTAA_G
Cuts at: 820 1017 3376 820
Size: 197 2359 6628
Fragments arranged by size:
6628 2359 197
AflIII A'CryG_T
Cuts at: 1203 8083 9060 1203
Size: 6880 977 1327
Fragments arranged by size:
6880 1327 977
AgeI A'CCGG_T
Cuts at: 8015 8015
Size: 9184
AhdI GACnn_n'nnGTC
Cuts at: 5637 7913 5637
Size: 2276 6908
AleI CACnn'nnGTG
Cuts at: 1166 3803 1166
Size: 2637 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9184
AlwNI CAG_nnn'CTG
Cuts at: 2566 2990 6927 8499 2566
Size: 424 3937 1572 3251
Fragments arranged by size:
3937 3251 1572 424
ApaLI G'TGCA_C
Cuts at: 5378 7241 8397 5378
Size: 1863 1156 6165
Fragments arranged by size:
6165 1863 1156
ApoI r'AATT_y
Cuts at: 1493 2081 2700 2836 3115 3170 3565 3663 3730
Size: 588 619 136 279 55 395 98 67
Cuts at: 3730 4787 5061 5231 5319 5438 5897 6493 6719
Size: 1057 274 170 88 119 459 596 226
Cuts at: 6719 6871 8980 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1057 619 596 588 459 395
279 274 226 170 152 136 119 98
88 67 55
AseI AT'TA_AT
Cuts at: 160 1032 7738 160
Size: 872 6706 1606
Fragments arranged by size:
6706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9184
AvaI C'yCGr_G
Cuts at: 1870 1943 2598 6296 6405 6509 1870
Size: 73 655 3698 109 104 4545
Fragments arranged by size:
4545 3698 655 109 104 73
BamHI G'GATC_C
Cuts at: 4709 6514 6881 4709
Size: 1805 367 7012
Fragments arranged by size:
7012 1805 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6053 6505 7960 618
Size: 325 217 627 4266 452 1455 1842
Fragments arranged by size:
4266 1842 1455 627 452 325 217
BanII G_rGCy'C
Cuts at: 1391 2848 2867 5160 6503 1391
Size: 1457 19 2293 1343 4072
Fragments arranged by size:
4072 2293 1457 1343 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2883 3891 5101 5534 928
Size: 971 984 1008 1210 433 4578
Fragments arranged by size:
4578 1210 1008 984 971 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7375 7409 1903
Size: 34 5438 34 3678
Fragments arranged by size:
5438 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7076 8286 7076
Size: 1210 7974
BclI T'GATC_A
Cuts at: 1548 2454 5728 1548
Size: 906 3274 5004
Fragments arranged by size:
5004 3274 906
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7795 136
Size: 107 122 71 631 605 6123 1525
Fragments arranged by size:
6123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2272 4598 5764 9179 2272
Size: 2326 1166 3415 2277
Fragments arranged by size:
3415 2326 2277 1166
BlpI GC'TnA_GC
Cuts at: 6601 6601
Size: 9184
Bme1580I G_kGCm'C
Cuts at: 1790 5382 7245 8401 1790
Size: 3592 1863 1156 2573
Fragments arranged by size:
3592 2573 1863 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3797 6915 7873
Size: 416 53 83 399 24 2380 3118 958
Cuts at: 7873 442
Size: 1753
Fragments arranged by size:
3118 2380 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 3940 4852 2631
Size: 1309 912 6963
Fragments arranged by size:
6963 1309 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2297 2881 2908 3877 5820 6366 7844
Size: 845 213 584 27 969 1943 546 1478
Cuts at: 7844 1239
Size: 2579
Fragments arranged by size:
2579 1943 1478 969 845 584 546 213
27
Bpu10I CC'TnA_GC
Cuts at: 2567 5046 2567
Size: 2479 6705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2601 5293 7309 8174 8472 2159
Size: 442 2692 2016 865 298 2871
Fragments arranged by size:
2871 2692 2016 865 442 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3031 3445 7847 882
Size: 617 1532 414 4402 2219
Fragments arranged by size:
4402 2219 1532 617 414
BsaAI yAC'GTr
Cuts at: 493 1204 6975 493
Size: 711 5771 2702
Fragments arranged by size:
5771 2702 711
BsaBI GATnn'nnATC
Cuts at: 2911 3471 9178 2911
Size: 560 5707 2917
Fragments arranged by size:
5707 2917 560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 3911 6991 7373
Size: 53 83 186 936 771 1607 3080 382
Cuts at: 7373 8008 275
Size: 635 1451
Fragments arranged by size:
3080 1607 1451 936 771 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7617 8015 8289 8436 8847 7617
Size: 398 274 147 411 7954
Fragments arranged by size:
7954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3065 3095 1178
Size: 30 165 30 1662 30 7267
Fragments arranged by size:
7267 1662 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 3618 3973 4296 5180 1186
Size: 1418 1014 355 323 884 5190
Fragments arranged by size:
5190 1418 1014 884 355 323
BseYI C'CCAG_C
Cuts at: 3241 8387 8883 3241
Size: 5146 496 3542
Fragments arranged by size:
5146 3542 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3375 3620 1163
Size: 573 1639 245 6727
Fragments arranged by size:
6727 1639 573 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6634 7395 8423 1540
Size: 275 186 4633 761 1028 2301
Fragments arranged by size:
4633 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2867 5382 7245 7330 8401 2867
Size: 2515 1863 85 1071 3650
Fragments arranged by size:
3650 2515 1863 1071 85
BsmI GAATG_Cn'
Cuts at: 4390 5218 6247 6695 6788 4390
Size: 828 1029 448 93 6786
Fragments arranged by size:
6786 1029 828 448 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2848 2867 5160 5382 6503 7245 7330
Size: 399 1058 19 2293 222 1121 742 85
Cuts at: 7330 8401 1391
Size: 1071 2174
Fragments arranged by size:
2293 2174 1121 1071 1058 742 399 222
85 19
BspEI T'CCGG_A
Cuts at: 8847 8847
Size: 9184
BspHI T'CATG_A
Cuts at: 2076 5385 7073 8803 2076
Size: 3309 1688 1730 2457
Fragments arranged by size:
3309 2457 1730 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3318 4958 6539 844
Size: 2474 1640 1581 3489
Fragments arranged by size:
3489 2474 1640 1581
BsrBI CCG'CTC
Cuts at: 1394 1823 7071 1394
Size: 429 5248 3507
Fragments arranged by size:
5248 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2756 5120 7679 7853 65
Size: 1183 1508 2364 2559 174 1396
Fragments arranged by size:
2559 2364 1508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7828 8015 1700
Size: 110 6018 187 2869
Fragments arranged by size:
6018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4496 6121 6183 96
Size: 4400 1625 62 3097
Fragments arranged by size:
4400 3097 1625 62
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9184
BssSI C'ACGA_G
Cuts at: 3517 5679 7244 8256 3517
Size: 2162 1565 1012 4445
Fragments arranged by size:
4445 2162 1565 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9184
BstBI TT'CG_AA
Cuts at: 3691 7988 3691
Size: 4297 4887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9184
BstXI CCAn_nnnn'nTGG
Cuts at: 2040 2840 4357 5408 5977 6427 2040
Size: 800 1517 1051 569 450 4797
Fragments arranged by size:
4797 1517 1051 800 569 450
BstYI r'GATC_y
Cuts at: 1972 2272 2894 3896 4598 4709 4781 5764 6514
Size: 300 622 1002 702 111 72 983 750
Cuts at: 6514 6881 7266 7283 8735 8821 8833 9179 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1002 983 750 702 622 385
367 346 300 111 86 72 17 12
BstZ17I GTA'TAC
Cuts at: 3810 5372 3810
Size: 1562 7622
Bsu36I CC'TnA_GG
Cuts at: 3456 4428 5192 3456
Size: 972 764 7448
Fragments arranged by size:
7448 972 764
BtgI C'CryG_G
Cuts at: 513 1065 2004 6285 513
Size: 552 939 4281 3412
Fragments arranged by size:
4281 3412 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8445
Fragments arranged by size:
8445 683 56
BtsI GCAGTG_nn'
Cuts at: 6386 6700 7494 7514 6386
Size: 314 794 20 8056
Fragments arranged by size:
8056 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3585 3620 529
Size: 35 3021 35 6093
Fragments arranged by size:
6093 3021 35 35
DraI TTT'AAA
Cuts at: 6488 6488
Size: 9184
DraIII CAC_nnn'GTG
Cuts at: 4863 4863
Size: 9184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3916 8191 809
Size: 507 2600 4275 1802
Fragments arranged by size:
4275 2600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6041 6631 7520
Size: 54 1425 50 275 18 4211 590 889
Cuts at: 7520 9149 8
Size: 1629 43
Fragments arranged by size:
4211 1629 1425 889 590 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6631 1537
Size: 275 4819 4090
Fragments arranged by size:
4819 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2370 2469 3012 4356 4395 4581 5165
Size: 975 42 99 543 1344 39 186 584
Cuts at: 5165 5337 5928 6447 7114 1353
Size: 172 591 519 667 3423
Fragments arranged by size:
3423 1344 975 667 591 584 543 519
186 172 99 42 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7771 8145 8291 387
Size: 1064 138 6182 374 146 1280
Fragments arranged by size:
6182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2865 2865
Size: 9184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2297 2493 2694 2881 2908 3877
Size: 393 452 213 196 201 187 27 969
Cuts at: 3877 5820 5952 6366 7247 7844 8631 8899 1239
Size: 1943 132 414 881 597 787 268 1524
Fragments arranged by size:
1943 1524 969 881 787 597 452 414
393 268 213 201 196 187 132 27
EcoO109I rG'GnC_Cy
Cuts at: 6410 6470 6410
Size: 60 9124
EcoRI G'AATT_C
Cuts at: 2836 6493 6871 2836
Size: 3657 378 5149
Fragments arranged by size:
5149 3657 378
EcoRV GAT'ATC
Cuts at: 2649 2649
Size: 9184
FspI TGC'GCA
Cuts at: 6014 7690 6014
Size: 1676 7508
FspAI rTGC'GCAy
Cuts at: 6014 6014
Size: 9184
HaeII r_GCGC'y
Cuts at: 1678 2566 8331 1678
Size: 888 5765 2531
Fragments arranged by size:
5765 2531 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2849 2881 2987
Size: 32 135 32 692 32 553 32 106
Cuts at: 2987 3019 3134 3166 3182 3214 3851 3883 4013
Size: 32 115 32 16 32 637 32 130
Cuts at: 4013 4045 5623 5655 5713 5745 7825 7857 7899
Size: 32 1578 32 58 32 2080 32 42
Cuts at: 7899 7931 1373
Size: 32 2626
Fragments arranged by size:
2626 2080 1578 692 637 553 135 130
115 106 58 42 32 32 32 32
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 4308 4594 4978 6528 6774 669
Size: 988 281 2370 286 384 1550 246 3079
Fragments arranged by size:
3079 2370 1550 988 384 286 281 246
HindIII A'AGCT_T
Cuts at: 748 2178 2546 2806 748
Size: 1430 368 260 7126
Fragments arranged by size:
7126 1430 368 260
HpaI GTT'AAC
Cuts at: 6774 6774
Size: 9184
KpnI G_GTAC'C
Cuts at: 6509 6509
Size: 9184
MfeI C'AATT_G
Cuts at: 4989 6783 4989
Size: 1794 7390
MluI A'CGCG_T
Cuts at: 9060 9060
Size: 9184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3647 4715 4759 8298 8482 1305
Size: 2342 1068 44 3539 184 2007
Fragments arranged by size:
3539 2342 2007 1068 184 44
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6043 10
Size: 54 1425 343 4211 3151
Fragments arranged by size:
4211 3151 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3803 5468 6129 7142 7501 7660 518
Size: 648 2637 1665 661 1013 359 159 2042
Fragments arranged by size:
2637 2042 1665 1013 661 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4757 6600 7277 8425 8670 1606
Size: 3151 1843 677 1148 245 2120
Fragments arranged by size:
3151 2120 1843 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 6285 513
Size: 552 939 4281 3412
Fragments arranged by size:
4281 3412 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9184
NotI GC'GGCC_GC
Cuts at: 6631 6631
Size: 9184
NspI r_CATG'y
Cuts at: 1132 1229 5849 6542 8087 1132
Size: 97 4620 693 1545 2229
Fragments arranged by size:
4620 2229 1545 693 97
PasI CC'CwG_GG
Cuts at: 1348 4240 1348
Size: 2892 6292
PciI A'CATG_T
Cuts at: 8083 8083
Size: 9184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6039 6050 1108
Size: 4931 11 4242
Fragments arranged by size:
4931 4242 11
PfoI T'CCnGG_A
Cuts at: 3855 3905 5222 3855
Size: 50 1317 7817
Fragments arranged by size:
7817 1317 50
PmeI GTTT'AAAC
Cuts at: 6488 6488
Size: 9184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7253 7285 8766 8798 7253
Size: 32 1481 32 7639
Fragments arranged by size:
7639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6410 6410
Size: 9184
PshAI GACnn'nnGTC
Cuts at: 1613 4686 1613
Size: 3073 6111
PsiI TTA'TAA
Cuts at: 4964 6754 4964
Size: 1790 7394
PstI C_TGCA'G
Cuts at: 830 4342 4425 4923 4975 5055 6536 830
Size: 3512 83 498 52 80 1481 3478
Fragments arranged by size:
3512 3478 1481 498 83 80 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9184
PvuII CAG'CTG
Cuts at: 4757 4757
Size: 9184
SacI G_AGCT'C
Cuts at: 2867 2867
Size: 9184
SalI G'TCGA_C
Cuts at: 1936 6526 1936
Size: 4590 4594
SanDI GG'GwC_CC
Cuts at: 6410 6410
Size: 9184
SapI GCTCTTCn'nnn_
Cuts at: 2328 4395 5165 5928 2328
Size: 2067 770 763 5584
Fragments arranged by size:
5584 2067 770 763
SbfI CC_TGCA'GG
Cuts at: 6536 6536
Size: 9184
ScaI AGT'ACT
Cuts at: 4046 6103 7432 9144 4046
Size: 2057 1329 1712 4086
Fragments arranged by size:
4086 2057 1712 1329
SexAI A'CCwGG_T
Cuts at: 5400 5400
Size: 9184
SfcI C'TryA_G
Cuts at: 826 1045 3672 4267 4338 4421 4919 4971 5051
Size: 219 2627 595 71 83 498 52 80
Cuts at: 5051 6532 7667 8348 8539 826
Size: 1481 1135 681 191 1471
Fragments arranged by size:
2627 1481 1471 1135 681 595 498 219
191 83 80 71 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9184
SmaI CCC'GGG
Cuts at: 1872 6407 6511 1872
Size: 4535 104 4545
Fragments arranged by size:
4545 4535 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2598 2616 3376 5308 7288
Size: 197 926 195 460 18 760 1932 1980
Cuts at: 7288 8189 8451 820
Size: 901 262 1553
Fragments arranged by size:
1980 1932 1553 926 901 760 460 262
197 195 18
SnaBI TAC'GTA
Cuts at: 493 6975 493
Size: 6482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9184
SphI G_CATG'C
Cuts at: 1132 6542 1132
Size: 5410 3774
SspI AAT'ATT
Cuts at: 5 52 2227 7108 5
Size: 47 2175 4881 2081
Fragments arranged by size:
4881 2175 2081 47
StuI AGG'CCT
Cuts at: 1517 3704 1517
Size: 2187 6997
StyI C'CwwG_G
Cuts at: 513 1065 2004 3552 4684 6285 6623 513
Size: 552 939 1548 1132 1601 338 3074
Fragments arranged by size:
3074 1601 1548 1132 939 552 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2574 6475 7211 7228 7381 7566 8011
Size: 1255 994 3901 736 17 153 185 445
Cuts at: 8011 325
Size: 1498
Fragments arranged by size:
3901 1498 1255 994 736 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 4044 4396
Size: 274 80 33 51 1443 1514 553 352
Cuts at: 4396 4496 6101 6121 6183 7430 9142 96
Size: 100 1605 20 62 1247 1712 138
Fragments arranged by size:
1712 1605 1514 1443 1247 553 352 274
138 100 80 62 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2953 2985 3065 3097 5497 5529 9144 9176 2953
Size: 32 80 32 2400 32 3615 32 2961
Fragments arranged by size:
3615 2961 2400 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4638 1316
Size: 3322 5862
XbaI T'CTAG_A
Cuts at: 2731 6520 2731
Size: 3789 5395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5127 5127
Size: 9184
XhoI C'TCGA_G
Cuts at: 1943 2598 1943
Size: 655 8529
XmaI C'CCGG_G
Cuts at: 1870 6405 6509 1870
Size: 4535 104 4545
Fragments arranged by size:
4545 4535 104
XmnI GAAnn'nnTTC
Cuts at: 4885 7313 4885
Size: 2428 6756
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2305 3912 6992 276
Size: 53 83 186 936 771 1607 3080 2468
Fragments arranged by size:
3080 2468 1607 936 771 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI EcoNI FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SfiI SfoI SrfI SwaI