Restriction map for
pFN21AE1043
(Circular) (Six-base) MAPSORT of: pFN21AE1043.seq Check: 3696 from: 1 to: 6115
pFN21AE1043 6115 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 3034 2111
Size: 923 5192
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 3458 1937
Size: 562 959 4594
Fragments arranged by size:
4594 959 562
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2431 2741 2986 4178 5562 5830 1632
Size: 799 310 245 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 799 310 268 245
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3066 1184
Size: 1882 4233
AlwNI CAG_nnn'CTG
Cuts at: 2878 3858 5430 2878
Size: 980 1572 3563
Fragments arranged by size:
3563 1572 980
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3250 3424 3650 3802 5911 1493
Size: 1757 174 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 174 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 3440 1870
Size: 73 754 743 4545
Fragments arranged by size:
4545 754 743 73
BamHI G'GATC_C
Cuts at: 3445 3812 3445
Size: 367 5748
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2415 2519 2784 3119 3436
Size: 325 217 627 628 104 265 335 317
Cuts at: 3436 4891 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 628 627 335 325 317 265
217 104
BanII G_rGCy'C
Cuts at: 1391 3281 3434 1391
Size: 1890 153 4072
Fragments arranged by size:
4072 1890 153
BbeI G_GCGC'C
Cuts at: 2419 2419
Size: 6115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 3364 928
Size: 971 250 1215 3679
Fragments arranged by size:
3679 1215 971 250
BbvCI CC'TCA_GC
Cuts at: 3410 3410
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2870 2904 4306 4340 1903
Size: 34 50 34 849 34 1402 34 3678
Fragments arranged by size:
3678 1402 849 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4726 136
Size: 107 122 71 631 605 3054 1525
Fragments arranged by size:
3054 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2199 6110 2199
Size: 3911 2204
BlpI GC'TnA_GC
Cuts at: 2366 3532 2366
Size: 1166 4949
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2168 2486 2524 2570 2648 2690 2789
Size: 270 108 318 38 46 78 42 99
Cuts at: 2789 2854 3086 4176 5332 1790
Size: 65 232 1090 1156 2573
Fragments arranged by size:
2573 1156 1090 318 270 232 108 99
78 65 46 42 38
BmgBI CAC'GTC
Cuts at: 1318 3214 1318
Size: 1896 4219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3846 4804 442
Size: 416 53 83 399 24 2429 958 1753
Fragments arranged by size:
2429 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2299 2921 4775 1239
Size: 812 248 622 1854 2579
Fragments arranged by size:
2579 1854 812 622 248
Bpu10I CC'TnA_GC
Cuts at: 2790 3410 2790
Size: 620 5495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 3014 3158 4240 5105 5403 2405
Size: 609 144 1082 865 298 3117
Fragments arranged by size:
3117 1082 865 609 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3215 4778 882
Size: 617 1716 1563 2219
Fragments arranged by size:
2219 1716 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 3906 493
Size: 711 2066 636 2702
Fragments arranged by size:
2702 2066 711 636
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2416 3922 4304 4939
Size: 53 83 186 936 883 1506 382 635
Cuts at: 4939 275
Size: 1451
Fragments arranged by size:
1506 1451 936 883 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4548 4946 5220 5367 5778 4548
Size: 398 274 147 411 4885
Fragments arranged by size:
4885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2831 2918 1186
Size: 1093 552 87 4383
Fragments arranged by size:
4383 1093 552 87
BseYI C'CCAG_C
Cuts at: 2547 3007 5318 5814 2547
Size: 460 2311 496 2848
Fragments arranged by size:
2848 2311 496 460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 2370 2572 2581 1163
Size: 573 375 259 202 9 4697
Fragments arranged by size:
4697 573 375 259 202 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3565 4326 5354 1540
Size: 275 186 1564 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 2934 4176 4261 5332 2751
Size: 183 1242 85 1071 3534
Fragments arranged by size:
3534 1242 1071 183 85
BsmI GAATG_Cn'
Cuts at: 3053 3626 3719 3053
Size: 573 93 5449
Fragments arranged by size:
5449 573 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2168 2486 2524 2570 2648 2690
Size: 399 270 108 318 38 46 78 42
Cuts at: 2690 2751 2789 2854 2934 3086 3281 3434 4176
Size: 61 38 65 80 152 195 153 742
Cuts at: 4176 4261 5332 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 318 270 195 153
152 108 85 80 78 65 61 46
42 38 38
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2111 2135 2327 2392 2715 3034 3470
Size: 1239 28 24 192 65 323 319 436
Cuts at: 3470 844
Size: 3489
Fragments arranged by size:
3489 1239 436 323 319 192 65 28
24
BsrBI CCG'CTC
Cuts at: 1394 1823 4002 1394
Size: 429 2179 3507
Fragments arranged by size:
3507 2179 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2805 3073 4610 4784 65
Size: 1183 1557 268 1537 174 1396
Fragments arranged by size:
1557 1537 1396 1183 268 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 4759 4946 1700
Size: 110 1052 1897 187 2869
Fragments arranged by size:
2869 1897 1052 187 110
BsrGI T'GTAC_A
Cuts at: 96 3099 96
Size: 3003 3112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 4175 5187 4175
Size: 1012 5103
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2595 2878 794
Size: 1801 283 4031
Fragments arranged by size:
4031 1801 283
BstBI TT'CG_AA
Cuts at: 3340 4919 3340
Size: 1579 4536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstXI CCAn_nnnn'nTGG
Cuts at: 3270 3270
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2199 3405 3445 3812 4197 4214 5666 5752
Size: 227 1206 40 367 385 17 1452 86
Cuts at: 5752 5764 6110 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 227 86
40 17 12
Bsu36I CC'TnA_GG
Cuts at: 2169 2169
Size: 6115
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 3079 3631 4425 4445 2926
Size: 153 552 794 20 4596
Fragments arranged by size:
4596 794 552 153 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2659 2694 3055 3090 529
Size: 35 2095 35 361 35 3554
Fragments arranged by size:
3554 2095 361 35 35 35
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2838 3562
Size: 54 1425 50 275 18 277 731 724
Cuts at: 3562 4451 6080 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 731 724 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4045 1353
Size: 2692 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4702 5076 5222 387
Size: 1064 138 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2299 2431 2741 2921 2986 4178
Size: 393 419 248 132 310 180 65 1192
Cuts at: 4178 4775 5562 5830 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1192 787 597 419 393 310 268
248 180 132 65
EcoO109I rG'GnC_Cy
Cuts at: 2704 2704
Size: 6115
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 2419 5262 1678
Size: 741 2843 2531
Fragments arranged by size:
2843 2531 741
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2894 2926 4756 4788 4830
Size: 32 135 32 1322 32 1830 32 42
Cuts at: 4830 4862 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 1322 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KasI G'GCGC_C
Cuts at: 2415 2415
Size: 6115
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5229 5413 1305
Size: 3924 184 2007
Fragments arranged by size:
3924 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 10
Size: 54 1425 343 1008 3285
Fragments arranged by size:
3285 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3027 3107 4073 4432 4591 518
Size: 648 1861 80 966 359 159 2042
Fragments arranged by size:
2042 1861 966 648 359 159 80
MspA1I CmG'CkG
Cuts at: 1606 2250 2277 2730 3531 4208 5356 5601 1606
Size: 644 27 453 801 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 644 453 245 27
NaeI GCC'GGC
Cuts at: 2864 2864
Size: 6115
NarI GG'CG_CC
Cuts at: 2416 2416
Size: 6115
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5563
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NgoMIV G'CCGG_C
Cuts at: 2862 2862
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 2555 2813 3473 5018 1132
Size: 97 1326 258 660 1545 2229
Fragments arranged by size:
2229 1545 1326 660 258 97
PasI CC'CwG_GG
Cuts at: 1348 3191 1348
Size: 1843 4272
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6115
PfoI T'CCnGG_A
Cuts at: 2028 2028
Size: 6115
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PmlI CAC'GTG
Cuts at: 3270 3270
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2704 2704
Size: 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2136 2324 2883 3045 3467 830
Size: 1306 188 559 162 422 3478
Fragments arranged by size:
3478 1306 559 422 188 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2730 2730
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 2136 3467 2136
Size: 1331 4784
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2132 2300 2320 2879 2966 3041 3463
Size: 219 1087 168 20 559 87 75 422
Cuts at: 3463 4598 5279 5470 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 559 422 219 191
168 87 75 20
SfoI GGC'GCC
Cuts at: 2417 2417
Size: 6115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 3442 1872
Size: 1570 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 2993 3137 4219 5120 5382
Size: 197 926 477 573 144 1082 901 262
Cuts at: 5382 820
Size: 1553
Fragments arranged by size:
1553 1082 926 901 573 477 262 197
144
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 2555 3473 1132
Size: 1423 918 3774
Fragments arranged by size:
3774 1423 918
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 1517
Size: 942 5173
StyI C'CwwG_G
Cuts at: 513 1065 2648 2976 3554 513
Size: 552 1583 328 578 3074
Fragments arranged by size:
3074 1583 578 552 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4142 4159 4312 4497 4942 325
Size: 1255 2562 17 153 185 445 1498
Fragments arranged by size:
2562 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 3099 4361
Size: 274 80 33 51 1443 247 875 1262
Cuts at: 4361 6073 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1262 875 274 247 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 3440 1870
Size: 1570 4545
XmnI GAAnn'nnTTC
Cuts at: 3251 4244 3251
Size: 993 5122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI