Restriction map for
pFN21AE1034
(Circular) (Six-base) MAPSORT of: pFN21AE1034.seq Check: 3622 from: 1 to: 5977
pFN21AE1034 5977 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2396 2396
Size: 5977
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 3276 4104 4477 1563
Size: 1713 828 373 3063
Fragments arranged by size:
3063 1713 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3206 4040 5424 5692 1632
Size: 1172 402 834 1384 268 1917
Fragments arranged by size:
1917 1384 1172 834 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2014 2323 3720 5292 2014
Size: 309 1397 1572 2699
Fragments arranged by size:
2699 1572 1397 309
ApaLI G'TGCA_C
Cuts at: 2126 4034 5190 2126
Size: 1908 1156 2913
Fragments arranged by size:
2913 1908 1156
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2708 3132 3298 4753
Size: 325 217 627 303 618 424 166 1455
Cuts at: 4753 618
Size: 1842
Fragments arranged by size:
1842 1455 627 618 424 325 303 217
166
BanII G_rGCy'C
Cuts at: 1391 2644 3296 1391
Size: 1253 652 4072
Fragments arranged by size:
4072 1253 652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4168 4202 1903
Size: 34 50 34 2147 34 3678
Fragments arranged by size:
3678 2147 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2596 2756 3869 5079 2596
Size: 160 1113 1210 3494
Fragments arranged by size:
3494 1210 1113 160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2157 2166
Size: 107 122 71 631 605 427 58 9
Cuts at: 2166 4588 136
Size: 2422 1525
Fragments arranged by size:
2422 1525 631 605 427 122 107 71
58 9
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2571 3394 2571
Size: 823 5154
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2130 2711 3135 3157 4038 5194 1790
Size: 305 35 581 424 22 881 1156 2573
Fragments arranged by size:
2573 1156 881 581 424 305 35 22
BmgBI CAC'GTC
Cuts at: 1318 2116 2230 3019 1318
Size: 798 114 789 4276
Fragments arranged by size:
4276 798 789 114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2975 3149
Size: 416 53 83 399 24 894 664 174
Cuts at: 3149 3159 3708 4666 442
Size: 10 549 958 1753
Fragments arranged by size:
1753 958 894 664 549 416 399 174
83 53 24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
Bpu10I CC'TnA_GC
Cuts at: 2084 2084
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 4102 4967 5265 2207
Size: 1895 865 298 2919
Fragments arranged by size:
2919 1895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 1178
Size: 30 165 30 1044 30 4678
Fragments arranged by size:
4678 1044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2731 1186
Size: 1545 4432
BseYI C'CCAG_C
Cuts at: 2026 2251 2622 2753 5180 5676 2026
Size: 225 371 131 2427 496 2327
Fragments arranged by size:
2427 2327 496 371 225 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3130 3138 1163
Size: 573 1394 8 4002
Fragments arranged by size:
4002 1394 573 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2109 3427 4188 5216 1540
Size: 275 186 48 60 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186 60 48
BsiHKAI G_wGCw'C
Cuts at: 2130 4038 4123 5194 2130
Size: 1908 85 1071 2913
Fragments arranged by size:
2913 1908 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2130 2644 2711 3135 3157 3296
Size: 399 305 35 514 67 424 22 139
Cuts at: 3296 4038 4123 5194 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 424 399 305 139
85 67 35 22
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2208 3866 5596 2208
Size: 1658 1730 2589
Fragments arranged by size:
2589 1730 1658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 2396 2974 3332 844
Size: 1164 388 578 358 3489
Fragments arranged by size:
3489 1164 578 388 358
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 2575 3864 1394
Size: 429 265 487 1289 3507
Fragments arranged by size:
3507 1289 487 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 2023 794
Size: 1220 9 4748
Fragments arranged by size:
4748 1220 9
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2863 2467
Size: 396 5581
BstYI r'GATC_y
Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972
Size: 1335 367 385 17 1452 86 12 346
Cuts at: 5972 1972
Size: 1977
Fragments arranged by size:
1977 1452 1335 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2036 2915 3127 2036
Size: 879 212 4886
Fragments arranged by size:
4886 879 212
BtgI C'CryG_G
Cuts at: 513 1065 2109 2161 2214 2688 2763 3193 513
Size: 552 1044 52 53 474 75 430 3297
Fragments arranged by size:
3297 1044 552 474 430 75 53 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 2934 3126 3493 4287 4307 2397
Size: 537 192 367 794 20 4067
Fragments arranged by size:
4067 794 537 367 192 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 4984 809
Size: 507 948 2720 1802
Fragments arranged by size:
2720 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2106 2142
Size: 54 1425 50 275 18 216 60 36
Cuts at: 2142 2394 2775 3424 4313 5942 8
Size: 252 381 649 889 1629 43
Fragments arranged by size:
1629 1425 889 649 381 275 252 216
60 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2106 3424 1537
Size: 275 234 60 1318 4090
Fragments arranged by size:
4090 1318 275 234 60
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3194 3907 1353
Size: 1527 314 713 3423
Fragments arranged by size:
3423 1527 713 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 4564 4938 5084 387
Size: 1064 138 1149 1826 374 146 1280
Fragments arranged by size:
1826 1280 1149 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2804 3206 4040 4637 5424 5692 1239
Size: 393 1172 402 834 597 787 268 1524
Fragments arranged by size:
1524 1172 834 787 597 402 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2770 2911 2938 2770
Size: 141 27 5809
Fragments arranged by size:
5809 141 27
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 3193 5124 1678
Size: 1515 1931 2531
Fragments arranged by size:
2531 1931 1515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2479 3150 3182 4618
Size: 32 135 32 875 32 671 32 1436
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1436 875 671 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 5091 5275 1305
Size: 943 2843 184 2007
Fragments arranged by size:
2843 2007 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2777 10
Size: 54 1425 343 564 381 3210
Fragments arranged by size:
3210 1425 564 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 2726 2762 3935 4294 4453 518
Size: 648 1272 288 36 1173 359 159 2042
Fragments arranged by size:
2042 1272 1173 648 359 288 159 36
MspA1I CmG'CkG
Cuts at: 1606 2111 2163 2216 2251 2819 2852 3251 3393
Size: 505 52 53 35 568 33 399 142
Cuts at: 3393 4070 5218 5463 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 568 505 399 245 142
53 52 35 33
NcoI C'CATG_G
Cuts at: 513 1065 2763 513
Size: 552 1698 3727
Fragments arranged by size:
3727 1698 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3335 4880 1132
Size: 97 2106 1545 2229
Fragments arranged by size:
2229 2106 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3109 3269 1348
Size: 1761 160 4056
Fragments arranged by size:
4056 1761 160
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 2938 2911
Size: 27 5950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2019 2283 3329 830
Size: 1189 264 1046 3478
Fragments arranged by size:
3478 1189 1046 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2251 2819 2852 2251
Size: 568 33 5376
Fragments arranged by size:
5376 568 33
SacII CC_GC'GG
Cuts at: 2112 2164 2217 2112
Size: 52 53 5872
Fragments arranged by size:
5872 53 52
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 2938 2938
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2015 2279 2384 3325 4460 5141 5332
Size: 219 970 264 105 941 1135 681 191
Cuts at: 5332 826
Size: 1471
Fragments arranged by size:
1471 1135 970 941 681 264 219 191
105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 4081 4982 5244 820
Size: 197 926 279 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 2494 3901 5
Size: 47 2442 1407 2081
Fragments arranged by size:
2442 2081 1407 47
StuI AGG'CCT
Cuts at: 1517 2102 3044 1517
Size: 585 942 4450
Fragments arranged by size:
4450 942 585
StyI C'CwwG_G
Cuts at: 513 1065 2763 2877 2905 3416 513
Size: 552 1698 114 28 511 3074
Fragments arranged by size:
3074 1698 552 511 114 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3150 4004 4021 4174 4359 4804 325
Size: 1255 1570 854 17 153 185 445 1498
Fragments arranged by size:
1570 1498 1255 854 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 2770 2840 2761
Size: 9 70 5898
Fragments arranged by size:
5898 70 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI