Restriction map for
pFN21AE1007
(Circular) (Six-base) MAPSORT of: pFN21AE1007.seq Check: 4386 from: 1 to: 5293
pFN21AE1007 5293 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2347 2428 2347
Size: 81 5212
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2376 3103 278
Size: 53 83 186 936 840 727 2468
Fragments arranged by size:
2468 936 840 727 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2636 1937
Size: 274 425 4594
Fragments arranged by size:
4594 425 274
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 2592 3420 3793 1563
Size: 1029 828 373 3063
Fragments arranged by size:
3063 1029 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 2563 4022 2563
Size: 1459 3834
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 2368 3036 4608 2368
Size: 668 1572 3053
Fragments arranged by size:
3053 1572 668
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 2618 1870
Size: 73 567 108 4545
Fragments arranged by size:
4545 567 108 73
BamHI G'GATC_C
Cuts at: 2623 2990 2623
Size: 367 4926
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2516 2614 4069 618
Size: 325 217 627 729 98 1455 1842
Fragments arranged by size:
1842 1455 729 627 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2408 2540 2612 1391
Size: 1017 132 72 4072
Fragments arranged by size:
4072 1017 132 72
BbeI G_GCGC'C
Cuts at: 2520 2520
Size: 5293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 3904 136
Size: 107 122 71 631 605 394 1838 1525
Fragments arranged by size:
1838 1525 631 605 394 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2498 2710 2498
Size: 212 5081
Bme1580I G_kGCm'C
Cuts at: 1790 2140 3354 4510 1790
Size: 350 1214 1156 2573
Fragments arranged by size:
2573 1214 1156 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 3982 442
Size: 416 53 83 399 24 1607 958 1753
Fragments arranged by size:
1753 1607 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2536 2095
Size: 441 4852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2501 3953 1239
Size: 830 432 1452 2579
Fragments arranged by size:
2579 1452 830 432
Bpu10I CC'TnA_GC
Cuts at: 2303 2303
Size: 5293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2605 3418 4283 4581 2605
Size: 813 865 298 3317
Fragments arranged by size:
3317 865 813 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2332 3956 882
Size: 617 833 1624 2219
Fragments arranged by size:
2219 1624 833 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2201 2373 2517 3100
Size: 53 83 186 936 668 172 144 583
Cuts at: 3100 3482 4117 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 668 635 583 382 186 172
144 83 53
BsaWI w'CCGG_w
Cuts at: 3726 4124 4398 4545 4956 3726
Size: 398 274 147 411 4063
Fragments arranged by size:
4063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2320 2483 1186
Size: 1134 163 3996
Fragments arranged by size:
3996 1134 163
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 2381 1163
Size: 573 516 129 4075
Fragments arranged by size:
4075 573 516 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3504 4532 1540
Size: 275 186 742 761 1028 2301
Fragments arranged by size:
2301 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 2309 2408 2540 3354 3439 4510 2309
Size: 99 132 814 85 1071 3092
Fragments arranged by size:
3092 1071 814 132 99 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2309 2408 2540 2612 3354 3439
Size: 399 350 169 99 132 72 742 85
Cuts at: 3439 4510 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 169 132 99
85 72
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2347 2428 2648 844
Size: 1481 22 81 220 3489
Fragments arranged by size:
3489 1481 220 81 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 1394
Size: 429 1357 3507
Fragments arranged by size:
3507 1357 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3937 4124 1700
Size: 110 2127 187 2869
Fragments arranged by size:
2869 2127 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2359 794
Size: 1565 3728
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 2257 2278 1184
Size: 1073 21 4199
Fragments arranged by size:
4199 1073 21
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2290 2269
Size: 21 5272
BstYI r'GATC_y
Cuts at: 1972 2623 2990 3375 3392 4844 4930 4942 5288
Size: 651 367 385 17 1452 86 12 346
Cuts at: 5288 1972
Size: 1977
Fragments arranged by size:
1977 1452 651 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2526 513
Size: 552 939 306 216 3280
Fragments arranged by size:
3280 939 552 306 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 2809 3603 3623 2223
Size: 586 794 20 3893
Fragments arranged by size:
3893 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2409 2577
Size: 54 1425 50 275 18 228 351 168
Cuts at: 2577 2740 3629 5258 8
Size: 163 889 1629 43
Fragments arranged by size:
1629 1425 889 351 275 228 168 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3223 1353
Size: 1870 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 2102 3880 4254 4400 387
Size: 1064 138 495 18 1778 374 146 1280
Fragments arranged by size:
1778 1280 1064 495 374 146 138 18
EcoICRI GAG'CTC
Cuts at: 2406 2538 2406
Size: 132 5161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2501 3356 3953 4740 5008 1239
Size: 393 437 432 855 597 787 268 1524
Fragments arranged by size:
1524 855 787 597 437 432 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2321 2294
Size: 27 5266
EcoO109I rG'GnC_Cy
Cuts at: 2110 2299 2110
Size: 189 5104
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 2546 3799 2546
Size: 1253 4040
HaeII r_GCGC'y
Cuts at: 1678 2520 4440 1678
Size: 842 1920 2531
Fragments arranged by size:
2531 1920 842
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 3934 3966 4008
Size: 32 135 32 977 32 1353 32 42
Cuts at: 4008 4040 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 977 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KasI G'GCGC_C
Cuts at: 2516 2516
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 10
Size: 54 1425 343 228 3243
Fragments arranged by size:
3243 1425 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3610 3769 518
Size: 648 2085 359 159 2042
Fragments arranged by size:
2085 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2240 2506 2563 2709 3386 4534 4779
Size: 441 193 266 57 146 677 1148 245
Cuts at: 4779 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 441 266 245 193 146
57
NarI GG'CG_CC
Cuts at: 2517 2517
Size: 5293
NcoI C'CATG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 3496
Fragments arranged by size:
3496 939 552 306
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2651 4196 1132
Size: 97 1422 1545 2229
Fragments arranged by size:
2229 1545 1422 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5293
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5293
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2121 2322 2645 830
Size: 1291 201 323 3478
Fragments arranged by size:
3478 1291 323 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
PvuII CAG'CTG
Cuts at: 2047 2240 2506 2563 2047
Size: 193 266 57 4777
Fragments arranged by size:
4777 266 193 57
SacI G_AGCT'C
Cuts at: 2408 2540 2408
Size: 132 5161
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2117 2318 2641 3776 4457 4648 826
Size: 219 1072 201 323 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 323 219 201 191
SfoI GGC'GCC
Cuts at: 2518 2518
Size: 5293
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2620 1872
Size: 748 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2584 3397 4298 4560 820
Size: 197 926 641 813 901 262 1553
Fragments arranged by size:
1553 926 901 813 641 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2226 2310 2732 513
Size: 552 939 78 144 84 422 3074
Fragments arranged by size:
3074 939 552 422 144 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5253 5285 5253
Size: 32 5261
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2618 1870
Size: 748 4545
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2374 3101 276
Size: 53 83 186 936 840 727 2468
Fragments arranged by size:
2468 936 840 727 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI