Restriction map for
pFN21AE1040
(Circular) (Six-base) MAPSORT of: pFN21AE1040.seq Check: 143 from: 1 to: 6049
pFN21AE1040 6049 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2460 2460
Size: 6049
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2345 3392 1937
Size: 408 1047 4594
Fragments arranged by size:
4594 1047 408
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2432 2849 2990 3243 4112 5496 5764
Size: 464 336 417 141 253 869 1384 268
Cuts at: 5764 1632
Size: 1917
Fragments arranged by size:
1917 1384 869 464 417 336 268 253
141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 2053 3132 4948 5925 1203
Size: 850 1079 1816 977 1327
Fragments arranged by size:
1816 1327 1079 977 850
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 3133 4778 3133
Size: 1645 4404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2859 3275 1184
Size: 1675 416 3958
Fragments arranged by size:
3958 1675 416
AlwNI CAG_nnn'CTG
Cuts at: 2341 2485 3792 5364 2341
Size: 144 1307 1572 3026
Fragments arranged by size:
3026 1572 1307 144
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3358 3584 3736 5845 1493
Size: 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 3374 1870
Size: 73 1065 366 4545
Fragments arranged by size:
4545 1065 366 73
BamHI G'GATC_C
Cuts at: 2289 2502 3379 3746 2289
Size: 213 877 367 4592
Fragments arranged by size:
4592 877 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3370 4825 618
Size: 325 217 627 1583 1455 1842
Fragments arranged by size:
1842 1583 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3368 1391
Size: 621 1356 4072
Fragments arranged by size:
4072 1356 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2038 2072 2600 2634 4240 4274 1903
Size: 34 101 34 528 34 1606 34 3678
Fragments arranged by size:
3678 1606 528 101 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2095 6044 2095
Size: 3949 2100
BlpI GC'TnA_GC
Cuts at: 2021 2045 2672 3466 2021
Size: 24 627 794 4604
Fragments arranged by size:
4604 794 627 24
Bme1580I G_kGCm'C
Cuts at: 1790 4110 5266 1790
Size: 2320 1156 2573
Fragments arranged by size:
2573 2320 1156
BmgBI CAC'GTC
Cuts at: 1318 2056 1318
Size: 738 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 3335 3780
Size: 416 53 83 399 24 972 946 445
Cuts at: 3780 4738 442
Size: 958 1753
Fragments arranged by size:
1753 972 958 946 445 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 2528 4709 1239
Size: 1268 21 2181 2579
Fragments arranged by size:
2579 2181 1268 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3331 4174 5039 5337 3331
Size: 843 865 298 4043
Fragments arranged by size:
4043 865 843 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2272 3169 4712 882
Size: 617 773 897 1543 2219
Fragments arranged by size:
2219 1543 897 773 617
BsaAI yAC'GTr
Cuts at: 493 1204 3133 3840 493
Size: 711 1929 707 2702
Fragments arranged by size:
2702 1929 711 707
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2873 3856 4238 4873
Size: 53 83 186 936 1340 983 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1451 1340 983 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 2499 4482 4880 5154 5301 5712 2124
Size: 375 1983 398 274 147 411 2461
Fragments arranged by size:
2461 1983 411 398 375 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5824
Fragments arranged by size:
5824 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3227 1186
Size: 2041 4008
BseYI C'CCAG_C
Cuts at: 3071 5252 5748 3071
Size: 2181 496 3372
Fragments arranged by size:
3372 2181 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3499 4260 5288 1540
Size: 275 186 811 687 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 687 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2493 4110 4195 5266 2012
Size: 481 1617 85 1071 2795
Fragments arranged by size:
2795 1617 1071 481 85
BsiWI C'GTAC_G
Cuts at: 2919 2919
Size: 6049
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2493 3368 4110 4195 5266 1391
Size: 399 222 481 875 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 742 481 399 222 85
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2460 3404 844
Size: 1271 345 944 3489
Fragments arranged by size:
3489 1271 944 345
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 3936 1394
Size: 429 202 1911 3507
Fragments arranged by size:
3507 1911 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 4544 4718 65
Size: 1183 1428 1868 174 1396
Fragments arranged by size:
1868 1428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2869 4693 4880 1700
Size: 110 1059 1824 187 2869
Fragments arranged by size:
2869 1824 1059 187 110
BsrGI T'GTAC_A
Cuts at: 96 3099 96
Size: 3003 3046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 2215 4109 5121 2215
Size: 1894 1012 3143
Fragments arranged by size:
3143 1894 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2095 2289 2502 3294 3379 3746 4131 4148
Size: 123 194 213 792 85 367 385 17
Cuts at: 4148 5600 5686 5698 6044 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 792 385 367 346 213 194
123 86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3024 3080 513
Size: 552 1959 56 3482
Fragments arranged by size:
3482 1959 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 3565 4359 4379 3565
Size: 794 20 5235
Fragments arranged by size:
5235 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2059 3329 2059
Size: 1270 4779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3041 3161 5056 809
Size: 507 1725 120 1895 1802
Fragments arranged by size:
1895 1802 1725 507 120
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3496 4385 6014
Size: 54 1425 50 275 18 1666 889 1629
Cuts at: 6014 8
Size: 43
Fragments arranged by size:
1666 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 3058 3979 1353
Size: 1382 323 921 3423
Fragments arranged by size:
3423 1382 921 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 6049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2432 2507 2528 2849 2990 3243
Size: 393 464 336 75 21 321 141 253
Cuts at: 3243 4112 4709 5496 5764 1239
Size: 869 597 787 268 1524
Fragments arranged by size:
1524 869 787 597 464 393 336 321
268 253 141 75 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3185 3185
Size: 6049
EcoO109I rG'GnC_Cy
Cuts at: 3013 3319 3013
Size: 306 5743
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2087 5196 1678
Size: 409 3109 2531
Fragments arranged by size:
3109 2531 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2744 2776 3119
Size: 32 135 32 816 32 324 32 343
Cuts at: 3119 3151 4690 4722 4764 4796 1373
Size: 32 1539 32 42 32 2626
Fragments arranged by size:
2626 1539 816 343 324 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 3393 3639 669
Size: 988 281 463 992 246 3079
Fragments arranged by size:
3079 992 988 463 281 246
HindIII A'AGCT_T
Cuts at: 748 2414 748
Size: 1666 4383
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2493 2787 5163 5347 1305
Size: 1188 294 2376 184 2007
Fragments arranged by size:
2376 2007 1188 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4227
Fragments arranged by size:
4227 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 4007 4366 4525 518
Size: 648 1071 1770 359 159 2042
Fragments arranged by size:
2042 1770 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2482 2698 2992 3465 4142 5290 5535
Size: 510 366 216 294 473 677 1148 245
Cuts at: 5535 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 510 473 366 294 245
216
NaeI GCC'GGC
Cuts at: 2871 2871
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5497
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NgoMIV G'CCGG_C
Cuts at: 2869 2869
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 3407 4952 1132
Size: 97 2178 1545 2229
Fragments arranged by size:
2229 2178 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2485 1108
Size: 1377 4672
PfoI T'CCnGG_A
Cuts at: 2505 2547 2703 2712 2505
Size: 42 156 9 5842
Fragments arranged by size:
5842 156 42 9
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3013 3319 3013
Size: 306 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 6017
PstI C_TGCA'G
Cuts at: 830 3173 3401 830
Size: 2343 228 3478
Fragments arranged by size:
3478 2343 228
PvuI CG_AT'CG
Cuts at: 2001 2812 2001
Size: 811 5238
PvuII CAG'CTG
Cuts at: 2116 2116
Size: 6049
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 3186 3186
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 2902 3169 3247 3397 4532 5213 5404
Size: 219 1857 267 78 150 1135 681 191
Cuts at: 5404 826
Size: 1471
Fragments arranged by size:
1857 1471 1135 681 267 219 191 150
78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 2869 1700
Size: 1169 4880
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3310 4153 5054 5316 820
Size: 197 926 1367 843 901 262 1553
Fragments arranged by size:
1553 1367 926 901 843 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 3488 513
Size: 552 2423 3074
Fragments arranged by size:
3074 2423 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2822 3317 4076 4093 4246 4431 4876
Size: 1255 1242 495 759 17 153 185 445
Cuts at: 4876 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 759 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3099 4295 6007
Size: 274 80 33 51 1443 1122 1196 1712
Cuts at: 6007 96
Size: 138
Fragments arranged by size:
1712 1443 1196 1122 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 6009 6041 2377
Size: 32 3600 32 2385
Fragments arranged by size:
3600 2385 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 2092 3385 2092
Size: 1293 4756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3299 3299
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI