(Circular) (Six-base) MAPSORT of: pFN21AE1043.seq Check: 3696 from: 1 to: 6115 pFN21AE1043 6115 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2111 3034 2111 Size: 923 5192 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 3458 1937 Size: 562 959 4594 Fragments arranged by size: 4594 959 562 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2431 2741 2986 4178 5562 5830 1632 Size: 799 310 245 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 799 310 268 245 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3066 1184 Size: 1882 4233 AlwNI CAG_nnn'CTG Cuts at: 2878 3858 5430 2878 Size: 980 1572 3563 Fragments arranged by size: 3563 1572 980 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3250 3424 3650 3802 5911 1493 Size: 1757 174 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 174 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2697 3440 1870 Size: 73 754 743 4545 Fragments arranged by size: 4545 754 743 73 BamHI G'GATC_C Cuts at: 3445 3812 3445 Size: 367 5748 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2415 2519 2784 3119 3436 Size: 325 217 627 628 104 265 335 317 Cuts at: 3436 4891 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 628 627 335 325 317 265 217 104 BanII G_rGCy'C Cuts at: 1391 3281 3434 1391 Size: 1890 153 4072 Fragments arranged by size: 4072 1890 153 BbeI G_GCGC'C Cuts at: 2419 2419 Size: 6115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2149 3364 928 Size: 971 250 1215 3679 Fragments arranged by size: 3679 1215 971 250 BbvCI CC'TCA_GC Cuts at: 3410 3410 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2870 2904 4306 4340 1903 Size: 34 50 34 849 34 1402 34 3678 Fragments arranged by size: 3678 1402 849 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4726 136 Size: 107 122 71 631 605 3054 1525 Fragments arranged by size: 3054 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2199 6110 2199 Size: 3911 2204 BlpI GC'TnA_GC Cuts at: 2366 3532 2366 Size: 1166 4949 Bme1580I G_kGCm'C Cuts at: 1790 2060 2168 2486 2524 2570 2648 2690 2789 Size: 270 108 318 38 46 78 42 99 Cuts at: 2789 2854 3086 4176 5332 1790 Size: 65 232 1090 1156 2573 Fragments arranged by size: 2573 1156 1090 318 270 232 108 99 78 65 46 42 38 BmgBI CAC'GTC Cuts at: 1318 3214 1318 Size: 1896 4219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2299 2921 4775 1239 Size: 812 248 622 1854 2579 Fragments arranged by size: 2579 1854 812 622 248 Bpu10I CC'TnA_GC Cuts at: 2790 3410 2790 Size: 620 5495 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 3014 3158 4240 5105 5403 2405 Size: 609 144 1082 865 298 3117 Fragments arranged by size: 3117 1082 865 609 298 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3215 4778 882 Size: 617 1716 1563 2219 Fragments arranged by size: 2219 1716 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 3270 3906 493 Size: 711 2066 636 2702 Fragments arranged by size: 2702 2066 711 636 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2416 3922 4304 4939 Size: 53 83 186 936 883 1506 382 635 Cuts at: 4939 275 Size: 1451 Fragments arranged by size: 1506 1451 936 883 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4548 4946 5220 5367 5778 4548 Size: 398 274 147 411 4885 Fragments arranged by size: 4885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 2831 2918 1186 Size: 1093 552 87 4383 Fragments arranged by size: 4383 1093 552 87 BseYI C'CCAG_C Cuts at: 2547 3007 5318 5814 2547 Size: 460 2311 496 2848 Fragments arranged by size: 2848 2311 496 460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 2370 2572 2581 1163 Size: 573 375 259 202 9 4697 Fragments arranged by size: 4697 573 375 259 202 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3565 4326 5354 1540 Size: 275 186 1564 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2751 2934 4176 4261 5332 2751 Size: 183 1242 85 1071 3534 Fragments arranged by size: 3534 1242 1071 183 85 BsmI GAATG_Cn' Cuts at: 3053 3626 3719 3053 Size: 573 93 5449 Fragments arranged by size: 5449 573 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2168 2486 2524 2570 2648 2690 Size: 399 270 108 318 38 46 78 42 Cuts at: 2690 2751 2789 2854 2934 3086 3281 3434 4176 Size: 61 38 65 80 152 195 153 742 Cuts at: 4176 4261 5332 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 318 270 195 153 152 108 85 80 78 65 61 46 42 38 38 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 2111 2135 2327 2392 2715 3034 3470 Size: 1239 28 24 192 65 323 319 436 Cuts at: 3470 844 Size: 3489 Fragments arranged by size: 3489 1239 436 323 319 192 65 28 24 BsrBI CCG'CTC Cuts at: 1394 1823 4002 1394 Size: 429 2179 3507 Fragments arranged by size: 3507 2179 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2805 3073 4610 4784 65 Size: 1183 1557 268 1537 174 1396 Fragments arranged by size: 1557 1537 1396 1183 268 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2862 4759 4946 1700 Size: 110 1052 1897 187 2869 Fragments arranged by size: 2869 1897 1052 187 110 BsrGI T'GTAC_A Cuts at: 96 3099 96 Size: 3003 3112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 4175 5187 4175 Size: 1012 5103 BstAPI GCAn_nnn'nTGC Cuts at: 794 2595 2878 794 Size: 1801 283 4031 Fragments arranged by size: 4031 1801 283 BstBI TT'CG_AA Cuts at: 3340 4919 3340 Size: 1579 4536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstXI CCAn_nnnn'nTGG Cuts at: 3270 3270 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2199 3405 3445 3812 4197 4214 5666 5752 Size: 227 1206 40 367 385 17 1452 86 Cuts at: 5752 5764 6110 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 227 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2169 2169 Size: 6115 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5563 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3079 3631 4425 4445 2926 Size: 153 552 794 20 4596 Fragments arranged by size: 4596 794 552 153 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2659 2694 3055 3090 529 Size: 35 2095 35 361 35 3554 Fragments arranged by size: 3554 2095 361 35 35 35 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2838 3562 Size: 54 1425 50 275 18 277 731 724 Cuts at: 3562 4451 6080 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 731 724 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4045 1353 Size: 2692 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4702 5076 5222 387 Size: 1064 138 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2299 2431 2741 2921 2986 4178 Size: 393 419 248 132 310 180 65 1192 Cuts at: 4178 4775 5562 5830 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1192 787 597 419 393 310 268 248 180 132 65 EcoO109I rG'GnC_Cy Cuts at: 2704 2704 Size: 6115 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 2419 5262 1678 Size: 741 2843 2531 Fragments arranged by size: 2843 2531 741 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2894 2926 4756 4788 4830 Size: 32 135 32 1322 32 1830 32 42 Cuts at: 4830 4862 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 1322 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KasI G'GCGC_C Cuts at: 2415 2415 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5229 5413 1305 Size: 3924 184 2007 Fragments arranged by size: 3924 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2840 10 Size: 54 1425 343 1008 3285 Fragments arranged by size: 3285 1425 1008 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3027 3107 4073 4432 4591 518 Size: 648 1861 80 966 359 159 2042 Fragments arranged by size: 2042 1861 966 648 359 159 80 MspA1I CmG'CkG Cuts at: 1606 2250 2277 2730 3531 4208 5356 5601 1606 Size: 644 27 453 801 677 1148 245 2120 Fragments arranged by size: 2120 1148 801 677 644 453 245 27 NaeI GCC'GGC Cuts at: 2864 2864 Size: 6115 NarI GG'CG_CC Cuts at: 2416 2416 Size: 6115 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5563 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 2862 2862 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 2555 2813 3473 5018 1132 Size: 97 1326 258 660 1545 2229 Fragments arranged by size: 2229 1545 1326 660 258 97 PasI CC'CwG_GG Cuts at: 1348 3191 1348 Size: 1843 4272 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PfoI T'CCnGG_A Cuts at: 2028 2028 Size: 6115 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PmlI CAC'GTG Cuts at: 3270 3270 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2704 2704 Size: 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PstI C_TGCA'G Cuts at: 830 2136 2324 2883 3045 3467 830 Size: 1306 188 559 162 422 3478 Fragments arranged by size: 3478 1306 559 422 188 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2730 2730 Size: 6115 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SbfI CC_TGCA'GG Cuts at: 2136 3467 2136 Size: 1331 4784 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2132 2300 2320 2879 2966 3041 3463 Size: 219 1087 168 20 559 87 75 422 Cuts at: 3463 4598 5279 5470 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 559 422 219 191 168 87 75 20 SfoI GGC'GCC Cuts at: 2417 2417 Size: 6115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 3442 1872 Size: 1570 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2993 3137 4219 5120 5382 Size: 197 926 477 573 144 1082 901 262 Cuts at: 5382 820 Size: 1553 Fragments arranged by size: 1553 1082 926 901 573 477 262 197 144 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 2555 3473 1132 Size: 1423 918 3774 Fragments arranged by size: 3774 1423 918 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 2459 1517 Size: 942 5173 StyI C'CwwG_G Cuts at: 513 1065 2648 2976 3554 513 Size: 552 1583 328 578 3074 Fragments arranged by size: 3074 1583 578 552 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4142 4159 4312 4497 4942 325 Size: 1255 2562 17 153 185 445 1498 Fragments arranged by size: 2562 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 3099 4361 Size: 274 80 33 51 1443 247 875 1262 Cuts at: 4361 6073 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1262 875 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 3440 1870 Size: 1570 4545 XmnI GAAnn'nnTTC Cuts at: 3251 4244 3251 Size: 993 5122 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI