Restriction map for
pFN21AE1065
(Circular) (Six-base) MAPSORT of: pFN21AE1065.seq Check: 56 from: 1 to: 7669
pFN21AE1065 7669 bp
With 182 enzymes: SgfI *
February 27, 2009 15:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2972 2972
Size: 7669
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5479 278
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2100 5012 1937
Size: 163 2912 4594
Fragments arranged by size:
4594 2912 163
Acc65I G'GTAC_C
Cuts at: 4990 4990
Size: 7669
AclI AA'CG_TT
Cuts at: 1563 5796 6169 1563
Size: 4233 373 3063
Fragments arranged by size:
4233 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5732 7116 7384 1632
Size: 4100 1384 268 1917
Fragments arranged by size:
4100 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7472
AflIII A'CryG_T
Cuts at: 1203 2678 4041 6568 7545 1203
Size: 1475 1363 2527 977 1327
Fragments arranged by size:
2527 1475 1363 1327 977
AgeI A'CCGG_T
Cuts at: 6500 6500
Size: 7669
AhdI GACnn_n'nnGTC
Cuts at: 6398 6398
Size: 7669
AleI CACnn'nnGTG
Cuts at: 1166 2652 2789 3921 1166
Size: 1486 137 1132 4914
Fragments arranged by size:
4914 1486 1132 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3404 1184
Size: 2220 5449
AlwNI CAG_nnn'CTG
Cuts at: 2786 3043 3547 3736 3806 5412 6984 2786
Size: 257 504 189 70 1606 1572 3471
Fragments arranged by size:
3471 1606 1572 504 257 189 70
ApaI G_GGCC'C
Cuts at: 3256 3426 3256
Size: 170 7499
ApaLI G'TGCA_C
Cuts at: 4226 5726 6882 4226
Size: 1500 1156 5013
Fragments arranged by size:
5013 1500 1156
ApoI r'AATT_y
Cuts at: 1493 4809 4978 5204 5356 7465 1493
Size: 3316 169 226 152 2109 1697
Fragments arranged by size:
3316 2109 1697 226 169 152
AseI AT'TA_AT
Cuts at: 160 1032 6223 160
Size: 872 5191 1606
Fragments arranged by size:
5191 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 3025 3172 3249 3419 4139 4301
Size: 73 344 738 147 77 170 720 162
Cuts at: 4301 4994 1870
Size: 693 4545
Fragments arranged by size:
4545 738 720 693 344 170 162 147
77 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2287 2320 2287
Size: 33 7636
BamHI G'GATC_C
Cuts at: 4509 4671 4999 5366 4509
Size: 162 328 367 6812
Fragments arranged by size:
6812 367 328 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3404 3606 3911 4031 4193
Size: 325 217 627 1617 202 305 120 162
Cuts at: 4193 4289 4779 4990 6445 618
Size: 96 490 211 1455 1842
Fragments arranged by size:
1842 1617 1455 627 490 325 305 217
211 202 162 120 96
BanII G_rGCy'C
Cuts at: 1391 2088 3256 3426 3933 4140 4988 1391
Size: 697 1168 170 507 207 848 4072
Fragments arranged by size:
4072 1168 848 697 507 207 170
BbeI G_GCGC'C
Cuts at: 3610 4783 3610
Size: 1173 6496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2229 2843 928
Size: 971 330 614 5754
Fragments arranged by size:
5754 971 614 330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2710 2744 2784 2818 3751 3785 3901
Size: 34 773 34 40 34 933 34 116
Cuts at: 3901 3935 4336 4370 5860 5894 1903
Size: 34 401 34 1490 34 3678
Fragments arranged by size:
3678 1490 933 773 401 116 40 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5561 6771 5561
Size: 1210 6459
BclI T'GATC_A
Cuts at: 1548 4830 1548
Size: 3282 4387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 2934 4119
Size: 107 122 71 631 605 767 495 1185
Cuts at: 4119 4287 6280 136
Size: 168 1993 1525
Fragments arranged by size:
1993 1525 1185 767 631 605 495 168
122 107 71
BglII A'GATC_T
Cuts at: 2394 4530 7664 2394
Size: 2136 3134 2399
Fragments arranged by size:
3134 2399 2136
BlpI GC'TnA_GC
Cuts at: 5086 5086
Size: 7669
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2126 2527 3256 3426 4004 4036 4124
Size: 267 69 401 729 170 578 32 88
Cuts at: 4124 4230 4722 5730 6886 1790
Size: 106 492 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 729 578 492 401 267
170 106 88 69 32
BmgBI CAC'GTC
Cuts at: 1318 2104 2736 4596 4849 1318
Size: 786 632 1860 253 4138
Fragments arranged by size:
4138 1860 786 632 253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5400 6358 442
Size: 416 53 83 399 24 3983 958 1753
Fragments arranged by size:
3983 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4605 4605
Size: 7669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3863 4053 6329 1239
Size: 2624 190 2276 2579
Fragments arranged by size:
2624 2579 2276 190
Bpu10I CC'TnA_GC
Cuts at: 3353 4134 3353
Size: 781 6888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3566 5794 6659 6957 3566
Size: 2228 865 298 4278
Fragments arranged by size:
4278 2228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3524 3802 4607 6332 882
Size: 617 2025 278 805 1725 2219
Fragments arranged by size:
2219 2025 1725 805 617 278
BsaAI yAC'GTr
Cuts at: 493 1204 2679 2724 4125 5460 493
Size: 711 1475 45 1401 1335 2702
Fragments arranged by size:
2702 1475 1401 1335 711 45
BsaBI GATnn'nnATC
Cuts at: 4514 7663 4514
Size: 3149 4520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2543 3607 4780 5476
Size: 53 83 186 936 1010 1064 1173 696
Cuts at: 5476 5858 6493 275
Size: 382 635 1451
Fragments arranged by size:
1451 1173 1064 1010 936 696 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2763 6102 6500 6774 6921 7332 2763
Size: 3339 398 274 147 411 3100
Fragments arranged by size:
3339 3100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2969 2999 1178
Size: 30 165 30 1566 30 5848
Fragments arranged by size:
5848 1566 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3583 1186
Size: 2397 5272
BseYI C'CCAG_C
Cuts at: 3255 3425 4004 4459 6872 7368 3255
Size: 170 579 455 2413 496 3556
Fragments arranged by size:
3556 2413 579 496 455 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2593 3121 1163
Size: 573 857 528 5711
Fragments arranged by size:
5711 857 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2719 2764 3775 4086 5119
Size: 275 186 18 700 45 1011 311 1033
Cuts at: 5119 5880 6908 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1033 1028 1011 761 700 311 275
186 45 18
BsiHKAI G_wGCw'C
Cuts at: 2117 2987 3933 4230 5730 5815 6886 2117
Size: 870 946 297 1500 85 1071 2900
Fragments arranged by size:
2900 1500 1071 946 870 297 85
BsmI GAATG_Cn'
Cuts at: 4478 5180 5273 4478
Size: 702 93 6874
Fragments arranged by size:
6874 702 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2534 2726 2534
Size: 192 7477
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2088 2117 2126 2527 2987 3256
Size: 399 267 31 29 9 401 460 269
Cuts at: 3256 3426 3933 4004 4036 4124 4140 4230 4722
Size: 170 507 71 32 88 16 90 492
Cuts at: 4722 4988 5730 5815 6886 1391
Size: 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 492 460 401 399
269 267 266 170 90 88 85 71
32 31 29 16 9
BspEI T'CCGG_A
Cuts at: 7332 7332
Size: 7669
BspHI T'CATG_A
Cuts at: 4572 5558 7288 4572
Size: 986 1730 4953
Fragments arranged by size:
4953 1730 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 2477 2972 4625 5024 844
Size: 1471 162 495 1653 399 3489
Fragments arranged by size:
3489 1653 1471 495 399 162
BsrBI CCG'CTC
Cuts at: 1394 1823 2481 2995 4656 5556 1394
Size: 429 658 514 1661 900 3507
Fragments arranged by size:
3507 1661 900 658 514 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6164 6338 65
Size: 1183 4916 174 1396
Fragments arranged by size:
4916 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3774 4148 4421 6313 6500 1700
Size: 110 1964 374 273 1892 187 2869
Fragments arranged by size:
2869 1964 1892 374 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7669
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7669
BssSI C'ACGA_G
Cuts at: 2270 3519 5729 6741 2270
Size: 1249 2210 1012 3198
Fragments arranged by size:
3198 2210 1249 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3547 3999 4152 4278 794
Size: 2753 452 153 126 4185
Fragments arranged by size:
4185 2753 452 153 126
BstBI TT'CG_AA
Cuts at: 6473 6473
Size: 7669
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7669
BstXI CCAn_nnnn'nTGG
Cuts at: 3841 3923 3841
Size: 82 7587
BstYI r'GATC_y
Cuts at: 1972 2394 3477 4509 4530 4671 4999 5366 5751
Size: 422 1083 1032 21 141 328 367 385
Cuts at: 5751 5768 7220 7306 7318 7664 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1083 1032 422 385 367 346
328 141 86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 2515 2904 3052 3610 4116
Size: 552 939 405 106 389 148 558 506
Cuts at: 4116 4910 513
Size: 794 3272
Fragments arranged by size:
3272 939 794 558 552 506 405 389
148 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2809 4471 4575 4624 4788 487
Size: 683 56 1583 1662 104 49 164 3368
Fragments arranged by size:
3368 1662 1583 683 164 104 56 49
BtsI GCAGTG_nn'
Cuts at: 2222 2819 3079 3556 4228 4318 4378 4393 5185
Size: 597 260 477 672 90 60 15 792
Cuts at: 5185 5979 5999 2222
Size: 794 20 3892
Fragments arranged by size:
3892 794 792 672 597 477 260 90
60 20 15
ClaI AT'CG_AT
Cuts at: 1554 2142 1554
Size: 588 7081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2362 2397 529
Size: 35 1798 35 5801
Fragments arranged by size:
5801 1798 35 35
DraI TTT'AAA
Cuts at: 4973 4973
Size: 7669
DraIII CAC_nnn'GTG
Cuts at: 3999 3999
Size: 7669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6676 809
Size: 507 5360 1802
Fragments arranged by size:
5360 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2070 3613
Size: 54 1425 50 275 18 186 54 1543
Cuts at: 3613 3772 3934 4083 4240 4309 5116 6005 7634
Size: 159 162 149 157 69 807 889 1629
Cuts at: 7634 8
Size: 43
Fragments arranged by size:
1629 1543 1425 889 807 275 186 162
159 157 149 69 54 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3772 4083 5116 1537
Size: 275 204 1756 311 1033 4090
Fragments arranged by size:
4090 1756 1033 311 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3326 3596 3675 4574 4847 5599 1353
Size: 1973 270 79 899 273 752 3423
Fragments arranged by size:
3423 1973 899 752 273 270 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6256 6630 6776 387
Size: 1064 138 4667 374 146 1280
Fragments arranged by size:
4667 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3931 3931
Size: 7669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3863 4053 5732 6329 7116 7384 1239
Size: 393 2231 190 1679 597 787 268 1524
Fragments arranged by size:
2231 1679 1524 787 597 393 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 4630 4630
Size: 7669
EcoO109I rG'GnC_Cy
Cuts at: 2672 4837 2672
Size: 2165 5504
EcoRI G'AATT_C
Cuts at: 4978 5356 4978
Size: 378 7291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3487 4858 3487
Size: 1371 6298
FspI TGC'GCA
Cuts at: 2740 3761 6175 2740
Size: 1021 2414 4234
Fragments arranged by size:
4234 2414 1021
HaeII r_GCGC'y
Cuts at: 1678 3389 3610 3842 4783 6816 1678
Size: 1711 221 232 941 2033 2531
Fragments arranged by size:
2531 2033 1711 941 232 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2969 3001 6310
Size: 32 135 32 527 32 838 32 3309
Cuts at: 6310 6342 6384 6416 1373
Size: 32 42 32 2626
Fragments arranged by size:
3309 2626 838 527 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 2869 3139 5013 5259 669
Size: 988 281 163 768 270 1874 246 3079
Fragments arranged by size:
3079 1874 988 768 281 270 246 163
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7669
HpaI GTT'AAC
Cuts at: 5259 5259
Size: 7669
KasI G'GCGC_C
Cuts at: 3606 4779 3606
Size: 1173 6496
KpnI G_GTAC'C
Cuts at: 4994 4994
Size: 7669
MfeI C'AATT_G
Cuts at: 5268 5268
Size: 7669
MluI A'CGCG_T
Cuts at: 7545 7545
Size: 7669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2791 4026 6783 6967 1305
Size: 1486 1235 2757 184 2007
Fragments arranged by size:
2757 2007 1486 1235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4311 10
Size: 54 1425 343 2479 3368
Fragments arranged by size:
3368 2479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2520 2652 2789 3561 3921 5627
Size: 648 976 378 132 137 772 360 1706
Cuts at: 5627 5986 6145 518
Size: 359 159 2042
Fragments arranged by size:
2042 1706 976 772 648 378 360 359
159 137 132
MspA1I CmG'CkG
Cuts at: 1606 2082 2652 2802 3054 3259 3429 3547 4269
Size: 476 570 150 252 205 170 118 722
Cuts at: 4269 4278 4701 5085 5762 6910 7155 1606
Size: 9 423 384 677 1148 245 2120
Fragments arranged by size:
2120 1148 722 677 570 476 423 384
252 245 205 170 150 118 9
NaeI GCC'GGC
Cuts at: 3776 3776
Size: 7669
NarI GG'CG_CC
Cuts at: 3607 4780 3607
Size: 1173 6496
NcoI C'CATG_G
Cuts at: 513 1065 2004 2409 2515 513
Size: 552 939 405 106 5667
Fragments arranged by size:
5667 939 552 405 106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7669
NgoMIV G'CCGG_C
Cuts at: 3774 3774
Size: 7669
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7669
NotI GC'GGCC_GC
Cuts at: 5116 5116
Size: 7669
NspI r_CATG'y
Cuts at: 1132 1229 2772 2844 3699 3861 5027 6572 1132
Size: 97 1543 72 855 162 1166 1545 2229
Fragments arranged by size:
2229 1545 1543 1166 855 162 97 72
PasI CC'CwG_GG
Cuts at: 1348 2050 2090 1348
Size: 702 40 6927
Fragments arranged by size:
6927 702 40
PciI A'CATG_T
Cuts at: 6568 6568
Size: 7669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3973 1108
Size: 2865 4804
PfoI T'CCnGG_A
Cuts at: 4206 4206
Size: 7669
PmeI GTTT'AAAC
Cuts at: 4973 4973
Size: 7669
PmlI CAC'GTG
Cuts at: 2679 2724 2679
Size: 45 7624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5738 5770 7251 7283 5738
Size: 32 1481 32 6124
Fragments arranged by size:
6124 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2364 1613
Size: 751 6918
PsiI TTA'TAA
Cuts at: 5239 5239
Size: 7669
PspOMI G'GGCC_C
Cuts at: 3252 3422 3252
Size: 170 7499
PstI C_TGCA'G
Cuts at: 830 2573 2826 3269 3393 3741 5021 830
Size: 1743 253 443 124 348 1280 3478
Fragments arranged by size:
3478 1743 1280 443 348 253 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7669
PvuII CAG'CTG
Cuts at: 2652 2802 3259 3429 2652
Size: 150 457 170 6892
Fragments arranged by size:
6892 457 170 150
SacI G_AGCT'C
Cuts at: 3933 3933
Size: 7669
SacII CC_GC'GG
Cuts at: 3055 3055
Size: 7669
SalI G'TCGA_C
Cuts at: 1936 2099 5011 1936
Size: 163 2912 4594
Fragments arranged by size:
4594 2912 163
SbfI CC_TGCA'GG
Cuts at: 3741 5021 3741
Size: 1280 6389
ScaI AGT'ACT
Cuts at: 5917 7629 5917
Size: 1712 5957
SfcI C'TryA_G
Cuts at: 826 1045 2296 2569 2822 3265 3389 3737 3878
Size: 219 1251 273 253 443 124 348 141
Cuts at: 3878 5017 6152 6833 7024 826
Size: 1139 1135 681 191 1471
Fragments arranged by size:
1471 1251 1139 1135 681 443 348 273
253 219 191 141 124
SfoI GGC'GCC
Cuts at: 3608 4781 3608
Size: 1173 6496
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7669
SmaI CCC'GGG
Cuts at: 1872 2289 3027 3174 3251 4141 4996 1872
Size: 417 738 147 77 890 855 4545
Fragments arranged by size:
4545 890 855 738 417 147 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3581 5773 6674 6936 820
Size: 197 926 1638 2192 901 262 1553
Fragments arranged by size:
2192 1638 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5460 493
Size: 4967 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7669
SphI G_CATG'C
Cuts at: 1132 5027 1132
Size: 3895 3774
SrfI GCCC'GGGC
Cuts at: 4141 4141
Size: 7669
SspI AAT'ATT
Cuts at: 5 52 5593 5
Size: 47 5541 2081
Fragments arranged by size:
5541 2081 47
StuI AGG'CCT
Cuts at: 1517 3686 4109 1517
Size: 2169 423 5077
Fragments arranged by size:
5077 2169 423
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2515 3144 3630 3834 4896
Size: 552 939 405 106 629 486 204 1062
Cuts at: 4896 5108 513
Size: 212 3074
Fragments arranged by size:
3074 1062 939 629 552 486 405 212
204 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 4277 5696 5713 5866 6051 6496
Size: 1255 2349 348 1419 17 153 185 445
Cuts at: 6496 325
Size: 1498
Fragments arranged by size:
2349 1498 1419 1255 445 348 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4878 5915 7627
Size: 274 80 33 51 1443 2901 1037 1712
Cuts at: 7627 96
Size: 138
Fragments arranged by size:
2901 1712 1443 1037 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7629 7661 7629
Size: 32 7637
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7669
XbaI T'CTAG_A
Cuts at: 5005 5005
Size: 7669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 4304 2554
Size: 1750 5919
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7669
XmaI C'CCGG_G
Cuts at: 1870 2287 3025 3172 3249 4139 4994 1870
Size: 417 738 147 77 890 855 4545
Fragments arranged by size:
4545 890 855 738 417 147 77
XmnI GAAnn'nnTTC
Cuts at: 4871 5798 4871
Size: 927 6742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5477 276
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SwaI