Restriction map for
pFN21AE1028
(Circular) (Six-base) MAPSORT of: pFN21AE1028.seq Check: 3099 from: 1 to: 8356
pFN21AE1028 8356 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2008 2008
Size: 8356
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6166 278
Size: 53 83 186 936 4630 2468
Fragments arranged by size:
4630 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4319 5699 1937
Size: 2382 1380 4594
Fragments arranged by size:
4594 2382 1380
Acc65I G'GTAC_C
Cuts at: 5677 5677
Size: 8356
AclI AA'CG_TT
Cuts at: 1563 2977 6483 6856 1563
Size: 1414 3506 373 3063
Fragments arranged by size:
3506 3063 1414 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 3294 4409 4679 5107 6419 7803 8071
Size: 424 1238 1115 270 428 1312 1384 268
Cuts at: 8071 1632
Size: 1917
Fragments arranged by size:
1917 1384 1312 1238 1115 428 424 270
268
AfeI AGC'GCT
Cuts at: 2386 4712 2386
Size: 2326 6030
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8159
AflIII A'CryG_T
Cuts at: 1203 3877 7255 8232 1203
Size: 2674 3378 977 1327
Fragments arranged by size:
3378 2674 1327 977
AgeI A'CCGG_T
Cuts at: 2265 7187 2265
Size: 4922 3434
AhdI GACnn_n'nnGTC
Cuts at: 7085 7085
Size: 8356
AleI CACnn'nnGTG
Cuts at: 1166 3650 1166
Size: 2484 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3468 1184
Size: 2284 6072
AlwNI CAG_nnn'CTG
Cuts at: 3001 4679 4882 5449 6099 7671 3001
Size: 1678 203 567 650 1572 3686
Fragments arranged by size:
3686 1678 1572 650 567 203
ApaLI G'TGCA_C
Cuts at: 6413 7569 6413
Size: 1156 7200
ApoI r'AATT_y
Cuts at: 1493 2844 3053 4184 4830 4978 5558 5665 5891
Size: 1351 209 1131 646 148 580 107 226
Cuts at: 5891 6043 8152 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1351 1131 646 580 226 209
152 148 107
AseI AT'TA_AT
Cuts at: 160 1032 3222 4125 6910 160
Size: 872 2190 903 2785 1606
Fragments arranged by size:
2785 2190 1606 903 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8356
AvaI C'yCGr_G
Cuts at: 1870 1943 3342 4482 5681 1870
Size: 73 1399 1140 1199 4545
Fragments arranged by size:
4545 1399 1199 1140 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2487 2520 2487
Size: 33 8323
BamHI G'GATC_C
Cuts at: 5686 6053 5686
Size: 367 7989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 5677 7132 618
Size: 325 217 627 525 3365 1455 1842
Fragments arranged by size:
3365 1842 1455 627 525 325 217
BanII G_rGCy'C
Cuts at: 1391 2671 5675 1391
Size: 1280 3004 4072
Fragments arranged by size:
4072 3004 1280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3273 928
Size: 971 1374 6011
Fragments arranged by size:
6011 1374 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2693 2727 6547 6581 1903
Size: 34 756 34 3820 34 3678
Fragments arranged by size:
3820 3678 756 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3787 6248 7458 3787
Size: 2461 1210 4685
Fragments arranged by size:
4685 2461 1210
BclI T'GATC_A
Cuts at: 1548 3831 4138 5604 1548
Size: 2283 307 1466 4300
Fragments arranged by size:
4300 2283 1466 307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6967 136
Size: 107 122 71 631 605 5295 1525
Fragments arranged by size:
5295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3469 8351 3469
Size: 4882 3474
BlpI GC'TnA_GC
Cuts at: 2736 2781 5457 5773 2736
Size: 45 2676 316 5319
Fragments arranged by size:
5319 2676 316 45
Bme1580I G_kGCm'C
Cuts at: 1790 6417 7573 1790
Size: 4627 1156 2573
Fragments arranged by size:
4627 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4008 4355 6087
Size: 416 53 83 399 24 2591 347 1732
Cuts at: 6087 7045 442
Size: 958 1753
Fragments arranged by size:
2591 1753 1732 958 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3317 3771 5307 7016 1239
Size: 2078 454 1536 1709 2579
Fragments arranged by size:
2579 2078 1709 1536 454
Bpu10I CC'TnA_GC
Cuts at: 4269 4269
Size: 8356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3087 3185 4931 5190 5564 6481 7346 7644 3087
Size: 98 1746 259 374 917 865 298 3799
Fragments arranged by size:
3799 1746 917 865 374 298 259 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 7019 882
Size: 617 2664 2856 2219
Fragments arranged by size:
2856 2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6147 493
Size: 711 4943 2702
Fragments arranged by size:
4943 2702 711
BsaBI GATnn'nnATC
Cuts at: 8350 8350
Size: 8356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6163 6545 7180 275
Size: 53 83 186 936 4630 382 635 1451
Fragments arranged by size:
4630 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2265 6789 7187 7461 7608 8019 2265
Size: 4524 398 274 147 411 2602
Fragments arranged by size:
4524 2602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8131
Fragments arranged by size:
8131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8356
BseYI C'CCAG_C
Cuts at: 3924 4173 7559 8055 3924
Size: 249 3386 496 4225
Fragments arranged by size:
4225 3386 496 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7783
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5806 6567 7595 1540
Size: 275 186 3805 761 1028 2301
Fragments arranged by size:
3805 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3994 6417 6502 7573 3994
Size: 2423 85 1071 4777
Fragments arranged by size:
4777 2423 1071 85
BsmI GAATG_Cn'
Cuts at: 3439 5867 5960 3439
Size: 2428 93 5835
Fragments arranged by size:
5835 2428 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2656 2656
Size: 8356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 3994 5675 6417 6502 7573 1391
Size: 399 881 1323 1681 742 85 1071 2174
Fragments arranged by size:
2174 1681 1323 1071 881 742 399 85
BspEI T'CCGG_A
Cuts at: 8019 8019
Size: 8356
BspHI T'CATG_A
Cuts at: 4753 6245 7975 4753
Size: 1492 1730 5134
Fragments arranged by size:
5134 1730 1492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 4110 5711 844
Size: 1164 2102 1601 3489
Fragments arranged by size:
3489 2102 1601 1164
BsrBI CCG'CTC
Cuts at: 1394 1823 6243 1394
Size: 429 4420 3507
Fragments arranged by size:
4420 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6851 7025 65
Size: 1183 5603 174 1396
Fragments arranged by size:
5603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 7000 7187 1700
Size: 110 455 4735 187 2869
Fragments arranged by size:
4735 2869 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 5500 96
Size: 1911 3493 2952
Fragments arranged by size:
3493 2952 1911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8356
BssSI C'ACGA_G
Cuts at: 2217 2709 6416 7428 2217
Size: 492 3707 1012 3145
Fragments arranged by size:
3707 3145 1012 492
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3001 3455 4795 794
Size: 2207 454 1340 4355
Fragments arranged by size:
4355 2207 1340 454
BstBI TT'CG_AA
Cuts at: 2038 2299 4903 7160 2038
Size: 261 2604 2257 3234
Fragments arranged by size:
3234 2604 2257 261
BstEII G'GTnAC_C
Cuts at: 1184 2268 3968 1184
Size: 1084 1700 5572
Fragments arranged by size:
5572 1700 1084
BstXI CCAn_nnnn'nTGG
Cuts at: 5285 5285
Size: 8356
BstYI r'GATC_y
Cuts at: 1972 2065 2108 3124 3322 3469 5686 6053 6438
Size: 93 43 1016 198 147 2217 367 385
Cuts at: 6438 6455 7907 7993 8005 8351 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2217 1977 1452 1016 385 367 346 198
147 93 86 43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7804
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7617
Fragments arranged by size:
7617 683 56
BtsI GCAGTG_nn'
Cuts at: 3660 4922 5266 5872 6666 6686 3660
Size: 1262 344 606 794 20 5330
Fragments arranged by size:
5330 1262 794 606 344 20
ClaI AT'CG_AT
Cuts at: 1554 2277 5042 1554
Size: 723 2765 4868
Fragments arranged by size:
4868 2765 723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8321
DraI TTT'AAA
Cuts at: 3522 4189 5660 3522
Size: 667 1471 6218
Fragments arranged by size:
6218 1471 667
DraIII CAC_nnn'GTG
Cuts at: 5654 5654
Size: 8356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7363 809
Size: 507 6047 1802
Fragments arranged by size:
6047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5619 5803 6692
Size: 54 1425 50 275 18 3789 184 889
Cuts at: 6692 8321 8
Size: 1629 43
Fragments arranged by size:
3789 1629 1425 889 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5803 1537
Size: 275 3991 4090
Fragments arranged by size:
4090 3991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2296 2589 3423 4232 4382 4694 4809 5013
Size: 943 293 834 809 150 312 115 204
Cuts at: 5013 6286 1353
Size: 1273 3423
Fragments arranged by size:
3423 1273 943 834 809 312 293 204
150 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6943 7317 7463 387
Size: 1064 138 5354 374 146 1280
Fragments arranged by size:
5354 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 3294 3317 3771 4409 4679 5107
Size: 393 424 1238 23 454 638 270 428
Cuts at: 5107 5307 6419 7016 7803 8071 1239
Size: 200 1112 597 787 268 1524
Fragments arranged by size:
1524 1238 1112 787 638 597 454 428
424 393 270 268 200 23
EcoRI G'AATT_C
Cuts at: 5665 6043 5665
Size: 378 7978
EcoRV GAT'ATC
Cuts at: 2812 2812
Size: 8356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2312 2424 2891 2312
Size: 112 467 7777
Fragments arranged by size:
7777 467 112
FspI TGC'GCA
Cuts at: 4106 6862 4106
Size: 2756 5600
HaeII r_GCGC'y
Cuts at: 1678 2388 4714 7503 1678
Size: 710 2326 2789 2531
Fragments arranged by size:
2789 2531 2326 710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3835 3867 4768 4800 6997
Size: 32 135 32 2263 32 901 32 2197
Cuts at: 6997 7029 7071 7103 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2263 2197 901 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4421 4951 5097 5275 5700 5946
Size: 988 281 2483 530 146 178 425 246
Cuts at: 5946 669
Size: 3079
Fragments arranged by size:
3079 2483 988 530 425 281 246 178
146
HindIII A'AGCT_T
Cuts at: 748 3867 4300 748
Size: 3119 433 4804
Fragments arranged by size:
4804 3119 433
HpaI GTT'AAC
Cuts at: 4951 5097 5946 4951
Size: 146 849 7361
Fragments arranged by size:
7361 849 146
KpnI G_GTAC'C
Cuts at: 5681 5681
Size: 8356
MfeI C'AATT_G
Cuts at: 2058 5955 2058
Size: 3897 4459
MluI A'CGCG_T
Cuts at: 8232 8232
Size: 8356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2898 5272 7470 7654 1305
Size: 1593 2374 2198 184 2007
Fragments arranged by size:
2374 2198 2007 1593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5621 10
Size: 54 1425 343 3789 2745
Fragments arranged by size:
3789 2745 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3650 3987 4740 5598 6314 6673 6832
Size: 648 2484 337 753 858 716 359 159
Cuts at: 6832 518
Size: 2042
Fragments arranged by size:
2484 2042 858 753 716 648 359 337
159
MspA1I CmG'CkG
Cuts at: 1606 2185 3928 5552 5772 6449 7597 7842 1606
Size: 579 1743 1624 220 677 1148 245 2120
Fragments arranged by size:
2120 1743 1624 1148 677 579 245 220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7804
NdeI CA'TA_TG
Cuts at: 387 4737 387
Size: 4350 4006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8356
NotI GC'GGCC_GC
Cuts at: 5803 5803
Size: 8356
NspI r_CATG'y
Cuts at: 1132 1229 3587 3651 3881 4310 4798 5507 5714
Size: 97 2358 64 230 429 488 709 207
Cuts at: 5714 7259 1132
Size: 1545 2229
Fragments arranged by size:
2358 2229 1545 709 488 429 230 207
97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8356
PciI A'CATG_T
Cuts at: 3877 7255 3877
Size: 3378 4978
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5449 1108
Size: 4341 4015
PfoI T'CCnGG_A
Cuts at: 3748 3748
Size: 8356
PmeI GTTT'AAAC
Cuts at: 5660 5660
Size: 8356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6425 6457 7938 7970 6425
Size: 32 1481 32 6811
Fragments arranged by size:
6811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8356
PsiI TTA'TAA
Cuts at: 5926 5926
Size: 8356
PspXI vC'TCGA_Gb
Cuts at: 3342 4482 3342
Size: 1140 7216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 8324
PstI C_TGCA'G
Cuts at: 830 5708 830
Size: 4878 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8356
PvuII CAG'CTG
Cuts at: 3928 5552 3928
Size: 1624 6732
SalI G'TCGA_C
Cuts at: 1936 5698 1936
Size: 3762 4594
SbfI CC_TGCA'GG
Cuts at: 5708 5708
Size: 8356
ScaI AGT'ACT
Cuts at: 6604 8316 6604
Size: 1712 6644
SexAI A'CCwGG_T
Cuts at: 5149 5149
Size: 8356
SfcI C'TryA_G
Cuts at: 826 1045 5704 6839 7520 7711 826
Size: 219 4659 1135 681 191 1471
Fragments arranged by size:
4659 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8356
SmaI CCC'GGG
Cuts at: 1872 5683 1872
Size: 3811 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3102 3200 3342 4482 4910 5205
Size: 197 926 1159 98 142 1140 428 295
Cuts at: 5205 5543 6460 7361 7623 820
Size: 338 917 901 262 1553
Fragments arranged by size:
1553 1159 1140 926 917 901 428 338
295 262 197 142 98
SnaBI TAC'GTA
Cuts at: 493 6147 493
Size: 5654 2702
SpeI A'CTAG_T
Cuts at: 152 2747 152
Size: 2595 5761
SphI G_CATG'C
Cuts at: 1132 4310 4798 5714 1132
Size: 3178 488 916 3774
Fragments arranged by size:
3774 3178 916 488
SspI AAT'ATT
Cuts at: 5 52 3368 4114 6280 5
Size: 47 3316 746 2166 2081
Fragments arranged by size:
3316 2166 2081 746 47
StuI AGG'CCT
Cuts at: 1517 5533 1517
Size: 4016 4340
StyI C'CwwG_G
Cuts at: 513 1065 2931 5367 5795 513
Size: 552 1866 2436 428 3074
Fragments arranged by size:
3074 2436 1866 552 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6383 6400 6553 6738 7183 325
Size: 1255 4803 17 153 185 445 1498
Fragments arranged by size:
4803 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4938 5062
Size: 274 80 33 51 1443 30 2931 124
Cuts at: 5062 5500 6602 8314 96
Size: 438 1102 1712 138
Fragments arranged by size:
2931 1712 1443 1102 438 274 138 124
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5286 5318 8316 8348 5286
Size: 32 2998 32 5294
Fragments arranged by size:
5294 2998 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3974 1316
Size: 2658 5698
XbaI T'CTAG_A
Cuts at: 2611 4196 5692 2611
Size: 1585 1496 5275
Fragments arranged by size:
5275 1585 1496
XhoI C'TCGA_G
Cuts at: 1943 3342 4482 1943
Size: 1399 1140 5817
Fragments arranged by size:
5817 1399 1140
XmaI C'CCGG_G
Cuts at: 1870 5681 1870
Size: 3811 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 5088 6485 4259
Size: 829 1397 6130
Fragments arranged by size:
6130 1397 829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6164 276
Size: 53 83 186 936 4630 2468
Fragments arranged by size:
4630 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI