Restriction map for
pFN21AE1075
(Circular) (Six-base) MAPSORT of: pFN21AE1075.seq Check: 7951 from: 1 to: 7066
pFN21AE1075 7066 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4409 1937
Size: 2472 4594
Acc65I G'GTAC_C
Cuts at: 4387 4387
Size: 7066
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4140 5129 6513 6781 1632
Size: 2508 989 1384 268 1917
Fragments arranged by size:
2508 1917 1384 989 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6869
AflIII A'CryG_T
Cuts at: 1203 2855 5965 6942 1203
Size: 1652 3110 977 1327
Fragments arranged by size:
3110 1652 1327 977
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 2017 5795 2017
Size: 3778 3288
AleI CACnn'nnGTG
Cuts at: 1166 2993 1166
Size: 1827 5239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7066
AlwNI CAG_nnn'CTG
Cuts at: 3029 4809 6381 3029
Size: 1780 1572 3714
Fragments arranged by size:
3714 1780 1572
ApaLI G'TGCA_C
Cuts at: 3010 5123 6279 3010
Size: 2113 1156 3797
Fragments arranged by size:
3797 2113 1156
ApoI r'AATT_y
Cuts at: 1493 2658 3151 3229 3342 3608 3700 3794 3984
Size: 1165 493 78 113 266 92 94 190
Cuts at: 3984 4022 4213 4375 4601 4753 6862 1493
Size: 38 191 162 226 152 2109 1697
Fragments arranged by size:
2109 1697 1165 493 266 226 191 190
162 152 113 94 92 78 38
AseI AT'TA_AT
Cuts at: 160 1032 3315 3564 5620 160
Size: 872 2283 249 2056 1606
Fragments arranged by size:
2283 2056 1606 872 249
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 4391 1870
Size: 73 71 2377 4545
Fragments arranged by size:
4545 2377 73 71
AvrII C'CTAG_G
Cuts at: 3809 3809
Size: 7066
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4290 4323 4290
Size: 33 7033
BamHI G'GATC_C
Cuts at: 4396 4763 4396
Size: 367 6699
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4387 5842 618
Size: 325 217 627 2600 1455 1842
Fragments arranged by size:
2600 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4385 1391
Size: 2994 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2094 4321 928
Size: 971 195 2227 3673
Fragments arranged by size:
3673 2227 971 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5257 5291 1903
Size: 34 3320 34 3678
Fragments arranged by size:
3678 3320 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4958 6168 4958
Size: 1210 5856
BclI T'GATC_A
Cuts at: 1548 3672 3747 1548
Size: 2124 75 4867
Fragments arranged by size:
4867 2124 75
BfrBI ATG'CAT
Cuts at: 3497 3517 3497
Size: 20 7046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5677 136
Size: 107 122 71 631 605 4005 1525
Fragments arranged by size:
4005 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7061 7061
Size: 7066
BlpI GC'TnA_GC
Cuts at: 4483 4483
Size: 7066
Bme1580I G_kGCm'C
Cuts at: 1790 3014 5127 6283 1790
Size: 1224 2113 1156 2573
Fragments arranged by size:
2573 2113 1224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4229 4797 5755
Size: 416 53 83 399 24 2812 568 958
Cuts at: 5755 442
Size: 1753
Fragments arranged by size:
2812 1753 958 568 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2028 2990 3225 5726 1239
Size: 789 962 235 2501 2579
Fragments arranged by size:
2579 2501 962 789 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5191 6056 6354 5191
Size: 865 298 5903
Fragments arranged by size:
5903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2004 2116 5729 882
Size: 617 505 112 3613 2219
Fragments arranged by size:
3613 2219 617 505 112
BsaAI yAC'GTr
Cuts at: 493 1204 4857 493
Size: 711 3653 2702
Fragments arranged by size:
3653 2702 711
BsaBI GATnn'nnATC
Cuts at: 7060 7060
Size: 7066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4873 5255 5890 275
Size: 53 83 186 936 3340 382 635 1451
Fragments arranged by size:
3340 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5499 5897 6171 6318 6729 5499
Size: 398 274 147 411 5836
Fragments arranged by size:
5836 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6841
Fragments arranged by size:
6841 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7066
BseYI C'CCAG_C
Cuts at: 6269 6765 6269
Size: 496 6570
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3015 1163
Size: 573 1279 5214
Fragments arranged by size:
5214 1279 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4516 5277 6305 1540
Size: 275 186 2515 761 1028 2301
Fragments arranged by size:
2515 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3014 5127 5212 6283 3014
Size: 2113 85 1071 3797
Fragments arranged by size:
3797 2113 1071 85
BsmI GAATG_Cn'
Cuts at: 2941 4577 4670 2941
Size: 1636 93 5337
Fragments arranged by size:
5337 1636 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3014 4385 5127 5212 6283 1391
Size: 399 1224 1371 742 85 1071 2174
Fragments arranged by size:
2174 1371 1224 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6729 6729
Size: 7066
BspHI T'CATG_A
Cuts at: 4955 6685 4955
Size: 1730 5336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4421 844
Size: 3577 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4953 1394
Size: 429 3130 3507
Fragments arranged by size:
3507 3130 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4101 5561 5735 65
Size: 1183 2853 1460 174 1396
Fragments arranged by size:
2853 1460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5710 5897 1700
Size: 110 3900 187 2869
Fragments arranged by size:
3900 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4157 96
Size: 4061 3005
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 5126 6138 5126
Size: 1012 6054
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7066
BstBI TT'CG_AA
Cuts at: 5870 5870
Size: 7066
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7066
BstXI CCAn_nnnn'nTGG
Cuts at: 2412 2412
Size: 7066
BstYI r'GATC_y
Cuts at: 1972 4396 4763 5148 5165 6617 6703 6715 7061
Size: 2424 367 385 17 1452 86 12 346
Cuts at: 7061 1972
Size: 1977
Fragments arranged by size:
2424 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2055 2055
Size: 7066
BtgI C'CryG_G
Cuts at: 513 1065 2004 2650 3944 513
Size: 552 939 646 1294 3635
Fragments arranged by size:
3635 1294 939 646 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6327
Fragments arranged by size:
6327 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 4582 5376 5396 2293
Size: 2289 794 20 3963
Fragments arranged by size:
3963 2289 794 20
ClaI AT'CG_AT
Cuts at: 1554 2142 3268 1554
Size: 588 1126 5352
Fragments arranged by size:
5352 1126 588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7031
DraI TTT'AAA
Cuts at: 2241 2850 3057 3114 3261 3324 3705 3766 3990
Size: 609 207 57 147 63 381 61 224
Cuts at: 3990 4370 2241
Size: 380 4937
Fragments arranged by size:
4937 609 381 380 224 207 147 63
61 57
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4513 5402 7031
Size: 54 1425 50 275 18 2683 889 1629
Cuts at: 7031 8
Size: 43
Fragments arranged by size:
2683 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4513 1537
Size: 275 2701 4090
Fragments arranged by size:
4090 2701 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2925 3542 3810 4996 1353
Size: 1572 617 268 1186 3423
Fragments arranged by size:
3423 1572 1186 617 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5653 6027 6173 387
Size: 1064 138 4064 374 146 1280
Fragments arranged by size:
4064 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2990 3225 4140 5129 5726 6513
Size: 393 396 962 235 915 989 597 787
Cuts at: 6513 6781 1239
Size: 268 1524
Fragments arranged by size:
1524 989 962 915 787 597 396 393
268 235
EcoRI G'AATT_C
Cuts at: 4375 4753 4375
Size: 378 6688
FspI TGC'GCA
Cuts at: 5572 5572
Size: 7066
HaeII r_GCGC'y
Cuts at: 1678 2178 6213 1678
Size: 500 4035 2531
Fragments arranged by size:
4035 2531 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2275 2307 2951
Size: 32 135 32 431 32 240 32 644
Cuts at: 2951 2983 3281 3313 4178 4210 5707 5739 5781
Size: 32 298 32 865 32 1497 32 42
Cuts at: 5781 5813 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 865 644 431 298 240 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2070 3072 4320 4410 4656 669
Size: 988 281 132 1002 1248 90 246 3079
Fragments arranged by size:
3079 1248 1002 988 281 246 132 90
HindIII A'AGCT_T
Cuts at: 748 2604 3383 748
Size: 1856 779 4431
Fragments arranged by size:
4431 1856 779
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KpnI G_GTAC'C
Cuts at: 4391 4391
Size: 7066
MfeI C'AATT_G
Cuts at: 3275 4343 4665 3275
Size: 1068 322 5676
Fragments arranged by size:
5676 1068 322
MluI A'CGCG_T
Cuts at: 6942 6942
Size: 7066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 2393 6180 6364 1305
Size: 696 392 3787 184 2007
Fragments arranged by size:
3787 2007 696 392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5244
Fragments arranged by size:
5244 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 2993 5024 5383 5542 518
Size: 648 1372 455 2031 359 159 2042
Fragments arranged by size:
2042 2031 1372 648 455 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 4482 5159 6307 6552 1606
Size: 442 2434 677 1148 245 2120
Fragments arranged by size:
2434 2120 1148 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2650 3944 513
Size: 552 939 646 1294 3635
Fragments arranged by size:
3635 1294 939 646 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7066
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NsiI A_TGCA'T
Cuts at: 3499 3519 3499
Size: 20 7046
NspI r_CATG'y
Cuts at: 1132 1229 2537 3465 3497 4424 5969 1132
Size: 97 1308 928 32 927 1545 2229
Fragments arranged by size:
2229 1545 1308 928 927 97 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7066
PciI A'CATG_T
Cuts at: 5965 5965
Size: 7066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2553 4288 1108
Size: 1445 1735 3886
Fragments arranged by size:
3886 1735 1445
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5135 5167 6648 6680 5135
Size: 32 1481 32 5521
Fragments arranged by size:
5521 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7066
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2479 2511 2479
Size: 32 7034
PstI C_TGCA'G
Cuts at: 830 2300 4418 830
Size: 1470 2118 3478
Fragments arranged by size:
3478 2118 1470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 2048 2048
Size: 7066
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SapI GCTCTTCn'nnn_
Cuts at: 3810 3810
Size: 7066
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 3618 5314 7026 3618
Size: 1696 1712 3658
Fragments arranged by size:
3658 1712 1696
SfcI C'TryA_G
Cuts at: 826 1045 2296 2970 3075 3396 4293 4414 5549
Size: 219 1251 674 105 321 897 121 1135
Cuts at: 5549 6230 6421 826
Size: 681 191 1471
Fragments arranged by size:
1471 1251 1135 897 681 674 321 219
191 121 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 2016 4393 1872
Size: 144 2377 4545
Fragments arranged by size:
4545 2377 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5170 6071 6333 820
Size: 197 926 3227 901 262 1553
Fragments arranged by size:
3227 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4857 493
Size: 4364 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7066
SphI G_CATG'C
Cuts at: 1132 3497 4424 1132
Size: 2365 927 3774
Fragments arranged by size:
3774 2365 927
SspI AAT'ATT
Cuts at: 5 52 2731 2923 3140 3330 3652 4990 5
Size: 47 2679 192 217 190 322 1338 2081
Fragments arranged by size:
2679 2081 1338 322 217 192 190 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7066
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2650 3809 3944 4505 513
Size: 552 939 543 103 1159 135 561 3074
Fragments arranged by size:
3074 1159 939 561 552 543 135 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5093 5110 5263 5448 5893 325
Size: 1255 3513 17 153 185 445 1498
Fragments arranged by size:
3513 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3616 4157 5312
Size: 274 80 33 51 1443 1639 541 1155
Cuts at: 5312 7024 96
Size: 1712 138
Fragments arranged by size:
1712 1639 1443 1155 541 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3940 3972 7026 7058 3940
Size: 32 3054 32 3948
Fragments arranged by size:
3948 3054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7066
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3691 3691
Size: 7066
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7066
XmaI C'CCGG_G
Cuts at: 1870 2014 4391 1870
Size: 144 2377 4545
Fragments arranged by size:
4545 2377 144
XmnI GAAnn'nnTTC
Cuts at: 3231 5195 3231
Size: 1964 5102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI