Restriction map for
pFN21AE1016
(Circular) (Six-base) MAPSORT of: pFN21AE1016.seq Check: 4597 from: 1 to: 6025
pFN21AE1016 6025 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 3368 1937
Size: 1113 318 4594
Fragments arranged by size:
4594 1113 318
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 2721 4152 4525 1563
Size: 1158 1431 373 3063
Fragments arranged by size:
3063 1431 1158 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 2735 3155 4088 5472 5740 1632
Size: 424 679 420 933 1384 268 1917
Fragments arranged by size:
1917 1384 933 679 424 420 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2384 1184
Size: 1200 4825
AlwNI CAG_nnn'CTG
Cuts at: 2079 2436 2553 2681 2871 2923 3173 3208 3768
Size: 357 117 128 190 52 250 35 560
Cuts at: 3768 5340 2079
Size: 1572 2764
Fragments arranged by size:
2764 1572 560 357 250 190 128 117
52 35
ApaLI G'TGCA_C
Cuts at: 2369 4082 5238 2369
Size: 1713 1156 3156
Fragments arranged by size:
3156 1713 1156
ApoI r'AATT_y
Cuts at: 1493 2707 3334 3560 3712 5821 1493
Size: 1214 627 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 627 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3350 1870
Size: 73 147 1260 4545
Fragments arranged by size:
4545 1260 147 73
BamHI G'GATC_C
Cuts at: 2695 3355 3722 2695
Size: 660 367 4998
Fragments arranged by size:
4998 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2353 2523 2909 2964 3346
Size: 325 217 627 566 170 386 55 382
Cuts at: 3346 4801 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 566 386 382 325 217
170 55
BanII G_rGCy'C
Cuts at: 1391 2627 3344 1391
Size: 1236 717 4072
Fragments arranged by size:
4072 1236 717
BbeI G_GCGC'C
Cuts at: 2357 2913 2968 2357
Size: 556 55 5414
Fragments arranged by size:
5414 556 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 928
Size: 971 1122 3932
Fragments arranged by size:
3932 1122 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2757 4636 136
Size: 107 122 71 631 605 1085 1879 1525
Fragments arranged by size:
1879 1525 1085 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 2147 3442 2147
Size: 1295 4730
Bme1580I G_kGCm'C
Cuts at: 1790 2373 2493 2526 4086 5242 1790
Size: 583 120 33 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 583 120 33
BmgBI CAC'GTC
Cuts at: 1318 2630 1318
Size: 1312 4713
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 3214 2557
Size: 657 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 2805 3047 4685 1239
Size: 1402 164 242 1638 2579
Fragments arranged by size:
2579 1638 1402 242 164
Bpu10I CC'TnA_GC
Cuts at: 2346 2747 2346
Size: 401 5624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4688 882
Size: 617 3189 2219
Fragments arranged by size:
3189 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 3816 493
Size: 711 1068 1544 2702
Fragments arranged by size:
2702 1544 1068 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2354 2910 2965
Size: 53 83 186 936 476 345 556 55
Cuts at: 2965 3832 4214 4849 275
Size: 867 382 635 1451
Fragments arranged by size:
1451 936 867 635 556 476 382 345
186 83 55 53
BsaWI w'CCGG_w
Cuts at: 2559 4458 4856 5130 5277 5688 2559
Size: 1899 398 274 147 411 2896
Fragments arranged by size:
2896 1899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2614 2644 1178
Size: 30 165 30 1211 30 4559
Fragments arranged by size:
4559 1211 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2625 2855 3018 1186
Size: 1439 230 163 4193
Fragments arranged by size:
4193 1439 230 163
BseYI C'CCAG_C
Cuts at: 2510 2882 5228 5724 2510
Size: 372 2346 496 2811
Fragments arranged by size:
2811 2346 496 372
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2449 2661 1163
Size: 573 713 212 4527
Fragments arranged by size:
4527 713 573 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2347 2373 2787 4086 4171 5242 2347
Size: 26 414 1299 85 1071 3130
Fragments arranged by size:
3130 1299 1071 414 85 26
BsmI GAATG_Cn'
Cuts at: 2504 3536 3629 2504
Size: 1032 93 4900
Fragments arranged by size:
4900 1032 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 2821
Size: 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 2373 2493 2526 2627 2787 3344
Size: 399 557 26 120 33 101 160 557
Cuts at: 3344 4086 4171 5242 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 557 399 160 120
101 85 33 26
BspEI T'CCGG_A
Cuts at: 2559 5688 2559
Size: 3129 2896
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 3281 3912 1394
Size: 429 317 1141 631 3507
Fragments arranged by size:
3507 1141 631 429 317
BsrDI GCAATG_nn'
Cuts at: 65 1248 2404 4520 4694 65
Size: 1183 1156 2116 174 1396
Fragments arranged by size:
2116 1396 1183 1156 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 4669 4856 1700
Size: 110 509 2350 187 2869
Fragments arranged by size:
2869 2350 509 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 2054 1925
Size: 129 5896
BssSI C'ACGA_G
Cuts at: 2637 4085 5097 2637
Size: 1448 1012 3565
Fragments arranged by size:
3565 1448 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2436 2871 794
Size: 1642 435 3948
Fragments arranged by size:
3948 1642 435
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2619 2619
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2695 3355 3722 4107 4124 5576 5662 5674
Size: 723 660 367 385 17 1452 86 12
Cuts at: 5674 6020 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 723 660 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2097 2970 2097
Size: 873 5152
BtgI C'CryG_G
Cuts at: 513 1065 2004 2664 3179 3213 513
Size: 552 939 660 515 34 3325
Fragments arranged by size:
3325 939 660 552 515 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2223 2403 487
Size: 683 56 997 180 4109
Fragments arranged by size:
4109 997 683 180 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 2775 1554
Size: 1221 4804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2875 2910 3021 3056 529
Size: 35 2311 35 111 35 3498
Fragments arranged by size:
3498 2311 111 35 35 35
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 2342 2342
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3025 5032 809
Size: 507 1709 2007 1802
Fragments arranged by size:
2007 1802 1709 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2259 2588
Size: 54 1425 50 275 18 327 102 329
Cuts at: 2588 3472 4361 5990 8
Size: 884 889 1629 43
Fragments arranged by size:
1629 1425 889 884 329 327 275 102
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 3955 1353
Size: 1487 1115 3423
Fragments arranged by size:
3423 1487 1115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 2942 4612 4986 5132 387
Size: 1064 138 528 825 1670 374 146 1280
Fragments arranged by size:
1670 1280 1064 825 528 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2641 2735 2805 3047 3155 4088
Size: 393 424 585 94 70 242 108 933
Cuts at: 4088 4685 5472 5740 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 933 787 597 585 424 393 268
242 108 94 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2409 2531 2640 2409
Size: 122 109 5794
Fragments arranged by size:
5794 122 109
EcoO109I rG'GnC_Cy
Cuts at: 2129 3122 2129
Size: 993 5032
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
EcoRV GAT'ATC
Cuts at: 2773 2773
Size: 6025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 6025
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 2357 2913 2968 5172 1678
Size: 679 556 55 2204 2531
Fragments arranged by size:
2531 2204 679 556 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2614 2646 4666 4698 4740
Size: 32 135 32 1042 32 2020 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 2020 1042 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KasI G'GCGC_C
Cuts at: 2353 2909 2964 2353
Size: 556 55 5414
Fragments arranged by size:
5414 556 55
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 5139 5323 1305
Size: 1662 2172 184 2007
Fragments arranged by size:
2172 2007 1662 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2590 10
Size: 54 1425 343 429 329 3445
Fragments arranged by size:
3445 1425 429 343 329 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2376 3983 4342 4501 518
Size: 648 1210 1607 359 159 2042
Fragments arranged by size:
2042 1607 1210 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2553 2741 2868 3181 3441 4118 5266 5511
Size: 947 188 127 313 260 677 1148 245
Cuts at: 5511 1606
Size: 2120
Fragments arranged by size:
2120 1148 947 677 313 260 245 188
127
NaeI GCC'GGC
Cuts at: 2321 2321
Size: 6025
NarI GG'CG_CC
Cuts at: 2354 2910 2965 2354
Size: 556 55 5414
Fragments arranged by size:
5414 556 55
NcoI C'CATG_G
Cuts at: 513 1065 2004 3213 513
Size: 552 939 1209 3325
Fragments arranged by size:
3325 1209 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NgoMIV G'CCGG_C
Cuts at: 2319 2319
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 3383 4928 1132
Size: 97 2154 1545 2229
Fragments arranged by size:
2229 2154 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PfoI T'CCnGG_A
Cuts at: 2125 2125
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 3122 2129
Size: 993 5032
PshAI GACnn'nnGTC
Cuts at: 1613 2813 1613
Size: 1200 4825
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2087 3258 3377 830
Size: 1257 1171 119 3478
Fragments arranged by size:
3478 1257 1171 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2741 2868 2741
Size: 127 5898
SacII CC_GC'GG
Cuts at: 3182 3182
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SanDI GG'GwC_CC
Cuts at: 2129 3122 2129
Size: 993 5032
SapI GCTCTTCn'nnn_
Cuts at: 2840 2840
Size: 6025
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2083 3051 3071 3254 3373 4508 5189
Size: 219 1038 968 20 183 119 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1038 968 681 219 191 183
119 20
SfoI GGC'GCC
Cuts at: 2355 2911 2966 2355
Size: 556 55 5414
Fragments arranged by size:
5414 556 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2027 1517
Size: 510 5515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2949 3213 3464 513
Size: 552 939 945 264 251 3074
Fragments arranged by size:
3074 945 939 552 264 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4052 4069 4222 4407 4852 325
Size: 1255 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5985 6017 5985
Size: 32 5993
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2616 2920 2616
Size: 304 5721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstZ17I EcoICRI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SexAI
SfiI SrfI SwaI