Restriction map for
pFN21AE1020
(Circular) (Six-base) MAPSORT of: pFN21AE1020.seq Check: 1121 from: 1 to: 6709
pFN21AE1020 6709 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3724 3826 3724
Size: 102 6607
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4519 278
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 2964 4052 1937
Size: 831 196 1088 4594
Fragments arranged by size:
4594 1088 831 196
Acc65I G'GTAC_C
Cuts at: 3956 4030 3956
Size: 74 6635
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2512 3246 3376 3505 3615 4772 6156
Size: 508 372 734 130 129 110 1157 1384
Cuts at: 6156 6424 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1157 734 508 372 268 130
129 110
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 2967 5608 6585 1203
Size: 1764 2641 977 1327
Fragments arranged by size:
2641 1764 1327 977
AgeI A'CCGG_T
Cuts at: 3273 5540 3273
Size: 2267 4442
AhdI GACnn_n'nnGTC
Cuts at: 2983 5438 2983
Size: 2455 4254
AleI CACnn'nnGTG
Cuts at: 1166 2282 1166
Size: 1116 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6709
AlwNI CAG_nnn'CTG
Cuts at: 2104 2467 2535 4452 6024 2104
Size: 363 68 1917 1572 2789
Fragments arranged by size:
2789 1917 1572 363 68
ApaI G_GGCC'C
Cuts at: 2512 3158 3710 2512
Size: 646 552 5511
Fragments arranged by size:
5511 646 552
ApaLI G'TGCA_C
Cuts at: 4766 5922 4766
Size: 1156 5553
ApoI r'AATT_y
Cuts at: 1493 2723 4018 4244 4396 6505 1493
Size: 1230 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 1230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2373 2502 3314 3336 3372 3703 4034
Size: 73 430 129 812 22 36 331 331
Cuts at: 4034 1870
Size: 4545
Fragments arranged by size:
4545 812 430 331 331 129 73 36
22
BamHI G'GATC_C
Cuts at: 4039 4406 4039
Size: 367 6342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3183 3245 3426 3666 3838
Size: 325 217 627 1396 62 181 240 172
Cuts at: 3838 3956 4030 5485 618
Size: 118 74 1455 1842
Fragments arranged by size:
1842 1455 1396 627 325 240 217 181
172 118 74 62
BanII G_rGCy'C
Cuts at: 1391 2512 2544 3026 3068 3158 3379 3588 3710
Size: 1121 32 482 42 90 221 209 122
Cuts at: 3710 3823 3908 4008 4028 1391
Size: 113 85 100 20 4072
Fragments arranged by size:
4072 1121 482 221 209 122 113 100
90 85 42 32 20
BbeI G_GCGC'C
Cuts at: 3187 3670 3187
Size: 483 6226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5738
BbvCI CC'TCA_GC
Cuts at: 2916 3131 3320 3974 2916
Size: 215 189 654 5651
Fragments arranged by size:
5651 654 215 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4900 4934 1903
Size: 34 2963 34 3678
Fragments arranged by size:
3678 2963 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4601 5811 4601
Size: 1210 5499
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2507 3120 3153
Size: 107 122 71 631 605 835 613 33
Cuts at: 3153 3855 3864 5320 136
Size: 702 9 1456 1525
Fragments arranged by size:
1525 1456 835 702 631 613 605 122
107 71 33 9
BglII A'GATC_T
Cuts at: 3898 6704 3898
Size: 2806 3903
BlpI GC'TnA_GC
Cuts at: 2327 4126 2327
Size: 1799 4910
Bme1580I G_kGCm'C
Cuts at: 1790 2512 2742 2835 3158 3250 3429 3649 3678
Size: 722 230 93 323 92 179 220 29
Cuts at: 3678 3710 3843 4770 5926 1790
Size: 32 133 927 1156 2573
Fragments arranged by size:
2573 1156 927 722 323 230 220 179
133 93 92 32 29
BmgBI CAC'GTC
Cuts at: 1318 2676 2968 1318
Size: 1358 292 5059
Fragments arranged by size:
5059 1358 292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2666 2822 3251
Size: 416 53 83 399 24 1249 156 429
Cuts at: 3251 3801 4011 4440 5398 442
Size: 550 210 429 958 1753
Fragments arranged by size:
1753 1249 958 550 429 429 416 399
210 156 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 3893 2528
Size: 1365 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2011 2738 3074 3487 3764 3837 3968 5369
Size: 772 727 336 413 277 73 131 1401
Cuts at: 5369 1239
Size: 2579
Fragments arranged by size:
2579 1401 772 727 413 336 277 131
73
Bpu10I CC'TnA_GC
Cuts at: 2634 2916 3131 3320 3403 3974 2634
Size: 282 215 189 83 571 5369
Fragments arranged by size:
5369 571 282 215 189 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3566 4834 5699 5997 2498
Size: 1068 1268 865 298 3210
Fragments arranged by size:
3210 1268 1068 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2991 5372 882
Size: 617 1492 2381 2219
Fragments arranged by size:
2381 2219 1492 617
BsaAI yAC'GTr
Cuts at: 493 1204 2805 3644 4500 493
Size: 711 1601 839 856 2702
Fragments arranged by size:
2702 1601 856 839 711
BsaBI GATnn'nnATC
Cuts at: 6703 6703
Size: 6709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3667 3925 4516
Size: 53 83 186 936 1651 483 258 591
Cuts at: 4516 4898 5533 275
Size: 382 635 1451
Fragments arranged by size:
1651 1451 936 635 591 483 382 258
186 83 53
BsaWI w'CCGG_w
Cuts at: 3273 5142 5540 5814 5961 6372 3273
Size: 1869 398 274 147 411 3610
Fragments arranged by size:
3610 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 3029 3059 1178
Size: 30 165 30 1395 30 201 30 4828
Fragments arranged by size:
4828 1395 201 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2901 3426 1186
Size: 1240 475 525 4469
Fragments arranged by size:
4469 1240 525 475
BseYI C'CCAG_C
Cuts at: 2051 2113 2219 2686 3352 5912 6408 2051
Size: 62 106 467 666 2560 496 2352
Fragments arranged by size:
2560 2352 666 496 467 106 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2656 3696 1163
Size: 573 920 1040 4176
Fragments arranged by size:
4176 1040 920 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4159 4920 5948 1540
Size: 275 186 2158 761 1028 2301
Fragments arranged by size:
2301 2158 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2316 2393 3823 3908 4770 4855 5926 2133
Size: 183 77 1430 85 862 85 1071 2916
Fragments arranged by size:
2916 1430 1071 862 183 85 85 77
BsmI GAATG_Cn'
Cuts at: 2365 4220 4313 2365
Size: 1855 93 4761
Fragments arranged by size:
4761 1855 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3932 3932
Size: 6709
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2316 2393 2512 2544 2742 2835
Size: 399 343 183 77 119 32 198 93
Cuts at: 2835 3026 3068 3158 3250 3379 3429 3588 3649
Size: 191 42 90 92 129 50 159 61
Cuts at: 3649 3678 3710 3823 3843 3908 4008 4028 4770
Size: 29 32 113 20 65 100 20 742
Cuts at: 4770 4855 5926 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 198 191 183
159 129 119 113 100 93 92 90
85 77 65 61 50 42 32 32
29 20 20
BspEI T'CCGG_A
Cuts at: 6372 6372
Size: 6709
BspHI T'CATG_A
Cuts at: 4598 6328 4598
Size: 1730 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 2780 3724 3826 4064 844
Size: 1685 251 944 102 238 3489
Fragments arranged by size:
3489 1685 944 251 238 102
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 2638 3204 4596 1394
Size: 429 211 604 566 1392 3507
Fragments arranged by size:
3507 1392 604 566 429 211
BsrDI GCAATG_nn'
Cuts at: 65 1248 5204 5378 65
Size: 1183 3956 174 1396
Fragments arranged by size:
3956 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 3119 3273 3429 3858 5353 5540
Size: 110 236 1073 154 156 429 1495 187
Cuts at: 5540 1700
Size: 2869
Fragments arranged by size:
2869 1495 1073 429 236 187 156 154
110
BsrGI T'GTAC_A
Cuts at: 96 2567 96
Size: 2471 4238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6709
BssSI C'ACGA_G
Cuts at: 2615 2932 3043 4769 5781 2615
Size: 317 111 1726 1012 3543
Fragments arranged by size:
3543 1726 1012 317 111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 794
Size: 1310 5399
BstBI TT'CG_AA
Cuts at: 5513 5513
Size: 6709
BstEII G'GTnAC_C
Cuts at: 1184 2149 3008 1184
Size: 965 859 4885
Fragments arranged by size:
4885 965 859
BstYI r'GATC_y
Cuts at: 1972 3898 4039 4406 4791 4808 6260 6346 6358
Size: 1926 141 367 385 17 1452 86 12
Cuts at: 6358 6704 1972
Size: 346 1977
Fragments arranged by size:
1977 1926 1452 385 367 346 141 86
17 12
BstZ17I GTA'TAC
Cuts at: 2769 2769
Size: 6709
BtgI C'CryG_G
Cuts at: 513 1065 2817 2973 3078 3179 3191 3470 513
Size: 552 1752 156 105 101 12 279 3752
Fragments arranged by size:
3752 1752 552 279 156 105 101 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5970
Fragments arranged by size:
5970 683 56
BtsI GCAGTG_nn'
Cuts at: 3510 4225 5019 5039 3510
Size: 715 794 20 5180
Fragments arranged by size:
5180 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2890 2925 529
Size: 35 2326 35 4313
Fragments arranged by size:
4313 2326 35 35
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DraIII CAC_nnn'GTG
Cuts at: 3245 3245
Size: 6709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5716 809
Size: 507 4400 1802
Fragments arranged by size:
4400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2270 3287 3749
Size: 54 1425 50 275 18 440 1017 462
Cuts at: 3749 3865 3937 4156 5045 6674 8
Size: 116 72 219 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 462 440 275 219
116 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4156 1537
Size: 275 2344 4090
Fragments arranged by size:
4090 2344 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 2531 3611 3919 4639 1353
Size: 823 355 1080 308 720 3423
Fragments arranged by size:
3423 1080 823 720 355 308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2066 3599 5296 5670 5816 387
Size: 1064 138 477 1533 1697 374 146 1280
Fragments arranged by size:
1697 1533 1280 1064 477 374 146 138
EcoICRI GAG'CTC
Cuts at: 3821 3906 3821
Size: 85 6624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2011 2140 2512 2738 3074 3246 3376
Size: 393 379 129 372 226 336 172 130
Cuts at: 3376 3487 3505 3615 3764 3837 3968 4772 5369
Size: 111 18 110 149 73 131 804 597
Cuts at: 5369 6156 6424 1239
Size: 787 268 1524
Fragments arranged by size:
1524 804 787 597 393 379 372 336
268 226 172 149 131 130 129 111
110 73 18
EcoO109I rG'GnC_Cy
Cuts at: 2062 2101 2202 2508 2509 2660 3154 3155 3302
Size: 39 101 306 1 151 494 1 147
Cuts at: 3302 3331 3526 3707 3733 3760 2062
Size: 29 195 181 26 27 5011
Fragments arranged by size:
5011 494 306 195 181 151 147 101
39 29 27 26 1 1
EcoRI G'AATT_C
Cuts at: 4018 4396 4018
Size: 378 6331
EcoRV GAT'ATC
Cuts at: 3786 3786
Size: 6709
FseI GG_CCGG'CC
Cuts at: 3862 3862
Size: 6709
FspI TGC'GCA
Cuts at: 5215 5215
Size: 6709
HaeII r_GCGC'y
Cuts at: 1678 3187 3670 5856 1678
Size: 1509 483 2186 2531
Fragments arranged by size:
2531 2186 1509 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2969 3001 3805 3837 3890
Size: 32 135 32 1397 32 804 32 53
Cuts at: 3890 3922 5350 5382 5424 5456 1373
Size: 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 1397 804 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4053 4299 669
Size: 988 281 2115 246 3079
Fragments arranged by size:
3079 2115 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6709
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KasI G'GCGC_C
Cuts at: 3183 3666 3183
Size: 483 6226
KpnI G_GTAC'C
Cuts at: 3960 4034 3960
Size: 74 6635
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 6585 6585
Size: 6709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2407 3015 3258 5823 6007 1305
Size: 1102 608 243 2565 184 2007
Fragments arranged by size:
2565 2007 1102 608 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3751 10
Size: 54 1425 343 1919 2968
Fragments arranged by size:
2968 1919 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2282 4667 5026 5185 518
Size: 648 978 138 2385 359 159 2042
Fragments arranged by size:
2385 2042 978 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2982 3257 3267 3460 3621 3747 3891 4125
Size: 1376 275 10 193 161 126 144 234
Cuts at: 4125 4802 5950 6195 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1376 1148 677 275 245 234 193
161 144 126 10
NaeI GCC'GGC
Cuts at: 2048 3121 3860 2048
Size: 1073 739 4897
Fragments arranged by size:
4897 1073 739
NarI GG'CG_CC
Cuts at: 3184 3667 3184
Size: 483 6226
NcoI C'CATG_G
Cuts at: 513 1065 2817 2973 513
Size: 552 1752 156 4249
Fragments arranged by size:
4249 1752 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6709
NgoMIV G'CCGG_C
Cuts at: 2046 3119 3858 2046
Size: 1073 739 4897
Fragments arranged by size:
4897 1073 739
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6709
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 4067 5612 1132
Size: 97 2838 1545 2229
Fragments arranged by size:
2838 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3017 3294 3727 1348
Size: 1669 277 433 4330
Fragments arranged by size:
4330 1669 433 277
PciI A'CATG_T
Cuts at: 5608 5608
Size: 6709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3277 3727 1108
Size: 2169 450 4090
Fragments arranged by size:
4090 2169 450
PfoI T'CCnGG_A
Cuts at: 2261 3788 2261
Size: 1527 5182
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 6291 6323 4778
Size: 32 1481 32 5164
Fragments arranged by size:
5164 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2202 2660 3302 3331 3526 3733 2202
Size: 458 642 29 195 207 5178
Fragments arranged by size:
5178 642 458 207 195 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6709
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PspOMI G'GGCC_C
Cuts at: 2508 3154 3706 2508
Size: 646 552 5511
Fragments arranged by size:
5511 646 552
PstI C_TGCA'G
Cuts at: 830 2100 2777 3063 3577 4061 830
Size: 1270 677 286 514 484 3478
Fragments arranged by size:
3478 1270 677 514 484 286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 3257 3267 3747 3891 3257
Size: 10 480 144 6075
Fragments arranged by size:
6075 480 144 10
SacI G_AGCT'C
Cuts at: 3823 3908 3823
Size: 85 6624
SalI G'TCGA_C
Cuts at: 1936 4051 1936
Size: 2115 4594
SanDI GG'GwC_CC
Cuts at: 3302 3331 3302
Size: 29 6680
SapI GCTCTTCn'nnn_
Cuts at: 2531 3919 2531
Size: 1388 5321
SbfI CC_TGCA'GG
Cuts at: 4061 4061
Size: 6709
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SfcI C'TryA_G
Cuts at: 826 1045 2096 2773 3059 3573 4057 5192 5873
Size: 219 1051 677 286 514 484 1135 681
Cuts at: 5873 6064 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1051 681 677 514 484 286
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3153 3864 3153
Size: 711 5998
SfoI GGC'GCC
Cuts at: 3185 3668 3185
Size: 483 6226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 3338 4036 1872
Size: 1466 698 4545
Fragments arranged by size:
4545 1466 698
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3545 4813 5714 5976 820
Size: 197 926 570 1032 1268 901 262 1553
Fragments arranged by size:
1553 1268 1032 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 4067 1132
Size: 2935 3774
SspI AAT'ATT
Cuts at: 5 52 4633 5
Size: 47 4581 2081
Fragments arranged by size:
4581 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6709
StyI C'CwwG_G
Cuts at: 513 1065 2817 2973 4148 513
Size: 552 1752 156 1175 3074
Fragments arranged by size:
3074 1752 1175 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3233 3286 4736 4753 4906 5091 5536
Size: 1255 1653 53 1450 17 153 185 445
Cuts at: 5536 325
Size: 1498
Fragments arranged by size:
1653 1498 1450 1255 445 185 153 53
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2567 4955 6667
Size: 274 80 33 51 1443 590 2388 1712
Cuts at: 6667 96
Size: 138
Fragments arranged by size:
2388 1712 1443 590 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 6669 6701 3029
Size: 32 3608 32 3037
Fragments arranged by size:
3608 3037 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6709
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 2713
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6709
XmaI C'CCGG_G
Cuts at: 1870 3336 4034 1870
Size: 1466 698 4545
Fragments arranged by size:
4545 1466 698
XmnI GAAnn'nnTTC
Cuts at: 4838 4838
Size: 6709
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4517 276
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
Bsu36I EcoNI FalI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SrfI SwaI