Restriction map for
pFN21AE0995
(Circular) (Six-base) MAPSORT of: pFN21AE0995.seq Check: 8683 from: 1 to: 6649
pFN21AE0995 6649 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3874 3874
Size: 6649
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3264 3792 3992 1937
Size: 1327 528 200 4594
Fragments arranged by size:
4594 1327 528 200
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2432 3812 4712 6096 6364 1632
Size: 416 384 1380 900 1384 268 1917
Fragments arranged by size:
1917 1384 1380 900 416 384 268
AfeI AGC'GCT
Cuts at: 3806 3806
Size: 6649
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 3609 5548 6525 1203
Size: 2406 1939 977 1327
Fragments arranged by size:
2406 1939 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 2100 2163 1166
Size: 934 63 5652
Fragments arranged by size:
5652 934 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2118 3165 1184
Size: 934 1047 4668
Fragments arranged by size:
4668 1047 934
AlwNI CAG_nnn'CTG
Cuts at: 2458 2550 4392 5964 2458
Size: 92 1842 1572 3143
Fragments arranged by size:
3143 1842 1572 92
ApaI G_GGCC'C
Cuts at: 2080 2513 2080
Size: 433 6216
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 3573 3958 4184 4336 6445 1493
Size: 2080 385 226 152 2109 1697
Fragments arranged by size:
2109 2080 1697 385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2243 2401 2564 2874 3974 1870
Size: 73 130 170 158 163 310 1100 4545
Fragments arranged by size:
4545 1100 310 170 163 158 130 73
BamHI G'GATC_C
Cuts at: 3979 4346 3979
Size: 367 6282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2226 3126 3970 5425 618
Size: 325 217 627 439 900 844 1455 1842
Fragments arranged by size:
1842 1455 900 844 627 439 325 217
BanII G_rGCy'C
Cuts at: 1391 2080 2513 2979 3360 3693 3968 1391
Size: 689 433 466 381 333 275 4072
Fragments arranged by size:
4072 689 466 433 381 333 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5678
BbvCI CC'TCA_GC
Cuts at: 2120 2645 2774 2120
Size: 525 129 5995
Fragments arranged by size:
5995 525 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3379 3413 4840 4874 1903
Size: 34 1442 34 1427 34 3678
Fragments arranged by size:
3678 1442 1427 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5751 4541
Size: 1210 5439
BclI T'GATC_A
Cuts at: 1548 3216 3375 1548
Size: 1668 159 4822
Fragments arranged by size:
4822 1668 159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3010 5260 136
Size: 107 122 71 631 605 1338 2250 1525
Fragments arranged by size:
2250 1525 1338 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3210 3766 6644 3210
Size: 556 2878 3215
Fragments arranged by size:
3215 2878 556
BlpI GC'TnA_GC
Cuts at: 4066 4066
Size: 6649
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2107 2513 4710 5866 1790
Size: 290 27 406 2197 1156 2573
Fragments arranged by size:
2573 2197 1156 406 290 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2310 3748 4380
Size: 416 53 83 399 24 893 1438 632
Cuts at: 4380 5338 442
Size: 958 1753
Fragments arranged by size:
1753 1438 958 893 632 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2493 3249 2493
Size: 756 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 2943 3317 3620 5309 1239
Size: 1241 463 374 303 1689 2579
Fragments arranged by size:
2579 1689 1241 463 374 303
Bpu10I CC'TnA_GC
Cuts at: 2120 2645 2712 2774 2120
Size: 525 67 62 5995
Fragments arranged by size:
5995 525 67 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2622 3164 3422 4774 5639 5937 2622
Size: 542 258 1352 865 298 3334
Fragments arranged by size:
3334 1352 865 542 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2148 2165 2171 2565 2644 3128 3309
Size: 617 649 17 6 394 79 484 181
Cuts at: 3309 3520 5312 882
Size: 211 1792 2219
Fragments arranged by size:
2219 1792 649 617 484 394 211 181
79 17 6
BsaAI yAC'GTr
Cuts at: 493 1204 3304 3562 4440 493
Size: 711 2100 258 878 2702
Fragments arranged by size:
2702 2100 878 711 258
BsaBI GATnn'nnATC
Cuts at: 3158 3215 6643 3158
Size: 57 3428 3164
Fragments arranged by size:
3428 3164 57
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3327 4456 4838 5473
Size: 53 83 186 936 1794 1129 382 635
Cuts at: 5473 275
Size: 1451
Fragments arranged by size:
1794 1451 1129 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3912 5082 5480 5754 5901 6312 3912
Size: 1170 398 274 147 411 4249
Fragments arranged by size:
4249 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 5218
Fragments arranged by size:
5218 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2137 2261 2662 2780 2892 3327 1186
Size: 951 124 401 118 112 435 4508
Fragments arranged by size:
4508 951 435 401 124 118 112
BseYI C'CCAG_C
Cuts at: 2091 2654 3014 3709 3893 5852 6348 2091
Size: 563 360 695 184 1959 496 2392
Fragments arranged by size:
2392 1959 695 563 496 360 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3326 1163
Size: 573 1590 4486
Fragments arranged by size:
4486 1590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 4099 4860 5888 1540
Size: 275 186 1021 1077 761 1028 2301
Fragments arranged by size:
2301 1077 1028 1021 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2844 3693 4710 4795 5866 2844
Size: 849 1017 85 1071 3627
Fragments arranged by size:
3627 1071 1017 849 85
BsmI GAATG_Cn'
Cuts at: 2034 4160 4253 2034
Size: 2126 93 4430
Fragments arranged by size:
4430 2126 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2107 2513 2844 2979 3360 3693
Size: 399 290 27 406 331 135 381 333
Cuts at: 3693 3968 4710 4795 5866 1391
Size: 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 381 333 331
290 275 135 85 27
BspEI T'CCGG_A
Cuts at: 3912 6312 3912
Size: 2400 4249
BspHI T'CATG_A
Cuts at: 3372 4538 6268 3372
Size: 1166 1730 3753
Fragments arranged by size:
3753 1730 1166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2965 3874 3931 4004 844
Size: 2121 909 57 73 3489
Fragments arranged by size:
3489 2121 909 73 57
BsrBI CCG'CTC
Cuts at: 1394 1823 3010 4536 1394
Size: 429 1187 1526 3507
Fragments arranged by size:
3507 1526 1187 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 5144 5318 65
Size: 1183 1797 2099 174 1396
Fragments arranged by size:
2099 1797 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3777 5293 5480 1700
Size: 110 1967 1516 187 2869
Fragments arranged by size:
2869 1967 1516 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 4709 5721 4709
Size: 1012 5637
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2576 794
Size: 1782 4867
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 2165 2494 2165
Size: 329 6320
BstYI r'GATC_y
Cuts at: 1972 2238 3210 3766 3979 4346 4731 4748 6200
Size: 266 972 556 213 367 385 17 1452
Cuts at: 6200 6286 6298 6644 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 972 556 385 367 346 266
213 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3029 3036 513
Size: 552 939 1025 7 4126
Fragments arranged by size:
4126 1025 939 552 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3167 487
Size: 683 56 1941 3969
Fragments arranged by size:
3969 1941 683 56
BtsI GCAGTG_nn'
Cuts at: 4165 4959 4979 4165
Size: 794 20 5835
Fragments arranged by size:
5835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2803 2838 3501 3536 529
Size: 35 2239 35 663 35 3642
Fragments arranged by size:
3642 2239 663 35 35 35
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5656 809
Size: 507 4340 1802
Fragments arranged by size:
4340 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 4096 4985
Size: 54 1425 50 275 18 1249 1017 889
Cuts at: 4985 6614 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1249 1017 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2343 2877 2988 4579 1353
Size: 671 319 534 111 1591 3423
Fragments arranged by size:
3423 1591 671 534 319 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5236 5610 5756 387
Size: 1064 138 3647 374 146 1280
Fragments arranged by size:
3647 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3691 3691
Size: 6649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2432 2480 2943 3317 3620 3812
Size: 393 416 384 48 463 374 303 192
Cuts at: 3812 4712 5309 6096 6364 1239
Size: 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 597 463 416 393 384
374 303 268 192 48
EcoO109I rG'GnC_Cy
Cuts at: 2077 2274 2509 2737 2871 3433 3549 2077
Size: 197 235 228 134 562 116 5177
Fragments arranged by size:
5177 562 235 228 197 134 116
EcoRI G'AATT_C
Cuts at: 3958 4336 3958
Size: 378 6271
FspI TGC'GCA
Cuts at: 5155 5155
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 3808 5796 1678
Size: 2130 1988 2531
Fragments arranged by size:
2531 2130 1988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3461 3493 3734 3766 3929
Size: 32 135 32 1889 32 241 32 163
Cuts at: 3929 3961 5290 5322 5364 5396 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1889 1329 241 163 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3993 4239 669
Size: 988 281 2055 246 3079
Fragments arranged by size:
3079 2055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3002 5763 5947 1305
Size: 1697 2761 184 2007
Fragments arranged by size:
2761 2007 1697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4827
Fragments arranged by size:
4827 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2100 2163 3377 4607 4966 5125
Size: 648 873 61 63 1214 1230 359 159
Cuts at: 5125 518
Size: 2042
Fragments arranged by size:
2042 1230 1214 873 648 359 159 63
61
MspA1I CmG'CkG
Cuts at: 1606 2163 3018 3709 3851 4065 4742 5890 6135
Size: 557 855 691 142 214 677 1148 245
Cuts at: 6135 1606
Size: 2120
Fragments arranged by size:
2120 1148 855 691 677 557 245 214
142
NcoI C'CATG_G
Cuts at: 513 1065 2004 3036 513
Size: 552 939 1032 4126
Fragments arranged by size:
4126 1032 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 3409 3521 3613 4007 5552 1132
Size: 97 2180 112 92 394 1545 2229
Fragments arranged by size:
2229 2180 1545 394 112 97 92
PasI CC'CwG_GG
Cuts at: 1348 2331 1348
Size: 983 5666
PciI A'CATG_T
Cuts at: 3609 5548 3609
Size: 1939 4710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6649
PfoI T'CCnGG_A
Cuts at: 3294 3351 3294
Size: 57 6592
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2274 2737 2871 3433 3549 2274
Size: 463 134 562 116 5374
Fragments arranged by size:
5374 562 463 134 116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6649
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PspOMI G'GGCC_C
Cuts at: 2076 2509 2076
Size: 433 6216
PstI C_TGCA'G
Cuts at: 830 2195 2555 4001 830
Size: 1365 360 1446 3478
Fragments arranged by size:
3478 1446 1365 360
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 3709 3851 3709
Size: 142 6507
SacI G_AGCT'C
Cuts at: 3693 3693
Size: 6649
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SanDI GG'GwC_CC
Cuts at: 3549 3549
Size: 6649
SapI GCTCTTCn'nnn_
Cuts at: 2024 2024
Size: 6649
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SfcI C'TryA_G
Cuts at: 826 1045 2046 2191 2551 3997 5132 5813 6004
Size: 219 1001 145 360 1446 1135 681 191
Cuts at: 6004 826
Size: 1471
Fragments arranged by size:
1471 1446 1135 1001 681 360 219 191
145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 2075 3976 1872
Size: 203 1901 4545
Fragments arranged by size:
4545 1901 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2637 3179 3437 4753 5654 5916
Size: 197 926 694 542 258 1316 901 262
Cuts at: 5916 820
Size: 1553
Fragments arranged by size:
1553 1316 926 901 694 542 262 258
197
SnaBI TAC'GTA
Cuts at: 493 3562 4440 493
Size: 3069 878 2702
Fragments arranged by size:
3069 2702 878
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SrfI GCCC'GGGC
Cuts at: 2075 2075
Size: 6649
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 3146 1517
Size: 1629 5020
StyI C'CwwG_G
Cuts at: 513 1065 2004 2114 2724 3036 4088 513
Size: 552 939 110 610 312 1052 3074
Fragments arranged by size:
3074 1052 939 610 552 312 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 4676 4693 4846 5031 5476 325
Size: 1255 609 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 609 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4895 6607 96
Size: 274 80 33 51 1443 2918 1712 138
Fragments arranged by size:
2918 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 3588 3620 6609 6641 2579
Size: 32 977 32 2989 32 2587
Fragments arranged by size:
2989 2587 977 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6649
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2662 2662
Size: 6649
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 2073 3974 1870
Size: 203 1901 4545
Fragments arranged by size:
4545 1901 203
XmnI GAAnn'nnTTC
Cuts at: 2751 4778 2751
Size: 2027 4622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SwaI