Restriction map for
pFN21AE1053
(Circular) (Six-base) MAPSORT of: pFN21AE1053.seq Check: 8046 from: 1 to: 5833
pFN21AE1053 5833 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 3036 2784
Size: 252 5581
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2731 2815 3896 5280 5548 1632
Size: 989 110 84 1081 1384 268 1917
Fragments arranged by size:
1917 1384 1081 989 268 110 84
AfeI AGC'GCT
Cuts at: 2660 2660
Size: 5833
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 2874 1166
Size: 1708 4125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 2462 2776 2866 3576 5148 2462
Size: 314 90 710 1572 3147
Fragments arranged by size:
3147 1572 710 314 90
ApaI G_GGCC'C
Cuts at: 2338 2469 2338
Size: 131 5702
ApaLI G'TGCA_C
Cuts at: 3119 3890 5046 3119
Size: 771 1156 3906
Fragments arranged by size:
3906 1156 771
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2480 3158 1870
Size: 73 525 12 678 4545
Fragments arranged by size:
4545 678 525 73 12
AvrII C'CTAG_G
Cuts at: 2014 2014
Size: 5833
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2426 3024 3065 3154 4609
Size: 325 217 627 639 598 41 89 1455
Cuts at: 4609 618
Size: 1842
Fragments arranged by size:
1842 1455 639 627 598 325 217 89
41
BanII G_rGCy'C
Cuts at: 1391 2238 2338 2469 2843 2927 3095 3152 1391
Size: 847 100 131 374 84 168 57 4072
Fragments arranged by size:
4072 847 374 168 131 100 84 57
BbeI G_GCGC'C
Cuts at: 2430 3069 2430
Size: 639 5194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BbvCI CC'TCA_GC
Cuts at: 2100 2100
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2471 2505 3064 3098 4024 4058 1903
Size: 34 534 34 559 34 926 34 3678
Fragments arranged by size:
3678 926 559 534 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 2736 2904
Size: 107 122 71 631 605 854 210 168
Cuts at: 2904 4444 136
Size: 1540 1525
Fragments arranged by size:
1540 1525 854 631 605 210 168 122
107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2338 2469 3123 3894 5050 1790
Size: 548 131 654 771 1156 2573
Fragments arranged by size:
2573 1156 771 654 548 131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2941 4493 1239
Size: 886 816 1552 2579
Fragments arranged by size:
2579 1552 886 816
Bpu10I CC'TnA_GC
Cuts at: 2100 2100
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2427 3066 3640 4022
Size: 53 83 186 936 894 639 574 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 936 894 639 635 574 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 1178
Size: 30 165 30 1560 30 4018
Fragments arranged by size:
4018 1560 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2332 1186
Size: 1146 4687
BseYI C'CCAG_C
Cuts at: 2085 2160 2415 2462 2497 3096 5036 5532 2085
Size: 75 255 47 35 599 1940 496 2386
Fragments arranged by size:
2386 1940 599 496 255 75 47 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2362 2468 2852 1163
Size: 573 626 106 384 4144
Fragments arranged by size:
4144 626 573 384 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2381 2515 2740 3007 3283 4044
Size: 275 186 380 134 225 267 276 761
Cuts at: 4044 5072 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 380 276 275 267 225
186 134
BsiHKAI G_wGCw'C
Cuts at: 2157 2843 2927 2934 2943 2981 3095 3123 3894
Size: 686 84 7 9 38 114 28 771
Cuts at: 3894 3979 5050 2157
Size: 85 1071 2940
Fragments arranged by size:
2940 1071 771 686 114 85 84 38
28 9 7
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2238 2338 2469 2843 2927 2934
Size: 399 367 81 100 131 374 84 7
Cuts at: 2934 2943 2981 3095 3123 3152 3894 3979 5050
Size: 9 38 114 28 29 742 85 1071
Cuts at: 5050 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 374 367 131 114
100 85 84 81 38 29 28 9
7
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2784 3036 3188 844
Size: 1940 252 152 3489
Fragments arranged by size:
3489 1940 252 152
BsrBI CCG'CTC
Cuts at: 1394 1823 2377 2485 3009 3720 1394
Size: 429 554 108 524 711 3507
Fragments arranged by size:
3507 711 554 524 429 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2348 2380 2520 4477 4664 1700
Size: 110 538 32 140 1957 187 2869
Fragments arranged by size:
2869 1957 538 187 140 110 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 2532 1925
Size: 607 5226
BssSI C'ACGA_G
Cuts at: 2093 2444 2645 3893 4905 2093
Size: 351 201 1248 1012 3021
Fragments arranged by size:
3021 1248 1012 351 201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2776 2901 794
Size: 1982 125 3726
Fragments arranged by size:
3726 1982 125
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2396 2396
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2176 3163 3530 3915 3932 5384 5470 5482
Size: 204 987 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 987 385 367 346 204 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2148 2315 2148
Size: 167 5666
BtgI C'CryG_G
Cuts at: 513 1065 2004 2113 2420 2665 3001 513
Size: 552 939 109 307 245 336 3345
Fragments arranged by size:
3345 939 552 336 307 245 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2378 2728 2737
Size: 54 1425 50 275 18 548 350 9
Cuts at: 2737 2821 3004 3085 3280 4169 5798 8
Size: 84 183 81 195 889 1629 43
Fragments arranged by size:
1629 1425 889 548 350 275 195 183
84 81 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2378 2737 3004 3280 1537
Size: 275 566 359 267 276 4090
Fragments arranged by size:
4090 566 359 276 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 2925 3093 2841
Size: 84 168 5581
Fragments arranged by size:
5581 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2621 2731 2815 2941 3896 4493
Size: 393 493 496 110 84 126 955 597
Cuts at: 4493 5280 5548 1239
Size: 787 268 1524
Fragments arranged by size:
1524 955 787 597 496 493 393 268
126 110 84
EcoO109I rG'GnC_Cy
Cuts at: 2275 2334 2275
Size: 59 5774
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 2306 2780 4339 2306
Size: 474 1559 3800
Fragments arranged by size:
3800 1559 474
HaeII r_GCGC'y
Cuts at: 1678 2303 2349 2430 2662 3069 4980 1678
Size: 625 46 81 232 407 1911 2531
Fragments arranged by size:
2531 1911 625 407 232 81 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373
Size: 32 135 32 2902 32 42 32 2626
Fragments arranged by size:
2902 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3177 3423 669
Size: 988 281 568 671 246 3079
Fragments arranged by size:
3079 988 671 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2426 3065 2426
Size: 639 5194
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 2495 2868 3006 4947 5131 1305
Size: 888 302 373 138 1941 184 2007
Fragments arranged by size:
2007 1941 888 373 302 184 138
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2124 2874 3791 4150 4309 518
Size: 648 946 12 750 917 359 159 2042
Fragments arranged by size:
2042 946 917 750 648 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2422 2459 2667 2863 3003 3115 3249 3926
Size: 816 37 208 196 140 112 134 677
Cuts at: 3926 5074 5319 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 245 208 196 140
134 112 37
NaeI GCC'GGC
Cuts at: 2350 2382 2522 2350
Size: 32 140 5661
Fragments arranged by size:
5661 140 32
NarI GG'CG_CC
Cuts at: 2427 3066 2427
Size: 639 5194
NcoI C'CATG_G
Cuts at: 513 1065 2004 2113 513
Size: 552 939 109 4233
Fragments arranged by size:
4233 939 552 109
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 2348 2380 2520 2348
Size: 32 140 5661
Fragments arranged by size:
5661 140 32
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 2737 3004 3280 2737
Size: 267 276 5290
Fragments arranged by size:
5290 276 267
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2334 2465 2334
Size: 131 5702
PstI C_TGCA'G
Cuts at: 830 2897 3185 830
Size: 2067 288 3478
Fragments arranged by size:
3478 2067 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SacI G_AGCT'C
Cuts at: 2843 2927 3095 2843
Size: 84 168 5581
Fragments arranged by size:
5581 168 84
SacII CC_GC'GG
Cuts at: 2423 2668 3004 2423
Size: 245 336 5252
Fragments arranged by size:
5252 336 245
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SexAI A'CCwGG_T
Cuts at: 2847 2847
Size: 5833
SfcI C'TryA_G
Cuts at: 826 1045 2893 3181 4316 4997 5188 826
Size: 219 1848 288 1135 681 191 1471
Fragments arranged by size:
1848 1471 1135 681 288 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2736 2736
Size: 5833
SfoI GGC'GCC
Cuts at: 2428 3067 2428
Size: 639 5194
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 2564 2828 2912 3080 1517
Size: 1047 264 84 168 4270
Fragments arranged by size:
4270 1047 264 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2014 2113 3272 513
Size: 552 939 10 99 1159 3074
Fragments arranged by size:
3074 1159 939 552 99 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 3860 3877 4030 4215 4660 325
Size: 1255 921 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3053 3962 3053
Size: 909 4924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SanDI SapI SrfI SwaI XcmI