Restriction map for
pFN21AE1032
(Circular) (Six-base) MAPSORT of: pFN21AE1032.seq Check: 1903 from: 1 to: 8986
pFN21AE1032 8986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6796 278
Size: 53 83 186 936 5260 2468
Fragments arranged by size:
5260 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2632 6252 6329 1937
Size: 695 3620 77 4594
Fragments arranged by size:
4594 3620 695 77
Acc65I G'GTAC_C
Cuts at: 6307 6307
Size: 8986
AclI AA'CG_TT
Cuts at: 1563 7113 7486 1563
Size: 5550 373 3063
Fragments arranged by size:
5550 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 4113 4362 4482 5100 6030 7049 8433
Size: 1317 1164 249 120 618 930 1019 1384
Cuts at: 8433 8701 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1317 1164 1019 930 618 268
249 120
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8789
AflIII A'CryG_T
Cuts at: 1203 2922 5188 7885 8862 1203
Size: 1719 2266 2697 977 1327
Fragments arranged by size:
2697 2266 1719 1327 977
AgeI A'CCGG_T
Cuts at: 7817 7817
Size: 8986
AhdI GACnn_n'nnGTC
Cuts at: 7715 7715
Size: 8986
AleI CACnn'nnGTG
Cuts at: 1166 5187 1166
Size: 4021 4965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4951 1184
Size: 3767 5219
AlwNI CAG_nnn'CTG
Cuts at: 3926 4118 4265 4859 4931 6729 8301 3926
Size: 192 147 594 72 1798 1572 4611
Fragments arranged by size:
4611 1798 1572 594 192 147 72
ApaLI G'TGCA_C
Cuts at: 7043 8199 7043
Size: 1156 7830
ApoI r'AATT_y
Cuts at: 1493 2493 2695 2810 2894 2916 3247 3376 3661
Size: 1000 202 115 84 22 331 129 285
Cuts at: 3661 3726 3870 3976 4681 5551 6175 6257 6295
Size: 65 144 106 705 870 624 82 38
Cuts at: 6295 6521 6673 8782 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1000 870 705 624 331 285
226 202 152 144 129 115 106 84
82 65 38 22
AseI AT'TA_AT
Cuts at: 160 1032 3690 3750 4293 6008 7540 160
Size: 872 2658 60 543 1715 1532 1606
Fragments arranged by size:
2658 1715 1606 1532 872 543 60
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8986
AvaI C'yCGr_G
Cuts at: 1870 1943 6311 1870
Size: 73 4368 4545
Fragments arranged by size:
4545 4368 73
AvrII C'CTAG_G
Cuts at: 2899 2899
Size: 8986
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2457 2490 3282 3315 5667 5700 2457
Size: 33 792 33 2352 33 5743
Fragments arranged by size:
5743 2352 792 33 33 33
BamHI G'GATC_C
Cuts at: 6316 6683 6316
Size: 367 8619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6307 7762 618
Size: 325 217 627 4520 1455 1842
Fragments arranged by size:
4520 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2338 6305 1391
Size: 947 3967 4072
Fragments arranged by size:
4072 3967 947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3243 3468 3797 3885 4317 4470 5901
Size: 971 1344 225 329 88 432 153 1431
Cuts at: 5901 928
Size: 4013
Fragments arranged by size:
4013 1431 1344 971 432 329 225 153
88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7177 7211 1903
Size: 34 5240 34 3678
Fragments arranged by size:
5240 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4958 6878 8088 4958
Size: 1920 1210 5856
Fragments arranged by size:
5856 1920 1210
BclI T'GATC_A
Cuts at: 1548 3511 1548
Size: 1963 7023
BfrBI ATG'CAT
Cuts at: 4205 4205
Size: 8986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7597 136
Size: 107 122 71 631 605 5925 1525
Fragments arranged by size:
5925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5107 8981 5107
Size: 3874 5112
BlpI GC'TnA_GC
Cuts at: 6403 6403
Size: 8986
Bme1580I G_kGCm'C
Cuts at: 1790 5808 7047 8203 1790
Size: 4018 1239 1156 2573
Fragments arranged by size:
4018 2573 1239 1156
BmgBI CAC'GTC
Cuts at: 1318 5189 1318
Size: 3871 5115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4847 6219
Size: 416 53 83 399 24 1649 1781 1372
Cuts at: 6219 6717 7675 442
Size: 498 958 1753
Fragments arranged by size:
1781 1753 1649 1372 958 498 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2795 1056
Size: 1739 7247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3513 6163 3513
Size: 2650 6336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 2583 4056 4095 4797 6004 7646 1239
Size: 1143 201 1473 39 702 1207 1642 2579
Fragments arranged by size:
2579 1642 1473 1207 1143 702 201 39
Bpu10I CC'TnA_GC
Cuts at: 2592 2847 3957 4986 5821 2592
Size: 255 1110 1029 835 5757
Fragments arranged by size:
5757 1110 1029 835 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7111 7976 8274 7111
Size: 865 298 7823
Fragments arranged by size:
7823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7649 882
Size: 617 6150 2219
Fragments arranged by size:
6150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6777 493
Size: 711 5573 2702
Fragments arranged by size:
5573 2702 711
BsaBI GATnn'nnATC
Cuts at: 8980 8980
Size: 8986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4067 6793 7175 7810
Size: 53 83 186 936 2534 2726 382 635
Cuts at: 7810 275
Size: 1451
Fragments arranged by size:
2726 2534 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7419 7817 8091 8238 8649 7419
Size: 398 274 147 411 7756
Fragments arranged by size:
7756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2437 2467 5200 5230 1178
Size: 30 165 30 1034 30 2733 30 4934
Fragments arranged by size:
4934 2733 1034 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2442 4884 4887 5361 6045 1186
Size: 1256 2442 3 474 684 4127
Fragments arranged by size:
4127 2442 1256 684 474 3
BseYI C'CCAG_C
Cuts at: 2239 2971 8189 8685 2239
Size: 732 5218 496 2540
Fragments arranged by size:
5218 2540 732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2427 4671 1163
Size: 573 691 2244 5478
Fragments arranged by size:
5478 2244 691 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6436 7197 8225 1540
Size: 275 186 4435 761 1028 2301
Fragments arranged by size:
4435 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2593 6101 7047 7132 8203 2593
Size: 3508 946 85 1071 3376
Fragments arranged by size:
3508 3376 1071 946 85
BsmI GAATG_Cn'
Cuts at: 5281 6497 6590 5281
Size: 1216 93 7677
Fragments arranged by size:
7677 1216 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4222 4222
Size: 8986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 2593 5808 6101 6305 7047 7132
Size: 399 548 255 3215 293 204 742 85
Cuts at: 7132 8203 1391
Size: 1071 2174
Fragments arranged by size:
3215 2174 1071 742 548 399 293 255
204 85
BspEI T'CCGG_A
Cuts at: 8649 8649
Size: 8986
BspHI T'CATG_A
Cuts at: 6875 8605 6875
Size: 1730 7256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2081 4314 4894 6341 844
Size: 1237 2233 580 1447 3489
Fragments arranged by size:
3489 2233 1447 1237 580
BsrBI CCG'CTC
Cuts at: 1394 1823 6873 1394
Size: 429 5050 3507
Fragments arranged by size:
5050 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4500 6269 7481 7655 65
Size: 1183 3252 1769 1212 174 1396
Fragments arranged by size:
3252 1769 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5412 7630 7817 1700
Size: 110 3602 2218 187 2869
Fragments arranged by size:
3602 2869 2218 187 110
BsrGI T'GTAC_A
Cuts at: 96 2126 3164 96
Size: 2030 1038 5918
Fragments arranged by size:
5918 2030 1038
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8986
BssSI C'ACGA_G
Cuts at: 2751 7046 8058 2751
Size: 4295 1012 3679
Fragments arranged by size:
4295 3679 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8986
BstBI TT'CG_AA
Cuts at: 7790 7790
Size: 8986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8986
BstYI r'GATC_y
Cuts at: 1972 2500 2704 4790 5107 5689 6316 6683 7068
Size: 528 204 2086 317 582 627 367 385
Cuts at: 7068 7085 8537 8623 8635 8981 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2086 1977 1452 627 582 528 385 367
346 317 204 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5538 487
Size: 683 56 4312 3935
Fragments arranged by size:
4312 3935 683 56
BtsI GCAGTG_nn'
Cuts at: 5612 6502 7296 7316 5612
Size: 890 794 20 7282
Fragments arranged by size:
7282 890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5092 5127 529
Size: 35 4528 35 4388
Fragments arranged by size:
4528 4388 35 35
DraI TTT'AAA
Cuts at: 2106 2193 2574 3289 3849 6290 2106
Size: 87 381 715 560 2441 4802
Fragments arranged by size:
4802 2441 715 560 381 87
DraIII CAC_nnn'GTG
Cuts at: 3404 4529 3404
Size: 1125 7861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7993 809
Size: 507 6677 1802
Fragments arranged by size:
6677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2488 6433 7322
Size: 54 1425 50 275 18 658 3945 889
Cuts at: 7322 8951 8
Size: 1629 43
Fragments arranged by size:
3945 1629 1425 889 658 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6433 1537
Size: 275 4621 4090
Fragments arranged by size:
4621 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 3584 4002 4110 5145 6916 1353
Size: 1431 800 418 108 1035 1771 3423
Fragments arranged by size:
3423 1771 1431 1035 800 418 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5704 7573 7947 8093 387
Size: 1064 138 4115 1869 374 146 1280
Fragments arranged by size:
4115 1869 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2583 2949 4056 4095 4113 4362
Size: 393 750 201 366 1107 39 18 249
Cuts at: 4362 4482 4797 5100 6004 6030 7049 7646 8433
Size: 120 315 303 904 26 1019 597 787
Cuts at: 8433 8701 1239
Size: 268 1524
Fragments arranged by size:
1524 1107 1019 904 787 750 597 393
366 315 303 268 249 201 120 39
26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2460 4074 5010 5820 5878 2460
Size: 1614 936 810 58 5568
Fragments arranged by size:
5568 1614 936 810 58
EcoO109I rG'GnC_Cy
Cuts at: 5869 5869
Size: 8986
EcoRI G'AATT_C
Cuts at: 2894 3376 3976 6295 6673 2894
Size: 482 600 2319 378 5207
Fragments arranged by size:
5207 2319 600 482 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2682 4596 2682
Size: 1914 7072
FspI TGC'GCA
Cuts at: 7492 7492
Size: 8986
HaeII r_GCGC'y
Cuts at: 1678 8133 1678
Size: 6455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 3875 3907 3908
Size: 32 135 32 1556 32 715 32 1
Cuts at: 3908 3940 3977 4009 4412 4444 5178 5210 7627
Size: 32 37 32 403 32 734 32 2417
Cuts at: 7627 7659 7701 7733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2417 1556 734 715 403 135 42
37 32 32 32 32 32 32 32
32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 3053 4759 6330 6576 669
Size: 988 281 592 523 1706 1571 246 3079
Fragments arranged by size:
3079 1706 1571 988 592 523 281 246
HindIII A'AGCT_T
Cuts at: 748 2378 2568 6218 748
Size: 1630 190 3650 3516
Fragments arranged by size:
3650 3516 1630 190
HpaI GTT'AAC
Cuts at: 6576 6576
Size: 8986
KpnI G_GTAC'C
Cuts at: 6311 6311
Size: 8986
MfeI C'AATT_G
Cuts at: 2886 6585 2886
Size: 3699 5287
MluI A'CGCG_T
Cuts at: 8862 8862
Size: 8986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4652 4846 5038 8100 8284 1305
Size: 3347 194 192 3062 184 2007
Fragments arranged by size:
3347 3062 2007 194 192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 10
Size: 54 1425 343 658 6506
Fragments arranged by size:
6506 1425 658 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2257 3849 4933 5187 5738 6008 6279
Size: 648 1091 1592 1084 254 551 270 271
Cuts at: 6279 6944 7303 7462 518
Size: 665 359 159 2042
Fragments arranged by size:
2042 1592 1091 1084 665 648 551 359
271 270 254 159
MspA1I CmG'CkG
Cuts at: 1606 3296 5168 5681 6402 7079 8227 8472 1606
Size: 1690 1872 513 721 677 1148 245 2120
Fragments arranged by size:
2120 1872 1690 1148 721 677 513 245
NaeI GCC'GGC
Cuts at: 5414 5414
Size: 8986
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8434
NdeI CA'TA_TG
Cuts at: 387 4914 387
Size: 4527 4459
NgoMIV G'CCGG_C
Cuts at: 5412 5412
Size: 8986
NheI G'CTAG_C
Cuts at: 1052 2791 1052
Size: 1739 7247
NotI GC'GGCC_GC
Cuts at: 6433 6433
Size: 8986
NsiI A_TGCA'T
Cuts at: 4207 4207
Size: 8986
NspI r_CATG'y
Cuts at: 1132 1229 2302 2612 2926 6344 7889 1132
Size: 97 1073 310 314 3418 1545 2229
Fragments arranged by size:
3418 2229 1545 1073 314 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8986
PciI A'CATG_T
Cuts at: 2922 7885 2922
Size: 4963 4023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4837 5251 1108
Size: 3729 414 4843
Fragments arranged by size:
4843 3729 414
PmeI GTTT'AAAC
Cuts at: 6290 6290
Size: 8986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7055 7087 8568 8600 7055
Size: 32 1481 32 7441
Fragments arranged by size:
7441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8986
PsiI TTA'TAA
Cuts at: 6556 6556
Size: 8986
PstI C_TGCA'G
Cuts at: 830 4474 5954 6338 830
Size: 3644 1480 384 3478
Fragments arranged by size:
3644 3478 1480 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8986
PvuII CAG'CTG
Cuts at: 3296 5168 5681 3296
Size: 1872 513 6601
Fragments arranged by size:
6601 1872 513
SalI G'TCGA_C
Cuts at: 1936 6328 1936
Size: 4392 4594
SbfI CC_TGCA'GG
Cuts at: 6338 6338
Size: 8986
ScaI AGT'ACT
Cuts at: 7234 8946 7234
Size: 1712 7274
SfcI C'TryA_G
Cuts at: 826 1045 3450 4086 4389 4470 5604 5853 5950
Size: 219 2405 636 303 81 1134 249 97
Cuts at: 5950 6334 7469 8150 8341 826
Size: 384 1135 681 191 1471
Fragments arranged by size:
2405 1471 1135 1134 681 636 384 303
249 219 191 97 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8986
SmaI CCC'GGG
Cuts at: 1872 6313 1872
Size: 4441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7090 7991 8253 820
Size: 197 926 5147 901 262 1553
Fragments arranged by size:
5147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6777 493
Size: 6284 2702
SpeI A'CTAG_T
Cuts at: 152 2819 4360 152
Size: 2667 1541 4778
Fragments arranged by size:
4778 2667 1541
SphI G_CATG'C
Cuts at: 1132 2302 6344 1132
Size: 1170 4042 3774
Fragments arranged by size:
4042 3774 1170
SspI AAT'ATT
Cuts at: 5 52 2833 6910 5
Size: 47 2781 4077 2081
Fragments arranged by size:
4077 2781 2081 47
StuI AGG'CCT
Cuts at: 1517 3284 1517
Size: 1767 7219
StyI C'CwwG_G
Cuts at: 513 1065 2312 2899 4536 4733 4831 6425 513
Size: 552 1247 587 1637 197 98 1594 3074
Fragments arranged by size:
3074 1637 1594 1247 587 552 197 98
SwaI ATTT'AAAT
Cuts at: 3849 3849
Size: 8986
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5320 7013 7030 7183 7368 7813 325
Size: 1255 3740 1693 17 153 185 445 1498
Fragments arranged by size:
3740 1693 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2126 3164 4825
Size: 274 80 33 51 1443 149 1038 1661
Cuts at: 4825 5191 5662 5903 7232 8944 96
Size: 366 471 241 1329 1712 138
Fragments arranged by size:
1712 1661 1443 1329 1038 471 366 274
241 149 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4136 4168 5200 5232 8946 8978 4136
Size: 32 1032 32 3714 32 4144
Fragments arranged by size:
4144 3714 1032 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8986
XbaI T'CTAG_A
Cuts at: 6322 6322
Size: 8986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3055
Size: 8986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8986
XmaI C'CCGG_G
Cuts at: 1870 6311 1870
Size: 4441 4545
XmnI GAAnn'nnTTC
Cuts at: 7115 7115
Size: 8986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6794 276
Size: 53 83 186 936 5260 2468
Fragments arranged by size:
5260 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI
NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI