Restriction map for
pFN21AE1027
(Circular) (Six-base) MAPSORT of: pFN21AE1027.seq Check: 2764 from: 1 to: 7810
pFN21AE1027 7810 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2835 3047 3294 2835
Size: 212 247 7351
Fragments arranged by size:
7351 247 212
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5620 278
Size: 53 83 186 936 4084 2468
Fragments arranged by size:
4084 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5153 1937
Size: 3216 4594
Acc65I G'GTAC_C
Cuts at: 5131 5131
Size: 7810
AclI AA'CG_TT
Cuts at: 1563 5937 6310 1563
Size: 4374 373 3063
Fragments arranged by size:
4374 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2868 3677 4241 4595 4691 5873 7257 7525
Size: 1236 809 564 354 96 1182 1384 268
Cuts at: 7525 1632
Size: 1917
Fragments arranged by size:
1917 1384 1236 1182 809 564 354 268
96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7613
AflIII A'CryG_T
Cuts at: 1203 6709 7686 1203
Size: 5506 977 1327
Fragments arranged by size:
5506 1327 977
AgeI A'CCGG_T
Cuts at: 6641 6641
Size: 7810
AhdI GACnn_n'nnGTC
Cuts at: 4020 6539 4020
Size: 2519 5291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4214 1184
Size: 3030 4780
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2585 2617 3332 3364 2180
Size: 32 373 32 715 32 6626
Fragments arranged by size:
6626 715 373 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2233 2989 3376 3565 3673 3700 3889 4309 4732
Size: 756 387 189 108 27 189 420 423
Cuts at: 4732 5553 7125 2233
Size: 821 1572 2918
Fragments arranged by size:
2918 1572 821 756 423 420 387 189
189 108 27
ApaI G_GGCC'C
Cuts at: 2050 2661 2960 3134 3615 4396 2050
Size: 611 299 174 481 781 5464
Fragments arranged by size:
5464 781 611 481 299 174
ApaLI G'TGCA_C
Cuts at: 5867 7023 5867
Size: 1156 6654
ApoI r'AATT_y
Cuts at: 1493 2756 4055 4862 5119 5345 5497 7606 1493
Size: 1263 1299 807 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1299 1263 807 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6364 160
Size: 872 5332 1606
Fragments arranged by size:
5332 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7810
AvaI C'yCGr_G
Cuts at: 1870 1943 2349 2451 3224 4377 4389 4997 5135
Size: 73 406 102 773 1153 12 608 138
Cuts at: 5135 1870
Size: 4545
Fragments arranged by size:
4545 1153 773 608 406 138 102 73
12
BamHI G'GATC_C
Cuts at: 3210 5140 5507 3210
Size: 1930 367 5513
Fragments arranged by size:
5513 1930 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2835 2886 2915 3919
Size: 325 217 627 555 493 51 29 1004
Cuts at: 3919 5131 6586 618
Size: 1212 1455 1842
Fragments arranged by size:
1842 1455 1212 1004 627 555 493 325
217 51 29
BanII G_rGCy'C
Cuts at: 1391 2050 2661 2877 2960 2970 3134 3183 3236
Size: 659 611 216 83 10 164 49 53
Cuts at: 3236 3615 4083 4396 4524 5129 1391
Size: 379 468 313 128 605 4072
Fragments arranged by size:
4072 659 611 605 468 379 313 216
164 128 83 53 49 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4325 928
Size: 971 2426 4413
Fragments arranged by size:
4413 2426 971
BbvCI CC'TCA_GC
Cuts at: 2303 3955 2303
Size: 1652 6158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4972 5006 6001 6035 1903
Size: 34 3035 34 995 34 3678
Fragments arranged by size:
3678 3035 995 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5702 6912 5702
Size: 1210 6600
BclI T'GATC_A
Cuts at: 1548 3576 1548
Size: 2028 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 2933 3683
Size: 107 122 71 631 605 366 895 750
Cuts at: 3683 4970 6421 136
Size: 1287 1451 1525
Fragments arranged by size:
1525 1451 1287 895 750 631 605 366
122 107 71
BglII A'GATC_T
Cuts at: 3256 7805 3256
Size: 4549 3261
BlpI GC'TnA_GC
Cuts at: 3410 4742 4940 5227 3410
Size: 1332 198 287 5993
Fragments arranged by size:
5993 1332 287 198
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2661 2918 2960 3134 3600 3615 4257
Size: 260 611 257 42 174 466 15 642
Cuts at: 4257 4396 4436 4770 5029 5871 7027 1790
Size: 139 40 334 259 842 1156 2573
Fragments arranged by size:
2573 1156 842 642 611 466 334 260
259 257 174 139 42 40 15
BmgBI CAC'GTC
Cuts at: 1318 4765 1318
Size: 3447 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4708 5541 6499
Size: 416 53 83 399 24 3291 833 958
Cuts at: 6499 442
Size: 1753
Fragments arranged by size:
3291 1753 958 833 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2662 3281 3704 3836 4025 4349 4361
Size: 1022 401 619 423 132 189 324 12
Cuts at: 4361 4901 6470 1239
Size: 540 1569 2579
Fragments arranged by size:
2579 1569 1022 619 540 423 401 324
189 132 12
Bpu10I CC'TnA_GC
Cuts at: 2042 2303 3955 4082 2042
Size: 261 1652 127 5770
Fragments arranged by size:
5770 1652 261 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2933 4772 5935 6800 7098 2933
Size: 1839 1163 865 298 3645
Fragments arranged by size:
3645 1839 1163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6473 882
Size: 617 4974 2219
Fragments arranged by size:
4974 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4198 5601 493
Size: 711 2994 1403 2702
Fragments arranged by size:
2994 2702 1403 711
BsaBI GATnn'nnATC
Cuts at: 7804 7804
Size: 7810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5617 5999 6634 275
Size: 53 83 186 936 4084 382 635 1451
Fragments arranged by size:
4084 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6243 6641 6915 7062 7473 6243
Size: 398 274 147 411 6580
Fragments arranged by size:
6580 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 3083 3113 3332
Size: 30 165 30 1182 30 468 30 219
Cuts at: 3332 3362 1178
Size: 30 5626
Fragments arranged by size:
5626 1182 468 219 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2558 2636 2639 2642 2645 2793 2810
Size: 1084 288 78 3 3 3 148 17
Cuts at: 2810 2873 2993 3023 3026 3029 3053 3075 3107
Size: 63 120 30 3 3 24 22 32
Cuts at: 3107 3226 3353 3356 4136 4163 4184 1186
Size: 119 127 3 780 27 21 4812
Fragments arranged by size:
4812 1084 780 288 148 127 120 119
78 63 32 30 27 24 22 21
17 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2475 3184 3565 3631 4265 4466 5048 7013 7509
Size: 709 381 66 634 201 582 1965 496
Cuts at: 7509 2475
Size: 2776
Fragments arranged by size:
2776 1965 709 634 582 496 381 201
66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4343 4436 4718 5021 5054 1163
Size: 573 2607 93 282 303 33 3919
Fragments arranged by size:
3919 2607 573 303 282 93 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2386 2677 3192 5260 6021 7049
Size: 275 186 385 291 515 2068 761 1028
Cuts at: 7049 1540
Size: 2301
Fragments arranged by size:
2301 2068 1028 761 515 385 291 275
186
BsiHKAI G_wGCw'C
Cuts at: 2530 2574 4083 5871 5956 7027 2530
Size: 44 1509 1788 85 1071 3313
Fragments arranged by size:
3313 1788 1509 1071 85 44
BsmI GAATG_Cn'
Cuts at: 3836 4686 5321 5414 3836
Size: 850 635 93 6232
Fragments arranged by size:
6232 850 635 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2661 2661
Size: 7810
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2530 2574 2661 2877 2918 2960
Size: 399 260 480 44 87 216 41 42
Cuts at: 2960 2970 3134 3183 3236 3600 3615 4083 4257
Size: 10 164 49 53 364 15 468 174
Cuts at: 4257 4396 4436 4524 4770 5029 5129 5871 5956
Size: 139 40 88 246 259 100 742 85
Cuts at: 5956 7027 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 480 468 399 364 260
259 246 216 174 164 139 100 88
87 85 53 49 44 42 41 40
15 10
BspEI T'CCGG_A
Cuts at: 7473 7473
Size: 7810
BspHI T'CATG_A
Cuts at: 5699 7429 5699
Size: 1730 6080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2835 3047 3294 3897 5165 844
Size: 1991 212 247 603 1268 3489
Fragments arranged by size:
3489 1991 1268 603 247 212
BsrBI CCG'CTC
Cuts at: 1394 1823 2580 2712 3409 3652 4351 4545 4885
Size: 429 757 132 697 243 699 194 340
Cuts at: 4885 5697 1394
Size: 812 3507
Fragments arranged by size:
3507 812 757 699 697 429 340 243
194 132
BsrDI GCAATG_nn'
Cuts at: 65 1248 6305 6479 65
Size: 1183 5057 174 1396
Fragments arranged by size:
5057 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3506 3906 6454 6641 1700
Size: 110 1696 400 2548 187 2869
Fragments arranged by size:
2869 2548 1696 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7810
BssHII G'CGCG_C
Cuts at: 1925 3753 1925
Size: 1828 5982
BssSI C'ACGA_G
Cuts at: 5870 6882 5870
Size: 1012 6798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4732 794
Size: 3938 3872
BstBI TT'CG_AA
Cuts at: 6614 6614
Size: 7810
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7810
BstXI CCAn_nnnn'nTGG
Cuts at: 5056 5056
Size: 7810
BstYI r'GATC_y
Cuts at: 1972 3210 3256 3459 5075 5140 5507 5892 5909
Size: 1238 46 203 1616 65 367 385 17
Cuts at: 5909 7361 7447 7459 7805 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1616 1452 1238 385 367 346 203
86 65 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 2976 3051 3087 2976
Size: 75 36 7699
Fragments arranged by size:
7699 75 36
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2380 2386 3415 3678 4561
Size: 552 939 198 178 6 1029 263 883
Cuts at: 4561 4749 4770 513
Size: 188 21 3553
Fragments arranged by size:
3553 1029 939 883 552 263 198 188
178 21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2442 3561 487
Size: 683 56 1216 1119 4736
Fragments arranged by size:
4736 1216 1119 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 3077 3116 3396 3524 3626 4434 5326 6120
Size: 855 39 280 128 102 808 892 794
Cuts at: 6120 6140 2222
Size: 20 3892
Fragments arranged by size:
3892 892 855 808 794 280 128 102
39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7810
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7775
DraI TTT'AAA
Cuts at: 5114 5114
Size: 7810
DraIII CAC_nnn'GTG
Cuts at: 2534 4414 4716 2534
Size: 1880 302 5628
Fragments arranged by size:
5628 1880 302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6817 809
Size: 507 5501 1802
Fragments arranged by size:
5501 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2383 3189 3675
Size: 54 1425 50 275 18 553 806 486
Cuts at: 3675 4296 5257 6146 7775 8
Size: 621 961 889 1629 43
Fragments arranged by size:
1629 1425 961 889 806 621 553 486
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2383 3189 5257 1537
Size: 275 571 806 2068 4090
Fragments arranged by size:
4090 2068 806 571 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2787 4015 4095 4170 4653 5740 1353
Size: 819 615 1228 80 75 483 1087 3423
Fragments arranged by size:
3423 1228 1087 819 615 483 80 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2677 3314 6397 6771 6917 387
Size: 1064 138 1088 637 3083 374 146 1280
Fragments arranged by size:
3083 1280 1088 1064 637 374 146 138
EcoICRI GAG'CTC
Cuts at: 4081 4081
Size: 7810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2662 2868 3281 3677 3704 3836
Size: 393 629 401 206 413 396 27 132
Cuts at: 3836 4025 4241 4349 4361 4595 4691 4901 5873
Size: 189 216 108 12 234 96 210 972
Cuts at: 5873 6470 7257 7525 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 972 787 629 597 413 401 396
393 268 234 216 210 206 189 132
108 96 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 3027 3054 3290 4790 2609
Size: 418 27 236 1500 5629
Fragments arranged by size:
5629 1500 418 236 27
EcoO109I rG'GnC_Cy
Cuts at: 2046 2047 2170 2433 2657 2658 2934 2956 2957
Size: 1 123 263 224 1 276 22 1
Cuts at: 2957 3047 3373 3445 3467 3611 3612 3781 4399
Size: 90 326 72 22 144 1 169 618
Cuts at: 4399 4426 4695 2046
Size: 27 269 5161
Fragments arranged by size:
5161 618 326 276 269 263 224 169
144 123 90 72 27 22 22 1
1 1 1
EcoRI G'AATT_C
Cuts at: 5119 5497 5119
Size: 378 7432
FspI TGC'GCA
Cuts at: 6316 6316
Size: 7810
HaeII r_GCGC'y
Cuts at: 1678 2602 2686 2846 3860 4132 4749 4950 6957
Size: 924 84 160 1014 272 617 201 2007
Cuts at: 6957 1678
Size: 2531
Fragments arranged by size:
2531 2007 1014 924 617 272 201 160
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3083 3115 4006 4038 4784
Size: 32 135 32 1511 32 891 32 746
Cuts at: 4784 4816 6451 6483 6525 6557 1373
Size: 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 1511 891 746 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5154 5400 669
Size: 988 281 3216 246 3079
Fragments arranged by size:
3216 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7810
HpaI GTT'AAC
Cuts at: 5400 5400
Size: 7810
KpnI G_GTAC'C
Cuts at: 5135 5135
Size: 7810
MfeI C'AATT_G
Cuts at: 5409 5409
Size: 7810
MluI A'CGCG_T
Cuts at: 7686 7686
Size: 7810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2985 6924 7108 1305
Size: 1680 3939 184 2007
Fragments arranged by size:
3939 2007 1680 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4298 10
Size: 54 1425 343 2466 3522
Fragments arranged by size:
3522 2466 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5768 6127 6286 518
Size: 648 4602 359 159 2042
Fragments arranged by size:
4602 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2248 2382 2397 2640 2679 3188 3217
Size: 598 44 134 15 243 39 509 29
Cuts at: 3217 3565 3680 3700 3724 4045 4339 4357 4512
Size: 348 115 20 24 321 294 18 155
Cuts at: 4512 4915 5226 5903 7051 7296 1606
Size: 403 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 598 509 403 348 321
311 294 245 243 155 134 115 44
39 29 24 20 18 15
NaeI GCC'GGC
Cuts at: 3508 3508
Size: 7810
NcoI C'CATG_G
Cuts at: 513 1065 2004 3415 4770 513
Size: 552 939 1411 1355 3553
Fragments arranged by size:
3553 1411 1355 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7810
NgoMIV G'CCGG_C
Cuts at: 3506 3506
Size: 7810
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7810
NotI GC'GGCC_GC
Cuts at: 5257 5257
Size: 7810
NspI r_CATG'y
Cuts at: 1132 1229 5168 6713 1132
Size: 97 3939 1545 2229
Fragments arranged by size:
3939 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3127 3370 3608 1348
Size: 1779 243 238 5550
Fragments arranged by size:
5550 1779 243 238
PciI A'CATG_T
Cuts at: 6709 6709
Size: 7810
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 3565 3990 1108
Size: 1896 561 425 4928
Fragments arranged by size:
4928 1896 561 425
PfoI T'CCnGG_A
Cuts at: 2172 4059 4326 4878 2172
Size: 1887 267 552 5104
Fragments arranged by size:
5104 1887 552 267
PmeI GTTT'AAAC
Cuts at: 5114 5114
Size: 7810
PmlI CAC'GTG
Cuts at: 4198 4198
Size: 7810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2617 3332 3364 5879 5911 7392 7424 2585
Size: 32 715 32 2515 32 1481 32 2971
Fragments arranged by size:
2971 2515 1481 715 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 3445 3781 2170
Size: 1275 336 6199
Fragments arranged by size:
6199 1275 336
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7810
PsiI TTA'TAA
Cuts at: 5380 5380
Size: 7810
PspOMI G'GGCC_C
Cuts at: 2046 2657 2956 3130 3611 4392 2046
Size: 611 299 174 481 781 5464
Fragments arranged by size:
5464 781 611 481 299 174
PstI C_TGCA'G
Cuts at: 830 2187 2214 2229 3172 3291 3324 3391 3705
Size: 1357 27 15 943 119 33 67 314
Cuts at: 3705 3846 4683 4959 5162 830
Size: 141 837 276 203 3478
Fragments arranged by size:
3478 1357 943 837 314 276 203 141
119 67 33 27 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7810
PvuII CAG'CTG
Cuts at: 2397 2640 3217 3700 3724 4045 4339 4357 2397
Size: 243 577 483 24 321 294 18 5850
Fragments arranged by size:
5850 577 483 321 294 243 24 18
SacI G_AGCT'C
Cuts at: 4083 4083
Size: 7810
SacII CC_GC'GG
Cuts at: 2205 2383 3681 2205
Size: 178 1298 6334
Fragments arranged by size:
6334 1298 178
SalI G'TCGA_C
Cuts at: 1936 5152 1936
Size: 3216 4594
SapI GCTCTTCn'nnn_
Cuts at: 2172 4170 2172
Size: 1998 5812
SbfI CC_TGCA'GG
Cuts at: 3324 5162 3324
Size: 1838 5972
ScaI AGT'ACT
Cuts at: 6058 7770 6058
Size: 1712 6098
SfcI C'TryA_G
Cuts at: 826 1045 2183 2210 2225 3168 3287 3320 3387
Size: 219 1138 27 15 943 119 33 67
Cuts at: 3387 3701 3842 4229 4679 4776 4955 5158 6293
Size: 314 141 387 450 97 179 203 1135
Cuts at: 6293 6974 7165 826
Size: 681 191 1471
Fragments arranged by size:
1471 1138 1135 943 681 450 387 314
219 203 191 179 141 119 97 67
33 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 3683 4970 3683
Size: 1287 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7810
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7810
SmaI CCC'GGG
Cuts at: 1872 2453 3226 4999 5137 1872
Size: 581 773 1773 138 4545
Fragments arranged by size:
4545 1773 773 581 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2948 4787 5914 6815 7077 820
Size: 197 926 1005 1839 1127 901 262 1553
Fragments arranged by size:
1839 1553 1127 1005 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5601 493
Size: 5108 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7810
SphI G_CATG'C
Cuts at: 1132 5168 1132
Size: 4036 3774
SspI AAT'ATT
Cuts at: 5 52 5734 5
Size: 47 5682 2081
Fragments arranged by size:
5682 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7810
StyI C'CwwG_G
Cuts at: 513 1065 2004 2561 2961 2998 3333 3415 3463
Size: 552 939 557 400 37 335 82 48
Cuts at: 3463 4770 5249 513
Size: 1307 479 3074
Fragments arranged by size:
3074 1307 939 557 552 479 400 335
82 48 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4279 4444 4727 5837 5854 6007 6192
Size: 1255 2699 165 283 1110 17 153 185
Cuts at: 6192 6637 325
Size: 445 1498
Fragments arranged by size:
2699 1498 1255 1110 445 283 185 165
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6056 7768 96
Size: 274 80 33 51 1443 4079 1712 138
Fragments arranged by size:
4079 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7770 7802 7770
Size: 32 7778
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7810
XbaI T'CTAG_A
Cuts at: 4030 5146 4030
Size: 1116 6694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 3526 4756 4876 2107
Size: 1419 1230 120 5041
Fragments arranged by size:
5041 1419 1230 120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7810
XmaI C'CCGG_G
Cuts at: 1870 2451 3224 4997 5135 1870
Size: 581 773 1773 138 4545
Fragments arranged by size:
4545 1773 773 581 138
XmnI GAAnn'nnTTC
Cuts at: 3434 5939 3434
Size: 2505 5305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5618 276
Size: 53 83 186 936 4084 2468
Fragments arranged by size:
4084 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SanDI SexAI SfoI
SrfI SwaI