Restriction map for
pFN21AE0996
(Circular) (Six-base) MAPSORT of: pFN21AE0996.seq Check: 1113 from: 1 to: 6682
pFN21AE0996 6682 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4492 278
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4025 1937
Size: 2088 4594
Acc65I G'GTAC_C
Cuts at: 4003 4003
Size: 6682
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2376 2835 3339 3855 4745 6129 6397
Size: 425 319 459 504 516 890 1384 268
Cuts at: 6397 1632
Size: 1917
Fragments arranged by size:
1917 1384 890 516 504 459 425 319
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 5581 6558 1203
Size: 4378 977 1327
Fragments arranged by size:
4378 1327 977
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 2868 5411 2868
Size: 2543 4139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3117 1184
Size: 1933 4749
AlwNI CAG_nnn'CTG
Cuts at: 2957 3496 4425 5997 2957
Size: 539 929 1572 3642
Fragments arranged by size:
3642 1572 929 539
ApaLI G'TGCA_C
Cuts at: 2105 4739 5895 2105
Size: 2634 1156 2892
Fragments arranged by size:
2892 2634 1156
ApoI r'AATT_y
Cuts at: 1493 2277 2502 3326 3677 3771 3939 3991 4217
Size: 784 225 824 351 94 168 52 226
Cuts at: 4217 4369 6478 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 824 784 351 226 225 168
152 94 52
AseI AT'TA_AT
Cuts at: 160 1032 5236 160
Size: 872 4204 1606
Fragments arranged by size:
4204 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 4007 1870
Size: 73 2064 4545
Fragments arranged by size:
4545 2064 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2395 2428 2395
Size: 33 6649
BamHI G'GATC_C
Cuts at: 4012 4379 4012
Size: 367 6315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4003 5458 618
Size: 325 217 627 2216 1455 1842
Fragments arranged by size:
2216 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3078 4001 1391
Size: 1687 923 4072
Fragments arranged by size:
4072 1687 923
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 2558 2829 928
Size: 971 436 223 271 4781
Fragments arranged by size:
4781 971 436 271 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4873 4907 1903
Size: 34 2936 34 3678
Fragments arranged by size:
3678 2936 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3170 4574 5784 3170
Size: 1404 1210 4068
Fragments arranged by size:
4068 1404 1210
BclI T'GATC_A
Cuts at: 1548 3297 1548
Size: 1749 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5293 136
Size: 107 122 71 631 605 3621 1525
Fragments arranged by size:
3621 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2592 6677 2592
Size: 4085 2597
BlpI GC'TnA_GC
Cuts at: 2877 4099 2877
Size: 1222 5460
Bme1580I G_kGCm'C
Cuts at: 1790 2109 4743 5899 1790
Size: 319 2634 1156 2573
Fragments arranged by size:
2634 2573 1156 319
BmgBI CAC'GTC
Cuts at: 1318 3283 1318
Size: 1965 4717
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4413 5371 442
Size: 416 53 83 399 24 2996 958 1753
Fragments arranged by size:
2996 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2211 2914 3560 5342 1239
Size: 803 169 703 646 1782 2579
Fragments arranged by size:
2579 1782 803 703 646 169
Bpu10I CC'TnA_GC
Cuts at: 2616 3072 2616
Size: 456 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2970 4807 5672 5970 2970
Size: 1837 865 298 3682
Fragments arranged by size:
3682 1837 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3558 5345 882
Size: 617 2059 1787 2219
Fragments arranged by size:
2219 2059 1787 617
BsaAI yAC'GTr
Cuts at: 493 1204 4473 493
Size: 711 3269 2702
Fragments arranged by size:
3269 2702 711
BsaBI GATnn'nnATC
Cuts at: 3181 6676 3181
Size: 3495 3187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3390 4489 4871 5506
Size: 53 83 186 936 1857 1099 382 635
Cuts at: 5506 275
Size: 1451
Fragments arranged by size:
1857 1451 1099 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2400 2547 3687 3700 5115 5513 5787 5934 6345
Size: 147 1140 13 1415 398 274 147 411
Cuts at: 6345 2400
Size: 2737
Fragments arranged by size:
2737 1415 1140 411 398 274 147 147
13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2330 2360 1178
Size: 30 165 30 696 30 201 30 5500
Fragments arranged by size:
5500 696 201 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2597 1186
Size: 1028 383 5271
Fragments arranged by size:
5271 1028 383
BseYI C'CCAG_C
Cuts at: 3226 5885 6381 3226
Size: 2659 496 3527
Fragments arranged by size:
3527 2659 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3379 3398 1163
Size: 573 1643 19 4447
Fragments arranged by size:
4447 1643 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4132 4893 5921 1540
Size: 275 186 2131 761 1028 2301
Fragments arranged by size:
2301 2131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2767 3078 4743 4828 5899 2109
Size: 658 311 1665 85 1071 2892
Fragments arranged by size:
2892 1665 1071 658 311 85
BsmI GAATG_Cn'
Cuts at: 2695 3157 4193 4286 2695
Size: 462 1036 93 5091
Fragments arranged by size:
5091 1036 462 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3854 3854
Size: 6682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2767 3078 4001 4743 4828 5899
Size: 399 319 658 311 923 742 85 1071
Cuts at: 5899 1391
Size: 2174
Fragments arranged by size:
2174 1071 923 742 658 399 319 311
85
BspEI T'CCGG_A
Cuts at: 6345 6345
Size: 6682
BspHI T'CATG_A
Cuts at: 3348 4571 6301 3348
Size: 1223 1730 3729
Fragments arranged by size:
3729 1730 1223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4037 844
Size: 3193 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4569 1394
Size: 429 2746 3507
Fragments arranged by size:
3507 2746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3831 5177 5351 65
Size: 1183 2583 1346 174 1396
Fragments arranged by size:
2583 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5326 5513 1700
Size: 110 3516 187 2869
Fragments arranged by size:
3516 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3894 96
Size: 3798 2884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 2766 4742 5754 2766
Size: 1976 1012 3694
Fragments arranged by size:
3694 1976 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6682
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6682
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 3690 2269
Size: 1421 5261
BstYI r'GATC_y
Cuts at: 1972 2592 4012 4379 4764 4781 6233 6319 6331
Size: 620 1420 367 385 17 1452 86 12
Cuts at: 6331 6677 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1420 620 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2580 3461 2580
Size: 881 5801
BtgI C'CryG_G
Cuts at: 513 1065 2004 2753 3528 513
Size: 552 939 749 775 3667
Fragments arranged by size:
3667 939 775 749 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5943
Fragments arranged by size:
5943 683 56
BtsI GCAGTG_nn'
Cuts at: 2019 3162 4198 4992 5012 2019
Size: 1143 1036 794 20 3689
Fragments arranged by size:
3689 1143 1036 794 20
ClaI AT'CG_AT
Cuts at: 1554 2448 1554
Size: 894 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6647
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5689 809
Size: 507 4373 1802
Fragments arranged by size:
4373 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3285 3435
Size: 54 1425 50 275 18 177 1278 150
Cuts at: 3435 4129 5018 6647 8
Size: 694 889 1629 43
Fragments arranged by size:
1629 1425 1278 889 694 275 177 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 2242 2250 2571 4612 1353
Size: 790 99 8 321 2041 3423
Fragments arranged by size:
3423 2041 790 321 99 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5269 5643 5789 387
Size: 1064 138 3680 374 146 1280
Fragments arranged by size:
3680 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3076 3076
Size: 6682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2057 2211 2376 2835 2914 3339
Size: 393 410 15 154 165 459 79 425
Cuts at: 3339 3560 3855 4745 5342 6129 6397 1239
Size: 221 295 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 459 425 410 393
295 268 221 165 154 79 15
EcoO109I rG'GnC_Cy
Cuts at: 2330 2980 3205 2330
Size: 650 225 5807
Fragments arranged by size:
5807 650 225
EcoRI G'AATT_C
Cuts at: 3326 3991 4369 3326
Size: 665 378 5639
Fragments arranged by size:
5639 665 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2316 2316
Size: 6682
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 2089 3409 5829 1678
Size: 411 1320 2420 2531
Fragments arranged by size:
2531 2420 1320 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2854 2886 3962 3994 5323
Size: 32 135 32 1282 32 1076 32 1329
Cuts at: 5323 5355 5397 5429 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 1282 1076 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4026 4272 669
Size: 988 281 2088 246 3079
Fragments arranged by size:
3079 2088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2524 748
Size: 1776 4906
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KpnI G_GTAC'C
Cuts at: 4007 4007
Size: 6682
MfeI C'AATT_G
Cuts at: 4281 4281
Size: 6682
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5796 5980 1305
Size: 4491 184 2007
Fragments arranged by size:
4491 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3287 3437 10
Size: 54 1425 343 1455 150 3255
Fragments arranged by size:
3255 1455 1425 343 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2834 3293 4640 4999 5158 518
Size: 648 1518 150 459 1347 359 159 2042
Fragments arranged by size:
2042 1518 1347 648 459 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2707 3496 4098 4775 5923 6168 1606
Size: 1101 789 602 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 789 677 602 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2753 3528 513
Size: 552 939 749 775 3667
Fragments arranged by size:
3667 939 775 749 552
NdeI CA'TA_TG
Cuts at: 387 3796 387
Size: 3409 3273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 2683 4040 5585 1132
Size: 97 1454 1357 1545 2229
Fragments arranged by size:
2229 1545 1454 1357 97
PasI CC'CwG_GG
Cuts at: 1348 3781 1348
Size: 2433 4249
PciI A'CATG_T
Cuts at: 5581 5581
Size: 6682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 5284
PfoI T'CCnGG_A
Cuts at: 3642 3642
Size: 6682
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2980 3205 2980
Size: 225 6457
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 4252 4252
Size: 6682
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2149 2181 3707 3739 2149
Size: 32 1526 32 5092
Fragments arranged by size:
5092 1526 32 32
PstI C_TGCA'G
Cuts at: 830 3106 3169 4034 830
Size: 2276 63 865 3478
Fragments arranged by size:
3478 2276 865 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 2707 3496 2707
Size: 789 5893
SacI G_AGCT'C
Cuts at: 3078 3078
Size: 6682
SalI G'TCGA_C
Cuts at: 1936 4024 1936
Size: 2088 4594
SanDI GG'GwC_CC
Cuts at: 2980 2980
Size: 6682
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SfcI C'TryA_G
Cuts at: 826 1045 2702 3102 3165 3853 4030 5165 5846
Size: 219 1657 400 63 688 177 1135 681
Cuts at: 5846 6037 826
Size: 191 1471
Fragments arranged by size:
1657 1471 1135 688 681 400 219 191
177 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 4009 1872
Size: 2137 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2985 4786 5687 5949 820
Size: 197 926 1042 1801 901 262 1553
Fragments arranged by size:
1801 1553 1042 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 2726 3955 4606 5
Size: 47 2674 1229 651 2081
Fragments arranged by size:
2674 2081 1229 651 47
StuI AGG'CCT
Cuts at: 1517 3171 3843 3859 1517
Size: 1654 672 16 4340
Fragments arranged by size:
4340 1654 672 16
StyI C'CwwG_G
Cuts at: 513 1065 2004 2753 3528 3838 4121 513
Size: 552 939 749 775 310 283 3074
Fragments arranged by size:
3074 939 775 749 552 310 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4709 4726 4879 5064 5509 325
Size: 1255 3129 17 153 185 445 1498
Fragments arranged by size:
3129 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3429 3894 4928
Size: 274 80 33 51 1443 1452 465 1034
Cuts at: 4928 6640 96
Size: 1712 138
Fragments arranged by size:
1712 1452 1443 1034 465 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 3690 3722 6642 6674 2099
Size: 32 1559 32 2920 32 2107
Fragments arranged by size:
2920 2107 1559 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 3958 4018 3958
Size: 60 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 3169 3535 3640 2488
Size: 681 366 105 5530
Fragments arranged by size:
5530 681 366 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 4007 1870
Size: 2137 4545
XmnI GAAnn'nnTTC
Cuts at: 4811 4811
Size: 6682
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4490 276
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI