Restriction map for
pFN21AE1035
(Circular) (Six-base) MAPSORT of: pFN21AE1035.seq Check: 613 from: 1 to: 5989
pFN21AE1035 5989 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2332 2332
Size: 5989
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 3332 1937
Size: 250 1145 4594
Fragments arranged by size:
4594 1145 250
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 2831 4052 5436 5704 1632
Size: 875 324 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 875 324 268
AfeI AGC'GCT
Cuts at: 2408 2408
Size: 5989
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 3173 1166
Size: 2007 3982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2747 1184
Size: 1563 4426
AlwNI CAG_nnn'CTG
Cuts at: 2069 2336 3732 5304 2069
Size: 267 1396 1572 2754
Fragments arranged by size:
2754 1572 1396 267
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2643 3021 3298 3524 3676 5785 1493
Size: 1150 378 277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1150 378 277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2370 3314 1870
Size: 73 427 944 4545
Fragments arranged by size:
4545 944 427 73
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3149 3310 4765 618
Size: 325 217 627 1362 161 1455 1842
Fragments arranged by size:
1842 1455 1362 627 325 217 161
BanII G_rGCy'C
Cuts at: 1391 2034 2117 3308 1391
Size: 643 83 1191 4072
Fragments arranged by size:
4072 1191 643 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BbvCI CC'TCA_GC
Cuts at: 2085 2085
Size: 5989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3043 3077 4180 4214 1903
Size: 34 1106 34 1103 34 3678
Fragments arranged by size:
3678 1106 1103 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2540 3881 5091 2540
Size: 1341 1210 3438
Fragments arranged by size:
3438 1341 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3147 4600 136
Size: 107 122 71 631 605 1475 1453 1525
Fragments arranged by size:
1525 1475 1453 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2748 5984 2748
Size: 3236 2753
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 4050 5206 1790
Size: 2260 1156 2573
Fragments arranged by size:
2573 2260 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2346 3720 4678
Size: 416 53 83 399 24 929 1374 958
Cuts at: 4678 442
Size: 1753
Fragments arranged by size:
1753 1374 958 929 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 3003 2428
Size: 575 5414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2505 4649 1239
Size: 1106 160 2144 2579
Fragments arranged by size:
2579 2144 1106 160
Bpu10I CC'TnA_GC
Cuts at: 2085 2085
Size: 5989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2716 2879 4114 4979 5277 2144
Size: 572 163 1235 865 298 2856
Fragments arranged by size:
2856 1235 865 572 298 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2803 3780 493
Size: 711 1599 977 2702
Fragments arranged by size:
2702 1599 977 711
BsaBI GATnn'nnATC
Cuts at: 3260 5983 3260
Size: 2723 3266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2829 4422 4820 5094 5241 5652 2829
Size: 1593 398 274 147 411 3166
Fragments arranged by size:
3166 1593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3251 3281 1178
Size: 30 165 30 1848 30 3886
Fragments arranged by size:
3886 1848 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2990 3256 1186
Size: 1804 266 3919
Fragments arranged by size:
3919 1804 266
BseYI C'CCAG_C
Cuts at: 2069 2916 2941 5192 5688 2069
Size: 847 25 2251 496 2370
Fragments arranged by size:
2370 2251 847 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 3233 1163
Size: 573 1179 318 3919
Fragments arranged by size:
3919 1179 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 4050 4135 5206 2034
Size: 2016 85 1071 2817
Fragments arranged by size:
2817 2016 1071 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
BsmBI CGTCTCn'nnnn_
Cuts at: 2759 2759
Size: 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2117 3308 4050 4135 5206 1391
Size: 399 244 83 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 244 85 83
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 2676 3878 5608 2676
Size: 1202 1730 3057
Fragments arranged by size:
3057 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 2662 3126 3344 844
Size: 1488 330 464 218 3489
Fragments arranged by size:
3489 1488 464 330 218
BsrBI CCG'CTC
Cuts at: 1394 1823 3067 3876 1394
Size: 429 1244 809 3507
Fragments arranged by size:
3507 1244 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 2946 3075 4484 4658 65
Size: 1183 1656 42 129 1409 174 1396
Fragments arranged by size:
1656 1409 1396 1183 174 129 42
BsrFI r'CCGG_y
Cuts at: 1700 1810 3141 4633 4820 1700
Size: 110 1331 1492 187 2869
Fragments arranged by size:
2869 1492 1331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 2663 4049 5061 2663
Size: 1386 1012 3591
Fragments arranged by size:
3591 1386 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2788 794
Size: 1994 3995
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstXI CCAn_nnnn'nTGG
Cuts at: 2138 3160 2138
Size: 1022 4967
BstYI r'GATC_y
Cuts at: 1972 2706 2737 2748 3198 3319 3686 4071 4088
Size: 734 31 11 450 121 367 385 17
Cuts at: 4088 5540 5626 5638 5984 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 734 450 385 367 346 121
86 31 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2235 3099 2235
Size: 864 5125
BtgI C'CryG_G
Cuts at: 513 1065 2004 2959 513
Size: 552 939 955 3543
Fragments arranged by size:
3543 955 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 2312 3505 4299 4319 2301
Size: 11 1193 794 20 3971
Fragments arranged by size:
3971 1193 794 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3004 3293 3004
Size: 289 5700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 3046 3129
Size: 54 1425 50 275 18 477 739 83
Cuts at: 3129 3436 4325 5954 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 889 739 477 307 275 83
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2472 3919 1353
Size: 1119 1447 3423
Fragments arranged by size:
3423 1447 1119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2505 2507 2831 4052 4649 5436
Size: 393 713 160 2 324 1221 597 787
Cuts at: 5436 5704 1239
Size: 268 1524
Fragments arranged by size:
1524 1221 787 713 597 393 324 268
160 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2264 2264
Size: 5989
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 5989
EcoRI G'AATT_C
Cuts at: 3021 3298 3676 3021
Size: 277 378 5334
Fragments arranged by size:
5334 378 277
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2410 5136 1678
Size: 732 2726 2531
Fragments arranged by size:
2726 2531 732
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 3251 3283 4630
Size: 32 135 32 620 32 1027 32 1347
Cuts at: 4630 4662 4704 4736 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1347 1027 620 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 2775 3333 3579 669
Size: 988 281 250 587 558 246 3079
Fragments arranged by size:
3079 988 587 558 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5103 5287 1305
Size: 3798 184 2007
Fragments arranged by size:
3798 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 3131 10
Size: 54 1425 343 477 822 2868
Fragments arranged by size:
2868 1425 822 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3173 3947 4306 4465 518
Size: 648 2007 774 359 159 2042
Fragments arranged by size:
2042 2007 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2323 2899 3405 4082 5230 5475 1606
Size: 463 254 576 506 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 506 463 254 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2425 2626 3097 3347 4892 1132
Size: 97 1196 201 471 250 1545 2229
Fragments arranged by size:
2229 1545 1196 471 250 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2336 1108
Size: 1228 4761
PfoI T'CCnGG_A
Cuts at: 2637 2637
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2625 1613
Size: 1012 4977
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 3341 830
Size: 2511 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2069 2323 2069
Size: 254 5735
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 2186 3331 1936
Size: 250 1145 4594
Fragments arranged by size:
4594 1145 250
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 2798 4237 5949 2798
Size: 1439 1712 2838
Fragments arranged by size:
2838 1712 1439
SexAI A'CCwGG_T
Cuts at: 2868 2868
Size: 5989
SfcI C'TryA_G
Cuts at: 826 1045 3337 4472 5153 5344 826
Size: 219 2292 1135 681 191 1471
Fragments arranged by size:
2292 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2695 2858 4093 4994 5256
Size: 197 926 216 536 163 1235 901 262
Cuts at: 5256 820
Size: 1553
Fragments arranged by size:
1553 1235 926 901 536 262 216 197
163
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 2923 152
Size: 2771 3218
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3235 3913 5
Size: 47 3183 678 2081
Fragments arranged by size:
3183 2081 678 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2910 3153 3428 513
Size: 552 939 906 243 275 3074
Fragments arranged by size:
3074 939 906 552 275 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4016 4033 4186 4371 4816 325
Size: 1255 2436 17 153 185 445 1498
Fragments arranged by size:
2436 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 4235 5947
Size: 274 80 33 51 1443 819 1439 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1712 1443 1439 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 5949 5981 2246
Size: 32 3671 32 2254
Fragments arranged by size:
3671 2254 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2278 2248
Size: 30 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 2646 4118 2646
Size: 1472 4517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI