Restriction map for
pFN21AE1077
(Circular) (Six-base) MAPSORT of: pFN21AE1077.seq Check: 631 from: 1 to: 5389
pFN21AE1077 5389 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3199 278
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2732 1937
Size: 795 4594
Acc65I G'GTAC_C
Cuts at: 2710 2710
Size: 5389
AclI AA'CG_TT
Cuts at: 1563 3516 3889 1563
Size: 1953 373 3063
Fragments arranged by size:
3063 1953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2378 2560 3452 4836 5104 1632
Size: 563 183 182 892 1384 268 1917
Fragments arranged by size:
1917 1384 892 563 268 183 182
AfeI AGC'GCT
Cuts at: 2212 2672 2212
Size: 460 4929
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5192
AflIII A'CryG_T
Cuts at: 1203 2087 2140 4288 5265 1203
Size: 884 53 2148 977 1327
Fragments arranged by size:
2148 1327 977 884 53
AgeI A'CCGG_T
Cuts at: 2601 4220 2601
Size: 1619 3770
AhdI GACnn_n'nnGTC
Cuts at: 4118 4118
Size: 5389
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 3934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5389
AlwNI CAG_nnn'CTG
Cuts at: 3132 4704 3132
Size: 1572 3817
ApaLI G'TGCA_C
Cuts at: 3446 4602 3446
Size: 1156 4233
ApoI r'AATT_y
Cuts at: 1493 2293 2698 2924 3076 5185 1493
Size: 800 405 226 152 2109 1697
Fragments arranged by size:
2109 1697 800 405 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3943 160
Size: 872 2911 1606
Fragments arranged by size:
2911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2714 1870
Size: 73 300 471 4545
Fragments arranged by size:
4545 471 300 73
AvrII C'CTAG_G
Cuts at: 2074 2074
Size: 5389
BamHI G'GATC_C
Cuts at: 2719 3086 2719
Size: 367 5022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2218 2598 2710 4165
Size: 325 217 627 341 90 380 112 1455
Cuts at: 4165 618
Size: 1842
Fragments arranged by size:
1842 1455 627 380 341 325 217 112
90
BanII G_rGCy'C
Cuts at: 1391 2244 2708 1391
Size: 853 464 4072
Fragments arranged by size:
4072 853 464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3580 3614 1903
Size: 34 1643 34 3678
Fragments arranged by size:
3678 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2497 3281 4491 2497
Size: 784 1210 3395
Fragments arranged by size:
3395 1210 784
BclI T'GATC_A
Cuts at: 1548 2458 1548
Size: 910 4479
BfrBI ATG'CAT
Cuts at: 2387 2387
Size: 5389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4000 136
Size: 107 122 71 631 605 337 1991 1525
Fragments arranged by size:
1991 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5384 5384
Size: 5389
BlpI GC'TnA_GC
Cuts at: 2806 2806
Size: 5389
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3450 4606 1790
Size: 343 1317 1156 2573
Fragments arranged by size:
2573 1317 1156 343
BmgBI CAC'GTC
Cuts at: 1318 2143 1318
Size: 825 4564
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 4078 442
Size: 416 53 83 399 24 1703 958 1753
Fragments arranged by size:
1753 1703 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4049 1239
Size: 2810 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3514 4379 4677 3514
Size: 865 298 4226
Fragments arranged by size:
4226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2544 4052 882
Size: 617 1045 1508 2219
Fragments arranged by size:
2219 1508 1045 617
BsaAI yAC'GTr
Cuts at: 493 1204 2088 2323 3180 493
Size: 711 884 235 857 2702
Fragments arranged by size:
2702 884 857 711 235
BsaBI GATnn'nnATC
Cuts at: 5383 5383
Size: 5389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 3196 3578 4213
Size: 53 83 186 936 693 970 382 635
Cuts at: 4213 275
Size: 1451
Fragments arranged by size:
1451 970 936 693 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2313 2601 3822 4220 4494 4641 5052 2313
Size: 288 1221 398 274 147 411 2650
Fragments arranged by size:
2650 1221 411 398 288 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5164
Fragments arranged by size:
5164 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5389
BseYI C'CCAG_C
Cuts at: 4592 5088 4592
Size: 496 4893
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4816
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3600 4628 1540
Size: 275 186 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2264 2586 3450 3535 4606 2244
Size: 20 322 864 85 1071 3027
Fragments arranged by size:
3027 1071 864 322 85 20
BsmI GAATG_Cn'
Cuts at: 2900 2993 2900
Size: 93 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2244 2264 2586 2708 3450 3535
Size: 399 343 111 20 322 122 742 85
Cuts at: 3535 4606 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 322 122 111
85 20
BspEI T'CCGG_A
Cuts at: 5052 5052
Size: 5389
BspHI T'CATG_A
Cuts at: 3278 5008 3278
Size: 1730 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2744 844
Size: 1900 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 3276 1394
Size: 429 425 1028 3507
Fragments arranged by size:
3507 1028 429 425
BsrDI GCAATG_nn'
Cuts at: 65 1248 3884 4058 65
Size: 1183 2636 174 1396
Fragments arranged by size:
2636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2119 2595 2601 4033 4220 1700
Size: 110 243 66 476 6 1432 187 2869
Fragments arranged by size:
2869 1432 476 243 187 110 66 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5389
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5389
BssSI C'ACGA_G
Cuts at: 3449 4461 3449
Size: 1012 4377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5389
BstBI TT'CG_AA
Cuts at: 4193 4193
Size: 5389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5389
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5389
BstYI r'GATC_y
Cuts at: 1972 2310 2719 3086 3471 3488 4940 5026 5038
Size: 338 409 367 385 17 1452 86 12
Cuts at: 5038 5384 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 409 385 367 346 338 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2235 2278 513
Size: 552 939 231 43 3624
Fragments arranged by size:
3624 939 552 231 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4650
Fragments arranged by size:
4650 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 2905 3699 3719 2479
Size: 426 794 20 4149
Fragments arranged by size:
4149 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5354
DraI TTT'AAA
Cuts at: 2693 2693
Size: 5389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4396 809
Size: 507 3080 1802
Fragments arranged by size:
3080 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354
Size: 54 1425 50 275 18 1006 889 1629
Cuts at: 5354 8
Size: 43
Fragments arranged by size:
1629 1425 1006 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2836 1537
Size: 275 1024 4090
Fragments arranged by size:
4090 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 2637 3319 1353
Size: 1044 240 682 3423
Fragments arranged by size:
3423 1044 682 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 3976 4350 4496 387
Size: 1064 138 486 1901 374 146 1280
Fragments arranged by size:
1901 1280 1064 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2242
Size: 5389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2378 2560 3452 4049 4836 5104
Size: 393 563 183 182 892 597 787 268
Cuts at: 5104 1239
Size: 1524
Fragments arranged by size:
1524 892 787 597 563 393 268 183
182
EcoRI G'AATT_C
Cuts at: 2698 3076 2698
Size: 378 5011
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 5389
FspI TGC'GCA
Cuts at: 2040 3895 2040
Size: 1855 3534
HaeII r_GCGC'y
Cuts at: 1678 2214 2571 2596 2674 4536 1678
Size: 536 357 25 78 1862 2531
Fragments arranged by size:
2531 1862 536 357 78 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 2571 2603 4030
Size: 32 135 32 923 32 44 32 1427
Cuts at: 4030 4062 4104 4136 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1427 923 135 44 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2979 669
Size: 988 281 795 246 3079
Fragments arranged by size:
3079 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5389
HpaI GTT'AAC
Cuts at: 2979 2979
Size: 5389
KpnI G_GTAC'C
Cuts at: 2714 2714
Size: 5389
MfeI C'AATT_G
Cuts at: 2988 2988
Size: 5389
MluI A'CGCG_T
Cuts at: 5265 5265
Size: 5389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4687 1305
Size: 3198 184 2007
Fragments arranged by size:
3198 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3567
Fragments arranged by size:
3567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3347 3706 3865 518
Size: 648 1455 726 359 159 2042
Fragments arranged by size:
2042 1455 726 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2044 2805 3482 4630 4875 1606
Size: 414 24 761 677 1148 245 2120
Fragments arranged by size:
2120 1148 761 677 414 245 24
NaeI GCC'GGC
Cuts at: 2055 2597 2055
Size: 542 4847
NcoI C'CATG_G
Cuts at: 513 1065 2004 2278 513
Size: 552 939 274 3624
Fragments arranged by size:
3624 939 552 274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5389
NgoMIV G'CCGG_C
Cuts at: 2053 2595 2053
Size: 542 4847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5389
NotI GC'GGCC_GC
Cuts at: 2836 2836
Size: 5389
NsiI A_TGCA'T
Cuts at: 2389 2389
Size: 5389
NspI r_CATG'y
Cuts at: 1132 1229 2160 2747 4292 1132
Size: 97 931 587 1545 2229
Fragments arranged by size:
2229 1545 931 587 97
PasI CC'CwG_GG
Cuts at: 1348 2544 1348
Size: 1196 4193
PciI A'CATG_T
Cuts at: 4288 4288
Size: 5389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5389
PmeI GTTT'AAAC
Cuts at: 2693 2693
Size: 5389
PmlI CAC'GTG
Cuts at: 2088 2323 2088
Size: 235 5154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 4971 5003 3458
Size: 32 1481 32 3844
Fragments arranged by size:
3844 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5389
PsiI TTA'TAA
Cuts at: 2959 2959
Size: 5389
PspXI vC'TCGA_Gb
Cuts at: 2243 2243
Size: 5389
PstI C_TGCA'G
Cuts at: 830 2166 2741 830
Size: 1336 575 3478
Fragments arranged by size:
3478 1336 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5389
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5389
SacI G_AGCT'C
Cuts at: 2244 2244
Size: 5389
SalI G'TCGA_C
Cuts at: 1936 2731 1936
Size: 795 4594
SbfI CC_TGCA'GG
Cuts at: 2166 2741 2166
Size: 575 4814
ScaI AGT'ACT
Cuts at: 3637 5349 3637
Size: 1712 3677
SexAI A'CCwGG_T
Cuts at: 2685 2685
Size: 5389
SfcI C'TryA_G
Cuts at: 826 1045 2162 2737 3872 4553 4744 826
Size: 219 1117 575 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 681 575 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5389
SmaI CCC'GGG
Cuts at: 1872 2716 1872
Size: 844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 3493 4394 4656 820
Size: 197 926 300 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3180 493
Size: 2687 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5389
SphI G_CATG'C
Cuts at: 1132 2160 2747 1132
Size: 1028 587 3774
Fragments arranged by size:
3774 1028 587
SspI AAT'ATT
Cuts at: 5 52 3313 5
Size: 47 3261 2081
Fragments arranged by size:
3261 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5389
StyI C'CwwG_G
Cuts at: 513 1065 2004 2074 2278 2399 2828 513
Size: 552 939 70 204 121 429 3074
Fragments arranged by size:
3074 939 552 429 204 121 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3433 3586 3771 4216 325
Size: 1255 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3635 5347 96
Size: 274 80 33 51 1443 1658 1712 138
Fragments arranged by size:
1712 1658 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 5349 5381 2211
Size: 32 3106 32 2219
Fragments arranged by size:
3106 2219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5389
XbaI T'CTAG_A
Cuts at: 2725 2725
Size: 5389
XhoI C'TCGA_G
Cuts at: 1943 2243 1943
Size: 300 5089
XmaI C'CCGG_G
Cuts at: 1870 2714 1870
Size: 844 4545
XmnI GAAnn'nnTTC
Cuts at: 2297 3518 2297
Size: 1221 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3197 276
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PpuMI PspOMI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI