Restriction map for
pFN21AE1038
(Circular) (Six-base) MAPSORT of: pFN21AE1038.seq Check: 3402 from: 1 to: 6721
pFN21AE1038 6721 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 2029
Size: 6721
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3579 3612 4784 6168 6436 1632
Size: 1947 33 1172 1384 268 1917
Fragments arranged by size:
1947 1917 1384 1172 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 2267 4464 6036 2267
Size: 2197 1572 2952
Fragments arranged by size:
2952 2197 1572
ApaLI G'TGCA_C
Cuts at: 4778 5934 4778
Size: 1156 5565
ApoI r'AATT_y
Cuts at: 1493 2247 2697 3027 3155 3271 3429 4030 4256
Size: 754 450 330 128 116 158 601 226
Cuts at: 4256 4408 6517 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 754 601 450 330 226 158
152 128 116
AseI AT'TA_AT
Cuts at: 160 1032 3006 5275 160
Size: 872 1974 2269 1606
Fragments arranged by size:
2269 1974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 4046 1870
Size: 73 246 1857 4545
Fragments arranged by size:
4545 1857 246 73
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3342 4042 5497 618
Size: 325 217 627 1555 700 1455 1842
Fragments arranged by size:
1842 1555 1455 700 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2967 4040 1391
Size: 1576 1073 4072
Fragments arranged by size:
4072 1576 1073
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2066 2625 3093 928
Size: 971 167 559 468 4556
Fragments arranged by size:
4556 971 559 468 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5823 4613
Size: 1210 5511
BclI T'GATC_A
Cuts at: 1548 2848 3505 1548
Size: 1300 657 4764
Fragments arranged by size:
4764 1300 657
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3399 5332 136
Size: 107 122 71 631 605 1727 1933 1525
Fragments arranged by size:
1933 1727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6716 6716
Size: 6721
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 4782 5938 1790
Size: 2992 1156 2573
Fragments arranged by size:
2992 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 4452 5410
Size: 416 53 83 399 24 808 2227 958
Cuts at: 5410 442
Size: 1753
Fragments arranged by size:
2227 1753 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2634 3594 3863 5381 1239
Size: 1395 960 269 1518 2579
Fragments arranged by size:
2579 1518 1395 960 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 3356 4846 5711 6009 2068
Size: 1288 1490 865 298 2780
Fragments arranged by size:
2780 1490 1288 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5384 882
Size: 617 3885 2219
Fragments arranged by size:
3885 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4512 493
Size: 711 3308 2702
Fragments arranged by size:
3308 2702 711
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4528 4910 5545 275
Size: 53 83 186 936 2995 382 635 1451
Fragments arranged by size:
2995 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5154 5552 5826 5973 6384 5154
Size: 398 274 147 411 5491
Fragments arranged by size:
5491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3609 3639 3678 3708 3836
Size: 30 165 30 2206 30 39 30 128
Cuts at: 3836 3866 1178
Size: 30 4033
Fragments arranged by size:
4033 2206 165 128 39 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2733 1186
Size: 1547 5174
BseYI C'CCAG_C
Cuts at: 2940 5924 6420 2940
Size: 2984 496 3241
Fragments arranged by size:
3241 2984 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3893 3981 1163
Size: 573 2157 88 3903
Fragments arranged by size:
3903 2157 573 88
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4171 4932 5960 1540
Size: 275 186 2170 761 1028 2301
Fragments arranged by size:
2301 2170 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 4782 4867 5938 2520
Size: 2262 85 1071 3303
Fragments arranged by size:
3303 2262 1071 85
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 2967 4040 4782 4867 5938 1391
Size: 399 730 447 1073 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 730 447 399 85
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 2122 3508 3907 4610 6340 2122
Size: 1386 399 703 1730 2503
Fragments arranged by size:
2503 1730 1386 703 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 4003 4076 844
Size: 1185 1974 73 3489
Fragments arranged by size:
3489 1974 1185 73
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5216 5390 65
Size: 1183 3968 174 1396
Fragments arranged by size:
3968 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6721
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2008 2534 4051 4418 4803 4820 6272 6358
Size: 36 526 1517 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1517 1452 526 385 367 346 86
36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2238 2238
Size: 6721
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5230
Fragments arranged by size:
5230 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 4237 5031 5051 2178
Size: 2059 794 20 3848
Fragments arranged by size:
3848 2059 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 2019 3700 3822 3891 4025 2019
Size: 1681 122 69 134 4715
Fragments arranged by size:
4715 1681 134 122 69
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4168 5057 6686
Size: 54 1425 50 275 18 2338 889 1629
Cuts at: 6686 8
Size: 43
Fragments arranged by size:
2338 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3951 4651 1353
Size: 2598 700 3423
Fragments arranged by size:
3423 2598 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3443 5308 5682 5828 387
Size: 1064 138 1854 1865 374 146 1280
Fragments arranged by size:
1865 1854 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2634 3579 3594 3612 3863 4784 5381
Size: 393 1002 945 15 18 251 921 597
Cuts at: 5381 6168 6436 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1002 945 921 787 597 393 268
251 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3612 3612
Size: 6721
EcoRI G'AATT_C
Cuts at: 3429 4030 4408 3429
Size: 601 378 5742
Fragments arranged by size:
5742 601 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2792 3758 2792
Size: 966 5755
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 5868 1678
Size: 4190 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 3293 3325 5362
Size: 32 135 32 639 32 1050 32 2037
Cuts at: 5362 5394 5436 5468 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2037 1050 639 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4065 4311 669
Size: 988 281 2127 246 3079
Fragments arranged by size:
3079 2127 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 3384 4320 3384
Size: 936 5785
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3030 5835 6019 1305
Size: 1725 2805 184 2007
Fragments arranged by size:
2805 2007 1725 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4899
Fragments arranged by size:
4899 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2400 4679 5038 5197 518
Size: 648 1234 2279 359 159 2042
Fragments arranged by size:
2279 2042 1234 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3297 4137 4814 5962 6207 1606
Size: 1691 840 677 1148 245 2120
Fragments arranged by size:
2120 1691 1148 840 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5230
Fragments arranged by size:
5230 939 552
NdeI CA'TA_TG
Cuts at: 387 3415 387
Size: 3028 3693
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 4079 5624 1132
Size: 97 2850 1545 2229
Fragments arranged by size:
2850 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6721
PfoI T'CCnGG_A
Cuts at: 2563 2563
Size: 6721
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6721
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2823 2855 2823
Size: 32 6689
PstI C_TGCA'G
Cuts at: 830 2040 2263 4073 830
Size: 1210 223 1810 3478
Fragments arranged by size:
3478 1810 1210 223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 3297 3297
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SfcI C'TryA_G
Cuts at: 826 1045 2036 2259 3676 3971 4069 5204 5885
Size: 219 991 223 1417 295 98 1135 681
Cuts at: 5885 6076 826
Size: 191 1471
Fragments arranged by size:
1471 1417 1135 991 681 295 223 219
191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 2191 4048 1872
Size: 319 1857 4545
Fragments arranged by size:
4545 1857 319
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2083 3371 4825 5726 5988 820
Size: 197 926 140 1288 1454 901 262 1553
Fragments arranged by size:
1553 1454 1288 926 901 262 197 140
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6721
StyI C'CwwG_G
Cuts at: 513 1065 2004 4160 513
Size: 552 939 2156 3074
Fragments arranged by size:
3074 2156 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2744 3562 3849 4748 4765 4918 5103
Size: 1255 1164 818 287 899 17 153 185
Cuts at: 5103 5548 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1164 899 818 445 287 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4967 6679 96
Size: 274 80 33 51 1443 2990 1712 138
Fragments arranged by size:
2990 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2526 2558 3609 3641 6681 6713 2526
Size: 32 1051 32 3040 32 2534
Fragments arranged by size:
3040 2534 1051 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 3652 3952 4057 3652
Size: 300 105 6316
Fragments arranged by size:
6316 300 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2688 2688
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 2189 4046 1870
Size: 319 1857 4545
Fragments arranged by size:
4545 1857 319
XmnI GAAnn'nnTTC
Cuts at: 2835 4850 2835
Size: 2015 4706
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI