Restriction map for
pFN21AE1017
(Circular) (Six-base) MAPSORT of: pFN21AE1017.seq Check: 3160 from: 1 to: 6124
pFN21AE1017 6124 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2976 2979 3205 3290 4187 5571 5839
Size: 786 558 3 226 85 897 1384 268
Cuts at: 5839 1632
Size: 1917
Fragments arranged by size:
1917 1384 897 786 558 268 226 85
3
AfeI AGC'GCT
Cuts at: 3260 3260
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 5023 6000 1203
Size: 3820 977 1327
Fragments arranged by size:
3820 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 2683 4853 2683
Size: 2170 3954
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 6092
AlwNI CAG_nnn'CTG
Cuts at: 3867 5439 3867
Size: 1572 4552
ApaLI G'TGCA_C
Cuts at: 2805 4181 5337 2805
Size: 1376 1156 3592
Fragments arranged by size:
3592 1376 1156
ApoI r'AATT_y
Cuts at: 1493 2412 2445 2628 3433 3659 3811 5920 1493
Size: 919 33 183 805 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 805 226 183 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3445 4900 618
Size: 325 217 627 1658 1455 1842
Fragments arranged by size:
1842 1658 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2588 3313 3443 1391
Size: 797 400 725 130 4072
Fragments arranged by size:
4072 797 725 400 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3302 928
Size: 971 1403 3750
Fragments arranged by size:
3750 1403 971
BbvCI CC'TCA_GC
Cuts at: 2709 2709
Size: 6124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2995 4735 136
Size: 107 122 71 631 605 1323 1740 1525
Fragments arranged by size:
1740 1525 1323 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 2809 4185 5341 1790
Size: 1019 1376 1156 2573
Fragments arranged by size:
2573 1376 1156 1019
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 2621 3855
Size: 416 53 83 399 24 1117 87 1234
Cuts at: 3855 4813 442
Size: 958 1753
Fragments arranged by size:
1753 1234 1117 958 416 399 87 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2934 2967 3327 4784 1239
Size: 1049 646 33 360 1457 2579
Fragments arranged by size:
2579 1457 1049 646 360 33
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 6124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 4249 5114 5412 2834
Size: 1415 865 298 3546
Fragments arranged by size:
3546 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4787 882
Size: 617 3288 2219
Fragments arranged by size:
3288 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2323 3931 4313 4948
Size: 53 83 186 936 790 1608 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1608 1451 936 790 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4557 4955 5229 5376 5787 4557
Size: 398 274 147 411 4894
Fragments arranged by size:
4894 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2761 2791 2963 2993 1178
Size: 30 165 30 1358 30 172 30 4309
Fragments arranged by size:
4309 1358 172 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2663 2666 2669 2697 2900 2931 1186
Size: 1477 3 3 28 203 31 4379
Fragments arranged by size:
4379 1477 203 31 28 3 3
BseYI C'CCAG_C
Cuts at: 2056 3396 5327 5823 2056
Size: 1340 1931 496 2357
Fragments arranged by size:
2357 1931 1340 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2817 1163
Size: 573 450 631 4470
Fragments arranged by size:
4470 631 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2408 2809 3291 4185 4270 5341 2408
Size: 401 482 894 85 1071 3191
Fragments arranged by size:
3191 1071 894 482 401 85
BsmI GAATG_Cn'
Cuts at: 2514 3635 3728 2514
Size: 1121 93 4910
Fragments arranged by size:
4910 1121 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2261 2330 2261
Size: 69 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2408 2588 2809 3291 3313 3443
Size: 399 398 220 180 221 482 22 130
Cuts at: 3443 4185 4270 5341 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 398 221 220
180 130 85 22
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2380 3479 844
Size: 1213 323 1099 3489
Fragments arranged by size:
3489 1213 1099 323
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3201 4768 4955 1700
Size: 110 1391 1567 187 2869
Fragments arranged by size:
2869 1567 1391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3211 794
Size: 2417 3707
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 3043 3454 3821 4206 4223 5675 5761 5773
Size: 1071 411 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1071 411 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2880 2880
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2004 2063 2455 513
Size: 552 939 59 392 4182
Fragments arranged by size:
4182 939 552 392 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 2759 2834 3189 3402 3640 4434 4454 2202
Size: 557 75 355 213 238 794 20 3872
Fragments arranged by size:
3872 794 557 355 238 213 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 3334 3571
Size: 54 1425 50 275 18 537 967 237
Cuts at: 3571 4460 6089 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 967 889 537 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 2527 2640 4054 1353
Size: 999 175 113 1414 3423
Fragments arranged by size:
3423 1414 999 175 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2318 3356 4711 5085 5231 387
Size: 1064 138 729 1038 1355 374 146 1280
Fragments arranged by size:
1355 1280 1064 1038 729 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2418 2934 2967 2976 2979 3205
Size: 393 656 130 516 33 9 3 226
Cuts at: 3205 3290 3327 4187 4784 5571 5839 1239
Size: 85 37 860 597 787 268 1524
Fragments arranged by size:
1524 860 787 656 597 516 393 268
226 130 85 37 33 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 2592 3383 2165
Size: 427 791 4906
Fragments arranged by size:
4906 791 427
EcoO109I rG'GnC_Cy
Cuts at: 3080 3376 3080
Size: 296 5828
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
EcoRV GAT'ATC
Cuts at: 2892 2892
Size: 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2589 2589
Size: 6124
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 3262 5271 1678
Size: 1584 2009 2531
Fragments arranged by size:
2531 2009 1584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 2669 2701 2761
Size: 32 135 32 818 32 247 32 60
Cuts at: 2761 2793 2876 2908 4765 4797 4839 4871 1373
Size: 32 83 32 1857 32 42 32 2626
Fragments arranged by size:
2626 1857 818 247 135 83 60 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2786 748
Size: 2038 4086
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 10
Size: 54 1425 343 537 3765
Fragments arranged by size:
3765 1425 537 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3054 4082 4441 4600 518
Size: 648 1888 1028 359 159 2042
Fragments arranged by size:
2042 1888 1028 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3540 4217 5365 5610 1606
Size: 1934 677 1148 245 2120
Fragments arranged by size:
2120 1934 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 3320 3482 5027 1132
Size: 97 2091 162 1545 2229
Fragments arranged by size:
2229 2091 1545 162 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3011 1108
Size: 1903 4221
PfoI T'CCnGG_A
Cuts at: 3029 3029
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2126 2158 2963 2995 4193 4225 5706 5738 2126
Size: 32 805 32 1198 32 1481 32 2512
Fragments arranged by size:
2512 1481 1198 805 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3080 3376 3080
Size: 296 5828
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 3142 3476 830
Size: 2312 334 3478
Fragments arranged by size:
3478 2312 334
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SapI GCTCTTCn'nnn_
Cuts at: 2352 2527 2352
Size: 175 5949
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 2499 3138 3472 4607 5288 5479 826
Size: 219 1454 639 334 1135 681 191 1471
Fragments arranged by size:
1471 1454 1135 681 639 334 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2813 4228 5129 5391 820
Size: 197 926 870 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 870 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3320 3482 1132
Size: 2188 162 3774
Fragments arranged by size:
3774 2188 162
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 2004 3563 513
Size: 552 939 1559 3074
Fragments arranged by size:
3074 1559 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2772 4151 4168 4321 4506 4951 325
Size: 1255 1192 1379 17 153 185 445 1498
Fragments arranged by size:
1498 1379 1255 1192 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2542 4370 6082
Size: 274 80 33 51 1443 565 1828 1712
Cuts at: 6082 96
Size: 138
Fragments arranged by size:
1828 1712 1443 565 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 6084 6116 2055
Size: 32 3997 32 2063
Fragments arranged by size:
3997 2063 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3104 3104
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI