Restriction map for
pFN21AE1023
(Circular) (Six-base) MAPSORT of: pFN21AE1023.seq Check: 4455 from: 1 to: 7027
pFN21AE1023 7027 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4240 4240
Size: 7027
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3182 4837 278
Size: 53 83 186 936 1646 1655 2468
Fragments arranged by size:
2468 1655 1646 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2520 4053 4370 1937
Size: 583 1533 317 4594
Fragments arranged by size:
4594 1533 583 317
Acc65I G'GTAC_C
Cuts at: 4348 4348
Size: 7027
AclI AA'CG_TT
Cuts at: 1563 5154 5527 1563
Size: 3591 373 3063
Fragments arranged by size:
3591 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2741 2945 5090 6474 6742 1632
Size: 1109 204 2145 1384 268 1917
Fragments arranged by size:
2145 1917 1384 1109 268 204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6830
AflIII A'CryG_T
Cuts at: 1203 4026 5926 6903 1203
Size: 2823 1900 977 1327
Fragments arranged by size:
2823 1900 1327 977
AgeI A'CCGG_T
Cuts at: 5858 5858
Size: 7027
AhdI GACnn_n'nnGTC
Cuts at: 5756 5756
Size: 7027
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2970 3584 1184
Size: 1786 614 4627
Fragments arranged by size:
4627 1786 614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 2316
Size: 32 6995
AlwNI CAG_nnn'CTG
Cuts at: 3758 4129 4770 6342 3758
Size: 371 641 1572 4443
Fragments arranged by size:
4443 1572 641 371
ApaI G_GGCC'C
Cuts at: 2294 2478 2976 3207 2294
Size: 184 498 231 6114
Fragments arranged by size:
6114 498 231 184
ApaLI G'TGCA_C
Cuts at: 5084 6240 5084
Size: 1156 5871
ApoI r'AATT_y
Cuts at: 1493 2559 4336 4562 4714 6823 1493
Size: 1066 1777 226 152 2109 1697
Fragments arranged by size:
2109 1777 1697 1066 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 4100 4179 4352 1870
Size: 73 2157 79 173 4545
Fragments arranged by size:
4545 2157 173 79 73
BamHI G'GATC_C
Cuts at: 4231 4357 4724 4231
Size: 126 367 6534
Fragments arranged by size:
6534 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4083 4252 4348 5803 618
Size: 325 217 627 2296 169 96 1455 1842
Fragments arranged by size:
2296 1842 1455 627 325 217 169 96
BanII G_rGCy'C
Cuts at: 1391 2082 2294 2432 2478 2553 2976 3207 3281
Size: 691 212 138 46 75 423 231 74
Cuts at: 3281 3992 4346 1391
Size: 711 354 4072
Fragments arranged by size:
4072 711 691 423 354 231 212 138
75 74 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 928
Size: 971 309 5747
Fragments arranged by size:
5747 971 309
BbvCI CC'TCA_GC
Cuts at: 3751 4014 3751
Size: 263 6764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3942 3976 5218 5252 1903
Size: 34 2005 34 1242 34 3678
Fragments arranged by size:
3678 2005 1242 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4919 6129 4919
Size: 1210 5817
BclI T'GATC_A
Cuts at: 1548 3564 1548
Size: 2016 5011
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2905 5638 136
Size: 107 122 71 631 605 1233 2733 1525
Fragments arranged by size:
2733 1525 1233 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2587 7022 2587
Size: 4435 2592
BlpI GC'TnA_GC
Cuts at: 4444 4444
Size: 7027
Bme1580I G_kGCm'C
Cuts at: 1790 2294 2478 2976 3207 4257 5088 6244 1790
Size: 504 184 498 231 1050 831 1156 2573
Fragments arranged by size:
2573 1156 1050 831 504 498 231 184
BmgBI CAC'GTC
Cuts at: 1318 4027 1318
Size: 2709 4318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2612 2976 3023
Size: 416 53 83 399 24 1195 364 47
Cuts at: 3023 4758 5716 442
Size: 1735 958 1753
Fragments arranged by size:
1753 1735 1195 958 416 399 364 83
53 47 24
BmtI G_CTAG'C
Cuts at: 1056 3960 1056
Size: 2904 4123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3409 3409
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2417 2988 3574 4041 4264 5687 1239
Size: 836 342 571 586 467 223 1423 2579
Fragments arranged by size:
2579 1423 836 586 571 467 342 223
Bpu10I CC'TnA_GC
Cuts at: 2654 3557 3751 4014 2654
Size: 903 194 263 5667
Fragments arranged by size:
5667 903 263 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 3306 5152 6017 6315 2612
Size: 694 1846 865 298 3324
Fragments arranged by size:
3324 1846 865 694 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5690 882
Size: 617 4191 2219
Fragments arranged by size:
4191 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4818 493
Size: 711 3614 2702
Fragments arranged by size:
3614 2702 711
BsaBI GATnn'nnATC
Cuts at: 7021 7021
Size: 7027
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3179 4281 4834 5216
Size: 53 83 186 936 1646 1102 553 382
Cuts at: 5216 5851 275
Size: 635 1451
Fragments arranged by size:
1646 1451 1102 936 635 553 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5460 5858 6132 6279 6690 5460
Size: 398 274 147 411 5797
Fragments arranged by size:
5797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6802
Fragments arranged by size:
6802 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2203 2561 2761 2991 3739 3754 4180 1186
Size: 1017 358 200 230 748 15 426 4033
Fragments arranged by size:
4033 1017 748 426 358 230 200 15
BseYI C'CCAG_C
Cuts at: 3236 3867 3972 4227 6230 6726 3236
Size: 631 105 255 2003 496 3537
Fragments arranged by size:
3537 2003 631 496 255 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2340 1163
Size: 573 604 5850
Fragments arranged by size:
5850 604 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4477 5238 6266 1540
Size: 275 186 2476 761 1028 2301
Fragments arranged by size:
2476 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2302 2553 3281 3992 5088 5173 6244 2302
Size: 251 728 711 1096 85 1071 3085
Fragments arranged by size:
3085 1096 1071 728 711 251 85
BsmI GAATG_Cn'
Cuts at: 4538 4631 4538
Size: 93 6934
BsmBI CGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 7027
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2294 2302 2432 2478 2553 2976
Size: 399 292 212 8 130 46 75 423
Cuts at: 2976 3207 3281 3992 4257 4346 5088 5173 6244
Size: 231 74 711 265 89 742 85 1071
Cuts at: 6244 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 711 423 399 292 265
231 212 130 89 85 75 74 46
8
BspEI T'CCGG_A
Cuts at: 6690 6690
Size: 7027
BspHI T'CATG_A
Cuts at: 4168 4916 6646 4168
Size: 748 1730 4549
Fragments arranged by size:
4549 1730 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 3729 4240 4382 844
Size: 1476 1409 511 142 3489
Fragments arranged by size:
3489 1476 1409 511 142
BsrBI CCG'CTC
Cuts at: 1394 1823 4259 4914 1394
Size: 429 2436 655 3507
Fragments arranged by size:
3507 2436 655 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 3196 4324 5522 5696 65
Size: 1183 1044 904 1128 1198 174 1396
Fragments arranged by size:
1396 1198 1183 1128 1044 904 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2512 5671 5858 1700
Size: 110 702 3159 187 2869
Fragments arranged by size:
3159 2869 702 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7027
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 2113 5087 6099 2113
Size: 2974 1012 3041
Fragments arranged by size:
3041 2974 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3856 794
Size: 3062 3965
BstBI TT'CG_AA
Cuts at: 5831 5831
Size: 7027
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 2744
Size: 7027
BstYI r'GATC_y
Cuts at: 1972 2587 2760 4009 4231 4357 4724 5109 5126
Size: 615 173 1249 222 126 367 385 17
Cuts at: 5126 6578 6664 6676 7022 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1249 615 385 367 346 222
173 126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 3603 3768 513
Size: 552 939 54 1545 165 3772
Fragments arranged by size:
3772 1545 939 552 165 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6288
Fragments arranged by size:
6288 683 56
BtsI GCAGTG_nn'
Cuts at: 3059 3756 3774 4543 5337 5357 3059
Size: 697 18 769 794 20 4729
Fragments arranged by size:
4729 794 769 697 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6992
DraI TTT'AAA
Cuts at: 4331 4331
Size: 7027
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6034 809
Size: 507 4718 1802
Fragments arranged by size:
4718 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 3771 4474
Size: 54 1425 50 275 18 550 1391 703
Cuts at: 4474 5363 6992 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1391 889 703 550 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4474 1537
Size: 275 2662 4090
Fragments arranged by size:
4090 2662 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2613 4957 1353
Size: 1260 2344 3423
Fragments arranged by size:
3423 2344 1260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5614 5988 6134 387
Size: 1064 138 4025 374 146 1280
Fragments arranged by size:
4025 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 3279 3990 2551
Size: 728 711 5588
Fragments arranged by size:
5588 728 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2417 2741 2945 2988 3574 4041
Size: 393 443 342 324 204 43 586 467
Cuts at: 4041 4264 5090 5687 6474 6742 1239
Size: 223 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 586 467 443 393
342 324 268 223 204 43
EcoNI CCTnn'n_nnAGG
Cuts at: 3003 3003
Size: 7027
EcoO109I rG'GnC_Cy
Cuts at: 2332 2474 2972 3203 3662 4126 2332
Size: 142 498 231 459 464 5233
Fragments arranged by size:
5233 498 464 459 231 142
EcoRI G'AATT_C
Cuts at: 4336 4714 4336
Size: 378 6649
FspI TGC'GCA
Cuts at: 5533 5533
Size: 7027
HaeII r_GCGC'y
Cuts at: 1678 4217 6174 1678
Size: 2539 1957 2531
Fragments arranged by size:
2539 2531 1957
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2335 2367 3325 3357 4292
Size: 32 135 32 763 32 958 32 935
Cuts at: 4292 4324 5668 5700 5742 5774 1373
Size: 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 958 935 763 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4371 4617 669
Size: 988 281 2433 246 3079
Fragments arranged by size:
3079 2433 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4067 748
Size: 3319 3708
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KpnI G_GTAC'C
Cuts at: 4352 4352
Size: 7027
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 6903 6903
Size: 7027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6141 6325 1305
Size: 4836 184 2007
Fragments arranged by size:
4836 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 10
Size: 54 1425 343 550 4655
Fragments arranged by size:
4655 1425 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 4985 5344 5503 518
Size: 648 2400 1419 359 159 2042
Fragments arranged by size:
2400 2042 1419 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2923 3055 3790 4227 4443 5120 6268 6513
Size: 1317 132 735 437 216 677 1148 245
Cuts at: 6513 1606
Size: 2120
Fragments arranged by size:
2120 1317 1148 735 677 437 245 216
132
NaeI GCC'GGC
Cuts at: 2514 2514
Size: 7027
NcoI C'CATG_G
Cuts at: 513 1065 2004 2058 513
Size: 552 939 54 5482
Fragments arranged by size:
5482 939 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7027
NgoMIV G'CCGG_C
Cuts at: 2512 2512
Size: 7027
NheI G'CTAG_C
Cuts at: 1052 3956 1052
Size: 2904 4123
NotI GC'GGCC_GC
Cuts at: 4474 4474
Size: 7027
NspI r_CATG'y
Cuts at: 1132 1229 2044 2752 3115 4385 5930 1132
Size: 97 815 708 363 1270 1545 2229
Fragments arranged by size:
2229 1545 1270 815 708 363 97
PasI CC'CwG_GG
Cuts at: 1348 3199 3359 4129 4218 1348
Size: 1851 160 770 89 4157
Fragments arranged by size:
4157 1851 770 160 89
PciI A'CATG_T
Cuts at: 5926 5926
Size: 7027
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4129 1108
Size: 3021 4006
PfoI T'CCnGG_A
Cuts at: 2091 3004 2091
Size: 913 6114
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 6609 6641 5096
Size: 32 1481 32 5482
Fragments arranged by size:
5482 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2332 3662 2332
Size: 1330 5697
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7027
PsiI TTA'TAA
Cuts at: 4597 4597
Size: 7027
PspOMI G'GGCC_C
Cuts at: 2290 2474 2972 3203 2290
Size: 184 498 231 6114
Fragments arranged by size:
6114 498 231 184
PstI C_TGCA'G
Cuts at: 830 2331 3726 3795 3849 3955 4379 830
Size: 1501 1395 69 54 106 424 3478
Fragments arranged by size:
3478 1501 1395 424 106 69 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 2923 3790 4227 2923
Size: 867 437 5723
Fragments arranged by size:
5723 867 437
SacI G_AGCT'C
Cuts at: 2553 3281 3992 2553
Size: 728 711 5588
Fragments arranged by size:
5588 728 711
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SanDI GG'GwC_CC
Cuts at: 3662 3662
Size: 7027
SapI GCTCTTCn'nnn_
Cuts at: 2613 2613
Size: 7027
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 2311 5275 6987 2311
Size: 2964 1712 2351
Fragments arranged by size:
2964 2351 1712
SfcI C'TryA_G
Cuts at: 826 1045 2327 2454 2517 3722 3791 3845 3951
Size: 219 1282 127 63 1205 69 54 106
Cuts at: 3951 4375 5510 6191 6382 826
Size: 424 1135 681 191 1471
Fragments arranged by size:
1471 1282 1205 1135 681 424 219 191
127 106 69 63 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 4354 1872
Size: 2482 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 3321 5131 6032 6294 820
Size: 197 926 648 730 1810 901 262 1553
Fragments arranged by size:
1810 1553 926 901 730 648 262 197
SnaBI TAC'GTA
Cuts at: 493 4818 493
Size: 4325 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7027
SphI G_CATG'C
Cuts at: 1132 2044 4385 1132
Size: 912 2341 3774
Fragments arranged by size:
3774 2341 912
SspI AAT'ATT
Cuts at: 5 52 4951 5
Size: 47 4899 2081
Fragments arranged by size:
4899 2081 47
StuI AGG'CCT
Cuts at: 1517 2260 3746 4241 1517
Size: 743 1486 495 4303
Fragments arranged by size:
4303 1486 743 495
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 4466 513
Size: 552 939 54 2408 3074
Fragments arranged by size:
3074 2408 939 552 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3060 5054 5071 5224 5409 5854 325
Size: 1255 1480 1994 17 153 185 445 1498
Fragments arranged by size:
1994 1498 1480 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2309 5273 6985
Size: 274 80 33 51 1443 332 2964 1712
Cuts at: 6985 96
Size: 138
Fragments arranged by size:
2964 1712 1443 332 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 6987 7019 2745
Size: 32 4210 32 2753
Fragments arranged by size:
4210 2753 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7027
XbaI T'CTAG_A
Cuts at: 4363 4363
Size: 7027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2901 2901
Size: 7027
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7027
XmaI C'CCGG_G
Cuts at: 1870 4352 1870
Size: 2482 4545
XmnI GAAnn'nnTTC
Cuts at: 5156 5156
Size: 7027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3180 4835 276
Size: 53 83 186 936 1646 1655 2468
Fragments arranged by size:
2468 1655 1646 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI