Restriction map for
pFN21AE1029
(Circular) (Six-base) MAPSORT of: pFN21AE1029.seq Check: 9397 from: 1 to: 8524
pFN21AE1029 8524 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2752 5050 2752
Size: 2298 6226
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6334 278
Size: 53 83 186 936 4798 2468
Fragments arranged by size:
4798 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 4650 5620 5867 1937
Size: 496 2217 970 247 4594
Fragments arranged by size:
4594 2217 970 496 247
Acc65I G'GTAC_C
Cuts at: 5845 5845
Size: 8524
AclI AA'CG_TT
Cuts at: 1563 6651 7024 1563
Size: 5088 373 3063
Fragments arranged by size:
5088 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 2679 3365 3960 6587 7971 8239 1632
Size: 755 292 686 595 2627 1384 268 1917
Fragments arranged by size:
2627 1917 1384 755 686 595 292 268
AfeI AGC'GCT
Cuts at: 5294 5294
Size: 8524
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8327
AflIII A'CryG_T
Cuts at: 1203 7423 8400 1203
Size: 6220 977 1327
Fragments arranged by size:
6220 1327 977
AgeI A'CCGG_T
Cuts at: 2035 7355 2035
Size: 5320 3204
AhdI GACnn_n'nnGTC
Cuts at: 2029 7253 2029
Size: 5224 3300
AleI CACnn'nnGTG
Cuts at: 1166 2858 4569 1166
Size: 1692 1711 5121
Fragments arranged by size:
5121 1711 1692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3042 1184
Size: 1858 6666
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3295 3327 3295
Size: 32 8492
AlwNI CAG_nnn'CTG
Cuts at: 2138 2365 2717 6267 7839 2138
Size: 227 352 3550 1572 2823
Fragments arranged by size:
3550 2823 1572 352 227
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 8524
ApaLI G'TGCA_C
Cuts at: 6581 7737 6581
Size: 1156 7368
ApoI r'AATT_y
Cuts at: 1493 2163 2462 3448 3533 3953 5266 5323 5518
Size: 670 299 986 85 420 1313 57 195
Cuts at: 5518 5833 6059 6211 8320 1493
Size: 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 986 670 420 315 299
226 195 152 85 57
AseI AT'TA_AT
Cuts at: 160 1032 7078 160
Size: 872 6046 1606
Fragments arranged by size:
6046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8524
AvaI C'yCGr_G
Cuts at: 1870 1943 5849 1870
Size: 73 3906 4545
Fragments arranged by size:
4545 3906 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3744 3777 3744
Size: 33 8491
BamHI G'GATC_C
Cuts at: 2061 5400 5854 6221 2061
Size: 3339 454 367 4364
Fragments arranged by size:
4364 3339 454 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5731 5845 7300 618
Size: 325 217 627 3944 114 1455 1842
Fragments arranged by size:
3944 1842 1455 627 325 217 114
BanII G_rGCy'C
Cuts at: 1391 2216 2232 3671 5590 5843 1391
Size: 825 16 1439 1919 253 4072
Fragments arranged by size:
4072 1919 1439 825 253 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3815 4356 5471 928
Size: 971 1916 541 1115 3981
Fragments arranged by size:
3981 1916 1115 971 541
BbvCI CC'TCA_GC
Cuts at: 2733 2733
Size: 8524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6715 6749 1903
Size: 34 4778 34 3678
Fragments arranged by size:
4778 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6416 7626 6416
Size: 1210 7314
BclI T'GATC_A
Cuts at: 1548 3211 5541 1548
Size: 1663 2330 4531
Fragments arranged by size:
4531 2330 1663
BfrBI ATG'CAT
Cuts at: 2822 4453 2822
Size: 1631 6893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3259 3273 4550
Size: 107 122 71 631 605 1587 14 1277
Cuts at: 4550 7135 136
Size: 2585 1525
Fragments arranged by size:
2585 1587 1525 1277 631 605 122 107
71 14
BglII A'GATC_T
Cuts at: 5049 8519 5049
Size: 3470 5054
BlpI GC'TnA_GC
Cuts at: 3498 5166 5941 3498
Size: 1668 775 6081
Fragments arranged by size:
6081 1668 775
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3983 5673 5736 6585 7741 1790
Size: 442 1751 1690 63 849 1156 2573
Fragments arranged by size:
2573 1751 1690 1156 849 442 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2695 6255 7213
Size: 416 53 83 399 24 1278 3560 958
Cuts at: 7213 442
Size: 1753
Fragments arranged by size:
3560 1753 1278 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2328 3505 4511 2328
Size: 1177 1006 6341
Fragments arranged by size:
6341 1177 1006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 3039 3060 3297 3655 3995 5162 5519
Size: 1772 28 21 237 358 340 1167 357
Cuts at: 5519 5741 7184 1239
Size: 222 1443 2579
Fragments arranged by size:
2579 1772 1443 1167 358 357 340 237
222 28 21
Bpu10I CC'TnA_GC
Cuts at: 2733 5343 2733
Size: 2610 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5113 6649 7514 7812 5113
Size: 1536 865 298 5825
Fragments arranged by size:
5825 1536 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 3869 5684 5753 7187 882
Size: 617 636 1734 1815 69 1434 2219
Fragments arranged by size:
2219 1815 1734 1434 636 617 69
BsaAI yAC'GTr
Cuts at: 493 1204 5308 6315 493
Size: 711 4104 1007 2702
Fragments arranged by size:
4104 2702 1007 711
BsaBI GATnn'nnATC
Cuts at: 8518 8518
Size: 8524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6331 6713 7348 275
Size: 53 83 186 936 4798 382 635 1451
Fragments arranged by size:
4798 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2035 5102 5403 6957 7355 7629 7776 8187 2035
Size: 3067 301 1554 398 274 147 411 2372
Fragments arranged by size:
3067 2372 1554 411 398 301 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2280 2310 3210 3240 3295
Size: 30 165 30 877 30 900 30 55
Cuts at: 3295 3325 5135 5165 1178
Size: 30 1810 30 4537
Fragments arranged by size:
4537 1810 900 877 165 55 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2721 3717 4316 5276 1186
Size: 1535 996 599 960 4434
Fragments arranged by size:
4434 1535 996 960 599
BseYI C'CCAG_C
Cuts at: 2049 2183 2511 2688 2710 2721 3263 4795 4882
Size: 134 328 177 22 11 542 1532 87
Cuts at: 4882 7727 8223 2049
Size: 2845 496 2350
Fragments arranged by size:
2845 2350 1532 542 496 328 177 134
87 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2316 2360 3453 4682 5078 1163
Size: 573 561 19 44 1093 1229 396 4609
Fragments arranged by size:
4609 1229 1093 573 561 396 44 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5974 6735 7763 1540
Size: 275 186 3973 761 1028 2301
Fragments arranged by size:
3973 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 6585 6670 7741 2216
Size: 4369 85 1071 2999
Fragments arranged by size:
4369 2999 1071 85
BsmI GAATG_Cn'
Cuts at: 2984 4312 4413 6035 6128 2984
Size: 1328 101 1622 93 5380
Fragments arranged by size:
5380 1622 1328 101 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3101 4847 3101
Size: 1746 6778
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2232 3671 3983 5590 5673 5736
Size: 399 426 16 1439 312 1607 83 63
Cuts at: 5736 5843 6585 6670 7741 1391
Size: 107 742 85 1071 2174
Fragments arranged by size:
2174 1607 1439 1071 742 426 399 312
107 85 83 63 16
BspEI T'CCGG_A
Cuts at: 5403 8187 5403
Size: 2784 5740
BspHI T'CATG_A
Cuts at: 2454 6413 8143 2454
Size: 3959 1730 2835
Fragments arranged by size:
3959 2835 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2752 5050 5879 844
Size: 1908 2298 829 3489
Fragments arranged by size:
3489 2298 1908 829
BsrBI CCG'CTC
Cuts at: 1394 1823 6411 1394
Size: 429 4588 3507
Fragments arranged by size:
4588 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3627 4071 4980 7019 7193 65
Size: 1183 2379 444 909 2039 174 1396
Fragments arranged by size:
2379 2039 1396 1183 909 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2839 7168 7355 1700
Size: 110 225 804 4329 187 2869
Fragments arranged by size:
4329 2869 804 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 3586 3713 96
Size: 3490 127 4907
Fragments arranged by size:
4907 3490 127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8524
BssSI C'ACGA_G
Cuts at: 3492 6584 7596 3492
Size: 3092 1012 4420
Fragments arranged by size:
4420 3092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 794
Size: 1307 7217
BstBI TT'CG_AA
Cuts at: 7328 7328
Size: 8524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8524
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 8524
BstYI r'GATC_y
Cuts at: 1972 2061 3282 5049 5400 5854 6221 6606 6623
Size: 89 1221 1767 351 454 367 385 17
Cuts at: 6623 8075 8161 8173 8519 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1767 1452 1221 454 385 367 351
346 89 86 17 12
BstZ17I GTA'TAC
Cuts at: 4651 4651
Size: 8524
Bsu36I CC'TnA_GG
Cuts at: 2427 4235 4715 2427
Size: 1808 480 6236
Fragments arranged by size:
6236 1808 480
BtgI C'CryG_G
Cuts at: 513 1065 2284 2644 4992 5469 513
Size: 552 1219 360 2348 477 3568
Fragments arranged by size:
3568 2348 1219 552 477 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7785
Fragments arranged by size:
7785 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 6040 6834 6854 2139
Size: 3901 794 20 3809
Fragments arranged by size:
3901 3809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3361 3396 529
Size: 35 2797 35 5657
Fragments arranged by size:
5657 2797 35 35
DraI TTT'AAA
Cuts at: 2467 3103 3559 3747 4213 5828 2467
Size: 636 456 188 466 1615 5163
Fragments arranged by size:
5163 1615 636 466 456 188
DraIII CAC_nnn'GTG
Cuts at: 2101 4396 2101
Size: 2295 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2149 4229 7531 809
Size: 507 833 2080 3302 1802
Fragments arranged by size:
3302 2080 1802 833 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2620 2787 5971
Size: 54 1425 50 275 18 790 167 3184
Cuts at: 5971 6860 8489 8
Size: 889 1629 43
Fragments arranged by size:
3184 1629 1425 889 790 275 167 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5971 1537
Size: 275 4159 4090
Fragments arranged by size:
4159 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3429 4394 4506 4518 4664 5253 6454 1353
Size: 2076 965 112 12 146 589 1201 3423
Fragments arranged by size:
3423 2076 1201 965 589 146 112 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2826 5413 7111 7485 7631 387
Size: 1064 138 1237 2587 1698 374 146 1280
Fragments arranged by size:
2587 1698 1280 1237 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2214 2214
Size: 8524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2679 3011 3039 3060 3297 3365
Size: 393 755 292 332 28 21 237 68
Cuts at: 3365 3655 3960 3995 5162 5519 5741 6587 7184
Size: 290 305 35 1167 357 222 846 597
Cuts at: 7184 7971 8239 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1167 846 787 755 597 393 357
332 305 292 290 268 237 222 68
35 28 21
EcoO109I rG'GnC_Cy
Cuts at: 4231 5725 4231
Size: 1494 7030
EcoRI G'AATT_C
Cuts at: 2163 5266 5323 5518 5833 6211 2163
Size: 3103 57 195 315 378 4476
Fragments arranged by size:
4476 3103 378 315 195 57
FspI TGC'GCA
Cuts at: 7030 7030
Size: 8524
HaeII r_GCGC'y
Cuts at: 1678 5296 7671 1678
Size: 3618 2375 2531
Fragments arranged by size:
3618 2531 2375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2369 2401 3107
Size: 32 135 32 443 32 322 32 706
Cuts at: 3107 3139 3196 3210 3228 3242 4048 4080 5282
Size: 32 57 14 18 14 806 32 1202
Cuts at: 5282 5314 5321 5353 7165 7197 7239 7271 1373
Size: 32 7 32 1812 32 42 32 2626
Fragments arranged by size:
2626 1812 1202 806 706 443 322 135
57 42 32 32 32 32 32 32
32 32 32 32 18 14 14 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4669 5148 5414 5868 6114 669
Size: 988 281 2731 479 266 454 246 3079
Fragments arranged by size:
3079 2731 988 479 454 281 266 246
HindIII A'AGCT_T
Cuts at: 748 3920 5198 748
Size: 3172 1278 4074
Fragments arranged by size:
4074 3172 1278
HpaI GTT'AAC
Cuts at: 6114 6114
Size: 8524
KpnI G_GTAC'C
Cuts at: 5849 5849
Size: 8524
MfeI C'AATT_G
Cuts at: 4083 6123 4083
Size: 2040 6484
MluI A'CGCG_T
Cuts at: 8400 8400
Size: 8524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2039 7638 7822 1305
Size: 734 5599 184 2007
Fragments arranged by size:
5599 2007 734 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2622 10
Size: 54 1425 343 790 5912
Fragments arranged by size:
5912 1425 790 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 3381 3632 4569 5604 6482 6841
Size: 648 1692 523 251 937 1035 878 359
Cuts at: 6841 7000 518
Size: 159 2042
Fragments arranged by size:
2042 1692 1035 937 878 648 523 359
251 159
MspA1I CmG'CkG
Cuts at: 1606 2273 2330 4037 5026 5113 5158 5233 5940
Size: 667 57 1707 989 87 45 75 707
Cuts at: 5940 6617 7765 8010 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1707 1148 989 707 677 667 245
87 75 57 45
NcoI C'CATG_G
Cuts at: 513 1065 2644 4992 5469 513
Size: 552 1579 2348 477 3568
Fragments arranged by size:
3568 2348 1579 552 477
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8524
NotI GC'GGCC_GC
Cuts at: 5971 5971
Size: 8524
NsiI A_TGCA'T
Cuts at: 2824 4455 2824
Size: 1631 6893
NspI r_CATG'y
Cuts at: 1132 1229 5669 5882 7427 1132
Size: 97 4440 213 1545 2229
Fragments arranged by size:
4440 2229 1545 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8524
PciI A'CATG_T
Cuts at: 7423 7423
Size: 8524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5700 1108
Size: 4592 3932
PfoI T'CCnGG_A
Cuts at: 2988 4890 5139 2988
Size: 1902 249 6373
Fragments arranged by size:
6373 1902 249
PmeI GTTT'AAAC
Cuts at: 5828 5828
Size: 8524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3327 6593 6625 8106 8138 3295
Size: 32 3266 32 1481 32 3681
Fragments arranged by size:
3681 3266 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4231 4231
Size: 8524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8524
PsiI TTA'TAA
Cuts at: 6094 6094
Size: 8524
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 8524
PstI C_TGCA'G
Cuts at: 830 2412 5031 5876 830
Size: 1582 2619 845 3478
Fragments arranged by size:
3478 2619 1582 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8524
PvuII CAG'CTG
Cuts at: 2330 4037 5026 5113 5233 2330
Size: 1707 989 87 120 5621
Fragments arranged by size:
5621 1707 989 120 87
SacI G_AGCT'C
Cuts at: 2216 2216
Size: 8524
SalI G'TCGA_C
Cuts at: 1936 5866 1936
Size: 3930 4594
SapI GCTCTTCn'nnn_
Cuts at: 3429 3429
Size: 8524
SbfI CC_TGCA'GG
Cuts at: 5876 5876
Size: 8524
ScaI AGT'ACT
Cuts at: 2171 6772 8484 2171
Size: 4601 1712 2211
Fragments arranged by size:
4601 2211 1712
SexAI A'CCwGG_T
Cuts at: 2190 2397 2190
Size: 207 8317
SfcI C'TryA_G
Cuts at: 826 1045 2235 2408 3297 5027 5872 7007 7688
Size: 219 1190 173 889 1730 845 1135 681
Cuts at: 7688 7879 826
Size: 191 1471
Fragments arranged by size:
1730 1471 1190 1135 889 845 681 219
191 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8524
SgrAI Cr'CCGG_yG
Cuts at: 1700 2035 1700
Size: 335 8189
SmaI CCC'GGG
Cuts at: 1872 5851 1872
Size: 3979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5128 6628 7529 7791 820
Size: 197 926 3185 1500 901 262 1553
Fragments arranged by size:
3185 1553 1500 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6315 493
Size: 5822 2702
SpeI A'CTAG_T
Cuts at: 152 3511 152
Size: 3359 5165
SphI G_CATG'C
Cuts at: 1132 5882 1132
Size: 4750 3774
SspI AAT'ATT
Cuts at: 5 52 6448 5
Size: 47 6396 2081
Fragments arranged by size:
6396 2081 47
StuI AGG'CCT
Cuts at: 1517 5639 1517
Size: 4122 4402
StyI C'CwwG_G
Cuts at: 513 1065 2644 3030 3973 4227 4992 5218 5469
Size: 552 1579 386 943 254 765 226 251
Cuts at: 5469 5658 5963 513
Size: 189 305 3074
Fragments arranged by size:
3074 1579 943 765 552 386 305 254
251 226 189
SwaI ATTT'AAAT
Cuts at: 2467 2467
Size: 8524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 2280 2657 5699 6551 6568 6721
Size: 1255 617 83 377 3042 852 17 153
Cuts at: 6721 6906 7351 325
Size: 185 445 1498
Fragments arranged by size:
3042 1498 1255 852 617 445 377 185
153 83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 3586 3713
Size: 274 80 33 51 1443 192 1417 127
Cuts at: 3713 6770 8482 96
Size: 3057 1712 138
Fragments arranged by size:
3057 1712 1443 1417 274 192 138 127
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 8484 8516 2280
Size: 32 6172 32 2288
Fragments arranged by size:
6172 2288 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8524
XbaI T'CTAG_A
Cuts at: 5860 5860
Size: 8524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3958 5476 3958
Size: 1518 7006
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8524
XmaI C'CCGG_G
Cuts at: 1870 5849 1870
Size: 3979 4545
XmnI GAAnn'nnTTC
Cuts at: 6653 6653
Size: 8524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6332 276
Size: 53 83 186 936 4798 2468
Fragments arranged by size:
4798 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BsiWI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI