Restriction map for
pFN21AE1048
(Circular) (Six-base) MAPSORT of: pFN21AE1048.seq Check: 9466 from: 1 to: 5896
pFN21AE1048 5896 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2661 3239 1937
Size: 724 578 4594
Fragments arranged by size:
4594 724 578
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2178 2358 2936 3959 5343 5611 1632
Size: 546 180 578 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1023 578 546 268 180
AflII C'TTAA_G
Cuts at: 820 1017 2936 820
Size: 197 1919 3780
Fragments arranged by size:
3780 1919 197
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 4882
AlwNI CAG_nnn'CTG
Cuts at: 3639 5211 3639
Size: 1572 4324
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2105 2319 3205 3431 3583 5692 1493
Size: 612 214 886 226 152 2109 1697
Fragments arranged by size:
2109 1697 886 612 226 214 152
AseI AT'TA_AT
Cuts at: 160 1032 2268 4450 160
Size: 872 1236 2182 1606
Fragments arranged by size:
2182 1606 1236 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 3217 4672 618
Size: 325 217 627 827 603 1455 1842
Fragments arranged by size:
1842 1455 827 627 603 325 217
BanII G_rGCy'C
Cuts at: 1391 3215 1391
Size: 1824 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 928
Size: 971 737 4188
Fragments arranged by size:
4188 971 737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BfrBI ATG'CAT
Cuts at: 2753 2753
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4507 136
Size: 107 122 71 631 605 2835 1525
Fragments arranged by size:
2835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 2040 3313 2040
Size: 1273 4623
Bme1580I G_kGCm'C
Cuts at: 1790 2914 3957 5113 1790
Size: 1124 1043 1156 2573
Fragments arranged by size:
2573 1156 1124 1043
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2974 3074 3627
Size: 416 53 83 399 24 1557 100 553
Cuts at: 3627 4585 442
Size: 958 1753
Fragments arranged by size:
1753 1557 958 553 416 399 100 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2394 2394
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2697 2796 4556 1239
Size: 1458 99 1760 2579
Fragments arranged by size:
2579 1760 1458 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4886 5184 4021
Size: 865 298 4733
Fragments arranged by size:
4733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 4559 882
Size: 617 744 2316 2219
Fragments arranged by size:
2316 2219 744 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 3076 5890 3076
Size: 2814 3082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3115 3703 4085 4720
Size: 53 83 186 936 1582 588 382 635
Cuts at: 4720 275
Size: 1451
Fragments arranged by size:
1582 1451 936 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4329 4727 5001 5148 5559 4329
Size: 398 274 147 411 4666
Fragments arranged by size:
4666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5671
Fragments arranged by size:
5671 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3013 3054 1186
Size: 1827 41 4028
Fragments arranged by size:
4028 1827 41
BseYI C'CCAG_C
Cuts at: 2254 5099 5595 2254
Size: 2845 496 2555
Fragments arranged by size:
2845 2555 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3075 1163
Size: 573 1339 3984
Fragments arranged by size:
3984 1339 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3191 3957 4042 5113 3191
Size: 766 85 1071 3974
Fragments arranged by size:
3974 1071 766 85
BsmI GAATG_Cn'
Cuts at: 2755 3407 3500 2755
Size: 652 93 5151
Fragments arranged by size:
5151 652 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2914 3191 3215 3957 4042 5113 1391
Size: 399 1124 277 24 742 85 1071 2174
Fragments arranged by size:
2174 1124 1071 742 399 277 85 24
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 3251 844
Size: 1803 604 3489
Fragments arranged by size:
3489 1803 604
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 2496 3045 4391 4565 65
Size: 1183 934 314 549 1346 174 1396
Fragments arranged by size:
1396 1346 1183 934 549 314 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2495 2750 794
Size: 1701 255 3940
Fragments arranged by size:
3940 1701 255
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 3056 1184
Size: 1872 4024
BstXI CCAn_nnnn'nTGG
Cuts at: 3052 3052
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 3007 3071 3226 3593 3978 3995 5447 5533
Size: 1035 64 155 367 385 17 1452 86
Cuts at: 5533 5545 5891 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 155 86
64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2484 513
Size: 552 939 480 3925
Fragments arranged by size:
3925 939 552 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 3412 4206 4226 2085
Size: 1327 794 20 3755
Fragments arranged by size:
3755 1327 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2823 2858 529
Size: 35 2259 35 3567
Fragments arranged by size:
3567 2259 35 35
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3160 3343 4232
Size: 54 1425 50 275 18 1330 183 889
Cuts at: 4232 5861 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1330 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3826 1353
Size: 2473 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2358 2697 2796 2936 3959 4556
Size: 393 546 180 339 99 140 1023 597
Cuts at: 4556 5343 5611 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1023 787 597 546 393 339 268
180 140 99
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
EcoRV GAT'ATC
Cuts at: 2428 2428
Size: 5896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 5896
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 2412 2444 2769
Size: 32 135 32 670 32 138 32 325
Cuts at: 2769 2801 2992 3024 4537 4569 4611 4643 1373
Size: 32 191 32 1513 32 42 32 2626
Fragments arranged by size:
2626 1513 670 325 191 138 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 3240 3486 669
Size: 988 281 220 1082 246 3079
Fragments arranged by size:
3079 1082 988 281 246 220
HindIII A'AGCT_T
Cuts at: 748 2414 2967 748
Size: 1666 553 3677
Fragments arranged by size:
3677 1666 553
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 2619 3495 2619
Size: 876 5020
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3118 5010 5194 1305
Size: 1813 1892 184 2007
Fragments arranged by size:
2007 1892 1813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3162 10
Size: 54 1425 343 1330 2744
Fragments arranged by size:
2744 1425 1330 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 3854 4213 4372 518
Size: 648 1323 1365 359 159 2042
Fragments arranged by size:
2042 1365 1323 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3312 3989 5137 5382 1606
Size: 1706 677 1148 245 2120
Fragments arranged by size:
2120 1706 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2484 513
Size: 552 939 480 3925
Fragments arranged by size:
3925 939 552 480
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NsiI A_TGCA'T
Cuts at: 2755 2755
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2222 2474 2672 3254 4799 1132
Size: 97 993 252 198 582 1545 2229
Fragments arranged by size:
2229 1545 993 582 252 198 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 1108
Size: 1878 4018
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 2187 2500 2559 2746 3248 830
Size: 1357 313 59 187 502 3478
Fragments arranged by size:
3478 1357 502 313 187 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 2984 4144 5856 2984
Size: 1160 1712 3024
Fragments arranged by size:
3024 1712 1160
SexAI A'CCwGG_T
Cuts at: 3096 3096
Size: 5896
SfcI C'TryA_G
Cuts at: 826 1045 2183 2496 2555 2742 3244 4379 5060
Size: 219 1138 313 59 187 502 1135 681
Cuts at: 5060 5251 826
Size: 191 1471
Fragments arranged by size:
1471 1138 1135 681 502 313 219 191
187 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2936 4000 4901 5163 820
Size: 197 926 993 1064 901 262 1553
Fragments arranged by size:
1553 1064 993 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 2672 3254 1132
Size: 1540 582 3774
Fragments arranged by size:
3774 1540 582
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 2070 1517
Size: 553 5343
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2484 3163 3335 513
Size: 552 939 111 369 679 172 3074
Fragments arranged by size:
3074 939 679 552 369 172 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3075 3923 3940 4093 4278 4723 325
Size: 1255 1495 848 17 153 185 445 1498
Fragments arranged by size:
1498 1495 1255 848 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2982 4142 5854
Size: 274 80 33 51 1443 1005 1160 1712
Cuts at: 5854 96
Size: 138
Fragments arranged by size:
1712 1443 1160 1005 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5856 5888 5856
Size: 32 5864
Tth111I GACn'n_nGTC
Cuts at: 1316 2931 1316
Size: 1615 4281
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 2081 4025 2081
Size: 1944 3952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI