Restriction map for
pFN21AE1062
(Circular) (Six-base) MAPSORT of: pFN21AE1062.seq Check: 2514 from: 1 to: 9067
pFN21AE1062 9067 bp
With 182 enzymes: SgfI *
March 5, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2897 3457 4042 2897
Size: 560 585 7922
Fragments arranged by size:
7922 585 560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6877 278
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5657 6410 1937
Size: 3720 753 4594
Fragments arranged by size:
4594 3720 753
Acc65I G'GTAC_C
Cuts at: 6388 6388
Size: 9067
AclI AA'CG_TT
Cuts at: 1563 2150 7194 7567 1563
Size: 587 5044 373 3063
Fragments arranged by size:
5044 3063 587 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2661 2805 3056 3366 4302 5169 5985 6264
Size: 1029 144 251 310 936 867 816 279
Cuts at: 6264 7130 8514 8782 1632
Size: 866 1384 268 1917
Fragments arranged by size:
1917 1384 1029 936 867 866 816 310
279 268 251 144
AfeI AGC'GCT
Cuts at: 4439 4439
Size: 9067
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8870
AflIII A'CryG_T
Cuts at: 1203 3520 7966 8943 1203
Size: 2317 4446 977 1327
Fragments arranged by size:
4446 2317 1327 977
AgeI A'CCGG_T
Cuts at: 7898 7898
Size: 9067
AhdI GACnn_n'nnGTC
Cuts at: 4234 4818 5653 7796 4234
Size: 584 835 2143 5505
Fragments arranged by size:
5505 2143 835 584
AleI CACnn'nnGTG
Cuts at: 1166 5333 1166
Size: 4167 4900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 4050 1184
Size: 1179 1687 6201
Fragments arranged by size:
6201 1687 1179
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2140 2172 2140
Size: 32 9035
AlwNI CAG_nnn'CTG
Cuts at: 2732 3755 3982 5186 6810 8382 2732
Size: 1023 227 1204 1624 1572 3417
Fragments arranged by size:
3417 1624 1572 1204 1023 227
ApaLI G'TGCA_C
Cuts at: 7124 8280 7124
Size: 1156 7911
ApoI r'AATT_y
Cuts at: 1493 3029 3768 4000 4796 5443 5620 6376 6602
Size: 1536 739 232 796 647 177 756 226
Cuts at: 6602 6754 8863 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1536 796 756 739 647 232
226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 3852 7621 160
Size: 872 2820 3769 1606
Fragments arranged by size:
3769 2820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 3390 5824 6392 1870
Size: 73 132 1315 2434 568 4545
Fragments arranged by size:
4545 2434 1315 568 132 73
AvrII C'CTAG_G
Cuts at: 5215 5215
Size: 9067
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5063 5096 5063
Size: 33 9034
BamHI G'GATC_C
Cuts at: 4462 5219 5475 6397 6764 4462
Size: 757 256 922 367 6765
Fragments arranged by size:
6765 922 757 367 256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 2086 2093 6388 7843
Size: 325 217 627 292 7 7 4295 1455
Cuts at: 7843 618
Size: 1842
Fragments arranged by size:
4295 1842 1455 627 325 292 217 7
7
BanII G_rGCy'C
Cuts at: 1391 2026 3274 5270 6386 1391
Size: 635 1248 1996 1116 4072
Fragments arranged by size:
4072 1996 1248 1116 635
BbeI G_GCGC'C
Cuts at: 2083 2090 2097 2083
Size: 7 7 9053
Fragments arranged by size:
9053 7 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2447 6017 6182 6279 928
Size: 971 548 3570 165 97 3716
Fragments arranged by size:
3716 3570 971 548 165 97
BbvCI CC'TCA_GC
Cuts at: 2036 2036
Size: 9067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4629 4663 5388 5422 7258 7292 1903
Size: 34 2692 34 725 34 1836 34 3678
Fragments arranged by size:
3678 2692 1836 725 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2179 6959 8169 2179
Size: 4780 1210 3077
Fragments arranged by size:
4780 3077 1210
BclI T'GATC_A
Cuts at: 1548 5950 1548
Size: 4402 4665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 7678 136
Size: 107 122 71 631 605 337 5669 1525
Fragments arranged by size:
5669 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5467 9062 5467
Size: 3595 5472
BlpI GC'TnA_GC
Cuts at: 6484 6484
Size: 9067
Bme1580I G_kGCm'C
Cuts at: 1790 5740 6241 7128 8284 1790
Size: 3950 501 887 1156 2573
Fragments arranged by size:
3950 2573 1156 887 501
BmgBI CAC'GTC
Cuts at: 1318 3438 1318
Size: 2120 6947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3957 5038 6228
Size: 416 53 83 399 24 2540 1081 1190
Cuts at: 6228 6798 7756 442
Size: 570 958 1753
Fragments arranged by size:
2540 1753 1190 1081 958 570 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3657 4356 6228 3657
Size: 699 1872 6496
Fragments arranged by size:
6496 1872 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2939 3288 3936 4023 4329 5208 5448 5502
Size: 1700 349 648 87 306 879 240 54
Cuts at: 5502 5513 5767 5784 6075 7727 1239
Size: 11 254 17 291 1652 2579
Fragments arranged by size:
2579 1700 1652 879 648 349 306 291
254 240 87 54 17 11
Bpu10I CC'TnA_GC
Cuts at: 2036 3625 2036
Size: 1589 7478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2982 5550 7192 8057 8355 2982
Size: 2568 1642 865 298 3694
Fragments arranged by size:
3694 2568 1642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3418 4109 5677 6248 7730 882
Size: 617 1919 691 1568 571 1482 2219
Fragments arranged by size:
2219 1919 1568 1482 691 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 3738 4303 4635 6858 493
Size: 711 2534 565 332 2223 2702
Fragments arranged by size:
2702 2534 2223 711 565 332
BsaBI GATnn'nnATC
Cuts at: 9061 9061
Size: 9067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 2087 2094 2305
Size: 53 83 186 936 547 7 7 211
Cuts at: 2305 5322 6874 7256 7891 275
Size: 3017 1552 382 635 1451
Fragments arranged by size:
3017 1552 1451 936 635 547 382 211
186 83 53 7 7
BsaWI w'CCGG_w
Cuts at: 7500 7898 8172 8319 8730 7500
Size: 398 274 147 411 7837
Fragments arranged by size:
7837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2140 2170 3301 3331 5530
Size: 30 165 30 737 30 1131 30 2199
Cuts at: 5530 5560 1178
Size: 30 4685
Fragments arranged by size:
4685 2199 1131 737 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2876 3378 3963 4332 5268 5971 1186
Size: 838 852 502 585 369 936 703 4282
Fragments arranged by size:
4282 936 852 838 703 585 502 369
BseYI C'CCAG_C
Cuts at: 2498 2948 3685 4466 4714 5712 5721 5903 6141
Size: 450 737 781 248 998 9 182 238
Cuts at: 6141 8270 8766 2498
Size: 2129 496 2799
Fragments arranged by size:
2799 2129 998 781 737 496 450 248
238 182 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5929 6269 1163
Size: 573 4193 340 3961
Fragments arranged by size:
4193 3961 573 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 3585 6517 7278 8306 1540
Size: 275 186 102 1482 2932 761 1028 2301
Fragments arranged by size:
2932 2301 1482 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 7128 7213 8284 7128
Size: 85 1071 7911
Fragments arranged by size:
7911 1071 85
BsiWI C'GTAC_G
Cuts at: 4303 4303
Size: 9067
BsmI GAATG_Cn'
Cuts at: 2142 2350 2622 4741 5017 6578 6671 2142
Size: 208 272 2119 276 1561 93 4538
Fragments arranged by size:
4538 2119 1561 276 272 208 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5937 5937
Size: 9067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 3274 5270 5740 6241 6386 7128
Size: 399 236 1248 1996 470 501 145 742
Cuts at: 7128 7213 8284 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1996 1248 1071 742 501 470 399
236 145 85
BspEI T'CCGG_A
Cuts at: 8730 8730
Size: 9067
BspHI T'CATG_A
Cuts at: 4615 5482 5535 6956 8686 4615
Size: 867 53 1421 1730 4996
Fragments arranged by size:
4996 1730 1421 867 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2441 2897 3457 4042 6422 844
Size: 1597 456 560 585 2380 3489
Fragments arranged by size:
3489 2380 1597 585 560 456
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 2112 4312 5244 6954 1394
Size: 429 194 95 2200 932 1710 3507
Fragments arranged by size:
3507 2200 1710 932 429 194 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 2347 2638 3234 3766 6116 7562 7736
Size: 1183 1099 291 596 532 2350 1446 174
Cuts at: 7736 65
Size: 1396
Fragments arranged by size:
2350 1446 1396 1183 1099 596 532 291
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 5202 6207 7711 7898 1700
Size: 110 369 3023 1005 1504 187 2869
Fragments arranged by size:
3023 2869 1504 1005 369 187 110
BsrGI T'GTAC_A
Cuts at: 96 3588 96
Size: 3492 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9067
BssSI C'ACGA_G
Cuts at: 4272 7127 8139 4272
Size: 2855 1012 5200
Fragments arranged by size:
5200 2855 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9067
BstBI TT'CG_AA
Cuts at: 2146 3943 5893 7871 2146
Size: 1797 1950 1978 3342
Fragments arranged by size:
3342 1978 1950 1797
BstEII G'GTnAC_C
Cuts at: 1184 4810 1184
Size: 3626 5441
BstXI CCAn_nnnn'nTGG
Cuts at: 4423 5338 5472 6316 4423
Size: 915 134 844 7174
Fragments arranged by size:
7174 915 844 134
BstYI r'GATC_y
Cuts at: 1972 2324 2728 3457 4462 4564 5219 5467 5475
Size: 352 404 729 1005 102 655 248 8
Cuts at: 5475 6397 6764 7149 7166 8618 8704 8716 9062
Size: 922 367 385 17 1452 86 12 346
Cuts at: 9062 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 922 729 655 404 385
367 352 346 248 102 86 17 12
8
Bsu36I CC'TnA_GG
Cuts at: 2733 3666 5179 2733
Size: 933 1513 6621
Fragments arranged by size:
6621 1513 933
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 4852 5592 5834 513
Size: 552 939 93 2755 740 242 3746
Fragments arranged by size:
3746 2755 939 740 552 242 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8328
Fragments arranged by size:
8328 683 56
BtsI GCAGTG_nn'
Cuts at: 3374 3644 6030 6583 7377 7397 3374
Size: 270 2386 553 794 20 5044
Fragments arranged by size:
5044 2386 794 553 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9032
DraI TTT'AAA
Cuts at: 2283 4776 6371 2283
Size: 2493 1595 4979
Fragments arranged by size:
4979 2493 1595
DraIII CAC_nnn'GTG
Cuts at: 4480 4558 6172 4480
Size: 78 1614 7375
Fragments arranged by size:
7375 1614 78
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 8074 809
Size: 507 2822 3936 1802
Fragments arranged by size:
3936 2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2394 5991
Size: 54 1425 50 275 18 270 294 3597
Cuts at: 5991 6514 7403 9032 8
Size: 523 889 1629 43
Fragments arranged by size:
3597 1629 1425 889 523 294 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 6514 1537
Size: 275 288 4414 4090
Fragments arranged by size:
4414 4090 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2212 4302 5932 6056 6997 1353
Size: 859 2090 1630 124 941 3423
Fragments arranged by size:
3423 2090 1630 941 859 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 3457 7654 8028 8174 387
Size: 1064 138 438 1430 4197 374 146 1280
Fragments arranged by size:
4197 1430 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2661 2805 2939 3056 3288 3366 3936
Size: 393 1029 144 134 117 232 78 570
Cuts at: 3936 4023 4302 4329 5169 5208 5448 5502 5513
Size: 87 279 27 840 39 240 54 11
Cuts at: 5513 5767 5784 5985 6075 6264 7130 7727 8514
Size: 254 17 201 90 189 866 597 787
Cuts at: 8514 8782 1239
Size: 268 1524
Fragments arranged by size:
1524 1029 866 840 787 597 570 393
279 268 254 240 232 201 189 144
134 117 90 87 78 54 39 27
17 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2373 2373
Size: 9067
EcoO109I rG'GnC_Cy
Cuts at: 5138 5959 6095 6343 5138
Size: 821 136 248 7862
Fragments arranged by size:
7862 821 248 136
EcoRI G'AATT_C
Cuts at: 3029 4796 5443 6376 6754 3029
Size: 1767 647 933 378 5342
Fragments arranged by size:
5342 1767 933 647 378
EcoRV GAT'ATC
Cuts at: 2742 5572 2742
Size: 2830 6237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3023 3858 3023
Size: 835 8232
FspI TGC'GCA
Cuts at: 7573 7573
Size: 9067
HaeII r_GCGC'y
Cuts at: 1678 2083 2090 2097 4441 8214 1678
Size: 405 7 7 2344 3773 2531
Fragments arranged by size:
3773 2531 2344 405 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2726 2758 4141
Size: 32 135 32 605 32 517 32 1383
Cuts at: 4141 4173 4220 4252 4454 4486 4534 4566 4804
Size: 32 47 32 202 32 48 32 238
Cuts at: 4804 4836 5204 5236 5530 5562 5639 5671 7708
Size: 32 368 32 294 32 77 32 2037
Cuts at: 7708 7740 7782 7814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2037 1383 605 517 368 294 238
202 135 77 48 47 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5082 5584 6411 6657 669
Size: 988 281 3144 502 827 246 3079
Fragments arranged by size:
3144 3079 988 827 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9067
HpaI GTT'AAC
Cuts at: 5082 6657 5082
Size: 1575 7492
KasI G'GCGC_C
Cuts at: 2079 2086 2093 2079
Size: 7 7 9053
Fragments arranged by size:
9053 7 7
KpnI G_GTAC'C
Cuts at: 6392 6392
Size: 9067
MfeI C'AATT_G
Cuts at: 2988 6666 2988
Size: 3678 5389
MluI A'CGCG_T
Cuts at: 8943 8943
Size: 9067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2861 3137 3232 4901 5247 6249 8181 8365
Size: 1556 276 95 1669 346 1002 1932 184
Cuts at: 8365 1305
Size: 2007
Fragments arranged by size:
2007 1932 1669 1556 1002 346 276 184
95
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 5993 10
Size: 54 1425 343 564 3597 3084
Fragments arranged by size:
3597 3084 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4328 4421 4574 4937 5333 5336 5852
Size: 648 3162 93 153 363 396 3 516
Cuts at: 5852 6263 7025 7384 7543 518
Size: 411 762 359 159 2042
Fragments arranged by size:
3162 2042 762 648 516 411 396 363
359 159 153 93 3
MspA1I CmG'CkG
Cuts at: 1606 2099 4138 4147 6483 7160 8308 8553 1606
Size: 493 2039 9 2336 677 1148 245 2120
Fragments arranged by size:
2336 2120 2039 1148 677 493 245 9
NaeI GCC'GGC
Cuts at: 5204 6209 5204
Size: 1005 8062
NarI GG'CG_CC
Cuts at: 2080 2087 2094 2080
Size: 7 7 9053
Fragments arranged by size:
9053 7 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 4852 5592 5834 513
Size: 552 939 2848 740 242 3746
Fragments arranged by size:
3746 2848 939 740 552 242
NdeI CA'TA_TG
Cuts at: 387 3706 387
Size: 3319 5748
NgoMIV G'CCGG_C
Cuts at: 5202 6207 5202
Size: 1005 8062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9067
NotI GC'GGCC_GC
Cuts at: 2100 6514 2100
Size: 4414 4653
NspI r_CATG'y
Cuts at: 1132 1229 5656 5943 6425 7970 1132
Size: 97 4427 287 482 1545 2229
Fragments arranged by size:
4427 2229 1545 482 287 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9067
PciI A'CATG_T
Cuts at: 7966 7966
Size: 9067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2847 3755 1108
Size: 1739 908 6420
Fragments arranged by size:
6420 1739 908
PfoI T'CCnGG_A
Cuts at: 2316 3913 6091 2316
Size: 1597 2178 5292
Fragments arranged by size:
5292 2178 1597
PmeI GTTT'AAAC
Cuts at: 6371 6371
Size: 9067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2172 7136 7168 8649 8681 2140
Size: 32 4964 32 1481 32 2526
Fragments arranged by size:
4964 2526 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5138 5959 6095 6343 5138
Size: 821 136 248 7862
Fragments arranged by size:
7862 821 248 136
PshAI GACnn'nnGTC
Cuts at: 1613 5143 1613
Size: 3530 5537
PsiI TTA'TAA
Cuts at: 3780 6637 3780
Size: 2857 6210
PstI C_TGCA'G
Cuts at: 830 6419 830
Size: 5589 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9067
PvuII CAG'CTG
Cuts at: 4138 4147 4138
Size: 9 9058
SacII CC_GC'GG
Cuts at: 2100 2100
Size: 9067
SalI G'TCGA_C
Cuts at: 1936 6409 1936
Size: 4473 4594
SanDI GG'GwC_CC
Cuts at: 5138 5138
Size: 9067
SapI GCTCTTCn'nnn_
Cuts at: 2212 6056 2212
Size: 3844 5223
SbfI CC_TGCA'GG
Cuts at: 6419 6419
Size: 9067
ScaI AGT'ACT
Cuts at: 7315 9027 7315
Size: 1712 7355
SfcI C'TryA_G
Cuts at: 826 1045 6415 7550 8231 8422 826
Size: 219 5370 1135 681 191 1471
Fragments arranged by size:
5370 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2081 2088 2095 2081
Size: 7 7 9053
Fragments arranged by size:
9053 7 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9067
SmaI CCC'GGG
Cuts at: 1872 5826 6394 1872
Size: 3954 568 4545
Fragments arranged by size:
4545 3954 568
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2997 5565 7171 8072 8334 820
Size: 197 926 1054 2568 1606 901 262 1553
Fragments arranged by size:
2568 1606 1553 1054 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6858 493
Size: 6365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9067
SphI G_CATG'C
Cuts at: 1132 6425 1132
Size: 5293 3774
SspI AAT'ATT
Cuts at: 5 52 6991 5
Size: 47 6939 2081
Fragments arranged by size:
6939 2081 47
StuI AGG'CCT
Cuts at: 1517 4091 4549 1517
Size: 2574 458 6035
Fragments arranged by size:
6035 2574 458
StyI C'CwwG_G
Cuts at: 513 1065 2004 3498 3987 4119 4852 4890 5215
Size: 552 939 1494 489 132 733 38 325
Cuts at: 5215 5592 5834 6506 513
Size: 377 242 672 3074
Fragments arranged by size:
3074 1494 939 733 672 552 489 377
325 242 132 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3555 4499 4903 6069 7094 7111 7264
Size: 1255 1975 944 404 1166 1025 17 153
Cuts at: 7264 7449 7894 325
Size: 185 445 1498
Fragments arranged by size:
1975 1498 1255 1166 1025 944 445 404
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3588 3786 4728
Size: 274 80 33 51 1443 1611 198 942
Cuts at: 4728 4832 5000 5433 7313 9025 96
Size: 104 168 433 1880 1712 138
Fragments arranged by size:
1880 1712 1611 1443 942 433 274 198
168 138 104 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6094 6126 9027 9059 6094
Size: 32 2901 32 6102
Fragments arranged by size:
6102 2901 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9067
XbaI T'CTAG_A
Cuts at: 6403 6403
Size: 9067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2256 2553 2844 3120 4456 5278 6265 2256
Size: 297 291 276 1336 822 987 5058
Fragments arranged by size:
5058 1336 987 822 297 291 276
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9067
XmaI C'CCGG_G
Cuts at: 1870 5824 6392 1870
Size: 3954 568 4545
Fragments arranged by size:
4545 3954 568
XmnI GAAnn'nnTTC
Cuts at: 4872 5889 6065 7196 4872
Size: 1017 176 1131 6743
Fragments arranged by size:
6743 1131 1017 176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6875 276
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BfrBI BstZ17I EcoICRI FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SexAI SfiI SrfI SwaI