Restriction map for
pFN21AE1067
(Circular) (Six-base) MAPSORT of: pFN21AE1067.seq Check: 2729 from: 1 to: 6223
pFN21AE1067 6223 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3058 3058
Size: 6223
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2374 2410 2975 3107 4286 5670 5938
Size: 647 95 36 565 132 1179 1384 268
Cuts at: 5938 1632
Size: 1917
Fragments arranged by size:
1917 1384 1179 647 565 268 132 95
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 3439 4952 3439
Size: 1513 4710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2556 1184
Size: 1372 4851
AlwNI CAG_nnn'CTG
Cuts at: 3490 3966 5538 3490
Size: 476 1572 4175
Fragments arranged by size:
4175 1572 476
ApaLI G'TGCA_C
Cuts at: 4280 5436 4280
Size: 1156 5067
ApoI r'AATT_y
Cuts at: 1493 2699 3532 3758 3910 6019 1493
Size: 1206 833 226 152 2109 1697
Fragments arranged by size:
2109 1697 1206 833 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 3311 3548 1870
Size: 73 460 908 237 4545
Fragments arranged by size:
4545 908 460 237 73
BamHI G'GATC_C
Cuts at: 2055 3553 3920 2055
Size: 1498 367 4358
Fragments arranged by size:
4358 1498 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 3544 4999 618
Size: 325 217 627 624 1133 1455 1842
Fragments arranged by size:
1842 1455 1133 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 2787 3116 3157 3366 3542 1391
Size: 1396 329 41 209 176 4072
Fragments arranged by size:
4072 1396 329 209 176 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5252
BbvCI CC'TCA_GC
Cuts at: 2973 2973
Size: 6223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4414 4448 1903
Size: 34 50 34 2393 34 3678
Fragments arranged by size:
3678 2393 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2169 4115 5325 2169
Size: 1946 1210 3067
Fragments arranged by size:
3067 1946 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2624 2633 4834
Size: 107 122 71 631 605 952 9 2201
Cuts at: 4834 136
Size: 1525
Fragments arranged by size:
2201 1525 952 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2385 6218 2385
Size: 3833 2390
BlpI GC'TnA_GC
Cuts at: 2516 3640 2516
Size: 1124 5099
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2852 4284 5440 1790
Size: 256 806 1432 1156 2573
Fragments arranged by size:
2573 1432 1156 806 256
BmgBI CAC'GTC
Cuts at: 1318 2128 2304 1318
Size: 810 176 5237
Fragments arranged by size:
5237 810 176
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3500 3954 4912
Size: 416 53 83 399 24 2083 454 958
Cuts at: 4912 442
Size: 1753
Fragments arranged by size:
2083 1753 958 454 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 3451 2644
Size: 807 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2634 2823 2876 2888 3011 3026 3152 3308
Size: 1395 189 53 12 123 15 126 156
Cuts at: 3308 3380 4883 1239
Size: 72 1503 2579
Fragments arranged by size:
2579 1503 1395 189 156 126 123 72
53 15 12
Bpu10I CC'TnA_GC
Cuts at: 2587 2973 3186 2587
Size: 386 213 5624
Fragments arranged by size:
5624 386 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4348 5213 5511 4348
Size: 865 298 5060
Fragments arranged by size:
5060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2586 4886 882
Size: 617 1087 2300 2219
Fragments arranged by size:
2300 2219 1087 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 4014 493
Size: 711 909 1901 2702
Fragments arranged by size:
2702 1901 909 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 4030 4412 5047
Size: 53 83 186 936 543 1954 382 635
Cuts at: 5047 275
Size: 1451
Fragments arranged by size:
1954 1451 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3149 4656 5054 5328 5475 5886 3149
Size: 1507 398 274 147 411 3486
Fragments arranged by size:
3486 1507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2235 2265 2744 2774 3389
Size: 30 165 30 832 30 479 30 615
Cuts at: 3389 3419 1178
Size: 30 3982
Fragments arranged by size:
3982 832 615 479 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 2879 2891 3440 3464 1186
Size: 1474 219 12 549 24 3945
Fragments arranged by size:
3945 1474 549 219 24 12
BseYI C'CCAG_C
Cuts at: 3214 3328 5426 5922 3214
Size: 114 2098 496 3515
Fragments arranged by size:
3515 2098 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5650
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3673 4434 5462 1540
Size: 275 186 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3168 3366 4284 4369 5440 3168
Size: 198 918 85 1071 3951
Fragments arranged by size:
3951 1071 918 198 85
BsmI GAATG_Cn'
Cuts at: 2545 3202 3734 3827 2545
Size: 657 532 93 4941
Fragments arranged by size:
4941 657 532 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2787 2852 3116 3157 3168 3366
Size: 399 256 741 65 264 41 11 198
Cuts at: 3366 3542 4284 4369 5440 1391
Size: 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 399 264 256 198
176 85 65 41 11
BspEI T'CCGG_A
Cuts at: 3149 5886 3149
Size: 2737 3486
BspHI T'CATG_A
Cuts at: 2365 4112 5842 2365
Size: 1747 1730 2746
Fragments arranged by size:
2746 1747 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 3058 3207 3578 844
Size: 1214 1000 149 371 3489
Fragments arranged by size:
3489 1214 1000 371 149
BsrBI CCG'CTC
Cuts at: 1394 1823 3147 4110 1394
Size: 429 1324 963 3507
Fragments arranged by size:
3507 1324 963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4718 4892 65
Size: 1183 3470 174 1396
Fragments arranged by size:
3470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4867 5054 1700
Size: 110 3057 187 2869
Fragments arranged by size:
3057 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 3436 4283 5295 3436
Size: 847 1012 4364
Fragments arranged by size:
4364 1012 847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 4967
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstXI CCAn_nnnn'nTGG
Cuts at: 2558 2612 2558
Size: 54 6169
BstYI r'GATC_y
Cuts at: 1972 2055 2349 2385 3553 3920 4305 4322 5774
Size: 83 294 36 1168 367 385 17 1452
Cuts at: 5774 5860 5872 6218 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1168 385 367 346 294 86
83 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 3259 3259
Size: 6223
BtgI C'CryG_G
Cuts at: 513 1065 2631 3108 3519 513
Size: 552 1566 477 411 3217
Fragments arranged by size:
3217 1566 552 477 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3373 487
Size: 683 56 2147 3337
Fragments arranged by size:
3337 2147 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 3739 4533 4553 2637
Size: 1102 794 20 4307
Fragments arranged by size:
4307 1102 794 20
ClaI AT'CG_AT
Cuts at: 1554 2658 1554
Size: 1104 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 2191 2443 3527 2191
Size: 252 1084 4887
Fragments arranged by size:
4887 1084 252
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2863 5230 809
Size: 507 1547 2367 1802
Fragments arranged by size:
2367 1802 1547 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 2634 3670
Size: 54 1425 50 275 18 718 86 1036
Cuts at: 3670 4559 6188 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1036 889 718 275 86 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2450 4153 1353
Size: 1097 1703 3423
Fragments arranged by size:
3423 1703 1097
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3297 4810 5184 5330 387
Size: 1064 138 1708 1513 374 146 1280
Fragments arranged by size:
1708 1513 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3364 3364
Size: 6223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2374 2410 2634 2823 2876 2888
Size: 393 647 95 36 224 189 53 12
Cuts at: 2888 2975 3011 3026 3107 3152 3308 3380 4286
Size: 87 36 15 81 45 156 72 906
Cuts at: 4286 4883 5670 5938 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 906 787 647 597 393 268 224
189 156 95 87 81 72 53 45
36 36 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2803 3262 2803
Size: 459 5764
EcoO109I rG'GnC_Cy
Cuts at: 2335 2625 2671 3196 2335
Size: 290 46 525 5362
Fragments arranged by size:
5362 525 290 46
EcoRI G'AATT_C
Cuts at: 2699 3532 3910 2699
Size: 833 378 5012
Fragments arranged by size:
5012 833 378
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 3347 5370 1678
Size: 1669 2023 2531
Fragments arranged by size:
2531 2023 1669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3389 3421 3425 3457 4864
Size: 32 135 32 1817 32 4 32 1407
Cuts at: 4864 4896 4938 4970 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1817 1407 135 42 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3567 3813 669
Size: 988 281 1629 246 3079
Fragments arranged by size:
3079 1629 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2273 748
Size: 1525 4698
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3432 5337 5521 1305
Size: 2127 1905 184 2007
Fragments arranged by size:
2127 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 10
Size: 54 1425 343 718 3683
Fragments arranged by size:
3683 1425 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4181 4540 4699 518
Size: 648 3015 359 159 2042
Fragments arranged by size:
3015 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2863 3214 3639 4316 5464 5709 1606
Size: 1257 351 425 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 677 425 351 245
NcoI C'CATG_G
Cuts at: 513 1065 3108 513
Size: 552 2043 3628
Fragments arranged by size:
3628 2043 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 3040 3581 5126 1132
Size: 97 1811 541 1545 2229
Fragments arranged by size:
2229 1811 1545 541 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6223
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6223
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 6223
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2671 3196 2335
Size: 336 525 5362
Fragments arranged by size:
5362 525 336
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PstI C_TGCA'G
Cuts at: 830 2526 2644 3027 3480 3575 830
Size: 1696 118 383 453 95 3478
Fragments arranged by size:
3478 1696 453 383 118 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
SacI G_AGCT'C
Cuts at: 3366 3366
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SapI GCTCTTCn'nnn_
Cuts at: 2450 2450
Size: 6223
SbfI CC_TGCA'GG
Cuts at: 3575 3575
Size: 6223
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 2344 2522 2640 2730 3023 3476 3571
Size: 219 1299 178 118 90 293 453 95
Cuts at: 3571 4706 5387 5578 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1299 1135 681 453 293 219 191
178 118 95 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 2633 2633
Size: 6223
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2403 4327 5228 5490 820
Size: 197 926 460 1924 901 262 1553
Fragments arranged by size:
1924 1553 926 901 460 262 197
SnaBI TAC'GTA
Cuts at: 493 2113 4014 493
Size: 1620 1901 2702
Fragments arranged by size:
2702 1901 1620
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 2844 1517
Size: 978 349 4896
Fragments arranged by size:
4896 978 349
StyI C'CwwG_G
Cuts at: 513 1065 2132 2763 3108 3662 513
Size: 552 1067 631 345 554 3074
Fragments arranged by size:
3074 1067 631 554 552 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 4250 4267 4420 4605 5050 325
Size: 1255 1238 1432 17 153 185 445 1498
Fragments arranged by size:
1498 1432 1255 1238 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 4469 6181
Size: 274 80 33 51 1443 45 2447 1712
Cuts at: 6181 96
Size: 138
Fragments arranged by size:
2447 1712 1443 274 138 80 51 45
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 2288 2320 2744 2776 6183 6215 2235
Size: 32 21 32 424 32 3407 32 2243
Fragments arranged by size:
3407 2243 424 32 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6223
XbaI T'CTAG_A
Cuts at: 2722 3559 2722
Size: 837 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2146
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 2403 1943
Size: 460 5763
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 2387 4352 2387
Size: 1965 4258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfoI SrfI SwaI