Restriction map for
pFN21AE1030
(Circular) (Six-base) MAPSORT of: pFN21AE1030.seq Check: 4773 from: 1 to: 8605
pFN21AE1030 8605 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6415 278
Size: 53 83 186 936 4879 2468
Fragments arranged by size:
4879 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2919 3286 5948 1937
Size: 982 367 2662 4594
Fragments arranged by size:
4594 2662 982 367
Acc65I G'GTAC_C
Cuts at: 2560 5926 2560
Size: 3366 5239
AclI AA'CG_TT
Cuts at: 1563 6732 7105 1563
Size: 5169 373 3063
Fragments arranged by size:
5169 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 2837 2865 2916 3783 3882 3972 4583
Size: 861 344 28 51 867 99 90 611
Cuts at: 4583 5229 5454 5834 6668 8052 8320 1632
Size: 646 225 380 834 1384 268 1917
Fragments arranged by size:
1917 1384 867 861 834 646 611 380
344 268 225 99 90 51 28
AflII C'TTAA_G
Cuts at: 820 1017 3914 4400 820
Size: 197 2897 486 5025
Fragments arranged by size:
5025 2897 486 197
AflIII A'CryG_T
Cuts at: 1203 3187 4732 7504 8481 1203
Size: 1984 1545 2772 977 1327
Fragments arranged by size:
2772 1984 1545 1327 977
AgeI A'CCGG_T
Cuts at: 7436 7436
Size: 8605
AhdI GACnn_n'nnGTC
Cuts at: 7334 7334
Size: 8605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4008 1184
Size: 2824 5781
AlwNI CAG_nnn'CTG
Cuts at: 3598 3973 5701 6348 7920 3598
Size: 375 1728 647 1572 4283
Fragments arranged by size:
4283 1728 1572 647 375
ApaLI G'TGCA_C
Cuts at: 6662 7818 6662
Size: 1156 7449
ApoI r'AATT_y
Cuts at: 1493 3566 3756 3934 4080 4100 4318 5361 5914
Size: 2073 190 178 146 20 218 1043 553
Cuts at: 5914 6140 6292 8401 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 2073 1697 1043 553 226 218 190
178 152 146 20
AseI AT'TA_AT
Cuts at: 160 1032 7159 160
Size: 872 6127 1606
Fragments arranged by size:
6127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
AvaI C'yCGr_G
Cuts at: 1870 1943 2247 5930 1870
Size: 73 304 3683 4545
Fragments arranged by size:
4545 3683 304 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3609 3642 3609
Size: 33 8572
BamHI G'GATC_C
Cuts at: 2790 5935 6302 2790
Size: 3145 367 5093
Fragments arranged by size:
5093 3145 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 5926 7381 618
Size: 325 217 627 773 3366 1455 1842
Fragments arranged by size:
3366 1842 1455 773 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5924 1391
Size: 4533 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2659 2825 2885 5778 928
Size: 971 760 166 60 2893 3755
Fragments arranged by size:
3755 2893 971 760 166 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2466 2500 6796 6830 1903
Size: 34 529 34 4296 34 3678
Fragments arranged by size:
4296 3678 529 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6497 7707 6497
Size: 1210 7395
BclI T'GATC_A
Cuts at: 1548 2287 4366 1548
Size: 739 2079 5787
Fragments arranged by size:
5787 2079 739
BfrBI ATG'CAT
Cuts at: 4212 4212
Size: 8605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7216 136
Size: 107 122 71 631 605 5544 1525
Fragments arranged by size:
5544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8600 8600
Size: 8605
BlpI GC'TnA_GC
Cuts at: 3441 4356 6022 3441
Size: 915 1666 6024
Fragments arranged by size:
6024 1666 915
Bme1580I G_kGCm'C
Cuts at: 1790 6666 7822 1790
Size: 4876 1156 2573
Fragments arranged by size:
4876 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2438 6336 7294
Size: 416 53 83 399 24 1021 3898 958
Cuts at: 7294 442
Size: 1753
Fragments arranged by size:
3898 1753 1021 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2262
Size: 8605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 5723 5757 7265 1239
Size: 3236 1248 34 1508 2579
Fragments arranged by size:
3236 2579 1508 1248 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2713 4817 6730 7595 7893 2713
Size: 2104 1913 865 298 3425
Fragments arranged by size:
3425 2104 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 7268 882
Size: 617 3540 2229 2219
Fragments arranged by size:
3540 2229 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6396 493
Size: 711 5192 2702
Fragments arranged by size:
5192 2702 711
BsaBI GATnn'nnATC
Cuts at: 8599 8599
Size: 8605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6412 6794 7429 275
Size: 53 83 186 936 4879 382 635 1451
Fragments arranged by size:
4879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7038 7436 7710 7857 8268 7038
Size: 398 274 147 411 7375
Fragments arranged by size:
7375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5789 5819 1178
Size: 30 165 30 4386 30 3964
Fragments arranged by size:
4386 3964 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4307 5270 1186
Size: 3121 963 4521
Fragments arranged by size:
4521 3121 963
BseYI C'CCAG_C
Cuts at: 7808 8304 7808
Size: 496 8109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3977 3981 1163
Size: 573 2241 4 5787
Fragments arranged by size:
5787 2241 573 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6055 6816 7844 1540
Size: 275 186 4054 761 1028 2301
Fragments arranged by size:
4054 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5277 5889 6666 6751 7822 5277
Size: 612 777 85 1071 6060
Fragments arranged by size:
6060 1071 777 612 85
BsmI GAATG_Cn'
Cuts at: 3442 4212 4306 6116 6209 3442
Size: 770 94 1810 93 5838
Fragments arranged by size:
5838 1810 770 94 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5277 5889 5924 6666 6751 7822 1391
Size: 399 3487 612 35 742 85 1071 2174
Fragments arranged by size:
3487 2174 1071 742 612 399 85 35
BspEI T'CCGG_A
Cuts at: 8268 8268
Size: 8605
BspHI T'CATG_A
Cuts at: 2088 4984 6494 8224 2088
Size: 2896 1510 1730 2469
Fragments arranged by size:
2896 2469 1730 1510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2377 3703 5960 844
Size: 1533 1326 2257 3489
Fragments arranged by size:
3489 2257 1533 1326
BsrBI CCG'CTC
Cuts at: 1394 1823 6492 1394
Size: 429 4669 3507
Fragments arranged by size:
4669 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2582 3118 7100 7274 65
Size: 1183 1334 536 3982 174 1396
Fragments arranged by size:
3982 1396 1334 1183 536 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7249 7436 1700
Size: 110 5439 187 2869
Fragments arranged by size:
5439 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4900 96
Size: 4804 3801
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8605
BssSI C'ACGA_G
Cuts at: 6665 7677 6665
Size: 1012 7593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8605
BstBI TT'CG_AA
Cuts at: 7409 7409
Size: 8605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8605
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 8605
BstYI r'GATC_y
Cuts at: 1972 2790 4699 5935 6302 6687 6704 8156 8242
Size: 818 1909 1236 367 385 17 1452 86
Cuts at: 8242 8254 8600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1909 1452 1236 818 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3287 3287
Size: 8605
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7114
Fragments arranged by size:
7114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2541 487
Size: 683 56 1315 6551
Fragments arranged by size:
6551 1315 683 56
BtsI GCAGTG_nn'
Cuts at: 6121 6915 6935 6121
Size: 794 20 7791
Fragments arranged by size:
7791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2718 2753 529
Size: 35 2154 35 6381
Fragments arranged by size:
6381 2154 35 35
DraI TTT'AAA
Cuts at: 2257 2857 2961 4725 5909 2257
Size: 600 104 1764 1184 4953
Fragments arranged by size:
4953 1764 1184 600 104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7612 809
Size: 507 6296 1802
Fragments arranged by size:
6296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3573 6052 6941
Size: 54 1425 50 275 18 1743 2479 889
Cuts at: 6941 8570 8
Size: 1629 43
Fragments arranged by size:
2479 1743 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6052 1537
Size: 275 4240 4090
Fragments arranged by size:
4240 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2376 3515 3690 3900 4056 4284 4440
Size: 725 298 1139 175 210 156 228 156
Cuts at: 4440 4650 4777 4826 5521 6535 1353
Size: 210 127 49 695 1014 3423
Fragments arranged by size:
3423 1139 1014 725 695 298 228 210
210 175 156 156 127 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7192 7566 7712 387
Size: 1064 138 5603 374 146 1280
Fragments arranged by size:
5603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2493 2837 2865 2916 3783 3882 3972
Size: 393 861 344 28 51 867 99 90
Cuts at: 3972 4475 4583 5229 5454 5723 5757 5834 6668
Size: 503 108 646 225 269 34 77 834
Cuts at: 6668 7265 8052 8320 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 867 861 834 787 646 597 503
393 344 269 268 225 108 99 90
77 51 34 28
EcoO109I rG'GnC_Cy
Cuts at: 3970 3970
Size: 8605
EcoRI G'AATT_C
Cuts at: 5914 6292 5914
Size: 378 8227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2882 3929 4271 2882
Size: 1047 342 7216
Fragments arranged by size:
7216 1047 342
FspI TGC'GCA
Cuts at: 7111 7111
Size: 8605
HaeII r_GCGC'y
Cuts at: 1678 7752 1678
Size: 6074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3121 3153 3285 3317 5789
Size: 32 135 32 1549 32 132 32 2472
Cuts at: 5789 5821 7246 7278 7320 7352 1373
Size: 32 1425 32 42 32 2626
Fragments arranged by size:
2626 2472 1549 1425 135 132 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 5949 6195 669
Size: 988 281 1140 2871 246 3079
Fragments arranged by size:
3079 2871 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2141 2634 3608 748
Size: 1393 493 974 5745
Fragments arranged by size:
5745 1393 974 493
HpaI GTT'AAC
Cuts at: 3078 6195 3078
Size: 3117 5488
KpnI G_GTAC'C
Cuts at: 2564 5930 2564
Size: 3366 5239
MfeI C'AATT_G
Cuts at: 6204 6204
Size: 8605
MluI A'CGCG_T
Cuts at: 8481 8481
Size: 8605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 3621 5106 7719 7903 1305
Size: 948 1368 1485 2613 184 2007
Fragments arranged by size:
2613 2007 1485 1368 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3575 10
Size: 54 1425 343 1743 5040
Fragments arranged by size:
5040 1743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6563 6922 7081 518
Size: 648 5397 359 159 2042
Fragments arranged by size:
5397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2480 3530 4888 6021 6698 7846 8091 1606
Size: 874 1050 1358 1133 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 1133 1050 874 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7114
Fragments arranged by size:
7114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8605
NotI GC'GGCC_GC
Cuts at: 6052 6052
Size: 8605
NsiI A_TGCA'T
Cuts at: 4214 4214
Size: 8605
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 5963 7508 1132
Size: 97 1962 1545 1227 1545 2229
Fragments arranged by size:
2229 1962 1545 1545 1227 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8605
PciI A'CATG_T
Cuts at: 3187 4732 7504 3187
Size: 1545 2772 4288
Fragments arranged by size:
4288 2772 1545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8605
PmeI GTTT'AAAC
Cuts at: 5909 5909
Size: 8605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6674 6706 8187 8219 6674
Size: 32 1481 32 7060
Fragments arranged by size:
7060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2730 1613
Size: 1117 7488
PsiI TTA'TAA
Cuts at: 3122 6175 3122
Size: 3053 5552
PstI C_TGCA'G
Cuts at: 830 3343 5957 830
Size: 2513 2614 3478
Fragments arranged by size:
3478 2614 2513
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8605
PvuII CAG'CTG
Cuts at: 2480 4888 2480
Size: 2408 6197
SalI G'TCGA_C
Cuts at: 1936 5947 1936
Size: 4011 4594
SapI GCTCTTCn'nnn_
Cuts at: 2376 4056 4440 2376
Size: 1680 384 6541
Fragments arranged by size:
6541 1680 384
SbfI CC_TGCA'GG
Cuts at: 5957 5957
Size: 8605
ScaI AGT'ACT
Cuts at: 6853 8565 6853
Size: 1712 6893
SexAI A'CCwGG_T
Cuts at: 5892 5892
Size: 8605
SfcI C'TryA_G
Cuts at: 826 1045 3339 3410 4154 4440 5375 5953 7088
Size: 219 2294 71 744 286 935 578 1135
Cuts at: 7088 7769 7960 826
Size: 681 191 1471
Fragments arranged by size:
2294 1471 1135 935 744 681 578 286
219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8605
SmaI CCC'GGG
Cuts at: 1872 5932 1872
Size: 4060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2247 2728 3914 4400 4796 6709
Size: 197 926 304 481 1186 486 396 1913
Cuts at: 6709 7610 7872 820
Size: 901 262 1553
Fragments arranged by size:
1913 1553 1186 926 901 486 481 396
304 262 197
SnaBI TAC'GTA
Cuts at: 493 6396 493
Size: 5903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8605
SphI G_CATG'C
Cuts at: 1132 5963 1132
Size: 4831 3774
SspI AAT'ATT
Cuts at: 5 52 6529 5
Size: 47 6477 2081
Fragments arranged by size:
6477 2081 47
StuI AGG'CCT
Cuts at: 1517 4769 1517
Size: 3252 5353
StyI C'CwwG_G
Cuts at: 513 1065 2004 3621 6044 513
Size: 552 939 1617 2423 3074
Fragments arranged by size:
3074 2423 1617 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2837 6632 6649 6802 6987 7432 325
Size: 1255 1257 3795 17 153 185 445 1498
Fragments arranged by size:
3795 1498 1257 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3589 4900 6851
Size: 274 80 33 51 1443 1612 1311 1951
Cuts at: 6851 8563 96
Size: 1712 138
Fragments arranged by size:
1951 1712 1612 1443 1311 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8565 8597 8565
Size: 32 8573
Tth111I GACn'n_nGTC
Cuts at: 1316 5803 1316
Size: 4487 4118
XbaI T'CTAG_A
Cuts at: 5269 5941 5269
Size: 672 7933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4421 4421
Size: 8605
XhoI C'TCGA_G
Cuts at: 1943 2247 1943
Size: 304 8301
XmaI C'CCGG_G
Cuts at: 1870 5930 1870
Size: 4060 4545
XmnI GAAnn'nnTTC
Cuts at: 3501 4449 6734 3501
Size: 948 2285 5372
Fragments arranged by size:
5372 2285 948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6413 276
Size: 53 83 186 936 4879 2468
Fragments arranged by size:
4879 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI