Restriction map for
pFN21AE1037
(Circular) (Six-base) MAPSORT of: pFN21AE1037.seq Check: 4033 from: 1 to: 5866
pFN21AE1037 5866 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3165 3993 4366 1563
Size: 1602 828 373 3063
Fragments arranged by size:
3063 1602 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 2966 3929 5313 5581 1632
Size: 665 669 963 1384 268 1917
Fragments arranged by size:
1917 1384 963 669 665 268
AflII C'TTAA_G
Cuts at: 820 1017 3139 820
Size: 197 2122 3547
Fragments arranged by size:
3547 2122 197
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 3071 3609 5181 3071
Size: 538 1572 3756
Fragments arranged by size:
3756 1572 538
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2258 3175 3401 3553 5662 1493
Size: 765 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 765 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2047 2357 2364 3185 1391
Size: 656 310 7 821 4072
Fragments arranged by size:
4072 821 656 310 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BfrBI ATG'CAT
Cuts at: 2414 2414
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 2600 3283 2600
Size: 683 5183
Bme1580I G_kGCm'C
Cuts at: 1790 3927 5083 1790
Size: 2137 1156 2573
Fragments arranged by size:
2573 2137 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 2601 3118
Size: 416 53 83 399 24 942 242 517
Cuts at: 3118 3597 4555 442
Size: 479 958 1753
Fragments arranged by size:
1753 958 942 517 479 416 399 242
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 2330
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 4526 1239
Size: 1109 2178 2579
Fragments arranged by size:
2579 2178 1109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3991 4856 5154 2420
Size: 1571 865 298 3132
Fragments arranged by size:
3132 1571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 2057 5860 2057
Size: 3803 2063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2462 4299 4697 4971 5118 5529 2462
Size: 1837 398 274 147 411 2799
Fragments arranged by size:
2799 1837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5866
BseYI C'CCAG_C
Cuts at: 2092 5069 5565 2092
Size: 2977 496 2393
Fragments arranged by size:
2977 2393 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2364 3927 4012 5083 2126
Size: 238 1563 85 1071 2909
Fragments arranged by size:
2909 1563 1071 238 85
BsmI GAATG_Cn'
Cuts at: 2345 2747 3377 3470 2345
Size: 402 630 93 4741
Fragments arranged by size:
4741 630 402 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2126 2357 2364 3185 3927 4012
Size: 399 257 79 231 7 821 742 85
Cuts at: 4012 5083 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 821 742 399 257 231 85
79 7
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 2964 3755 5485 2964
Size: 791 1730 3345
Fragments arranged by size:
3345 1730 791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 2226 2815 96
Size: 2130 589 3147
Fragments arranged by size:
3147 2130 589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstXI CCAn_nnnn'nTGG
Cuts at: 2100 2100
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2058 3196 3563 3948 3965 5417 5503 5515
Size: 86 1138 367 385 17 1452 86 12
Cuts at: 5515 5861 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 86 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2742 2742
Size: 5866
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 3382 4176 4196 2656
Size: 726 794 20 4326
Fragments arranged by size:
4326 794 726 20
ClaI AT'CG_AT
Cuts at: 1554 2565 1554
Size: 1011 4855
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2559 2594 529
Size: 35 1995 35 3801
Fragments arranged by size:
3801 1995 35 35
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3313 4202
Size: 54 1425 50 275 18 1216 267 889
Cuts at: 4202 5831 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3796 1353
Size: 720 1723 3423
Fragments arranged by size:
3423 1723 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2348 2966 3929 4526 5313 5581
Size: 393 665 51 618 963 597 787 268
Cuts at: 5581 1239
Size: 1524
Fragments arranged by size:
1524 963 787 665 618 597 393 268
51
EcoRI G'AATT_C
Cuts at: 2258 3175 3553 2258
Size: 917 378 4571
Fragments arranged by size:
4571 917 378
EcoRV GAT'ATC
Cuts at: 3078 3078
Size: 5866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 3007 2620
Size: 387 5479
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 3037 5013 1678
Size: 1359 1976 2531
Fragments arranged by size:
2531 1976 1359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 4507 4539 4581
Size: 32 135 32 494 32 2409 32 42
Cuts at: 4581 4613 1373
Size: 32 2626
Fragments arranged by size:
2626 2409 494 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2959 3210 3456 669
Size: 988 281 1021 251 246 3079
Fragments arranged by size:
3079 1021 988 281 251 246
HindIII A'AGCT_T
Cuts at: 748 2602 748
Size: 1854 4012
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4980 5164 1305
Size: 3675 184 2007
Fragments arranged by size:
3675 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2419 2792 3824 4183 4342 518
Size: 648 1087 166 373 1032 359 159 2042
Fragments arranged by size:
2042 1087 1032 648 373 359 166 159
MspA1I CmG'CkG
Cuts at: 1606 2096 3282 3959 5107 5352 1606
Size: 490 1186 677 1148 245 2120
Fragments arranged by size:
2120 1186 1148 677 490 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NsiI A_TGCA'T
Cuts at: 2416 2416
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2414 3224 4769 1132
Size: 97 1185 810 1545 2229
Fragments arranged by size:
2229 1545 1185 810 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2115 1613
Size: 502 5364
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2096 2096
Size: 5866
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 3214 4349 5030 5221 826
Size: 219 2169 1135 681 191 1471
Fragments arranged by size:
2169 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 3139 3970 4871 5133 820
Size: 197 926 492 704 831 901 262 1553
Fragments arranged by size:
1553 926 901 831 704 492 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 2901 3790 5
Size: 47 2849 889 2081
Fragments arranged by size:
2849 2081 889 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 3305 513
Size: 552 939 1301 3074
Fragments arranged by size:
3074 1301 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 3910 4063 4248 4693 325
Size: 1255 2313 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2815 4112
Size: 274 80 33 51 1443 249 589 1297
Cuts at: 4112 5824 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1297 589 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 5826 5858 2602
Size: 32 3192 32 2610
Fragments arranged by size:
3192 2610 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3088 3202 3088
Size: 114 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2636 2636
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 2922 3995 2922
Size: 1073 4793
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI