Restriction map for
pFN21AE1071
(Circular) (Six-base) MAPSORT of: pFN21AE1071.seq Check: 9159 from: 1 to: 7000
pFN21AE1071 7000 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4343 1937
Size: 2406 4594
Acc65I G'GTAC_C
Cuts at: 2158 4321 2158
Size: 2163 4837
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 5063 6447 6715 1632
Size: 843 2588 1384 268 1917
Fragments arranged by size:
2588 1917 1384 843 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 5899 6876 1203
Size: 4696 977 1327
Fragments arranged by size:
4696 1327 977
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7000
AlwNI CAG_nnn'CTG
Cuts at: 3254 3379 4743 6315 3254
Size: 125 1364 1572 3939
Fragments arranged by size:
3939 1572 1364 125
ApaI G_GGCC'C
Cuts at: 2254 2254
Size: 7000
ApaLI G'TGCA_C
Cuts at: 2888 5057 6213 2888
Size: 2169 1156 3675
Fragments arranged by size:
3675 2169 1156
ApoI r'AATT_y
Cuts at: 1493 2379 2422 2787 4144 4309 4535 4687 6796
Size: 886 43 365 1357 165 226 152 2109
Cuts at: 6796 1493
Size: 1697
Fragments arranged by size:
2109 1697 1357 886 365 226 165 152
43
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2206 2314 4325 1870
Size: 73 263 108 2011 4545
Fragments arranged by size:
4545 2011 263 108 73
BamHI G'GATC_C
Cuts at: 4330 4697 4330
Size: 367 6633
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2158 4321 5776 618
Size: 325 217 627 371 2163 1455 1842
Fragments arranged by size:
2163 1842 1455 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2254 4319 1391
Size: 863 2065 4072
Fragments arranged by size:
4072 2065 863
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6029
BbvCI CC'TCA_GC
Cuts at: 2223 2223
Size: 7000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5191 5225 1903
Size: 34 3254 34 3678
Fragments arranged by size:
3678 3254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2590 4892 6102 2590
Size: 2302 1210 3488
Fragments arranged by size:
3488 2302 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5611 136
Size: 107 122 71 631 605 337 3602 1525
Fragments arranged by size:
3602 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2353 6995 2353
Size: 4642 2358
BlpI GC'TnA_GC
Cuts at: 4417 4417
Size: 7000
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2892 4107 5061 6217 1790
Size: 464 638 1215 954 1156 2573
Fragments arranged by size:
2573 1215 1156 954 638 464
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4731 5689 442
Size: 416 53 83 399 24 3314 958 1753
Fragments arranged by size:
3314 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2130 3765 5660 1239
Size: 870 21 1635 1895 2579
Fragments arranged by size:
2579 1895 1635 870 21
Bpu10I CC'TnA_GC
Cuts at: 2223 2282 2223
Size: 59 6941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3108 3383 5125 5990 6288 3108
Size: 275 1742 865 298 3820
Fragments arranged by size:
3820 1742 865 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2733 5663 882
Size: 617 1234 2930 2219
Fragments arranged by size:
2930 2219 1234 617
BsaAI yAC'GTr
Cuts at: 493 1204 4791 493
Size: 711 3587 2702
Fragments arranged by size:
3587 2702 711
BsaBI GATnn'nnATC
Cuts at: 2841 3015 6994 2841
Size: 174 3979 2847
Fragments arranged by size:
3979 2847 174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4807 5189 5824 275
Size: 53 83 186 936 3274 382 635 1451
Fragments arranged by size:
3274 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3763 3793 1178
Size: 30 165 30 2360 30 4385
Fragments arranged by size:
4385 2360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 2251 1186
Size: 851 214 5935
Fragments arranged by size:
5935 851 214
BseYI C'CCAG_C
Cuts at: 6203 6699 6203
Size: 496 6504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3999 1163
Size: 573 2263 4164
Fragments arranged by size:
4164 2263 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4450 5211 6239 1540
Size: 275 186 2449 761 1028 2301
Fragments arranged by size:
2449 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2892 5061 5146 6217 2892
Size: 2169 85 1071 3675
Fragments arranged by size:
3675 2169 1071 85
BsmI GAATG_Cn'
Cuts at: 2982 4511 4604 2982
Size: 1529 93 5378
Fragments arranged by size:
5378 1529 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2892 4107 4319 5061 5146 6217
Size: 399 464 638 1215 212 742 85 1071
Cuts at: 6217 1391
Size: 2174
Fragments arranged by size:
2174 1215 1071 742 638 464 399 212
85
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4355 844
Size: 3511 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 4887 1394
Size: 429 2041 1023 3507
Fragments arranged by size:
3507 2041 1023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2516 2644 2846 3736 5495 5669 65
Size: 1183 1268 128 202 890 1759 174 1396
Fragments arranged by size:
1759 1396 1268 1183 890 202 174 128
BsrFI r'CCGG_y
Cuts at: 1700 1810 5644 5831 1700
Size: 110 3834 187 2869
Fragments arranged by size:
3834 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 5060 6072 5060
Size: 1012 5988
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7000
BstBI TT'CG_AA
Cuts at: 5804 5804
Size: 7000
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7000
BstXI CCAn_nnnn'nTGG
Cuts at: 3881 3881
Size: 7000
BstYI r'GATC_y
Cuts at: 1972 2353 2953 3892 4330 4697 5082 5099 6551
Size: 381 600 939 438 367 385 17 1452
Cuts at: 6551 6637 6649 6995 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 939 600 438 385 381 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2198 3620 513
Size: 552 939 194 1422 3893
Fragments arranged by size:
3893 1422 939 552 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 4107 4516 5310 5330 4107
Size: 409 794 20 5777
Fragments arranged by size:
5777 794 409 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 2877 4304 2877
Size: 1427 5573
DraIII CAC_nnn'GTG
Cuts at: 2233 2233
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3623 4447 5336
Size: 54 1425 50 275 18 1793 824 889
Cuts at: 5336 6965 8
Size: 1629 43
Fragments arranged by size:
1793 1629 1425 889 824 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 3384 3710 3930 4930 1353
Size: 900 1131 326 220 1000 3423
Fragments arranged by size:
3423 1131 1000 900 326 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3682 5587 5961 6107 387
Size: 1064 138 2093 1905 374 146 1280
Fragments arranged by size:
2093 1905 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2130 2475 3765 5063 5660 6447
Size: 393 477 21 345 1290 1298 597 787
Cuts at: 6447 6715 1239
Size: 268 1524
Fragments arranged by size:
1524 1298 1290 787 597 477 393 345
268 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2258
Size: 7000
EcoO109I rG'GnC_Cy
Cuts at: 2136 2251 2136
Size: 115 6885
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2372 2372
Size: 7000
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 4256 6147 1678
Size: 2578 1891 2531
Fragments arranged by size:
2578 2531 1891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2945 2977 3137 3169 3178
Size: 32 135 32 1373 32 160 32 9
Cuts at: 3178 3210 3460 3492 5641 5673 5715 5747 1373
Size: 32 250 32 2149 32 42 32 2626
Fragments arranged by size:
2626 2149 1373 250 160 135 42 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 3226 3771 4344 4590 669
Size: 988 281 543 745 545 573 246 3079
Fragments arranged by size:
3079 988 745 573 545 543 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 2481 4590 2481
Size: 2109 4891
KpnI G_GTAC'C
Cuts at: 2162 4325 2162
Size: 2163 4837
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2276 6114 6298 1305
Size: 971 3838 184 2007
Fragments arranged by size:
3838 2007 971 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3625 10
Size: 54 1425 343 1793 3385
Fragments arranged by size:
3385 1793 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2869 4958 5317 5476 518
Size: 648 1703 2089 359 159 2042
Fragments arranged by size:
2089 2042 1703 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2322 4416 5093 6241 6486 1606
Size: 716 2094 677 1148 245 2120
Fragments arranged by size:
2120 2094 1148 716 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5509
Fragments arranged by size:
5509 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 2922 4358 5903 1132
Size: 97 1693 1436 1545 2229
Fragments arranged by size:
2229 1693 1545 1436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7000
PciI A'CATG_T
Cuts at: 5899 5899
Size: 7000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2665 1108
Size: 1557 5443
PfoI T'CCnGG_A
Cuts at: 3895 3895
Size: 7000
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3703 3735 5069 5101 6582 6614 3703
Size: 32 1334 32 1481 32 4089
Fragments arranged by size:
4089 1481 1334 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2136
Size: 7000
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PspOMI G'GGCC_C
Cuts at: 2250 2250
Size: 7000
PstI C_TGCA'G
Cuts at: 830 2220 2475 4352 830
Size: 1390 255 1877 3478
Fragments arranged by size:
3478 1877 1390 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SanDI GG'GwC_CC
Cuts at: 2136 2136
Size: 7000
SapI GCTCTTCn'nnn_
Cuts at: 3930 3930
Size: 7000
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 2903 5248 6960 2903
Size: 2345 1712 2943
Fragments arranged by size:
2943 2345 1712
SfcI C'TryA_G
Cuts at: 826 1045 2216 2471 4348 5483 6164 6355 826
Size: 219 1171 255 1877 1135 681 191 1471
Fragments arranged by size:
1877 1471 1171 1135 681 255 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 2208 2316 4327 1872
Size: 336 108 2011 4545
Fragments arranged by size:
4545 2011 336 108
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3123 3362 5104 6005 6267 820
Size: 197 926 1180 239 1742 901 262 1553
Fragments arranged by size:
1742 1553 1180 926 901 262 239 197
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SspI AAT'ATT
Cuts at: 5 52 2911 3692 4924 5
Size: 47 2859 781 1232 2081
Fragments arranged by size:
2859 2081 1232 781 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2004 4275 4439 513
Size: 552 939 2271 164 3074
Fragments arranged by size:
3074 2271 939 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2360 2798 4160 5027 5044 5197
Size: 1255 582 198 438 1362 867 17 153
Cuts at: 5197 5382 5827 325
Size: 185 445 1498
Fragments arranged by size:
1498 1362 1255 867 582 445 438 198
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 5246 6958
Size: 274 80 33 51 1443 924 2345 1712
Cuts at: 6958 96
Size: 138
Fragments arranged by size:
2345 1712 1443 924 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6960 6992 6960
Size: 32 6968
Tth111I GACn'n_nGTC
Cuts at: 1316 3107 1316
Size: 1791 5209
XbaI T'CTAG_A
Cuts at: 4336 4336
Size: 7000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4114 4114
Size: 7000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 2206 2314 4325 1870
Size: 336 108 2011 4545
Fragments arranged by size:
4545 2011 336 108
XmnI GAAnn'nnTTC
Cuts at: 4146 5129 4146
Size: 983 6017
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI