Restriction map for
pFN21AE1051
(Circular) (Six-base) MAPSORT of: pFN21AE1051.seq Check: 374 from: 1 to: 6040
pFN21AE1051 6040 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3104 3104
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3383 1937
Size: 202 1244 4594
Fragments arranged by size:
4594 1244 202
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3317 4103 5487 5755 1632
Size: 1685 786 1384 268 1917
Fragments arranged by size:
1917 1685 1384 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 2834 1166
Size: 1668 4372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3112 1184
Size: 1928 4112
AlwNI CAG_nnn'CTG
Cuts at: 2154 2275 2797 3783 5355 2154
Size: 121 522 986 1572 2839
Fragments arranged by size:
2839 1572 986 522 121
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 1870
Size: 73 1422 4545
Fragments arranged by size:
4545 1422 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2166 2485 2716 3138
Size: 325 217 627 269 110 319 231 422
Cuts at: 3138 3361 4816 618
Size: 223 1455 1842
Fragments arranged by size:
1842 1455 627 422 325 319 269 231
223 217 110
BanII G_rGCy'C
Cuts at: 1391 2356 3111 3359 1391
Size: 965 755 248 4072
Fragments arranged by size:
4072 965 755 248
BbeI G_GCGC'C
Cuts at: 2720 2720
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2716 928
Size: 971 817 4252
Fragments arranged by size:
4252 971 817
BbvCI CC'TCA_GC
Cuts at: 2205 3242 2205
Size: 1037 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2190 2199 4651
Size: 107 122 71 631 605 518 9 2452
Cuts at: 4651 136
Size: 1525
Fragments arranged by size:
2452 1525 631 605 518 122 107 71
9
BglII A'GATC_T
Cuts at: 2242 6035 2242
Size: 3793 2247
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2161 2490 2497 3141 4101 5257 1790
Size: 269 102 329 7 644 960 1156 2573
Fragments arranged by size:
2573 1156 960 644 329 269 102 7
BmgBI CAC'GTC
Cuts at: 1318 2707 1318
Size: 1389 4651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2429 2484 3079
Size: 416 53 83 399 24 1012 55 595
Cuts at: 3079 3771 4729 442
Size: 692 958 1753
Fragments arranged by size:
1753 1012 958 692 595 416 399 83
55 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 2205 3242 2205
Size: 1037 5003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3831 493
Size: 711 1392 1235 2702
Fragments arranged by size:
2702 1392 1235 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2211 2717 2952
Size: 53 83 186 936 480 198 506 235
Cuts at: 2952 3324 3847 4229 4864 275
Size: 372 523 382 635 1451
Fragments arranged by size:
1451 936 635 523 506 480 382 372
235 198 186 83 53
BsaWI w'CCGG_w
Cuts at: 2296 4473 4871 5145 5292 5703 2296
Size: 2177 398 274 147 411 2633
Fragments arranged by size:
2633 2177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 2076 2154 2306 2481 5243 5739 2076
Size: 78 152 175 2762 496 2377
Fragments arranged by size:
2762 2377 496 175 152 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 4861
Fragments arranged by size:
4861 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3162 4101 4186 5257 3162
Size: 939 85 1071 3945
Fragments arranged by size:
3945 1071 939 85
BsmI GAATG_Cn'
Cuts at: 2332 3551 3644 2332
Size: 1219 93 4728
Fragments arranged by size:
4728 1219 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2161 2356 2490 2497 3111 3141
Size: 399 269 102 195 134 7 614 30
Cuts at: 3141 3162 3359 4101 4186 5257 1391
Size: 21 197 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 399 269 197 195
134 102 85 30 21 7
BspEI T'CCGG_A
Cuts at: 2296 5703 2296
Size: 3407 2633
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3104 3395 844
Size: 2260 291 3489
Fragments arranged by size:
3489 2260 291
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 2091 1925
Size: 166 5874
BssSI C'ACGA_G
Cuts at: 3053 4100 5112 3053
Size: 1047 1012 3981
Fragments arranged by size:
3981 1047 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 2497
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2085 2242 3370 3737 4122 4139 5591 5677
Size: 113 157 1128 367 385 17 1452 86
Cuts at: 5677 5689 6035 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 157 113
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 2197 2214 2436 513
Size: 552 939 26 167 17 222 4117
Fragments arranged by size:
4117 939 552 222 167 26 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2888 3556 4350 4370 2888
Size: 668 794 20 4558
Fragments arranged by size:
4558 794 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2389 2424 529
Size: 35 1825 35 4145
Fragments arranged by size:
4145 1825 35 35
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2433 2499
Size: 54 1425 50 275 18 361 242 66
Cuts at: 2499 2514 3049 3487 4376 6005 8
Size: 15 535 438 889 1629 43
Fragments arranged by size:
1629 1425 889 535 438 361 275 242
66 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2975 3173 3970 1353
Size: 1622 198 797 3423
Fragments arranged by size:
3423 1622 797 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2626 2645 2903 4627 5001 5147
Size: 1064 138 1037 19 258 1724 374 146
Cuts at: 5147 387
Size: 1280
Fragments arranged by size:
1724 1280 1064 1037 374 258 146 138
19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3317 4103 4700 5487 5755 1239
Size: 393 1685 786 597 787 268 1524
Fragments arranged by size:
1685 1524 787 786 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 3335 2360
Size: 975 5065
EcoO109I rG'GnC_Cy
Cuts at: 2039 2474 2989 3291 2039
Size: 435 515 302 4788
Fragments arranged by size:
4788 515 435 302
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 2258 2331 4546 2258
Size: 73 2215 3752
Fragments arranged by size:
3752 2215 73
HaeII r_GCGC'y
Cuts at: 1678 2134 2205 2720 5187 1678
Size: 456 71 515 2467 2531
Fragments arranged by size:
2531 2467 515 456 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4681 4713 4755 4787 1373
Size: 32 135 32 3109 32 42 32 2626
Fragments arranged by size:
3109 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 2716 2716
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2516 10
Size: 54 1425 343 603 81 3534
Fragments arranged by size:
3534 1425 603 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2834 2870 3023 3998 4357 4516
Size: 648 1275 393 36 153 975 359 159
Cuts at: 4516 518
Size: 2042
Fragments arranged by size:
2042 1275 975 648 393 359 159 153
36
MspA1I CmG'CkG
Cuts at: 1606 2032 2062 2154 2164 2335 2824 3085 3135
Size: 426 30 92 10 171 489 261 50
Cuts at: 3135 3211 3456 4133 5281 5526 1606
Size: 76 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 426 261 245 245
171 92 76 50 30 10
NarI GG'CG_CC
Cuts at: 2717 2717
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 2004 2436 513
Size: 552 939 432 4117
Fragments arranged by size:
4117 939 552 432
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2869 2881 3398 4943 1132
Size: 97 1640 12 517 1545 2229
Fragments arranged by size:
2229 1640 1545 517 97 12
PasI CC'CwG_GG
Cuts at: 1348 2171 2957 1348
Size: 823 786 4431
Fragments arranged by size:
4431 823 786
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 1108
Size: 1323 4717
PfoI T'CCnGG_A
Cuts at: 2991 2991
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PmlI CAC'GTG
Cuts at: 2596 2596
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2474 2989 3291 2039
Size: 435 515 302 4788
Fragments arranged by size:
4788 515 435 302
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2547 3392 830
Size: 1717 845 3478
Fragments arranged by size:
3478 1717 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2335 3085 2335
Size: 750 5290
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SanDI GG'GwC_CC
Cuts at: 2039 2039
Size: 6040
SapI GCTCTTCn'nnn_
Cuts at: 2975 2975
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2267 2543 3193 3388 4523 5204 5395
Size: 219 1222 276 650 195 1135 681 191
Cuts at: 5395 826
Size: 1471
Fragments arranged by size:
1471 1222 1135 681 650 276 219 195
191
SfoI GGC'GCC
Cuts at: 2718 2718
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 2881 3398 1132
Size: 1749 517 3774
Fragments arranged by size:
3774 1749 517
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 3039 3479 513
Size: 552 939 432 603 440 3074
Fragments arranged by size:
3074 939 603 552 440 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2184 2473 4067 4084 4237 4422 4867
Size: 1255 604 289 1594 17 153 185 445
Cuts at: 4867 325
Size: 1498
Fragments arranged by size:
1594 1498 1255 604 445 289 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 4286 5998
Size: 274 80 33 51 1443 822 1487 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
1712 1487 1443 822 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 3071 3284 1316
Size: 1755 213 4072
Fragments arranged by size:
4072 1755 213
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 3100 2443
Size: 657 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 3260 4169 3260
Size: 909 5131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI