Restriction map for
pFN21AE1055
(Circular) (Six-base) MAPSORT of: pFN21AE1055.seq Check: 5346 from: 1 to: 9445
pFN21AE1055 9445 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3540 4463 4986 3540
Size: 923 523 7999
Fragments arranged by size:
7999 923 523
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2942 4518 7255 278
Size: 53 83 186 936 1406 1576 2737 2468
Fragments arranged by size:
2737 2468 1576 1406 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6788 1937
Size: 4851 4594
Acc65I G'GTAC_C
Cuts at: 5967 6766 5967
Size: 799 8646
AclI AA'CG_TT
Cuts at: 1563 7572 7945 1563
Size: 6009 373 3063
Fragments arranged by size:
6009 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2557 2906 2973 3447 4883 5059 5126 5867
Size: 925 349 67 474 1436 176 67 741
Cuts at: 5867 6337 7508 8892 9160 1632
Size: 470 1171 1384 268 1917
Fragments arranged by size:
1917 1436 1384 1171 925 741 474 470
349 268 176 67 67
AfeI AGC'GCT
Cuts at: 6651 6651
Size: 9445
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9248
AflIII A'CryG_T
Cuts at: 1203 2290 3883 8344 9321 1203
Size: 1087 1593 4461 977 1327
Fragments arranged by size:
4461 1593 1327 1087 977
AgeI A'CCGG_T
Cuts at: 8276 8276
Size: 9445
AhdI GACnn_n'nnGTC
Cuts at: 8174 8174
Size: 9445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5153 5451 5876 6066 1184
Size: 3969 298 425 190 4563
Fragments arranged by size:
4563 3969 425 298 190
AlwNI CAG_nnn'CTG
Cuts at: 5091 7188 8760 5091
Size: 2097 1572 5776
Fragments arranged by size:
5776 2097 1572
ApaI G_GGCC'C
Cuts at: 4473 5382 6571 4473
Size: 909 1189 7347
Fragments arranged by size:
7347 1189 909
ApaLI G'TGCA_C
Cuts at: 3497 5802 7502 8658 3497
Size: 2305 1700 1156 4284
Fragments arranged by size:
4284 2305 1700 1156
ApoI r'AATT_y
Cuts at: 1493 2056 5305 5884 6725 6754 6980 7132 9241
Size: 563 3249 579 841 29 226 152 2109
Cuts at: 9241 1493
Size: 1697
Fragments arranged by size:
3249 2109 1697 841 579 563 226 152
29
AseI AT'TA_AT
Cuts at: 160 1032 2595 7999 160
Size: 872 1563 5404 1606
Fragments arranged by size:
5404 1606 1563 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9445
AvaI C'yCGr_G
Cuts at: 1870 1943 3348 3676 3961 4187 4486 4883 4895
Size: 73 1405 328 285 226 299 397 12
Cuts at: 4895 4930 5159 5399 5441 5456 5651 6007 6627
Size: 35 229 240 42 15 195 356 620
Cuts at: 6627 6770 1870
Size: 143 4545
Fragments arranged by size:
4545 1405 620 397 356 328 299 285
240 229 226 195 143 73 42 35
15 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5951 5984 5951
Size: 33 9412
BamHI G'GATC_C
Cuts at: 3189 6775 7142 3189
Size: 3586 367 5492
Fragments arranged by size:
5492 3586 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4971 5717 5813 5822 5967
Size: 325 217 627 3184 746 96 9 145
Cuts at: 5967 6183 6766 8221 618
Size: 216 583 1455 1842
Fragments arranged by size:
3184 1842 1455 746 627 583 325 217
216 145 96 9
BanII G_rGCy'C
Cuts at: 1391 2208 2304 2364 2675 3349 4021 4188 4473
Size: 817 96 60 311 674 672 167 285
Cuts at: 4473 4957 5382 6106 6571 6578 6764 1391
Size: 484 425 724 465 7 186 4072
Fragments arranged by size:
4072 817 724 674 672 484 465 425
311 285 186 167 96 60 7
BbeI G_GCGC'C
Cuts at: 4975 6187 4975
Size: 1212 8233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3333 3625 3896 4128 4871 928
Size: 971 1434 292 271 232 743 5502
Fragments arranged by size:
5502 1434 971 743 292 271 232
BbvCI CC'TCA_GC
Cuts at: 2773 4040 5084 2773
Size: 1267 1044 7134
Fragments arranged by size:
7134 1267 1044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 7636 7670 1903
Size: 34 2882 34 2783 34 3678
Fragments arranged by size:
3678 2882 2783 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2517 7337 8547 2517
Size: 4820 1210 3415
Fragments arranged by size:
4820 3415 1210
BclI T'GATC_A
Cuts at: 1548 2259 1548
Size: 711 8734
BfrBI ATG'CAT
Cuts at: 3082 3082
Size: 9445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5377 5421
Size: 107 122 71 631 605 337 3368 44
Cuts at: 5421 5460 8056 136
Size: 39 2596 1525
Fragments arranged by size:
3368 2596 1525 631 605 337 122 107
71 44 39
BglII A'GATC_T
Cuts at: 3707 9440 3707
Size: 5733 3712
BlpI GC'TnA_GC
Cuts at: 2207 2669 4760 6862 2207
Size: 462 2091 2102 4790
Fragments arranged by size:
4790 2102 2091 462
Bme1580I G_kGCm'C
Cuts at: 1790 3501 4473 5373 5382 5806 6571 7506 8662
Size: 1711 972 900 9 424 765 935 1156
Cuts at: 8662 1790
Size: 2573
Fragments arranged by size:
2573 1711 1156 972 935 900 765 424
9
BmgBI CAC'GTC
Cuts at: 1318 5115 5290 1318
Size: 3797 175 5473
Fragments arranged by size:
5473 3797 175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 3463 3586
Size: 416 53 83 399 24 591 1455 123
Cuts at: 3586 5948 7176 8134 442
Size: 2362 1228 958 1753
Fragments arranged by size:
2362 1753 1455 1228 958 591 416 399
123 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 9445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2827 3738 4058 4603 4615 4705 4902
Size: 856 732 911 320 545 12 90 197
Cuts at: 4902 5249 6535 8105 1239
Size: 347 1286 1570 2579
Fragments arranged by size:
2579 1570 1286 911 856 732 545 347
320 197 90 12
Bpu10I CC'TnA_GC
Cuts at: 2773 3095 3112 4040 4580 5084 6572 2773
Size: 322 17 928 540 504 1488 5646
Fragments arranged by size:
5646 1488 928 540 504 322 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3061 3550 3724 4775 5519 5638 7570 8435 8733
Size: 489 174 1051 744 119 1932 865 298
Cuts at: 8733 3061
Size: 3773
Fragments arranged by size:
3773 1932 1051 865 744 489 298 174
119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4215 4420 8108 882
Size: 617 2716 205 3688 2219
Fragments arranged by size:
3688 2716 2219 617 205
BsaAI yAC'GTr
Cuts at: 493 1204 2293 2505 4336 7236 493
Size: 711 1089 212 1831 2900 2702
Fragments arranged by size:
2900 2702 1831 1089 711 212
BsaBI GATnn'nnATC
Cuts at: 9439 9439
Size: 9445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2939 4515 4640 4707
Size: 53 83 186 936 1406 1576 125 67
Cuts at: 4707 4832 4972 6184 6396 6632 7252 7634 8269
Size: 125 140 1212 212 236 620 382 635
Cuts at: 8269 275
Size: 1451
Fragments arranged by size:
1576 1451 1406 1212 936 635 620 382
236 212 186 140 125 125 83 67
53
BsaWI w'CCGG_w
Cuts at: 2509 2541 3876 5504 7878 8276 8550 8697 9108
Size: 32 1335 1628 2374 398 274 147 411
Cuts at: 9108 2509
Size: 2846
Fragments arranged by size:
2846 2374 1628 1335 411 398 274 147
32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 3530 3560 3854
Size: 30 165 30 597 30 1500 30 294
Cuts at: 3854 3884 4682 4712 5507 5537 1178
Size: 30 798 30 795 30 5086
Fragments arranged by size:
5086 1500 798 795 597 294 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2367 4410 4585 4948 5026 5515 5635 5975
Size: 1181 2043 175 363 78 489 120 340
Cuts at: 5975 6598 1186
Size: 623 4033
Fragments arranged by size:
4033 2043 1181 623 489 363 340 175
120 78
BseYI C'CCAG_C
Cuts at: 2704 3335 4751 4776 5204 5342 5633 6646 8648
Size: 631 1416 25 428 138 291 1013 2002
Cuts at: 8648 9144 2704
Size: 496 3005
Fragments arranged by size:
3005 2002 1416 1013 631 496 428 291
138 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3568 3829 4960 5315 1163
Size: 573 1832 261 1131 355 5293
Fragments arranged by size:
5293 1832 1131 573 355 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3953 4007 4661 6895 7656 8684
Size: 275 186 1952 54 654 2234 761 1028
Cuts at: 8684 1540
Size: 2301
Fragments arranged by size:
2301 2234 1952 1028 761 654 275 186
54
BsiHKAI G_wGCw'C
Cuts at: 2075 3501 3744 4335 5688 5806 5855 6305 6665
Size: 1426 243 591 1353 118 49 450 360
Cuts at: 6665 7506 7591 8662 2075
Size: 841 85 1071 2858
Fragments arranged by size:
2858 1426 1353 1071 841 591 450 360
243 118 85 49
BsmI GAATG_Cn'
Cuts at: 3805 4073 6956 7049 3805
Size: 268 2883 93 6201
Fragments arranged by size:
6201 2883 268 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2946 3073 2946
Size: 127 9318
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2208 2304 2364 2675 3349 3501
Size: 399 285 133 96 60 311 674 152
Cuts at: 3501 3744 4021 4188 4335 4473 4957 5373 5382
Size: 243 277 167 147 138 484 416 9
Cuts at: 5382 5688 5806 5855 6106 6305 6571 6578 6665
Size: 306 118 49 251 199 266 7 87
Cuts at: 6665 6764 7506 7591 8662 1391
Size: 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 484 416 399 311
306 285 277 266 251 243 199 167
152 147 138 133 118 99 96 87
85 60 49 9 7
BspEI T'CCGG_A
Cuts at: 2509 3876 5504 9108 2509
Size: 1367 1628 3604 2846
Fragments arranged by size:
3604 2846 1628 1367
BspHI T'CATG_A
Cuts at: 2428 7334 9064 2428
Size: 4906 1730 2809
Fragments arranged by size:
4906 2809 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2523 3540 4463 4986 5484 6257 6800 844
Size: 1679 1017 923 523 498 773 543 3489
Fragments arranged by size:
3489 1679 1017 923 773 543 523 498
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 4593 4629 4719 5625 5871 7332
Size: 429 796 1974 36 90 906 246 1461
Cuts at: 7332 1394
Size: 3507
Fragments arranged by size:
3507 1974 1461 906 796 429 246 90
36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2468 7940 8114 65
Size: 1183 1220 5472 174 1396
Fragments arranged by size:
5472 1396 1220 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2786 4656 5896 8089 8276 1700
Size: 110 976 1870 1240 2193 187 2869
Fragments arranged by size:
2869 2193 1870 1240 976 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9445
BssSI C'ACGA_G
Cuts at: 3256 5061 5135 7505 8517 3256
Size: 1805 74 2370 1012 4184
Fragments arranged by size:
4184 2370 1805 1012 74
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3496 5692 794
Size: 2702 2196 4547
Fragments arranged by size:
4547 2702 2196
BstBI TT'CG_AA
Cuts at: 8249 8249
Size: 9445
BstEII G'GTnAC_C
Cuts at: 1184 3284 1184
Size: 2100 7345
BstXI CCAn_nnnn'nTGG
Cuts at: 2806 2953 3524 4327 6673 2806
Size: 147 571 803 2346 5578
Fragments arranged by size:
5578 2346 803 571 147
BstYI r'GATC_y
Cuts at: 1972 2271 2529 3090 3189 3707 4602 6775 7142
Size: 299 258 561 99 518 895 2173 367
Cuts at: 7142 7527 7544 8996 9082 9094 9440 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2173 1977 1452 895 561 518 385 367
346 299 258 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4597 4597
Size: 9445
BtgI C'CryG_G
Cuts at: 513 1065 2004 2275 2317 2334 2662 4957 5002
Size: 552 939 271 42 17 328 2295 45
Cuts at: 5002 5118 5241 5374 5546 6345 513
Size: 116 123 133 172 799 3613
Fragments arranged by size:
3613 2295 939 799 552 328 271 172
133 123 116 45 42 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2622 5120 487
Size: 683 56 1396 2498 4812
Fragments arranged by size:
4812 2498 1396 683 56
BtsI GCAGTG_nn'
Cuts at: 3276 4269 6961 7755 7775 3276
Size: 993 2692 794 20 4946
Fragments arranged by size:
4946 2692 993 794 20
ClaI AT'CG_AT
Cuts at: 1554 4236 1554
Size: 2682 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4267 4302 6456 6491 529
Size: 35 3703 35 2154 35 3483
Fragments arranged by size:
3703 3483 2154 35 35 35
DraI TTT'AAA
Cuts at: 5304 6749 5304
Size: 1445 8000
DraIII CAC_nnn'GTG
Cuts at: 5008 5008
Size: 9445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8452 809
Size: 507 7136 1802
Fragments arranged by size:
7136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3950 4000
Size: 54 1425 50 275 18 910 1210 50
Cuts at: 4000 4658 4677 5109 5167 5422 6892 7781 9410
Size: 658 19 432 58 255 1470 889 1629
Cuts at: 9410 8
Size: 43
Fragments arranged by size:
1629 1470 1425 1210 910 889 658 432
275 255 58 54 50 50 43 19
18
EagI C'GGCC_G
Cuts at: 1537 1812 3950 4658 6892 1537
Size: 275 2138 708 2234 4090
Fragments arranged by size:
4090 2234 2138 708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2992 3061 5843 7375 1353
Size: 1639 69 2782 1532 3423
Fragments arranged by size:
3423 2782 1639 1532 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2312 2473 8032 8406 8552 387
Size: 1064 138 723 161 5559 374 146 1280
Fragments arranged by size:
5559 1280 1064 723 374 161 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2557 2827 2906 2973 3447 3738
Size: 393 463 462 270 79 67 474 291
Cuts at: 3738 4058 4603 4615 4705 4883 4902 5059 5126
Size: 320 545 12 90 178 19 157 67
Cuts at: 5126 5249 5867 6337 6535 7508 8105 8892 9160
Size: 123 618 470 198 973 597 787 268
Cuts at: 9160 1239
Size: 1524
Fragments arranged by size:
1524 973 787 618 597 545 474 470
463 462 393 320 291 270 268 198
178 157 123 90 79 67 67 19
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2524 3183 3386 4425 6410 2524
Size: 659 203 1039 1985 5559
Fragments arranged by size:
5559 1985 1039 659 203
EcoO109I rG'GnC_Cy
Cuts at: 2250 2280 4036 5047 5755 5950 6567 6568 2250
Size: 30 1756 1011 708 195 617 1 5127
Fragments arranged by size:
5127 1756 1011 708 617 195 30 1
EcoRI G'AATT_C
Cuts at: 2056 6754 7132 2056
Size: 4698 378 4369
Fragments arranged by size:
4698 4369 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3509 3509
Size: 9445
FseI GG_CCGG'CC
Cuts at: 4660 4660
Size: 9445
FspI TGC'GCA
Cuts at: 7951 7951
Size: 9445
HaeII r_GCGC'y
Cuts at: 1678 4557 4880 4975 5349 5514 5906 6187 6426
Size: 2879 323 95 374 165 392 281 239
Cuts at: 6426 6653 8592 1678
Size: 227 1939 2531
Fragments arranged by size:
2879 2531 1939 392 374 323 281 239
227 165 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 3854 3886 3924
Size: 32 135 32 428 32 1822 32 38
Cuts at: 3924 3956 4682 4714 5507 5539 8086 8118 8160
Size: 32 726 32 793 32 2547 32 42
Cuts at: 8160 8192 1373
Size: 32 2626
Fragments arranged by size:
2626 2547 1822 793 726 428 135 42
38 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6789 7035 669
Size: 988 281 4851 246 3079
Fragments arranged by size:
4851 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6130 748
Size: 5382 4063
HpaI GTT'AAC
Cuts at: 7035 7035
Size: 9445
KasI G'GCGC_C
Cuts at: 4971 6183 4971
Size: 1212 8233
KpnI G_GTAC'C
Cuts at: 5971 6770 5971
Size: 799 8646
MfeI C'AATT_G
Cuts at: 2412 7044 2412
Size: 4632 4813
MluI A'CGCG_T
Cuts at: 9321 9321
Size: 9445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3191 4200 4470 8559 8743 1305
Size: 1886 1009 270 4089 184 2007
Fragments arranged by size:
4089 2007 1886 1009 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2742 5111 5424 10
Size: 54 1425 343 910 2369 313 4031
Fragments arranged by size:
4031 2369 1425 910 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 3522 3810 3826 5858 6671 7403
Size: 648 1638 718 288 16 2032 813 732
Cuts at: 7403 7762 7921 518
Size: 359 159 2042
Fragments arranged by size:
2042 2032 1638 813 732 718 648 359
288 159 16
MspA1I CmG'CkG
Cuts at: 1606 2319 2552 2777 5091 5120 5548 5555 5881
Size: 713 233 225 2314 29 428 7 326
Cuts at: 5881 5918 6298 6402 6861 7538 8686 8931 1606
Size: 37 380 104 459 677 1148 245 2120
Fragments arranged by size:
2314 2120 1148 713 677 459 428 380
326 245 233 225 104 37 29 7
NaeI GCC'GGC
Cuts at: 2788 4658 2788
Size: 1870 7575
NarI GG'CG_CC
Cuts at: 4972 6184 4972
Size: 1212 8233
NcoI C'CATG_G
Cuts at: 513 1065 2004 2275 2334 5241 513
Size: 552 939 271 59 2907 4717
Fragments arranged by size:
4717 2907 939 552 271 59
NdeI CA'TA_TG
Cuts at: 387 2475 3612 3823 387
Size: 2088 1137 211 6009
Fragments arranged by size:
6009 2088 1137 211
NgoMIV G'CCGG_C
Cuts at: 2786 4656 2786
Size: 1870 7575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9445
NotI GC'GGCC_GC
Cuts at: 6892 6892
Size: 9445
NsiI A_TGCA'T
Cuts at: 3084 3084
Size: 9445
NspI r_CATG'y
Cuts at: 1132 1229 3082 3887 4534 5941 6001 6803 8348
Size: 97 1853 805 647 1407 60 802 1545
Cuts at: 8348 1132
Size: 2229
Fragments arranged by size:
2229 1853 1545 1407 805 802 647 97
60
PasI CC'CwG_GG
Cuts at: 1348 3341 1348
Size: 1993 7452
PciI A'CATG_T
Cuts at: 3883 8344 3883
Size: 4461 4984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4124 5487 6346 6499 1108
Size: 3016 1363 859 153 4054
Fragments arranged by size:
4054 3016 1363 859 153
PfoI T'CCnGG_A
Cuts at: 2140 5352 2140
Size: 3212 6233
PmeI GTTT'AAAC
Cuts at: 6749 6749
Size: 9445
PmlI CAC'GTG
Cuts at: 2293 2293
Size: 9445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3132 7514 7546 9027 9059 3100
Size: 32 4382 32 1481 32 3486
Fragments arranged by size:
4382 3486 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2280 4036 5950 2280
Size: 1756 1914 5775
Fragments arranged by size:
5775 1914 1756
PshAI GACnn'nnGTC
Cuts at: 1613 6240 1613
Size: 4627 4818
PsiI TTA'TAA
Cuts at: 7015 7015
Size: 9445
PspOMI G'GGCC_C
Cuts at: 4469 5378 6567 4469
Size: 909 1189 7347
Fragments arranged by size:
7347 1189 909
PspXI vC'TCGA_Gb
Cuts at: 4187 4895 5441 4187
Size: 708 546 8191
Fragments arranged by size:
8191 708 546
PstI C_TGCA'G
Cuts at: 830 3271 4460 4983 6219 6254 6315 6797 830
Size: 2441 1189 523 1236 35 61 482 3478
Fragments arranged by size:
3478 2441 1236 1189 523 482 61 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9445
PvuII CAG'CTG
Cuts at: 2777 5091 5881 5918 6402 2777
Size: 2314 790 37 484 5820
Fragments arranged by size:
5820 2314 790 484 37
SacII CC_GC'GG
Cuts at: 2320 5121 5549 2320
Size: 2801 428 6216
Fragments arranged by size:
6216 2801 428
SalI G'TCGA_C
Cuts at: 1936 6787 1936
Size: 4851 4594
SanDI GG'GwC_CC
Cuts at: 2280 2280
Size: 9445
SapI GCTCTTCn'nnn_
Cuts at: 2992 5843 2992
Size: 2851 6594
SbfI CC_TGCA'GG
Cuts at: 6254 6797 6254
Size: 543 8902
ScaI AGT'ACT
Cuts at: 7693 9405 7693
Size: 1712 7733
SfcI C'TryA_G
Cuts at: 826 1045 2676 3267 4456 4979 6215 6250 6311
Size: 219 1631 591 1189 523 1236 35 61
Cuts at: 6311 6410 6793 7928 8609 8800 826
Size: 99 383 1135 681 191 1471
Fragments arranged by size:
1631 1471 1236 1189 1135 681 591 523
383 219 191 99 61 35
SfoI GGC'GCC
Cuts at: 4973 6185 4973
Size: 1212 8233
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9445
SmaI CCC'GGG
Cuts at: 1872 4488 5401 6009 6772 1872
Size: 2616 913 608 763 4545
Fragments arranged by size:
4545 2616 913 763 608
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3040 3565 3676 3703 4187 4790
Size: 197 926 1097 525 111 27 484 603
Cuts at: 4790 4895 5441 5534 5617 7549 8450 8712 820
Size: 105 546 93 83 1932 901 262 1553
Fragments arranged by size:
1932 1553 1097 926 901 603 546 525
484 262 197 111 105 93 83 27
SnaBI TAC'GTA
Cuts at: 493 2505 7236 493
Size: 2012 4731 2702
Fragments arranged by size:
4731 2702 2012
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9445
SphI G_CATG'C
Cuts at: 1132 5941 6001 6803 1132
Size: 4809 60 802 3774
Fragments arranged by size:
4809 3774 802 60
SrfI GCCC'GGGC
Cuts at: 5401 5401
Size: 9445
SspI AAT'ATT
Cuts at: 5 52 7369 5
Size: 47 7317 2081
Fragments arranged by size:
7317 2081 47
StuI AGG'CCT
Cuts at: 1517 3178 3671 1517
Size: 1661 493 7291
Fragments arranged by size:
7291 1661 493
StyI C'CwwG_G
Cuts at: 513 1065 2004 2275 2334 2952 4021 5241 6076
Size: 552 939 271 59 618 1069 1220 835
Cuts at: 6076 6884 513
Size: 808 3074
Fragments arranged by size:
3074 1220 1069 939 835 808 618 552
271 59
SwaI ATTT'AAAT
Cuts at: 5304 5304
Size: 9445
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3497 4021 4217 4454 7472 7489 7642
Size: 1255 1917 524 196 237 3018 17 153
Cuts at: 7642 7827 8272 325
Size: 185 445 1498
Fragments arranged by size:
3018 1917 1498 1255 524 445 237 196
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7691 9403 96
Size: 274 80 33 51 1443 5714 1712 138
Fragments arranged by size:
5714 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 3530 3562 9405 9437 2181
Size: 32 1317 32 5843 32 2189
Fragments arranged by size:
5843 2189 1317 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9445
XbaI T'CTAG_A
Cuts at: 6781 6781
Size: 9445
XhoI C'TCGA_G
Cuts at: 1943 3676 4187 4895 5441 1943
Size: 1733 511 708 546 5947
Fragments arranged by size:
5947 1733 708 546 511
XmaI C'CCGG_G
Cuts at: 1870 4486 5399 6007 6770 1870
Size: 2616 913 608 763 4545
Fragments arranged by size:
4545 2616 913 763 608
XmnI GAAnn'nnTTC
Cuts at: 2814 3539 3581 3805 7574 2814
Size: 725 42 224 3769 4685
Fragments arranged by size:
4685 3769 725 224 42
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2940 4516 7253 276
Size: 53 83 186 936 1406 1576 2737 2468
Fragments arranged by size:
2737 2468 1576 1406 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I EcoICRI EcoRV FspAI
NruI PacI PsrI RsrII SacI SexAI SfiI XcmI